JP5249194B2 - 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現 - Google Patents

非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現 Download PDF

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JP5249194B2
JP5249194B2 JP2009500449A JP2009500449A JP5249194B2 JP 5249194 B2 JP5249194 B2 JP 5249194B2 JP 2009500449 A JP2009500449 A JP 2009500449A JP 2009500449 A JP2009500449 A JP 2009500449A JP 5249194 B2 JP5249194 B2 JP 5249194B2
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trna
amino acid
protein
unnatural amino
amino acids
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ジャンギュン・ワング
ピーター・ジー．シュルツ
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ザ スクリプス リサーチ インスティチュート
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2009500449A 2006-03-16 2007-03-13 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現 Expired - Fee Related JP5249194B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US78327206P 2006-03-16 2006-03-16
US60/783,272 2006-03-16
US86145606P 2006-11-28 2006-11-28
US60/861,456 2006-11-28
PCT/US2007/006382 WO2007109035A2 (en) 2006-03-16 2007-03-13 Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Publications (3)

Publication Number Publication Date
JP2009529877A JP2009529877A (ja) 2009-08-27
JP2009529877A5 JP2009529877A5 (https=) 2010-04-30
JP5249194B2 true JP5249194B2 (ja) 2013-07-31

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JP2009500449A Expired - Fee Related JP5249194B2 (ja) 2006-03-16 2007-03-13 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現

Country Status (12)

Country Link
US (3) US7790847B2 (https=)
EP (1) EP1994165B1 (https=)
JP (1) JP5249194B2 (https=)
KR (1) KR20080113226A (https=)
CN (1) CN101405401B (https=)
AU (1) AU2007227592B2 (https=)
BR (1) BRPI0709603A2 (https=)
CA (1) CA2638772A1 (https=)
IL (1) IL193722A0 (https=)
MX (1) MX2008011669A (https=)
RU (1) RU2464315C2 (https=)
WO (1) WO2007109035A2 (https=)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7083970B2 (en) * 2001-04-19 2006-08-01 The Scripps Research Institute Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
WO2004094593A2 (en) 2003-04-17 2004-11-04 The Scripps Research Institute Expanding the eukaryotic genetic code
WO2005019415A2 (en) 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
EP1807515A4 (en) 2004-10-27 2008-06-18 Scripps Research Inst Orthogonal translation components for the in vivo incorporation of unnatural amino acids
US7960141B2 (en) * 2006-03-09 2011-06-14 The Scripps Research Institute Systems for the expression of orthogonal translation components in eubacterial host cells
KR20080113226A (ko) 2006-03-16 2008-12-29 더 스크립스 리서치 인스티튜트 비천연 아미노산 페닐셀레노시스테인을 함유하는 단백질의 유전자적으로 프로그램된 발현
US7807411B2 (en) * 2006-05-23 2010-10-05 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
EP2052083B1 (en) * 2006-09-21 2014-01-08 The Scripps Research Institute Genetically programmed expression of selectively sulfated proteins in eubacteria
JP2010506591A (ja) 2006-10-18 2010-03-04 ザ スクリップス リサーチ インスティテュート 哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み
MX2010004464A (es) 2007-10-25 2010-06-07 Scripps Research Inst Incorporacion genetica de 3-aminotirosina a reductasas.
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
US9150849B2 (en) 2007-11-02 2015-10-06 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
AU2008335778B2 (en) 2007-12-11 2014-04-10 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris
US8592199B2 (en) 2007-12-28 2013-11-26 David H. Ardell Genetic encryption
CA2710683A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
CN101970005A (zh) * 2008-02-08 2011-02-09 斯克利普斯研究院 用遗传编码的非天然氨基酸打破免疫耐受
CN102307905B (zh) 2008-12-10 2015-11-25 斯克利普斯研究院 利用化学反应性非天然氨基酸产生载体-肽偶联物
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
US8658394B2 (en) * 2011-02-02 2014-02-25 Waterstone Pharmaceuticals Methods, cells and systems for incorporating non-canonical amino acids into proteins
CN103215235B (zh) * 2012-01-18 2014-11-05 中国科学院生物物理研究所 3-咪唑基酪氨酸翻译系统及其应用
CN103571804B (zh) * 2012-08-10 2015-08-12 中国科学院生物物理研究所 3-吡唑基酪氨酸翻译系统及其应用
JP6421122B2 (ja) 2012-10-12 2018-11-07 ストロ バイオファーマ， インコーポレイテッド 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化
CN103820410B (zh) * 2012-11-16 2016-03-30 中国科学院生物物理研究所 3-甲硫酪氨酸翻译系统及其应用
CN104004723B (zh) * 2013-02-22 2016-08-17 中国科学院生物物理研究所 3,5-二氟代酪氨酸翻译系统及其应用
CN104059891B (zh) * 2013-03-22 2016-08-17 中国科学院生物物理研究所 8-羟基喹啉丙氨酸翻译系统及其应用
CN105792851B (zh) * 2013-09-13 2023-10-10 斯克利普斯研究所 修饰的治疗剂及其组合物
AU2014364589B2 (en) 2013-12-18 2020-02-27 The California Institute For Biomedical Research Modified therapeutic agents, stapled peptide lipid conjugates, and compositions thereof
CN109414451B (zh) * 2016-05-16 2022-08-12 德克萨斯大学系统董事会 用于作为纳米粒递送tRNA的组合物及其使用方法
AU2017386533B2 (en) 2016-12-29 2024-05-09 Illumina, Inc. Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments
AU2020397917A1 (en) 2019-12-04 2022-06-23 The Scripps Research Institute GLP2 receptor agonists and methods of use
CN114644581B (zh) * 2021-01-04 2024-07-16 北京惠大生物科技有限公司 含芳基硫酚或芳基硒酚经修饰的氨基酸、重组蛋白及其生物合成方法及应用
MX2023014771A (es) 2021-06-09 2024-03-19 Scripps Research Inst Conjugados peptidicos duales gip/glp-1 de accion prolongada y metodos de uso.
CN118389493B (zh) * 2024-05-10 2025-04-11 北京大学 一种定点修饰的工程化tRNA及其应用

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI941169L (fi) * 1992-07-13 1994-05-04 Eukarion Inc Lipidisoitujen proteiinien transvaskulaarinen ja intracellulaarinen antaminen
US7083970B2 (en) 2001-04-19 2006-08-01 The Scripps Research Institute Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
MXPA05003978A (es) 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
ES2324608T3 (es) * 2002-10-16 2009-08-11 The Scripps Research Institute Incorporacion especifica de sitio de cetoaminoacidos en proteinas.
WO2004058946A2 (en) 2002-12-22 2004-07-15 The Scripps Research Institute Protein arrays
WO2004094593A2 (en) 2003-04-17 2004-11-04 The Scripps Research Institute Expanding the eukaryotic genetic code
JP5275566B2 (ja) 2003-06-18 2013-08-28 ザ スクリプス リサーチ インスティチュート 反応性非天然アミノ酸遺伝コード付加
US20060160175A1 (en) 2003-07-07 2006-07-20 The Scripps Research Institute Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
WO2005019415A2 (en) 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
JP5657850B2 (ja) * 2003-10-14 2015-01-21 ザ スクリプス リサーチ インスティテュート 酸化還元機能性アミノ酸のタンパク質への部位特異的組み入れ
AU2004321117B2 (en) 2003-12-18 2010-09-02 The Scripps Research Institute Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
US20050287639A1 (en) * 2004-05-17 2005-12-29 California Institute Of Technology Methods of incorporating amino acid analogs into proteins
AU2005248392A1 (en) 2004-05-25 2005-12-08 Irm, Llc Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
CA2580840A1 (en) 2004-09-21 2006-03-30 The Scripps Research Institute In vivo incorporation of alkynyl amino acids into proteins in eubacteria
US20090181858A1 (en) 2004-09-21 2009-07-16 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
US20080171317A1 (en) 2004-09-22 2008-07-17 The Scripps Research Institute Site-Specific Labeling of Proteins for Nmr Studies
JP2008516637A (ja) 2004-10-20 2008-05-22 ザ スクリップス リサーチ インスティテュート 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み
EP1807515A4 (en) 2004-10-27 2008-06-18 Scripps Research Inst Orthogonal translation components for the in vivo incorporation of unnatural amino acids
US8367588B2 (en) 2005-10-12 2013-02-05 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
US7960141B2 (en) 2006-03-09 2011-06-14 The Scripps Research Institute Systems for the expression of orthogonal translation components in eubacterial host cells
KR20080113226A (ko) 2006-03-16 2008-12-29 더 스크립스 리서치 인스티튜트 비천연 아미노산 페닐셀레노시스테인을 함유하는 단백질의 유전자적으로 프로그램된 발현

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AU2007227592A1 (en) 2007-09-27
US7790847B2 (en) 2010-09-07
WO2007109035A2 (en) 2007-09-27
WO2007109035A3 (en) 2008-10-16
EP1994165A2 (en) 2008-11-26
RU2464315C2 (ru) 2012-10-20
CN101405401A (zh) 2009-04-08
US20120282668A1 (en) 2012-11-08
US20070238152A1 (en) 2007-10-11
KR20080113226A (ko) 2008-12-29
CA2638772A1 (en) 2007-09-27
US8217145B2 (en) 2012-07-10
JP2009529877A (ja) 2009-08-27
AU2007227592B2 (en) 2011-11-24
EP1994165B1 (en) 2014-11-05
US20090209000A1 (en) 2009-08-20
US8394605B2 (en) 2013-03-12
RU2008135114A (ru) 2010-04-27
MX2008011669A (es) 2008-09-22
IL193722A0 (en) 2011-08-01
EP1994165A4 (en) 2012-11-07
CN101405401B (zh) 2013-03-27
BRPI0709603A2 (pt) 2011-07-19

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