JPS62278951A - Feed containing leaf protein for culture of aquatic animal - Google Patents

Abstract

PURPOSE:To obtain a feed for culture of aquatic animal such as sweetfish, young yellowtail, bream, snapping turtle, etc., having remarkable effect for improving the body color of the animal, by compounding a conventional culture feed with a leaf protein. CONSTITUTION:A conventional feed for culture of aquatic animal is compounded with a leaf protein such as concentrated extract of one or more kinds of leguminous pasture or gramineous pasture and preferably further with antarctic krill meal.

Description

Detailed Description of the Invention 3. Detailed Description of the Invention [Field of Industrial Application] The present invention provides an aquatic plant containing leaf protein for improving the body color of aquatic animals such as sweetfish, yellowtail, red sea bream, and stupa. This invention relates to feed for animal culture and feed for aquatic animal culture containing leaf protein and Antarctic krill meal.

[Conventional technology]

It is generally said that farmed fish has more fat and tastes worse than wild fish. The causes are thought to be feed composition factors and environmental factors such as lack of exercise. Among the differences between farmed fish and wild fish, the difference in body color has a particularly significant impact on market prices. Namely, Ayu,
In the natural world, aquatic animals such as yellowtail, red sea bream, and stinging turtle ingest carotenoid pigments that are widely distributed in plants and animals, giving them body colors such as yellow, orange, and red. They do not exhibit these bright colors because they are not fed feed that contains them.

For example, in the case of sweetfish, wild sweetfish have a bright yellow to orange band on the abdomen below the lateral line of the body due to ingestion of algae, while cultured sweetfish have a darkened body color.
Or it has a bluish tinge.

In addition, in the case of stupas, the abdomen of natural stinging turtles is yellow, whereas the abdomen of farmed stinging turtles is white.

Research has been conducted on improving the body color of cultivated fish necks by feeding them with various high carotenoid-containing feeds.
The body color of Japanese carp has been improved by using artificial feed containing Antarctic krill meal or spirulina, and for yellowtail, using artificial feed containing Antarctic krill meal.

Furthermore, research has been conducted on sweetfish, and it is known that when they are raised on artificial feed supplemented with Antarctic krill meal, a red band is observed on the abdomen below the lateral line of the body. It is also known that when fish are fed artificial feed supplemented with spirulina, a yellow-orange band that approaches the color of natural sweetfish is observed on the abdomen below the lateral line of the body, resulting in improved body color.

[Problem that the invention seeks to solve]

However, the various carotenoid-containing feeds that have been studied so far are expensive, and the color development time (the number of days it takes for a change in body color to appear after feeding a high-strength carotenoid-containing feed), The brightness, etc., were not sufficient, and the color tone could not be adjusted freely.

For example, Antarctic krill meal (see Aquaculture, December 1985 issue, ``About quality improvement of sweetfish'' by Akio Tsujimura and Kimio Akira) has red carotenoids as its main component, and generally gives only a red tinge to the body color. Spirulina (see JP-A-53-75095) contains the same components of yellow carotenoid and red carotenoid, and has a good body color improving effect, but is very expensive.

Therefore, there has been a need for a feed for aquatic animal cultivation that uses inexpensive raw materials to provide a bright color tone in a shorter color development time, and that can be adjusted to a color tone closer to natural colors depending on the variety of aquatic animals.

[Means for solving problems]

The present inventors have conducted various studies from the above viewpoint and have completed the present invention.

That is, the present invention is a feed for cultivating aquatic animals containing leaf protein and a feed for cultivating aquatic animals containing leaf protein and Antarctic krill meal.
It has a remarkable effect on improving the body color of red sea bream, stinging turtle, etc. In addition, leaf protein, which is the raw material, can be produced by hand as LPG (described later) at an extremely low cost (approximately one-tenth the price of spirulina), and if it is further blended with Antarctic krill meal, etc. You can adjust the color tone.

As the leaf protein used in the present invention, for example, LPC (LeafProt.
A green leaf protein concentrate called ein concentrates can be used (Japanese Journal of Poultry Science, Vol. 19, No. 1 "Nutritional and Economic Evaluation of Green Leaf Protein A Concentrate Prototyped with New Sealant" by Minoru Yoshida and Hoshii). (see Hiroshi). LPG was developed by New Sealant as a feed for poultry, and is a dark green product prepared by mechanically squeezing the mixed grass of ryegrass and white clover, then blowing steam into the extract and thermally coagulating it. powder. In addition, as grasses, LPG prepared using grasses such as timothy, orchard grass, etc., and legumes such as red clover, hairy vetch, etc.
can also be used.

The present inventors discovered that the intended purpose could be achieved by adding LPC, which had been conventionally considered as feed for poultry, to aquatic fodder feed, and completed the present invention. It's arrived.

In the present invention, the amount of LPC added is generally preferably in the range of 3 to 35%, more preferably in the range of 5 to 25%, based on the total weight of the feed. LPG3%
The following feeds have little effect on coloring, and at 35% or more, the coloring effect reaches a plateau compared to an increase in the amount added.

Furthermore, by adding Antarctic krill meal, the color tone of the animal's body surface can be adjusted.

In this case, although it depends on the type of animal, the ratio of LPG to Antarctic krill meal is preferably in the range of 0 to 25 near 5 by weight. If the Antarctic krill meal exceeds 25Nia5, the body color of sweetfish and the like will develop an excessive reddish tinge, which is undesirable.

〔Effect of the invention〕

According to the present invention, as shown in Example 1, it is possible to obtain sweetfish with a color tone and saturation extremely close to natural sweetfish, compared to conventional feeds using, for example, spirulina.

Furthermore, as shown in Example 2, in the case of turtles fed the same amount of LPG or spirulina, it takes 25 days for a change in body color to appear and 30 days for the color to change in the case of spirulina-containing feed. In contrast, with the LPG-containing feed of the present invention, a change in body color appears in just 15 days, and the color change is completed in 20 days. By the way, the carotenoid content of LPG is about half that of spirulina, so some trace components are involved in the feed of the present invention, and the body color improving effect of carotenoids is expressed with extremely high efficiency, shortening the color browning time. It is considered that

〔Example〕

EXAMPLES Next, the present invention will be explained in more detail with reference to Examples, but the present invention is not limited thereto.

Example 1 LPG (total rotinoid 1.560 mg/kg) and Antarctic krill meal (total rotinoid 125 mg/kg)
) and powdered feed were prepared according to the formulation shown in Table 1, formed into pellets using a pellet machine, and then crushed using a crumbler to obtain a crumple-shaped artificial feed for sweetfish.

As a comparative example, a mixture of spirulina and powdered feed containing 10% spirulina, 40% northern meal, 20% defatted wheat germ, 21% wheat flour, 8% soybean meal, 1% vitamins and minerals was prepared, and molded in the same manner as in the example. Crumple feed for sweetfish (X
) was obtained (total rotinoid 28.5 mg/100 g
, diet).

A breeding test was conducted by feeding sweetfish with the artificial feed for sweetfish prepared as described above. The number of reared fish is 2. O
Assuming 00 animals, A, B, C, D, EXF, GlH, I, J,
Artificial feeds A to J and X were each fed to each of the test plots in an amount of approximately 3% of the fish body weight. Ayu were reared in an aquarium. Ayu with an average weight of 26g was used, and the breeding period was June 3rd.
The average water temperature was 18°C for 30 days from day 0. Breeding was carried out smoothly, and the average fish weight in each area at the end of the day was 52-5.
It was 8g.

The effect of body color improvement was measured using a colorimeter to measure the yellow to reddish-orange band that appeared in part III below the lateral line between the head and the anterior base of the dorsal fin of the sweetfish body. The results are shown in Table 2.

Example 2 A breeding test was carried out by adding an equal amount of water to the feed for stinging turtles having the composition shown in Table 3 and feeding them as a bait.

The number of animals kept was 10 in each group, and each group was fed about 2% of their average body weight. Using a stupon with an average weight of 610g,
The animals were reared for 30 days from September 3rd at a water temperature of 27 to 29°C.

The coloring results are shown in Table 4.

Table 3: Contents of compound feed for stinging turtles *All numbers represent the ratio to the total weight of feed.

Claims (3)

[Claims] (1) Feed for aquatic animal cultivation containing leaf protein. (2) The feed according to claim (1), wherein the leaf protein is a concentrate extracted from one or more of leguminous grasses and gramineous grasses. (3) Feed for aquatic animal cultivation containing Antarctic krill meal and leaf protein.