JP5090074B2 - Improvement of feed efficiency and reduction of fish meal - Google Patents

Improvement of feed efficiency and reduction of fish meal Download PDF

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JP5090074B2
JP5090074B2 JP2007157302A JP2007157302A JP5090074B2 JP 5090074 B2 JP5090074 B2 JP 5090074B2 JP 2007157302 A JP2007157302 A JP 2007157302A JP 2007157302 A JP2007157302 A JP 2007157302A JP 5090074 B2 JP5090074 B2 JP 5090074B2
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fish meal
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亮介 水谷
拓実 大川
聡 比留間
忠 小林
三津幸 堀内
康久 高橋
善敬 林
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Nosan Corp
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

Description

本発明は水産飼料の経済性を顕著に高める技術に関し、より詳細にはタケ亜科植物成分を含有する飼料を養殖魚介類に投与することによって、飼料の効率を向上させ、成長を顕著に促進し、さらに飼料中の魚粉配合率削減を可能とすることができる水産飼料の改善技術に関する。   The present invention relates to a technology for significantly improving the economics of aquatic feeds, and more particularly, by administering a feed containing a bamboo subfamily plant component to cultured seafood, feed efficiency is improved and growth is significantly promoted. Further, the present invention relates to a technique for improving aquatic feed that can reduce the mixing ratio of fish meal in the feed.

養殖対象となる水産動物は、陸上動物に較べ蛋白要求率が高く、したがって水産養殖用飼料の多くは高い蛋白質含量となっている。   Aquaculture animals to be cultivated have a higher protein requirement rate than terrestrial animals. Therefore, most aquaculture feeds have a high protein content.

水産飼料の原料は、高い蛋白質含量のものが求められるが、中でも動物性の蛋白源として魚粉は重要であり、水産飼料の主原料となっている。   The raw material for aquatic feeds is required to have a high protein content. Above all, fish meal is important as an animal protein source and has become the main raw material for aquatic feeds.

魚粉は、近海で大量に漁獲されたマイワシ等から生産されていたが、近年その漁獲量が激減したため、国内生産量も激減しており、その供給をペルーやチリなどの南米からの輸入に依存せざるを得ない状況となっている。   Fish meal was produced from sardines caught in large quantities in the near sea, but since the catch has drastically decreased in recent years, domestic production has also decreased sharply, and its supply depends on imports from South America such as Peru and Chile. The situation is inevitable.

世界的に見たイワシ等の多獲魚の資源量は、エルニーニョなどの気候条件にも左右され、魚粉の供給を担う南米諸国においては、その資源維持のための漁獲制限が行われており、魚粉の供給不安を招いている。   The global stock of sardines and other high-yielding fish is influenced by climatic conditions such as El Nino, and in South American countries that supply fish meal, there are restrictions on fishing to maintain that resource. Is insecure.

一方において、近年北欧・中国・東南アジア・北米等の諸外国において魚介類の養殖がさかんとなり、これに伴い養殖用配合飼料の主原料である魚粉が世界的に逼迫し、価格の高騰を見せている。特に、中国での需要が多く、今後もさらにその増加が予想される。   On the other hand, in recent years, the cultivation of seafood has become increasingly popular in other countries such as Northern Europe, China, Southeast Asia, and North America. As a result, fish meal, which is the main ingredient of the mixed feed for aquaculture, has been tightened worldwide, and prices have risen. Yes. In particular, there is much demand in China, and further increase is expected in the future.

植物性の高蛋白原料としては、比較的安価な大豆油粕やコーングルテンミールなどがあるが、多くの水産動物ではその利用率が低く、有効に利用されていない。   Plant-based high protein raw materials include soybean oil cake and corn gluten meal, which are relatively inexpensive, but their utilization rate is low in many marine animals and they are not used effectively.

また、これら植物性飼料原料も気候条件によってその収穫量の変動が大きく、さらに石油に代わる環境に優しいバイオエタノール原料としての需要拡大が重なって、その供給不安とともに価格上昇の傾向にある。   In addition, the yield of these plant-based feedstocks varies greatly depending on the climatic conditions. Furthermore, the growing demand for environmentally-friendly bioethanol alternatives to oil is increasing, and there is a tendency to increase prices along with supply concerns.

わが国の水産養殖業は、長引く不況の中での魚価の低迷と消費の減退により疲弊しており、生産コスト引き下げのための経済性の高い飼料が求められている。   Japan's aquaculture industry is exhausted due to sluggish fish prices and declining consumption in a prolonged recession, and there is a need for economical feed to reduce production costs.

これまで、多くの研究者によって、魚粉を削減し比較的安価な植物性原料を使用した飼料の開発が行われてきたが、多くの水産動物においては植物性原料の利用率が低いため、飼料効率の低下を招いたり、魚粉に多く含まれるタウリンの不足により緑肝症が発生する等、解決すべき問題が山積しており、理想的な魚粉削減飼料は完成していない。   Until now, many researchers have developed feeds that use less expensive plant materials and reduce fish meal. However, in many aquatic animals, the feed rate of plant materials is low. There are a lot of problems to be solved, such as a decrease in efficiency and the occurrence of green liver disease due to a lack of taurine contained in a large amount of fish meal, and an ideal fish meal-reducing feed has not been completed.

タケ亜科植物は貴重なバイオマス素材であり、様々な薬理効果等が報告されているが、北海道や東北地方などの山間部に自生するチシマザサやクマイザサを中心に膨大な資源量があるが、その資源の大半は未利用で放置されている状況にある。   Bamboo subfamily is a valuable biomass material, and various pharmacological effects have been reported, but there is a huge amount of resources mainly in Chishimasa and Kumaisasa that grow naturally in mountainous areas such as Hokkaido and Tohoku. Most of the resources are unused and left unattended.

タケ亜科植物の飼料への応用技術に関しては、「熊笹エキス飼料の製造方法」(特公昭61−28350号公報(特許文献1))があるが、これは馬の好物である熊笹の代わりに熊笹のエキスを飼料として与えるもので、熊笹の代替飼料に関するものである。また、「竹又は笹の抽出成分を含んでなる飼料用添加剤」(特開2005−151928号公報(特許文献2))は、ペット等の罹病率の低下等を意図したものである。   Regarding the application technology of bamboo subfamily plants to the feed, there is a “method for producing a bear extract extract” (Japanese Patent Publication No. 61-28350 (Patent Document 1)). It is an extract that gives an extract of Kumagusu as a feed, and relates to an alternative feed for Kumagusu. Moreover, “additive for feed comprising bamboo or persimmon extract” (Japanese Patent Laid-Open No. 2005-151928 (Patent Document 2)) is intended to reduce the morbidity of pets and the like.

すなわち、本願発明のような水産飼料における飼料効率の改善や魚粉削減に関係したものではない。   That is, it is not related to improvement of feed efficiency or fish meal reduction in aquatic feed as in the present invention.

先にも述べたとおり、養殖業者は生産コスト引き下げのために、より経済性の高い飼料を求めている。この要望に答えるために、需給が逼迫し高騰している魚粉の配合率を削減し、より安価な植物性原料を多用してもなお飼料効率が低下しない飼料の開発が待たれているところである。   As mentioned earlier, farmers are looking for more economical feed to reduce production costs. In order to answer this demand, we are waiting for the development of feed that will reduce the mixing ratio of fish meal, which is in tight supply and demand, and will not decrease feed efficiency even if more cheap plant materials are used. .

特公昭61−28350号公報Japanese Patent Publication No. 61-28350 特開2005−151928号公報JP 2005-151928 A

原料となるイワシ等の漁獲量の変動や世界的に旺盛な需要により、価格上昇圧力が高まっている魚粉や魚油は、現在の水産飼料における主原料である。また、気候条件によって収穫量の変動が大きいトウモロコシや大豆等の植物性飼料原料も、石油に代わる環境に優しいバイオエタノール原料としての需要拡大が重なって、その供給不安とともに価格上昇の傾向にある。水産飼料に使用されるこれらの貴重な資源を最大限に有効利用し、且つ養殖分野において未開発の安価なバイオマス素材を発掘し、それを飼料に応用することによって、餌飼料経費の上昇を抑え養殖業者の経営安定に資する技術を完成させることを課題とする。   Fish meal and fish oil, which are under increasing price pressure due to fluctuations in the catch of sardines and other raw materials, as well as global demand, are the main raw materials in current aquatic feed. In addition, plant feed materials such as corn and soybean, whose yields vary greatly depending on the climatic conditions, are increasing in demand as environmentally friendly bioethanol raw materials instead of petroleum. By making the best use of these precious resources used in aquatic feeds, and by discovering undeveloped inexpensive biomass materials in the aquaculture field and applying them to feed, the increase in feed costs is suppressed. The goal is to complete technology that contributes to the stability of farmer management.

本発明者らは、各種の素材を用い、養殖魚介類への投与効果について鋭意研究を積み重ねた結果、様々な機能が謳われているタケ亜科植物成分が、水産飼料の効率を著しく改善する効果を有することを見出した。また、さらにタケ亜科植物成分が、飼料中の魚粉配合率を削減して安価な植物性飼料原料を利用するために有効であることを見出し、本発明を完成させた。   As a result of intensive studies on the effects of administration on cultured fish and shellfish using various materials, the present inventors have found that the bamboo subfamily plant component, which has various functions, significantly improves the efficiency of aquatic feed. It was found to have an effect. Furthermore, the present inventors have found that a bamboo subfamily plant component is effective for reducing the proportion of fish meal in the feed and using inexpensive plant feed raw materials, thereby completing the present invention.

すなわち、本発明は
(1)タケ亜科植物成分を含有してなる飼料効率改善用及び/又は魚粉配合率削減用水産飼料、
(2)タケ亜科植物成分が、タケ亜科植物の葉及び茎を原料とし、その全乾燥物、抽出物及び抽出残渣あるいはその乾燥物の少なくとも1種以上を含有することを特徴とする(1)記載の水産飼料、
(3)水産飼料が鮭鱒類養殖用及び/又はアユ養殖用であることを特徴とする(1)又は(2)記載の飼料、
(4)乾燥飼料中のタケ亜科植物成分の含有量が、全乾燥物では0.01重量%〜1重量%、抽出物では0.005〜0.1重量%、抽出残渣の乾燥物では0.05〜2重量%であることを特徴とする(2)又は(3)記載の飼料
に関する。
That is, the present invention
(1) Aquatic feed for improving feed efficiency and / or reducing fish meal content, comprising bamboo subfamily plant components,
(2) The bamboo subfamily plant component is made from the leaves and stems of the bamboo family plant, and contains at least one or more of the dried product, extract and extract residue or dried product thereof ( 1) Seafood feed as described
(3) The feed according to (1) or (2), wherein the aquatic feed is for cultivating moss and / or ayu
(4) The content of the Bamboo subfamily plant component in the dry feed is 0.01% to 1% by weight in the total dry product, 0.005 to 0.1% by weight in the extract, and in the dry product of the extraction residue The feed according to (2) or (3), which is 0.05 to 2% by weight.

本発明のタケ亜科植物には、全てのタケ・ササ類を包含するが、中でもササ類ササ属植物の応用が望ましく、ミヤコザサ、チマキザサ、チシマザサ、クマイザサ、クマザサ等のササが挙げられるが、資源量の豊富さと収穫効率の良さなどの理由から、特にチシマザサとクマイザサの活用が望ましい。   The bamboo subfamily of the present invention includes all bamboo and Sasa species. Among them, the application of Sasa genus Sasa is desirable, and Sasa such as Miyakosasa, Chimakisa, Chishimasa, Kumizasa, Kumazasa, etc. For reasons such as abundant quantities and good harvesting efficiency, it is particularly desirable to use Chishimazasa and Kumaizasa.

本発明のタケ亜科植物成分を得るための出発材料は採集した葉や茎である。本発明の全乾燥物とは、収穫したタケ亜科植物の葉と茎を細断し、それをパドル型回転乾燥機等で水分が10%以下となるまで乾燥してから、必要に応じてハンマーミルやバーチカルミル、ビンミル等でさらに粉砕して得られたものである。   The starting material for obtaining the bamboo subfamily component of the present invention is the collected leaves and stems. The total dry matter of the present invention is to chop the leaves and stems of the harvested bamboo subfamily plant, dry it with a paddle type rotary dryer or the like until the water content is 10% or less, and if necessary It was obtained by further grinding with a hammer mill, vertical mill, bin mill or the like.

本発明の抽出物は、水や温水又は熱水で抽出する方法、アルコールなどの溶剤で抽出する方法、加圧して搾り出す方法あるいはこれらの方法を組み合わせる方法等様々な製法があり、そのいずれの製法によっても本発明の抽出物を得ることができるが、新鮮な生の材料を高圧・低温で圧搾し、それを減圧濃縮して得られるものが有効成分の変性が極めて少ない点で好適である。抽出物は液体であり、それを必要に応じて適度に希釈して養殖現場でペレットなどに添加して使用することもできるが、これを噴霧乾燥や凍結乾燥などの方法で乾燥粉末としたほうが工業的には使いやすい。   The extract of the present invention has various production methods such as a method of extracting with water, hot water or hot water, a method of extracting with a solvent such as alcohol, a method of pressing and squeezing, or a method of combining these methods. The extract of the present invention can also be obtained by the production method, but the fresh raw material is obtained by pressing under high pressure and low temperature and concentrating it under reduced pressure, which is preferable in that the active ingredient is very little denatured. . The extract is a liquid, and it can be diluted as needed and added to pellets etc. at the farming site, but it is better to use this as a dry powder by spray drying or freeze drying. Easy to use industrially.

抽出残渣とは上記のような方法で抽出物を抽出した残渣のことであり、湿潤品として得られ、そのままでも使用できるが、パドル型回転乾燥機などでこれを乾燥し、さらにハンマーミルやバーチカルミル、ビンミル等によって粉砕し、乾燥粉末品として使用する方法が輸送や保管・使用に便利である。   The extraction residue is a residue obtained by extracting the extract by the method as described above, and it is obtained as a wet product and can be used as it is. However, it is dried with a paddle type rotary dryer, and further, a hammer mill or vertical A method of pulverizing with a mill, a bin mill or the like and using it as a dry powder product is convenient for transportation, storage and use.

本発明は、ブリ、マダイ、カンパチ、ヒラメ、トラフグ、スズキ、マアジ、サバ、ギンザケ、大西洋サケ、ウナギ、コイ、ニジマス、アユ等の養殖魚類、クルマエビ等の養殖甲殻類、アワビ等の貝類、観賞用の魚介類等、餌飼料を給餌して管理飼育されている全ての動物に適用できるが、特に鮭鱒類及びアユにおいてその効果が顕著である。   The present invention includes yellowtail, red sea bream, amberjack, flounder, sea bass, sea bass, mackerel, coho salmon, Atlantic salmon, eel, carp, rainbow trout, cultured fish such as sweetfish, cultured crustacean such as prawn, shellfish such as abalone, ornamental It can be applied to all animals that are fed and managed by feeding, such as fish and shellfish, and the effect is particularly remarkable in moss and sweetfish.

本発明に言う水産飼料とは、(1)ドライペレットのような固形状乾燥飼料、(2)ドライペレットを破砕し篩いで分級したクランブル状乾燥飼料、(3)比較的高水分のペレットであって用時に加水させて給餌される膨化タイプ飼料、(4)給餌高速撹拌造粒装置等の装置を用いて造粒した顆粒状乾燥飼料、(5)原料を単に混合・粉砕した飼料であってそれに水を加えて練り上げて給餌される粉末状乾燥飼料、(6)生餌と粉末状乾燥飼料を混合・破砕・成形して給餌されるモイストペレット、そのモイストペレット製造時に混ぜ込んで使用するペレットタイプの固形状乾燥飼料、(7)粉末状飼料のみに水を加えて成形後使用されるシングルモイストペレット、(8)種苗生産において培養され稚仔に給餌されるワムシやアルテミア等用の餌飼料、(9)生原料を多用するペースト状飼料、(10)生餌として単独使用されるイワシ・アジ等の小型の漁獲魚、及び(11)ドライペレットやクランブル、高水分膨化ペレット飼料、モイストペレット、練りや加水用の水、ペースト状飼料等に適宜添加して使用される飼料添加剤など、全ての餌飼料を指す。   The aquatic feed according to the present invention is (1) a solid dry feed such as a dry pellet, (2) a crumbled dry feed obtained by crushing and classifying the dry pellet with a sieve, and (3) a relatively high moisture pellet. (4) Granulated dry feed granulated using a device such as a feeding high-speed agitation granulator, (5) Feed simply mixed and crushed raw materials Powdered dry feed that is fed by adding water and kneaded to it, (6) Moist pellets that are fed by mixing, crushing, and shaping raw feed and powdered dry feed, and pellet types that are mixed and used when producing the moist pellets (7) Single moist pellets that are used after molding by adding water to powdered feed only, (8) Rotifers and artemia that are cultured in seedling production and fed to juveniles (9) Paste feed that uses a lot of raw materials, (10) Small catch fish such as sardines and horse mackerel used alone as raw feed, and (11) Dry pellets and crumbles, high moisture expanded pellet feed It refers to all feeds such as moist pellets, water for kneading and hydration, and feed additives used by adding to paste-like feeds.

これらのうち、(1)、(2)及び(4)などのような乾燥飼料に対する本発明のタケ亜科植物全乾燥物の添加率は、0.01重量%〜1重量%、抽出物の添加率は、0.005重量%〜0.1重量%、抽出残渣の乾燥物の添加率は、0.05重量%〜2重量%であり、この添加率が基本である。さらに望ましくは、全乾燥物では0.05重量%前後、抽出物では0.01重量%前後、抽出残渣の乾燥物では0.5重量%〜1重量%の範囲である。   Among these, the addition ratio of the dried bamboo shoots of the present invention to the dried feed such as (1), (2) and (4) is 0.01% to 1% by weight of the extract. The addition rate is 0.005 wt% to 0.1 wt%, and the dry residue addition rate is 0.05 wt% to 2 wt%. This addition rate is the basis. More desirably, the total dry weight is about 0.05% by weight, the extract is about 0.01% by weight, and the dry residue of the extraction residue is in the range of 0.5% to 1% by weight.

各素材ともに前記の下限値を下回る添加率では、飼料効率の改善効果が判然とせず、上限値を上回る添加率では、餌飼料自体の粗蛋白等の栄養値が下がることもあって成長が制限される恐れがあることと費用対効果の面でマイナスとなりうるからである。なお、本発明に言う乾燥物とは、通常は水分が5〜10%程度のものを指す。   If the addition rate is lower than the above lower limit for each material, the effect of improving feed efficiency is not obvious, and if the addition rate exceeds the upper limit, the nutrient value such as crude protein in the feed itself may decrease, limiting growth. This is because it can be negative and cost-effective. In addition, the dry substance said to this invention points out a thing with a water | moisture content of about 5 to 10% normally.

この乾燥飼料への基本添加率とその標準的な給餌率に基づけば、魚介類の単位体重あたりの各素材の投与量が算定される。さらにこの数字に基づいて、給餌率が様々である上記(3)、(5)〜(10)のような餌飼料への添加率を適宜計算すればよい。また、(11)の飼料添加剤では、上述の乾燥飼料への基本添加率と飼料添加剤の餌飼料に対する添加率などから、飼料添加剤中の各素材の配合率が決定される。   Based on the basic rate of addition to this dry feed and its standard feeding rate, the dose of each ingredient per unit weight of seafood is calculated. Furthermore, based on these numbers, the rate of addition to the feed such as the above (3), (5) to (10) with various feeding rates may be calculated as appropriate. In addition, in the feed additive of (11), the blending ratio of each material in the feed additive is determined from the basic addition rate to the dry feed and the addition rate of the feed additive to the feed feed.

本発明のタケ亜科植物成分の投与効果は、投与を開始して2〜3週間で早くも現れはじめるが、飼育の全期間にわたって投与することにより、顕著な飼料効率の改善効果を奏する。   The administration effect of the bamboo subfamily plant component of the present invention begins to appear as early as 2 to 3 weeks after the start of the administration, but when administered over the entire period of breeding, there is a remarkable effect of improving feed efficiency.

本発明により、飼料効率が改善され、他の高価な生餌用小型魚類、魚粉や魚油、大豆あるいはトウモロコシや小麦等の有用資源を有効活用することができるとともに、現在、その多くが活用されていないタケ亜科植物のバイオマス資源を水産養殖において有効に利用することができる。   According to the present invention, feed efficiency is improved, and useful resources such as other expensive small fish for live food, fish meal and fish oil, soybean, corn and wheat can be effectively utilized, and many of them are currently utilized. The biomass resources of no bamboo subfamily can be effectively used in aquaculture.

以下に実施例で本発明を具体的に説明するが、本発明はこれらの実施例に何ら限定されるものではない。   EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples.

(ニジマスでの水槽試験)
タケ亜科植物成分として使用したチシマザサの抽出残渣乾燥粉末は、(株)ヒロインターナショナルから入手したものを使用した。この粉末は、収穫したチシマザサの葉と茎を細断して高圧・低温で搾汁し、樹液を採取した残りの残渣をパドル型回転乾燥機で乾燥してから、ビンミルで粉砕して得られたものである。
(Water tank test in rainbow trout)
The dry residue powder of Chishimazasa used as a bamboo subfamily component was obtained from Hiro International Co., Ltd. This powder is obtained by shredding the harvested leaves and stems, squeezing them at high pressure and low temperature, drying the remaining sap from the paddle-type rotary dryer, and then crushing it with a bin mill. It is a thing.

Figure 0005090074
Figure 0005090074

表1に示す3種類(飼料1、飼料2及び飼料3)の配合内容の試験飼料を作製した。
魚油を除く各原料を秤量・混合したのち、JIS標準篩32メッシュ以下に粉砕し、飼料2と飼料3については魚油を添加、これに約4%の水を加えて混練したのち、ディスクペレッターを用いて直径約3.2mmのペレット状に成型し、これを熱風乾燥機を用いて乾燥してハードペレット飼料を作製した。飼料1は対照飼料であり、飼料2は魚粉の配合割合を減じて大豆油粕の配合割合を増加した飼料、飼料3は飼料2にエキス抽出残渣乾燥粉末を0.1重量%配合した飼料である。
Test feeds having the blending contents of three types (feed 1, feed 2 and feed 3) shown in Table 1 were prepared.
After weighing and mixing each raw material except fish oil, pulverize to JIS standard sieve 32 mesh or less, add fish oil to feed 2 and feed 3, add about 4% water to this, knead, and then disk pelleter Was molded into a pellet shape having a diameter of about 3.2 mm, and dried using a hot air dryer to prepare a hard pellet feed. Feed 1 is a control feed, feed 2 is a feed in which the blending ratio of fish meal is reduced and the blending ratio of soybean oil cake is increased, and feed 3 is a feed in which 0.1% by weight of extract extract residue dry powder is blended with feed 2. .

平均体重が45g前後のニジマスを供試し、各試験飼料について、300リットル容のパンライト製水槽を2水槽ずつ用いて、各水槽に総体重を測定した15尾ずつを収容し、エアレーションを施して水温16〜17℃の流水で飼育した。各飼料は朝昼夕の3回に分け、原則として日曜日を除く毎日、飽食給餌を行って摂餌量を記録した。試験期間は52日間で給餌日数は40日であった。試験終了日に各水槽内の供試魚の総体重測定と尾数確認を行った。   A rainbow trout with an average weight of around 45 g was tested, and for each test feed, two 300-liter panlite water tanks were used, each of which contained 15 fish whose total body weight was measured, and aerated. The animals were bred under running water at a water temperature of 16-17 ° C. Each feed was divided into three times of morning and evening, and in principle, every day except Sunday, a satiety feeding was performed and the amount of food intake was recorded. The test period was 52 days and the number of feeding days was 40 days. The total body weight of the test fish in each tank and the number of fish were confirmed on the test end date.

Figure 0005090074
Figure 0005090074

飼育試験の結果は表2のとおりとなった。生残率はいずれの水槽でも100%であった。   The results of the breeding test are shown in Table 2. The survival rate was 100% in any water tank.

飼料効率の算出は、次式に拠った。
(終了時総体重−開始時総体重)÷給餌量×100=飼料効率
The calculation of feed efficiency was based on the following formula.
(Total weight at end-total weight at start) / feeding amount × 100 = feed efficiency

また、個体平均増重倍率は、次式に拠った。
終了時平均体重÷開始時平均体重×100=個体平均増重倍率
Moreover, the individual average weight multiplication factor was based on the following formula.
Average weight at the end / Average weight at the start × 100 = Individual average weight gain

対照の飼料1と比較して、飼料効率及び個体平均増重倍率において、飼料2はいずれも劣り、飼料3ではいずれも優れる結果となった。すなわち、魚粉を減らし魚油を入れただけの飼料2は結果が悪く、魚粉を減らし魚油を入れた他に、チシマザサの抽出残渣乾燥粉末を入れた飼料3では良い結果が得られた。この結果から、チシマザサの抽出残渣乾燥粉末の配合により飼料中の魚粉配合率を削減しても飼育成績が落ちることはなく、むしろ向上させることが明らかとなった。   Compared with the control feed 1, the feed 2 was inferior and the feed 3 was superior in terms of feed efficiency and individual average weight gain. That is, the feed 2 in which the fish meal was reduced and the fish oil was added gave poor results, and in addition to the fish meal reduced and the fish oil added, the feed 3 containing the dried extract powder of chishimasa was obtained. From this result, it became clear that even if the fish meal content in the feed was reduced by blending the extract residue dry powder of Chishimasa, the breeding performance would not be reduced, but rather improved.

(アユでの水槽試験)
タケ亜科植物成分として使用したチシマザサの抽出残渣乾燥粉末は、実施例1で使用したものと同様、(株)ヒロインターナショナルから入手したものを使用した。
(Water tank test at Ayu)
Similar to the one used in Example 1, the powder obtained from Hiro International Co., Ltd. was used as the dry residue dry powder of Chishimazasa used as a bamboo subfamily plant component.

Figure 0005090074
Figure 0005090074

表3に示す7種類(飼料4〜飼料10)の配合内容のクランブルタイプの試験飼料を作製した。   A crumble type test feed having the blending contents of 7 types (feed 4 to feed 10) shown in Table 3 was prepared.

各原料を秤量・混合したのち、粉砕機でJIS標準篩32メッシュ以下に粉砕し、コンディショナーで調湿・混練後、2軸エクストルーダーにて直径約6mmのペレット状に成型し、これを熱風乾燥機で乾燥してEP飼料を作製した。このペレットをクランブラーで破砕し、JIS標準篩の12メッシュから24メッシュの間の分画を採取して試験飼料とした。飼料4は対照飼料であり、飼料5〜飼料10はエキス抽出残渣乾燥粉末を各々0.05重量%、0.1重量%、0.5重量%、1重量%、2重量%、5重量%配合し、合計が100となるよう魚粉で調整した飼料である。   Each raw material is weighed and mixed, then pulverized to a JIS standard sieve of 32 mesh or less with a pulverizer, humidity-conditioned and kneaded with a conditioner, molded into a pellet with a diameter of about 6 mm with a twin screw extruder, and dried with hot air EP feed was prepared by drying with a machine. The pellets were crushed with a cumbler, and a fraction between 12 mesh and 24 mesh of a JIS standard sieve was collected and used as a test feed. Feed 4 is a control feed, and feed 5 to feed 10 are 0.05% by weight, 0.1% by weight, 0.5% by weight, 1% by weight, 2% by weight, and 5% by weight, respectively, of the extract extraction residue. It is the feed which mix | blended and adjusted with the fish meal so that the total might be set to 100.

底面積4.8m2、水深1mのコンクリート製8角水槽7水槽を用いて、各水槽に平均体重が約7.2gのアユを総体重として8.46kg(推定尾数は1169尾)ずつ収容し、エアレーションを施して水温16〜17℃の流水で飼育した。各飼料は朝昼夕の3回に分け、原則として日曜日を除く毎日、飽食給餌を行って摂餌量と斃死尾数を記録した。試験期間は30日間で給餌日数は25日であった。試験終了日に各水槽内の供試魚の総体重測定を行った。 Using a concrete octagonal aquarium 7 tanks with a bottom area of 4.8 m 2 and a depth of 1 m, each tank contains 8.46 kg of sweetfish with an average weight of about 7.2 g (estimated number is 1169). Aeration was applied, and the animals were bred under running water at a water temperature of 16 to 17 ° C. Each feed was divided into three times of morning, noon, and evening. As a general rule, every day except Sunday, a satiety feeding was performed, and the amount of food consumed and the number of moribund tails were recorded. The test period was 30 days and the number of feeding days was 25 days. The total body weight of the test fish in each water tank was measured on the test end day.

Figure 0005090074
Figure 0005090074

試験の結果は表4のとおりであった。
飼料効率の算出は、(終了時総体重−開始時総体重)÷給餌量×100で求められるが、飼料6の給餌区については、若干の斃死があり生残率が100%でなかったため、開始時と終了時の総体重、斃死尾数、総給餌量から、補正飼料効率及び生残率を求めた。
The test results are shown in Table 4.
The calculation of the feed efficiency is calculated by (total body weight at the end−total weight at the start) ÷ feeding amount × 100, but for the feeding section of the feed 6, there was some drowning and the survival rate was not 100%. The corrected feed efficiency and survival rate were determined from the total body weight at the start and end, the number of moribund tails, and the total feed amount.

補正飼料効率の算出は、次式に拠った。
〔終了時総体重−開始時総体重+(開始時平均体重+終了時平均体重)÷2×斃死尾数〕÷給餌量×100=補正飼料効率
The calculation of the corrected feed efficiency was based on the following formula.
[Total weight at the end-Total weight at the start + (Average weight at the start + Average weight at the end) ÷ 2 x Number of moribund tails] ÷ Feeding amount x 100 = Corrected feed efficiency

対照の飼料4に較べて、飼料5〜9は、飼料効率と個体平均増重倍率のいずれにおいても優れ、特に抽出残渣乾燥粉末を0.5重量%ないし1重量%配合した飼料7と飼料8が最も優れていたが、同乾燥粉末を5重量%配合した飼料10では劣った。すなわち、チシマザサの抽出残渣乾燥粉末が2重量%までは飼料効率と個体平均増重倍率が対照より優れていたが、チシマザサの抽出残渣乾燥粉末が5重量%になると飼料効率と個体平均増重倍率が対照より劣ってくることが分かった。   Compared to the control feed 4, feeds 5 to 9 are excellent in both feed efficiency and individual average weight gain, especially feed 7 and feed 8 containing 0.5% to 1% by weight of dry powder of extraction residue. However, the feed 10 containing 5% by weight of the dry powder was inferior. In other words, the feed efficiency and the individual average weight gain were better than the control up to 2% by weight of the dry residue extract of Chishimasa, but the feed efficiency and the average gain of the individual weight were 5% by weight of the dry residue extract of Chishimasa. Was found to be inferior to the control.

(ニジマスでの水槽試験)
タケ亜科植物成分として使用したチシマザサの全乾燥物、抽出物、抽出残渣乾燥粉末は、すべて(株)ヒロインターナショナルから入手したものを使用した。全乾燥物は、収穫したチシマザサの葉と茎を細断してそのままパドル型回転乾燥機で乾燥してから、ビンミルで粉砕して得られたものであり、抽出物は、収穫したチシマザサの葉と茎を細断して高圧・低温で搾汁し、それを減圧濃縮した抽出液である。抽出残渣乾燥粉末は、実施例1で使用したものと同様、樹液を採取した残りの残渣を乾燥、粉砕して得られたものである。
(Water tank test in rainbow trout)
All the dried products, extracts, and dry extract powders used as the bamboo subfamily plant components were those obtained from Hiro International Co., Ltd. The whole dried product was obtained by shredding the harvested leaves and stems and drying them directly with a paddle type rotary dryer, and then crushing them with a bin mill. It is an extract obtained by chopping the stem and squeezing it at high pressure and low temperature and concentrating it under reduced pressure. The extraction residue dry powder was obtained by drying and pulverizing the remaining residue from which the sap was collected, similar to that used in Example 1.

Figure 0005090074
Figure 0005090074

表5に示す13種類(飼料11〜飼料23)の配合内容のクランブルタイプの試験飼料を作製した。   A crumble type test feed having a blending content of 13 types (feed 11 to feed 23) shown in Table 5 was prepared.

各原料を秤量・混合したのち、粉砕機でJIS標準篩32メッシュ以下に粉砕し、これに約4%の水を加えて混練したのち、ディスクペレッターを用いて直径約3.2mmのペレット状に成型し、これを熱風乾燥機を用いて乾燥してハードペレット飼料を作製した。このペレットをクランブラーで破砕し、JIS標準篩の10.5メッシュから16メッシュの間の分画を採取して試験に供試した。飼料11は対照飼料であり、飼料12〜飼料17は全乾燥物を各々0.005重量%、0.01重量%、0.05重量%、0.1重量%、0.5重量%、1重量%配合し、飼料18〜飼料22は抽出物を各々0.005重量%、0.01重量%、0.05重量%、0.1重量%、0.5重量%配合し、飼料23はエキス抽出残渣乾燥粉末を0.1重量%配合した飼料である。   Each raw material is weighed and mixed, then pulverized to a JIS standard sieve of 32 mesh or less with a pulverizer, kneaded with about 4% water, and then pelleted in a diameter of about 3.2 mm using a disk pelleter. And dried using a hot air dryer to produce a hard pellet feed. The pellets were crushed with a cumbler, and a fraction between 10.5 mesh and 16 mesh of a JIS standard sieve was collected and used for the test. The feed 11 is a control feed, and the feed 12 to the feed 17 are 0.005% by weight, 0.01% by weight, 0.05% by weight, 0.1% by weight, 0.5% by weight, The feeds 18 to 22 contain 0.005%, 0.01%, 0.05%, 0.1% and 0.5% by weight of the extract, respectively. This is a feed containing 0.1% by weight of dry powder extracted from the extract.

平均体重が約2gのニジマスを供試し、各試験飼料について、縦・横・高さが各々27cm・15cm・17cmのプラスチック製水槽13水槽を用いて、各水槽に総体重を測定した15尾ずつを収容し、エアレーションを施して水温16〜17℃の流水で飼育した。各飼料は朝昼夕の3回に分け、原則として日曜日を除く毎日、飽食給餌を行って摂餌量を記録した。試験期間は17日間で給餌日数は13日であった。試験終了日に各水槽内の供試魚の総体重測定と尾数確認を行った。   A rainbow trout with an average weight of about 2 g was tested, and for each test feed, 15 fishes whose total body weight was measured in each aquarium using 13 aquariums of 27 cm, 15 cm, and 17 cm in height, width, and height, respectively. Were housed, aerated, and reared in running water at a water temperature of 16 to 17 ° C. Each feed was divided into three times of morning and evening, and in principle, every day except Sunday, a satiety feeding was performed and the amount of food intake was recorded. The test period was 17 days and the feeding period was 13 days. The total body weight of the test fish in each tank and the number of fish were confirmed on the test end date.

Figure 0005090074
Figure 0005090074

結果は表6のとおりであった。
生残率はいずれの水槽でも100%であった。対照の飼料11に較べて、飼料13〜飼料16及び飼料18〜飼料21は飼料効率と個体平均増重倍率において優れる結果となった。しかし、抽出物を0.5重量添加した飼料22の飼育成績は対照の飼料11よりも劣った。この結果から、飼料効率改善に最も効果のあるチシマザサ全乾燥物の配合レベルは0.05重量%前後であり、抽出物の配合レベルは0.01重量%前後であることが明らかとなった。また、飼料23の結果から実施例1における抽出残渣乾燥粉末の効果も再確認された。
The results are shown in Table 6.
The survival rate was 100% in any water tank. Compared with the control feed 11, feed 13 to feed 16 and feed 18 to feed 21 were excellent in feed efficiency and average weight gain. However, the breeding performance of the feed 22 to which 0.5 wt% of the extract was added was inferior to the control feed 11. From this result, it became clear that the blending level of the dried twigs most effective for improving feed efficiency is around 0.05% by weight, and the blending level of the extract is around 0.01% by weight. Moreover, the effect of the extraction residue dry powder in Example 1 was confirmed again from the result of the feed 23.

(アユでの水槽試験) (Water tank test at Ayu)

Figure 0005090074
Figure 0005090074

表7に示す6種類(飼料24〜飼料29)の配合内容のクランブルタイプの試験飼料を作製した。   The crumble type test feed of the 6 types (feed 24-feed 29) shown in Table 7 was prepared.

各原料を秤量・混合したのち、粉砕機でJIS標準篩32メッシュ以下に粉砕し、コンディショナーで調湿・混練後、2軸エクストルーダーにて直径約6mmのペレット状に成型し、これを熱風乾燥機で乾燥してEP飼料を作製した。このペレットをクランブラーで破砕し、JIS標準篩の9.2メッシュから14メッシュの間の分画を採取して試験飼料とした。飼料24及び飼料25は対照飼料であり、飼料24は魚粉を約55重量%、飼料25は約50重量%配合している。また、飼料26及び飼料27は魚粉配合率を約50重量%とし、抽出残渣乾燥粉末を各々0.5重量%、1重量%、飼料28及び飼料29は魚粉配合率を約40重量%と大幅に削減し、抽出残渣乾燥粉末を各々0.5重量%、1重量%配合した飼料である。   Each raw material is weighed and mixed, then pulverized to a JIS standard sieve of 32 mesh or less with a pulverizer, humidity-conditioned and kneaded with a conditioner, molded into a pellet with a diameter of about 6 mm with a twin screw extruder, and dried with hot air EP feed was prepared by drying with a machine. This pellet was crushed with a crambler, and a fraction between 9.2 mesh and 14 mesh of JIS standard sieve was collected and used as a test feed. The feed 24 and the feed 25 are control feeds. The feed 24 contains about 55% by weight of fish meal and the feed 25 contains about 50% by weight. In addition, feed 26 and feed 27 have a fish meal blending ratio of about 50% by weight, extraction residue dry powder is 0.5% by weight and 1% by weight, respectively, and feed 28 and feed 29 have a fish meal blending ratio of approximately 40% by weight. The feed was obtained by mixing 0.5% by weight and 1% by weight of the dry powder of the extraction residue, respectively.

底面積4.8m2、水深1mのコンクリート製8角水槽6水槽を用い、各水槽に平均体重約24gのアユを1000尾ずつ収容し、エアレーションを施して水温16〜17℃の流水で飼育した。各飼料は朝昼夕の3回に分け、原則として日曜日を除く毎日、飽食給餌を行って摂餌量を記録した。試験期間は32日間で給餌日数は25日であった。試験終了日に各水槽内の供試魚の総体重測定と尾数確認を行った。 A concrete octagonal aquarium 6 tanks with a bottom area of 4.8 m 2 and a depth of 1 m were used. Each tank contained 1,000 fishes with an average weight of about 24 g, aerated, and bred under running water at a temperature of 16-17 ° C. . Each feed was divided into three times of morning and evening, and in principle, every day except Sunday, a satiety feeding was performed and the amount of food intake was recorded. The test period was 32 days and the number of feeding days was 25 days. The total body weight of the test fish in each tank and the number of fish were confirmed on the test end date.

Figure 0005090074
Figure 0005090074

補正飼料効率の算出は、実施例2と同様に次式に拠った。
〔終了時総体重−開始時総体重+(開始時平均体重+終了時平均体重)÷2×斃死尾数〕÷給餌量×100=補正飼料効率
The calculation of the corrected feed efficiency was based on the following equation as in Example 2.
[Total weight at the end-Total weight at the start + (Average weight at the start + Average weight at the end) ÷ 2 x Number of moribund tails] ÷ Feeding amount x 100 = Corrected feed efficiency

表8に示すとおり、生残率は各区間で変わらなかったが、補正飼料効率では飼料27〜飼料29が、また個体平均増重倍率では飼料28及び飼料29が対照である飼料24よりも優れる結果となった。   As shown in Table 8, the survival rate did not change in each section, but in the corrected feed efficiency, the feed 27 to the feed 29 were superior to the feed 24 in which the feed 28 and the feed 29 were the controls in the individual average weight gain. As a result.

魚粉配合率を約50重量%とした飼料25〜飼料27の比較では、対照である飼料25に較べ、飼料26及び飼料27、即ちチシマザサの抽出残渣乾燥粉末を0.5重量%及び1重量%添加した飼料の給餌区の補正飼料効率、個体平均増重倍率がともに優れる結果となった。   In the comparison of the feed 25 to the feed 27 in which the fish meal mixture ratio was about 50% by weight, the feed residue 26 and the feed 27, that is, the dry powder of the extract of Chichimasasa was 0.5% by weight and 1% by weight, compared with the feed 25 as a control. Both the corrected feed efficiency and the individual average weight gain in the feeding area of the added feed were excellent.

また、魚粉配合率を約40重量%とし、チシマザサの抽出残渣乾燥粉末を0.5重量%及び1重量%添加した飼料28及び飼料29では魚粉配合率を約55重量%とした対照の飼料24、あるいは魚粉配合率を約50重量%とした対照の飼料25よりも補正飼料効率、個体平均増重倍率が優れた結果が得られた。   In addition, in the feed 28 and the feed 29 in which the fish meal mixture ratio was about 40% by weight, and 0.5% by weight and 1% by weight of the dried powdered extract of Chishimasa were added, the control feed 24 in which the fish meal mixture ratio was about 55% by weight. Or, the corrected feed efficiency and the individual average weight gain were superior to the control feed 25 in which the fish meal content was about 50% by weight.

以上の結果から、ニジマスと同様、アユ用飼料においてもチシマザサの抽出残渣乾燥粉末の配合により飼料中の魚粉配合率の削減が可能であることが明らかとなった。   From the above results, it was clarified that, in the same way as rainbow trout, it is possible to reduce the mixing ratio of fish meal in the feed by mixing dry dried powder of Chishimasa in the feed for sweetfish.

(マダイでの水槽試験) (Water tank test with red sea bream)

Figure 0005090074
Figure 0005090074

表9に示す6種類(飼料30〜飼料35)の配合内容のペレットタイプの試験飼料を作製した。   Pellet type test feeds having six types (feed 30 to feed 35) shown in Table 9 were prepared.

魚油を除く各原料を秤量・混合したのち、粉砕機でJIS標準篩32メッシュ以下に粉砕し、これに約5%の水を加えて混練し、ペレットマシーン(CPM社製・2HP・1/8インチダイ)を用いて直径約3.2mmのペレット状に成型し、これを熱風乾燥機を用いて乾燥したのちに、既定量の魚油を噴霧添加してハードペレット飼料を作製した。飼料30は対照飼料であり、飼料31〜飼料35は抽出残渣乾燥粉末を各々0.1重量%、0.5重量%、1重量%、2重量%、5重量%配合し、合計が100となるよう魚粉で調整した飼料である。   Each raw material excluding fish oil is weighed and mixed, then pulverized to JIS standard sieve 32 mesh or less with a pulverizer, added with about 5% water and kneaded, and pellet machine (CPM, 2HP, 1/8) Inch dies) were formed into pellets having a diameter of about 3.2 mm, dried using a hot air drier, and then a predetermined amount of fish oil was sprayed to prepare a hard pellet feed. Feed 30 is a control feed, and feed 31 to feed 35 are blended with 0.1% by weight, 0.5% by weight, 1% by weight, 2% by weight, and 5% by weight, respectively, and the total is 100. It is a feed adjusted with fish meal.

平均体重が45g前後のマダイを供試し、各試験飼料について、500リットル容のFRP製水槽6水槽を用いて、各水槽に体重を測定した30尾ずつを収容し、エアレーションを施して水温22℃の流水で飼育した。各飼料は朝夕の2回に分け、原則として日曜日を除く毎日、飽食給餌を行って摂餌量を記録した。試験期間は53日間で給餌日数は45日であった。試験終了日に各水槽内の供試魚の体重測定と尾数確認を行った。   A red sea bream with an average body weight of around 45 g was tested, and for each test feed, using a 500 liter FRP water tank 6 water tanks, 30 fish whose weights were measured were accommodated in each tank, aerated, and a water temperature of 22 ° C. Was raised in running water. Each feed was divided into two mornings and evenings, and in principle, every day except Sunday, a satiety feeding was performed and the amount of food intake was recorded. The test period was 53 days and the number of feeding days was 45 days. On the test end date, the weight of each test fish in each tank and the number of fish were confirmed.

Figure 0005090074
Figure 0005090074

試験の結果は表10のとおりであった。
対照の飼料30に較べて飼料32〜飼料34は、飼料効率と個体平均増重倍率のいずれにおいても優れ、特に抽出残渣乾燥粉末を1重量%配合した飼料33が最も優れていた。また、抽出残渣乾燥粉末を0.1重量%配合した飼料31は対照の飼料30とほぼ同等であったが、同乾燥粉末を5重量%配合した飼料35では飼料効率、個体平均増重倍率ともに劣る結果となった。
The test results are shown in Table 10.
Compared with the control feed 30, the feed 32 to the feed 34 were excellent in both feed efficiency and individual average weight gain, and the feed 33 in which 1% by weight of the extraction residue dry powder was blended was most excellent. In addition, feed 31 containing 0.1% by weight of the extracted residue dry powder was almost the same as control feed 30, but feed 35 containing 5% by weight of the dry powder had both feed efficiency and individual average weight gain. The result was inferior.

以上の結果から、ニジマス・アユと同様、マダイにおいてもチシマザサの抽出残渣乾燥粉末に飼料効率改善効果のあることと魚粉削減効果のあることが明らかとなった。   From the above results, it has been clarified that, like rainbow trout and ayu, red sea urchin extract residue dry powder has an effect of improving feed efficiency and an effect of reducing fish meal.

Claims (1)

チシマザサ及び/又はクマイザサの全乾燥物、抽出物及び抽出残渣あるいはその乾燥物の少なくとも1種以上を含有し、その含有率が、全乾燥物では0.01〜1重量%、抽出物では0.005〜0.1重量%、抽出残渣の乾燥物では0.05〜2重量%であることを特徴とする飼料効率改善用及び/又は魚粉配合率削減用鮭鱒類、アユ又はマダイ養殖用飼料。 It contains at least one of all dry matter, extract and extract residue or dry residue thereof, and the content is 0.01 to 1% by weight for the total dry matter, and 0.1% for the extract. Feed for improving feed efficiency and / or reducing fish meal blending rate , oysters, red sea bream, and red sea bream feed, characterized in that it is 005 to 0.1% by weight, and 0.05 to 2% by weight in the dry residue of the extraction residue .
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