JP5185695B2 - レーザ顕微鏡装置及び標本画像取得方法 - Google Patents

レーザ顕微鏡装置及び標本画像取得方法 Download PDF

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JP5185695B2
JP5185695B2 JP2008135523A JP2008135523A JP5185695B2 JP 5185695 B2 JP5185695 B2 JP 5185695B2 JP 2008135523 A JP2008135523 A JP 2008135523A JP 2008135523 A JP2008135523 A JP 2008135523A JP 5185695 B2 JP5185695 B2 JP 5185695B2
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frequency
pulsed laser
laser light
optical paths
pulse
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Japanese (ja)
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JP2009281923A5 (enExample
JP2009281923A (ja
Inventor
真一 瀧本
武 橋本
博一 久保
竜男 中田
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Olympus Corp
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Olympus Corp
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Priority to JP2008135523A priority Critical patent/JP5185695B2/ja
Priority to EP09006686A priority patent/EP2124040A1/en
Priority to US12/469,007 priority patent/US8159663B2/en
Publication of JP2009281923A publication Critical patent/JP2009281923A/ja
Publication of JP2009281923A5 publication Critical patent/JP2009281923A5/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N2021/653—Coherent methods [CARS]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N2021/653—Coherent methods [CARS]
    • G01N2021/656—Raman microprobe

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  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
JP2008135523A 2008-05-23 2008-05-23 レーザ顕微鏡装置及び標本画像取得方法 Active JP5185695B2 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2008135523A JP5185695B2 (ja) 2008-05-23 2008-05-23 レーザ顕微鏡装置及び標本画像取得方法
EP09006686A EP2124040A1 (en) 2008-05-23 2009-05-18 Laser microscope apparatus
US12/469,007 US8159663B2 (en) 2008-05-23 2009-05-20 Laser microscope apparatus having a frequency dispersion adjuster

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2008135523A JP5185695B2 (ja) 2008-05-23 2008-05-23 レーザ顕微鏡装置及び標本画像取得方法

Publications (3)

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JP2009281923A JP2009281923A (ja) 2009-12-03
JP2009281923A5 JP2009281923A5 (enExample) 2011-06-23
JP5185695B2 true JP5185695B2 (ja) 2013-04-17

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JP2008135523A Active JP5185695B2 (ja) 2008-05-23 2008-05-23 レーザ顕微鏡装置及び標本画像取得方法

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Country Link
US (1) US8159663B2 (enExample)
EP (1) EP2124040A1 (enExample)
JP (1) JP5185695B2 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009029831A1 (de) 2009-06-17 2011-01-13 W.O.M. World Of Medicine Ag Vorrichtung und Verfahren für die Mehr-Photonen-Fluoreszenzmikroskopie zur Gewinnung von Informationen aus biologischem Gewebe
JP5649828B2 (ja) * 2010-02-03 2015-01-07 オリンパス株式会社 レーザ顕微鏡装置
WO2011099938A1 (en) * 2010-02-12 2011-08-18 National University Of Singapore Method for supplying light beams for integrated cars and multiphoton microscopy
JP5508901B2 (ja) * 2010-03-03 2014-06-04 オリンパス株式会社 レーザ顕微鏡装置の作動方法
DE102010015963A1 (de) * 2010-03-15 2011-09-15 Leica Microsystems Cms Gmbh Vorrichtung und Verfahren zur simultanen Fluoreszenzanregung (2-Wellenlängen-IR)
JP5551477B2 (ja) * 2010-03-15 2014-07-16 オリンパス株式会社 光源装置およびレーザ走査型顕微鏡装置
JP5489804B2 (ja) * 2010-03-23 2014-05-14 オリンパス株式会社 Ｃａｒｓ光用ユニットおよび顕微鏡システム
JP2011200367A (ja) * 2010-03-25 2011-10-13 Fujifilm Corp 画像撮像方法および装置
DE102011005432A1 (de) 2011-03-11 2012-09-13 Hellma Gmbh & Co. Kg Vorrichtung für die Analyse einer kleinen Flüssigkeitsmenge
JP2012211977A (ja) * 2011-03-31 2012-11-01 Nisca Corp 光量調整装置及びこれを備えた光学機器
US9356413B2 (en) * 2011-09-30 2016-05-31 Olympus Corporation Laser source apparatus and laser microscope
CN104237199A (zh) * 2014-08-28 2014-12-24 中国农业大学 一种基于扩散方式的拉曼检测系统和方法
DE102016109303A1 (de) 2016-05-20 2017-11-23 Friedrich-Schiller-Universität Jena Lasermikroskop mit Ablationsfunktion
US20230110264A1 (en) * 2021-09-09 2023-04-13 The Regents Of The University Of California Light-driven ultrafast electric gating
KR102753085B1 (ko) * 2021-12-27 2025-01-10 고려대학교 산학협력단 광대역 비표지 분자 이미징 시스템
US20240118063A1 (en) * 2022-10-07 2024-04-11 Feradyne Outdoors, Llc Lighted arrow

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6108081A (en) * 1998-07-20 2000-08-22 Battelle Memorial Institute Nonlinear vibrational microscopy
JP2002107301A (ja) * 2000-10-03 2002-04-10 Univ Osaka コヒーレントアンチストークスラマン散乱顕微鏡
JP4845279B2 (ja) 2001-02-09 2011-12-28 オリンパス株式会社 生体機能測定方法
JP4854878B2 (ja) * 2001-07-03 2012-01-18 オリンパス株式会社 レーザー顕微鏡
US7623908B2 (en) * 2003-01-24 2009-11-24 The Board Of Trustees Of The University Of Illinois Nonlinear interferometric vibrational imaging
US7586618B2 (en) * 2005-02-28 2009-09-08 The Board Of Trustees Of The University Of Illinois Distinguishing non-resonant four-wave-mixing noise in coherent stokes and anti-stokes Raman scattering
JP2009150649A (ja) * 2005-03-29 2009-07-09 Osaka Univ 空間情報検出装置
JP4869734B2 (ja) * 2005-04-25 2012-02-08 オリンパス株式会社 多光子励起走査型レーザ顕微鏡
US7525724B2 (en) * 2006-03-16 2009-04-28 The University Of Kansas Laser system for photonic excitation investigation
US7542137B2 (en) * 2006-07-24 2009-06-02 University Of Ottawa Pathogen detection using coherent anti-stokes Raman scattering microscopy
JP2008076361A (ja) * 2006-09-25 2008-04-03 Toshiba Corp 顕微鏡装置及び分析装置
CN101617407A (zh) 2006-10-27 2009-12-30 阿里戴斯公司 使用量子系统识别和量子控制技术用于医疗诊断和治疗目的
JP4992083B2 (ja) * 2006-11-07 2012-08-08 国立大学法人大阪大学 屈折率測定方法および屈折率測定装置、光学部材の製造方法

Also Published As

Publication number Publication date
US20090290150A1 (en) 2009-11-26
US8159663B2 (en) 2012-04-17
EP2124040A1 (en) 2009-11-25
JP2009281923A (ja) 2009-12-03

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