JP4926946B2 - Ｐｄｘ１発現性内胚葉 - Google Patents

Ｐｄｘ１発現性内胚葉 Download PDF

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JP4926946B2
JP4926946B2 JP2007510879A JP2007510879A JP4926946B2 JP 4926946 B2 JP4926946 B2 JP 4926946B2 JP 2007510879 A JP2007510879 A JP 2007510879A JP 2007510879 A JP2007510879 A JP 2007510879A JP 4926946 B2 JP4926946 B2 JP 4926946B2
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cells
pdx1
cell
expression
endoderm
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JP2008503203A5 (cg-RX-API-DMAC7.html
JP2008503203A (ja
Inventor
ケビン アレン ダムール
アラン ディー アグルニック
スーザン エリアザール
エマニュエル イー べェツゲ
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ヴィアサイト，インコーポレイテッド
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
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  • Biomedical Technology (AREA)
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  • Genetics & Genomics (AREA)
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  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2007510879A 2004-04-27 2005-04-26 Ｐｄｘ１発現性内胚葉 Expired - Lifetime JP4926946B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US56629304P 2004-04-27 2004-04-27
US60/566,293 2004-04-27
US58656604P 2004-07-09 2004-07-09
US60/586,566 2004-07-09
US58794204P 2004-07-14 2004-07-14
US60/587,942 2004-07-14
US11/021,618 2004-12-23
US11/021,618 US7510876B2 (en) 2003-12-23 2004-12-23 Definitive endoderm
PCT/US2005/014239 WO2005116073A2 (en) 2004-04-27 2005-04-26 Pdx1 expressing endoderm

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2011147598A Division JP5669681B2 (ja) 2004-04-27 2011-07-01 Ｐｄｘ１発現性内胚葉

Publications (3)

Publication Number Publication Date
JP2008503203A JP2008503203A (ja) 2008-02-07
JP2008503203A5 JP2008503203A5 (cg-RX-API-DMAC7.html) 2008-06-19
JP4926946B2 true JP4926946B2 (ja) 2012-05-09

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ID=45319532

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Application Number Title Priority Date Filing Date
JP2007510879A Expired - Lifetime JP4926946B2 (ja) 2004-04-27 2005-04-26 Ｐｄｘ１発現性内胚葉
JP2011147598A Expired - Lifetime JP5669681B2 (ja) 2004-04-27 2011-07-01 Ｐｄｘ１発現性内胚葉
JP2014168939A Expired - Lifetime JP6158152B2 (ja) 2004-04-27 2014-08-22 Ｐｄｘ１発現性内胚葉
JP2015111656A Expired - Lifetime JP6265942B2 (ja) 2004-04-27 2015-06-01 Ｐｄｘ１発現性内胚葉
JP2017026645A Expired - Lifetime JP6605520B2 (ja) 2004-04-27 2017-02-16 Ｐｄｘ１発現性内胚葉
JP2018167312A Expired - Lifetime JP6709262B2 (ja) 2004-04-27 2018-09-06 Ｐｄｘ１発現性内胚葉

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Application Number Title Priority Date Filing Date
JP2011147598A Expired - Lifetime JP5669681B2 (ja) 2004-04-27 2011-07-01 Ｐｄｘ１発現性内胚葉
JP2014168939A Expired - Lifetime JP6158152B2 (ja) 2004-04-27 2014-08-22 Ｐｄｘ１発現性内胚葉
JP2015111656A Expired - Lifetime JP6265942B2 (ja) 2004-04-27 2015-06-01 Ｐｄｘ１発現性内胚葉
JP2017026645A Expired - Lifetime JP6605520B2 (ja) 2004-04-27 2017-02-16 Ｐｄｘ１発現性内胚葉
JP2018167312A Expired - Lifetime JP6709262B2 (ja) 2004-04-27 2018-09-06 Ｐｄｘ１発現性内胚葉

Country Status (14)

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EP (1) EP1740612B1 (cg-RX-API-DMAC7.html)
JP (6) JP4926946B2 (cg-RX-API-DMAC7.html)
KR (2) KR101395516B1 (cg-RX-API-DMAC7.html)
CN (1) CN103103158B (cg-RX-API-DMAC7.html)
AU (1) AU2005247869B2 (cg-RX-API-DMAC7.html)
CA (1) CA2564114C (cg-RX-API-DMAC7.html)
DK (2) DK1740612T3 (cg-RX-API-DMAC7.html)
EA (1) EA011953B1 (cg-RX-API-DMAC7.html)
ES (2) ES2801001T3 (cg-RX-API-DMAC7.html)
IL (1) IL178847A (cg-RX-API-DMAC7.html)
MX (1) MXPA06012445A (cg-RX-API-DMAC7.html)
NZ (1) NZ550605A (cg-RX-API-DMAC7.html)
SG (1) SG152273A1 (cg-RX-API-DMAC7.html)
WO (1) WO2005116073A2 (cg-RX-API-DMAC7.html)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007059007A2 (en) 2005-11-14 2007-05-24 Cythera, Inc. Markers of definitive endoderm
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
SG149061A1 (en) 2003-12-23 2009-01-29 Cythera Inc Definitive endoderm
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
WO2006016999A1 (en) * 2004-07-09 2006-02-16 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
CA2576872C (en) 2004-08-13 2013-11-12 University Of Georgia Research Foundation, Inc. Compositions and methods for self-renewal and differentiation in human embryonic stem cells
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
EP1838843B1 (en) * 2004-12-23 2019-07-10 Viacyte, Inc. Expansion of definitive endoderm cells
US20060194321A1 (en) * 2005-01-31 2006-08-31 Alan Colman Directed differentiation of embryonic stem cells and uses thereof
WO2006130851A2 (en) * 2005-06-02 2006-12-07 Arizona Board Of Regents On Behalf Of The University Of Arizona Prevascularized devices and related methods
CN101484575B (zh) 2005-06-08 2013-10-02 森托科尔公司 用于眼变性的细胞疗法
AU2012202481B2 (en) * 2005-10-27 2015-07-02 Viacyte, Inc. PDX1-expressing dorsal and ventral foregut endoderm
ES2743202T3 (es) 2005-10-27 2020-02-18 Viacyte Inc Endodermo de intestino proximal dorsal y ventral que expresa PDX1
CA2643478C (en) 2006-02-23 2019-06-18 Novocell, Inc. Compositions and methods useful for culturing differentiable cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
EP4112718A1 (en) * 2006-03-02 2023-01-04 ViaCyte, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US11254916B2 (en) 2006-03-02 2022-02-22 Viacyte, Inc. Methods of making and using PDX1-positive pancreatic endoderm cells
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US7989204B2 (en) 2006-04-28 2011-08-02 Viacyte, Inc. Hepatocyte lineage cells
EP2021462B1 (en) * 2006-04-28 2019-01-09 Lifescan, Inc. Differentiation of human embryonic stem cells
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
CA2695225C (en) 2007-07-31 2021-06-01 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
CA2954431C (en) * 2007-11-27 2021-08-24 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic cells
CN101910196A (zh) 2007-12-28 2010-12-08 诺沃-诺迪斯克有限公司 Ddr1-介导的从它们的祖细胞向胰腺内分泌细胞的细胞纯化
CN102046779A (zh) 2008-02-21 2011-05-04 森托科尔奥索生物科技公司 用于细胞粘附、培养和分离的方法、表面改性培养板和组合物
US8399251B2 (en) 2008-04-03 2013-03-19 Novo Nordisk A/S Use of the extracellular marker DNER for identification and selection of specific pancreatic cells
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
US7939322B2 (en) 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
EP2942392B1 (en) 2008-06-30 2018-10-03 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
RU2011103183A (ru) * 2008-06-30 2012-08-10 Сентокор Орто Байотек Инк. (Us) Дифференцирование плюрипотентных стволовых клеток
MX2011004565A (es) 2008-10-31 2011-07-28 Centocor Ortho Biotech Inc Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico.
ES2727950T3 (es) 2008-10-31 2019-10-21 Janssen Biotech Inc Diferenciación de células madre embrionarias humanas en linaje endocrino pancreático
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
AU2009313870B2 (en) 2008-11-14 2013-07-11 Viacyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
KR101774546B1 (ko) 2008-11-20 2017-09-04 얀센 바이오테크 인코포레이티드 마이크로-캐리어 상의 만능 줄기 세포 배양
JP5719305B2 (ja) 2008-11-20 2015-05-13 ヤンセン バイオテツク，インコーポレーテツド 平面支持体上での細胞付着及び培養のための方法及び組成物
CN102482640B (zh) 2009-07-20 2015-03-11 詹森生物科技公司 人胚胎干细胞的分化
BR112012001480A2 (pt) 2009-07-20 2015-09-01 Janssen Biotech Inc Diferenciação de células-tronco embriônicas humanas
CN103952372B (zh) 2009-07-20 2016-10-05 詹森生物科技公司 人胚胎干细胞的分化
ES2633648T3 (es) 2009-12-23 2017-09-22 Janssen Biotech, Inc. Diferenciación de células madre embrionarias humanas
MX343786B (es) 2009-12-23 2016-11-22 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas.
US12441985B2 (en) 2009-12-23 2025-10-14 Janssen Biotech, Inc. Methods for making insulin in vivo
SG10201501503VA (en) 2010-03-01 2015-04-29 Janssen Biotech Inc Methods for purifying cells derived from pluripotent stem cells
WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
EP3498825A1 (en) 2010-05-12 2019-06-19 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
ES2585028T3 (es) 2010-08-31 2016-10-03 Janssen Biotech, Inc. Diferenciación de células madre pluripotentes
CA2809300A1 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
EP3211070A1 (en) 2010-08-31 2017-08-30 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
JP5888852B2 (ja) * 2010-12-08 2016-03-22 学校法人近畿大学 免疫不全動物を用いた細胞の製法
JP2012143229A (ja) * 2010-12-24 2012-08-02 Chiba Univ 多能性幹細胞培養用組成物およびその用途
US20140234963A1 (en) 2011-06-21 2014-08-21 Novo Nordisk A/S Efficient induction of definitive endoderm from pluripotent stem cells
MX2014007744A (es) 2011-12-22 2015-01-12 Janssen Biotech Inc Diferenciacion de celulas madre embrionicas humanas en celulas individuales positivas de insulina hormonal.
MX354775B (es) 2012-03-07 2018-03-20 Janssen Biotech Inc Medios definidos para la expansion y el mantenimiento de celulas madre pluripotentes.
DK3450542T3 (da) 2012-06-08 2021-11-01 Janssen Biotech Inc Differentiering af humane embryonale stamceller til endokrine pancreatiske celler
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
RU2687379C2 (ru) 2012-12-31 2019-05-13 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки
ES2942484T3 (es) 2012-12-31 2023-06-01 Janssen Biotech Inc Diferenciación de células madre embrionarias humanas en células endocrinas pancreáticas usando reguladores de HB9
US10344264B2 (en) 2012-12-31 2019-07-09 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
JP6588969B2 (ja) 2014-05-16 2019-10-09 ヤンセン バイオテツク，インコーポレーテツド 膵内分泌細胞内のｍａｆａ発現を強化するための小分子の使用
ES2860423T3 (es) 2014-05-28 2021-10-05 Childrens Hospital Med Ct Métodos y sistemas para convertir células precursoras en tejidos gástricos mediante diferenciación dirigida
WO2016061464A1 (en) 2014-10-17 2016-04-21 Children's Hospital Center, D/B/A Cincinnati Children's Hospital Medical Center In vivo model of human small intetine using pluripotent stem cells and methods of making and using same
CA2983764A1 (en) 2015-04-24 2016-10-27 University Of Copenhagen Isolation of bona fide pancreatic progenitor cells
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
JP6963882B2 (ja) 2016-05-05 2021-11-10 チルドレンズ ホスピタル メディカル センター 胃底部組織のインビトロでの製造のための方法及び当該方法と関連した組成物
EP3534907A4 (en) 2016-11-04 2020-06-24 Children's Hospital Medical Center COMPOSITIONS AND METHODS FOR TREATING HEPATIC DISEASE
EP3548507A4 (en) 2016-12-05 2020-07-15 Children's Hospital Medical Center COLON ORGANOIDS AND PROCESSES FOR THE PREPARATION AND USE THEREOF
EP3609997A4 (en) 2017-04-14 2021-03-03 Children's Hospital Medical Center COMPOSITIONS OF STEM CELLS FROM MULTIPLE DONOR CELLS AND THEIR PREPARATION PROCEDURES
CA3077442A1 (en) 2017-10-10 2019-04-18 Children's Hospital Medical Center Esophageal tissue and/or organoid compositions and methods of making same
CA2983845C (en) 2017-10-26 2024-01-30 University Of Copenhagen Generation of glucose-responsive beta cells
WO2019126626A1 (en) 2017-12-21 2019-06-27 Children's Hospital Medical Center Digitalized human organoids and methods of using same
SG11202100533VA (en) 2018-07-26 2021-02-25 Childrens Hospital Med Ct Hepato-biliary-pancreatic tissues and methods of making same
CA3112026A1 (en) 2018-09-12 2020-03-19 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
US20220234006A1 (en) 2019-05-31 2022-07-28 W. L. Gore & Associates, Inc. A biocompatible membrane composite
CA3139292C (en) 2019-05-31 2025-06-10 Viacyte, Inc. CELL ENCAPSULATION DEVICES WITH REGULATED OXYGEN DIFFUSION DISTANCES
CA3139585C (en) 2019-05-31 2024-01-23 W. L. Gore & Associates, Inc. A biocompatible membrane composite
US12497597B2 (en) 2019-05-31 2025-12-16 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
IL300361A (en) 2020-08-15 2023-04-01 Regeneron Pharma will fall into obesity in patients with variants of nucleic acid compounds encoding the calcitonin receptor

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005097977A2 (en) * 2004-04-01 2005-10-20 Wisconsin Alumni Research Foundation Differentiation of stem cells to endoderm and pancreatic lineage

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3945846B2 (ja) * 1995-11-09 2007-07-18 武田薬品工業株式会社 膵臓機能改善剤
US6331406B1 (en) * 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
US6326201B1 (en) * 1999-02-10 2001-12-04 Curis, Inc. Pancreatic progenitor cells, methods and uses related thereto
JP4426064B2 (ja) * 2000-05-31 2010-03-03 独立行政法人科学技術振興機構 脊椎動物の膵臓のインビトロ形成方法
CA2415108A1 (en) * 2000-06-30 2002-01-10 Amcyte, Inc. Culturing pancreatic stem cells having a specified, intermediate stage of development
AU2001286173B2 (en) * 2000-08-01 2007-10-25 Yissum Research Development Company Directed differentiation of embryonic cells
CA2430627A1 (en) * 2000-11-30 2002-12-27 Stemron, Inc. Isolated homozygous stem cells differentiated cells derived therefrom and materials and methods for making and using same
CA2435826A1 (en) * 2001-01-24 2002-08-01 The Government Of The United States Of America Differentiation of stem cells to pancreatic endocrine cells
JP2004531262A (ja) * 2001-05-15 2004-10-14 ラッパポート ファミリー インスチチュート フォア リサーチ イン ザ メディカル サイエンシズ ヒト胚性幹細胞由来インスリン産生細胞
JP2005515753A (ja) * 2001-05-25 2005-06-02 サイセラ，インコーポレイテッド 幹細胞分化
AU2002312514A1 (en) * 2001-06-14 2003-01-02 Regents Of The University Of California Hoxd3 compositions ad methods for improved wound healing
EP1298201A1 (en) * 2001-09-27 2003-04-02 Cardion AG Process for the production of cells exhibiting an islet-beta-cell-like state
MXPA04005010A (es) * 2001-11-26 2005-04-08 Advanced Cell Tech Inc METODO PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS METODOS PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS HUMANOS REPROGRAMADOS Y CELULAS DEL TALLO HUMANAS AUTOLOGAS E ISOGENICAS.
CN1602351B (zh) * 2001-12-07 2011-03-30 杰龙公司 源自人胚胎干细胞的胰岛细胞
US20030138949A1 (en) * 2001-12-12 2003-07-24 Anil Bhushan Methods for the regeneration of pancreatic islets and expansion of pancreatic endocrine cells
US20030162290A1 (en) * 2002-01-25 2003-08-28 Kazutomo Inoue Method for inducing differentiation of embryonic stem cells into functioning cells
JP2005523328A (ja) * 2002-04-19 2005-08-04 ユニバーシティ オブ ピッツバーグ オブ ザ コモンウェルス システム オブ ハイヤー エデュケーション 胎盤由来の幹細胞及びその使用
EP1516925A1 (en) * 2003-09-18 2005-03-23 Fundacion IVI para el Estudio de la reproduccion Humana (FIVIER) Generation of human embryonic stem cells from triploid zygotes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005097977A2 (en) * 2004-04-01 2005-10-20 Wisconsin Alumni Research Foundation Differentiation of stem cells to endoderm and pancreatic lineage

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