JP5235905B2 - 核酸配列の検出方法 - Google Patents
核酸配列の検出方法 Download PDFInfo
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- JP5235905B2 JP5235905B2 JP2009551268A JP2009551268A JP5235905B2 JP 5235905 B2 JP5235905 B2 JP 5235905B2 JP 2009551268 A JP2009551268 A JP 2009551268A JP 2009551268 A JP2009551268 A JP 2009551268A JP 5235905 B2 JP5235905 B2 JP 5235905B2
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- primer
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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| GB0703996D0 (en) * | 2007-03-01 | 2007-04-11 | Oxitec Ltd | Nucleic acid detection |
| GB0909333D0 (en) * | 2009-06-01 | 2009-07-15 | Fu Guoliang | Multiplex amplification and detection |
| US20100285537A1 (en) * | 2009-04-02 | 2010-11-11 | Fluidigm Corporation | Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation |
| EP2516679B1 (en) * | 2009-12-21 | 2017-08-23 | Seegene, Inc. | Tsg primer target detection |
| DE102010003781B4 (de) | 2010-04-08 | 2012-08-16 | Aj Innuscreen Gmbh | Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen |
| CN101871007B (zh) * | 2010-05-07 | 2014-04-30 | 无锡锐奇基因生物科技有限公司 | 用标记探针进行检测和熔解曲线分析的方法 |
| GB201017978D0 (en) | 2010-10-25 | 2010-12-08 | Oxitec Ltd | Multiplex amplification and detection |
| WO2012061412A1 (en) * | 2010-11-01 | 2012-05-10 | Gen-Probe Incorporated | Integrated capture and amplification of target nucleic acid for sequencing |
| US8815509B2 (en) * | 2011-06-14 | 2014-08-26 | University Of Southern California | Fluorescence-based assay for the rapid detection and quantification of deoxyribonucleoside triphosphates |
| CN102242211A (zh) * | 2011-07-08 | 2011-11-16 | 无锡锐奇基因生物科技有限公司 | 检测突变核酸序列的方法及试剂盒 |
| WO2013093432A1 (en) * | 2011-12-19 | 2013-06-27 | David Kelly | Method for enriching and detection of variant target nucleic acids |
| JP6200948B2 (ja) * | 2012-05-25 | 2017-09-20 | ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | マイクロ流体デバイス |
| CA2917627A1 (en) * | 2013-07-18 | 2015-01-22 | President And Fellows Of Harvard College | Specific nucleic acid amplification with compounded selectivity |
| GB201312995D0 (en) * | 2013-07-19 | 2013-09-04 | Atlas Genetics Ltd | Methods and kits for specific nucleic acid amplification and detection |
| CN104561248A (zh) * | 2013-10-22 | 2015-04-29 | 常州金麦格生物技术有限公司 | 用于检测靶核酸的引物和其应用 |
| CN103911445B (zh) * | 2014-03-20 | 2015-08-12 | 上海科亦生物科技有限公司 | 一种as-pcr引物设计方法、基因多态性检测方法及试剂盒 |
| CN114272966B (zh) | 2015-07-22 | 2023-11-07 | 北卡罗来纳-查佩尔山大学 | 含有具有空间分离的珠保留和信号检测段的珠孔几何结构的流体装置和相关方法 |
| JP7221199B2 (ja) * | 2016-09-15 | 2023-02-20 | エフ． ホフマン－ラ ロシュ エージー． | マルチプレックスｐｃｒの実施方法 |
| US12234377B2 (en) | 2018-05-18 | 2025-02-25 | The University Of North Carolina At Chapel Hill | Compositions, devices, and methods for improving a surface property of a substrate |
| WO2023125898A1 (zh) * | 2021-12-31 | 2023-07-06 | 柏锘（上海）医疗科技有限公司 | 核酸检测方法与系统 |
| CN114350774B (zh) * | 2022-01-28 | 2024-07-09 | 赛纳生物科技(北京)有限公司 | 一种芯片表面固相引物剪切效率的检测方法 |
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| FR2650840B1 (fr) | 1989-08-11 | 1991-11-29 | Bertin & Cie | Procede rapide de detection et/ou d'identification d'une seule base sur une sequence d'acide nucleique, et ses applications |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| GB9211979D0 (en) | 1992-06-05 | 1992-07-15 | Buchard Ole | Uses of nucleic acid analogues |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| DE69432919T2 (de) * | 1993-12-28 | 2004-05-27 | Tanabe Seiyaku Co., Ltd. | Verfahren für den Nachweis spezifischer Polynukleotiden |
| ATE318327T1 (de) * | 1996-06-04 | 2006-03-15 | Univ Utah Res Found | Fluoreszenz-donor-akzeptor paar |
| US6248518B1 (en) | 1996-10-29 | 2001-06-19 | Board Of Regents Of University Of Nebraska | Method for detecting point mutations in DNA utilizing fluorescence energy transfer |
| US5849497A (en) * | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| FR2779154B1 (fr) | 1998-05-27 | 2002-07-12 | Bio Merieux | Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre |
| AU3274500A (en) * | 1999-03-18 | 2000-10-09 | Exiqon A/S | Detection of mutations in genes by specific lna primers |
| EP1250452A1 (en) * | 1999-12-02 | 2002-10-23 | DNA Sciences, Inc. | Methods for determining single nucleotide variations and genotyping |
| JP3638516B2 (ja) * | 2000-09-28 | 2005-04-13 | 株式会社日立製作所 | 核酸検出方法および核酸検出キット |
| US6391592B1 (en) * | 2000-12-14 | 2002-05-21 | Affymetrix, Inc. | Blocker-aided target amplification of nucleic acids |
| JP2005504281A (ja) | 2001-09-24 | 2005-02-10 | ワン ラムダ | 診断用プローブ検出システム |
| EP1481088B1 (en) * | 2002-03-01 | 2008-09-17 | Integrated Dna Technologies, Inc. | Polynomial amplification of nucleic acids |
| US20030211483A1 (en) | 2002-05-09 | 2003-11-13 | Schroeder Benjamin G. | Methods for the enrichment of low-abundance polynucleotides |
| WO2004003173A2 (en) * | 2002-07-01 | 2004-01-08 | Cleveland State University | Method for detecting mutated polynucleotides within a large population of wild-type polynucleotides |
| JP4116856B2 (ja) * | 2002-10-02 | 2008-07-09 | 富士フイルム株式会社 | １塩基多型の検出方法 |
| US20040121374A1 (en) | 2002-10-02 | 2004-06-24 | Yoshihide Iwaki | Method for detecting single nucleotide polymorphisms |
| GB2395556A (en) | 2002-11-22 | 2004-05-26 | Dynal Biotech Ltd | Nucleic acid probe |
| WO2004065628A1 (en) * | 2003-01-21 | 2004-08-05 | Guoliang Fu | Quantitative multiplex detection of nucleic acids |
| US20060183136A1 (en) * | 2004-11-05 | 2006-08-17 | Genevieve Pont-Kingdon | Method for haplotyping and genotyping by melting curve analysis of hybridization probes |
| WO2007008997A2 (en) | 2005-07-12 | 2007-01-18 | The Board Of Trustees Of The Leland Stanford Junior University | Template specific inhibition of pcr |
| WO2007045890A1 (en) | 2005-10-21 | 2007-04-26 | Primerdesign Ltd | Probe |
| US20100009355A1 (en) | 2006-03-14 | 2010-01-14 | Kolodney Michael S | Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides |
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2008
- 2008-02-29 WO PCT/GB2008/000699 patent/WO2008104791A1/en not_active Ceased
- 2008-02-29 EP EP08750469.2A patent/EP2129802B1/en not_active Not-in-force
- 2008-02-29 JP JP2009551268A patent/JP5235905B2/ja not_active Expired - Fee Related
- 2008-02-29 US US12/529,203 patent/US8440405B2/en not_active Expired - Fee Related
- 2008-02-29 CN CN200880012889.8A patent/CN101680030B/zh not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO2008104791A1 (en) | 2008-09-04 |
| EP2129802B1 (en) | 2016-04-13 |
| CN101680030A (zh) | 2010-03-24 |
| JP2010519896A (ja) | 2010-06-10 |
| US8440405B2 (en) | 2013-05-14 |
| GB0703997D0 (en) | 2007-04-11 |
| CN101680030B (zh) | 2014-05-21 |
| US20100184036A1 (en) | 2010-07-22 |
| EP2129802A1 (en) | 2009-12-09 |
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