JP4892949B2 - Dentifrice composition - Google Patents

Description

  The present invention relates to a dentifrice composition having excellent dextranase stability in a preparation, good foaming properties, and an excellent plaque formation inhibitory effect.

  Dextranase is blended as an active ingredient in a composition for oral cavity because a dental caries prevention effect can be obtained by decomposing plaque. On the other hand, in the composition for oral cavity, a surfactant is blended from the viewpoint of usability and usability, and in particular, since a large amount of abrasive is blended in the dentifrice composition, sufficient foaming properties are obtained. An anionic surfactant is mainly blended for securing. For this reason, a technique for stabilizing dextranase which is an enzyme in the preparation is indispensable, and it is known that it is useful to add a specific non-anionic surfactant for stabilization. For example, a carboxylic acid type amphoteric surfactant (Patent Document 1), a polyoxyethylene alkyl ether (Patent Document 2), a glycerin fatty acid ester (Patent Document 3), an example of blending fatty acid diethanolamide, etc. (Patent Document 4) Is mentioned.

  However, when a large amount of nonionic surfactant is blended, the excellent foaming property due to the anionic surfactant tends to be lowered and the effect of other active ingredients such as bactericides tends to be inhibited, and these problems are solved. Therefore, there has been a demand for a dentifrice composition that has a high stability of dextranase, an excellent feeling of use such as foaming property, and an excellent effect of suppressing plaque.

  On the other hand, as an example of an oral composition containing a fatty acid amidopropyl betaine and a cationic fungicide, the oral cavity capable of effectively preventing plaque formation by improving the retention of the cationic fungicide on the tooth surface. Composition (Patent Document 5), composition for oral cavity (Patent Document 6) having extremely high chemical cleaning power for removing dirt on the teeth attached and deposited on the tooth surface, oral cavity containing cationic germicide and crystalline cellulose Composition (Patent Document 7), an inhibitor for oral cavity (Patent Document 8) for inhibiting periodontal disease, which inhibits the activity of LPS (lipoprotein) and is effective in the prevention or treatment of periodontal disease.

  However, in these documents, by combining a specific fatty acid amidopropyl betaine and a cationic fungicide at a specific mass ratio, the stability of dextranase is excellent and the foaming at the time of use is excellent, and further remarkable There is no description about obtaining a dentifrice composition having an enhanced effect of inhibiting plaque formation.

JP 58-225007 A JP-A 61-176518 Japanese Patent Laid-Open No. 03-181413 Japanese Patent Laid-Open No. 08-12543 JP 09-12437 A JP 2000-34213 A JP 2002-179541 A JP 2005-104913 A

  The present invention has been made in view of the above circumstances, and provides a dentifrice composition having excellent dextranase stability, good foaming properties, excellent usability, and remarkably excellent plaque formation inhibiting effect. With the goal.

In view of the above circumstances, the present inventor is to develop a dentifrice composition that is excellent in stability of dextranase in a preparation, excellent in foaming properties, excellent in feeling of use, and further remarkably excellent in plaque formation inhibitory effect. As a result of diligent investigation for the purpose of (A), (B) a fatty acid amidopropyl betaine having an alkyl chain length of 12 to 14 carbon atoms is added to a dentifrice composition containing an abrasive containing (A) dextranase. and .5～2.0 mass%, and 0.01 to 0.1% by weight of (C) cationic biocides, and 10 to 150 at the mixing ratio is the weight ratio of (B) / (C) ranges in blended with the addition of pH adjusting agent, if necessary, be pH range of 6.5 to 8.5 of the composition at 25 ° C., by more preferably not blended anionic surfactant Improves the stability of dextranase and foams when used It was found that the standing was good and that the effect of suppressing the formation of plaque was remarkably enhanced by the synergistic action of these components, which led to the present invention.

Accordingly, the present invention provides (A) a dentifrice composition containing an abrasive containing dextranase and (B) a fatty acid amidopropyl betaine having an alkyl chain length of 12 to 14 carbon atoms of 0.5 to 2 and .0% by mass, (C) cetylpyridinium chloride, benzalkonium chloride, and 0.01 to 0.1 wt% of one or more cationic bactericidal agent selected from benzethonium chloride, (B) / (C ) is added in amounts to be 10 to 150 at a mass ratio, and provide a dentifrice composition characterized in that the pH range of 6.5 to 8.5 of the composition at 25 ° C..

  The dentifrice composition of the present invention has excellent dextranase stability, good foaming and excellent usability, and is remarkably excellent in plaque formation inhibitory effect, preventing or treating caries and periodontal disease. Useful for.

Hereinafter, the present invention will be described in more detail. The dentifrice composition of the present invention is a dentifrice composition containing (A) dextranase and containing an abrasive, and (B) an alkyl chain having a carbon number. 12-14 fatty acid amidopropyl betaine and (C) cationic fungicide are mix | blended , (B) / (C) compounding ratio and pH of a composition are a specific range.

  Here, as (A) dextranase, a dextran obtained by a known method from a known dextranase-producing bacterium belonging to the genus Ketomium, Penicillium, Aspergillus, Spicaria, Lactobacillus, Servibrio, etc. In addition to nuclease, dextranase produced from other microorganisms can also be used, and commercially available products such as those manufactured by Sankyo Corporation can be used.

  The blending amount of dextranase is 2 to 200 units / g, and particularly preferably 10 to 50 units / g. When the amount is less than 2 units / g, a sufficient plaque removing effect cannot be obtained. Here, 1 unit of dextranase is the amount of dextranase that produces free reducing sugar corresponding to 1 μmol of glucose per minute when the reaction is carried out using dextran as a substrate.

(B) As fatty acid amidopropyl betaine, fatty acid amidopropyl betaine having an alkyl chain length of 12 to 14 carbon atoms is used, and among them, lauric acid amidopropyl betaine and palm oil fatty acid amidopropyl betaine are good. .
These fatty acid amidopropyl betaines are trade names such as Amphitol 20AB (Kao Corporation), Amphitol 55AB (Kao Corporation), Softazoline LSB (Kawaken Fine Chemicals Corporation), TEGO BETAIN CK (Goldschmidt Co., Ltd.), etc. It is available.

  The blending amount of the fatty acid amidopropyl betaine is 0.5 to 2.0% (mass%, the same applies hereinafter) of the whole composition, and preferably 0.7 to 1.5%. If the blending amount is less than 0.5%, sufficient foaming property cannot be obtained. If the blending amount exceeds 2.0%, the dextranase stability is deteriorated and the irritation to the tongue is increased. .

(C) As a cationic bactericidal agent , among compounds having a bactericidal effect including quaternary ammonium salts such as alkylpyridinium salts, benzyl long-chain alkyl short-chain dialkylammonium salts and derivatives thereof, it is common in oral compositions. Cetylpyridinium chloride, benzalkonium chloride, and benzethonium chloride are preferably used from the feeling of use such as bactericidal power and taste.
These cationic fungicides can be obtained from Wako Pure Chemical Industries, Ltd., Kanto Chemical Co., Ltd., Tokyo Chemical Industry Co., Ltd. and the like.

  The compounding quantity of a cationic disinfectant is 0.01 to 0.1% of the whole composition, Preferably it is 0.02 to 0.05%. If the blending amount is less than 0.01%, a sufficient plaque formation inhibitory effect cannot be obtained.

  In the present invention, in order to obtain a sufficient plaque formation inhibitory effect, the blending ratio ((B) / (C)) of (B) fatty acid amidopropyl betaine to (C) cationic fungicide is 10 to 150 by mass ratio. Preferably, it is 15-50, More preferably, it is 15-35. When the ratio of (B) / (C) is less than 10, sufficient foamability cannot be obtained, and when it exceeds 150, a sufficient plaque formation inhibitory effect cannot be obtained.

  In the present invention, it is important for ensuring the stability of dextranase that the pH of the preparation is 6.5 to 8.5, preferably 7.0 to 8.0, and the pH is less than 6.5 or pH 8 If it exceeds .5, dextranase may be remarkably inactivated, and the plaque formation inhibitory effect decreases accordingly. In addition, pH adjustment can be adjusted using a pH adjuster as needed besides the course of composition. Examples of the pH adjuster include sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium hydroxide, potassium hydroxide, citric acid, malic acid, lactic acid, tartaric acid, acetic acid, phosphoric acid, pyrophosphoric acid, glycerophosphoric acid, and these. And various salts.

The dentifrice composition of the present invention can be prepared in a dosage form such as toothpaste, liquid dentifrice, liquid dentifrice, and moisturized dentifrice, and as an optional component within the range not impairing the effects of the present invention in addition to the above essential components and abrasives Other additives can be blended. For example thickeners, Nebayuizai, surfactants, sweetening agents, preservatives, can blend the active ingredient, dyes, perfumes, mixing the these components with water, can be prepared in a conventional manner.

  As abrasives, dicalcium phosphate dihydrate, dibasic calcium phosphate anhydrate, calcium pyrophosphate, aluminum hydroxide, alumina, titanium dioxide, crystalline zirconium silicate, polymethyl methacrylate, insoluble calcium metaphosphate, light Calcium carbonate, heavy calcium carbonate, magnesium carbonate, tribasic magnesium phosphate, zeolite, zirconium silicate, tricalcium phosphate, hydroxyapatite, fluoroapatite, calcium deficient apatite, tricalcium phosphate, tetracalcium phosphate, eighth calcium phosphate, synthesis Resin-based abrasives, precipitated silica, silica gel, aluminosilicate, zirconosilicate and the like can be mentioned. Among these abrasives, it is preferable to use an abrasive selected from silica-based abrasives and aluminum hydroxide from the viewpoint of the stability of dextranase. The compounding quantity of these abrasive | polishing agents is 10-50% of the whole composition normally.

  As the thickener, one kind or two or more kinds of polyhydric alcohols such as glycerin, sorbit, propylene glycol, polyethylene glycol having a molecular weight of 200 to 6000, ethylene glycol, and reduced starch saccharified product are used. Can be used in combination. 10-40% of the whole composition is suitable for the compounding quantity of a thickener.

  Binders include sodium alginate, propylene glycol alginate, carboxymethyl cellulose, polyvinyl alcohol, hydroxyethyl cellulose, sodium polyacrylate, xanthan gum, carbopol, guar gum, gelatin, Avicel, montmorillonite, kaolin And inorganic binders such as bentonite. The compounding quantity of a binder is 0.5 to 3% of the whole composition normally.

As the surfactant, in addition to the fatty acid amidopropyl betaine which is a constituent requirement, other surfactants, for example, anionic surfactants, amphoteric surfactants other than the fatty acid amidopropyl betaine, as long as the effects of the present invention are not hindered. Nonionic surfactants can be added. For example, as an anionic surfactant, amphoteric surfactants such as lauryl sulfate, lauroyl sarcosine salt, myristyl sarcosine salt, polyoxyethylene alkyl sulfate, N-lauroyl taurate, α-olefin sulfonate, N-acyl glutamate, 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine, nonionic surfactant, for example, alkyl glycoside, sucrose fatty acid ester, alkylol amide, polyoxyethylene sorbitan monostearate , Polyoxyethylene polyoxypropylene glycol, polyoxyethylene alkyl ether, decaglyceryl laurate, polyoxyethylene hydrogenated castor oil, and the like.
In the present invention, when an anionic surfactant is blended as these other surfactants, the effect of the cationic bactericide may be reduced. It is.

  The blending amount of the other surfactants described above can be 0 to 3% of the total composition, and the total blending amount of the surfactant including the fatty acid amidopropyl betaine which is the component (B) of the present invention is 0.5 to 5% of the total composition is preferred.

  Sweeteners include sodium saccharin, aspartame, stevioside, stevia extract, paramethoxycinnamic aldehyde, neohesperidyl dihydrochalcone, perilartin, etc., and preservatives include parabens such as butylparaben and ethylparaben, paraoxybenzoates And sodium benzoate.

  Various active ingredients include fluorine compounds such as sodium fluoride, potassium fluoride, stannous fluoride, strontium fluoride and sodium monofluorophosphate, and water-soluble phosphate compounds such as potassium salt and sodium salt of orthophosphoric acid. , Tranexamic acid, epsilon-aminocaproic acid, allantoinchlorohydroxyaluminum, hinokitiol, ascorbic acid, sodium chloride, dl-tocopherol acetate, dihydrocholesterol, α-bisabolol, triclosan, isopropylmethylphenol, azulene, glycyrrhetin, glycyrrhetic acid, copper chlorophyllin sodium , Chelating phosphate compounds such as chlorophyll, glycerophosphate, copper compounds such as copper gluconate, aluminum lactate, strontium chloride, potassium nitrate, berberi , Hydroxamic acid and its derivatives, sodium pyrophosphate, sodium tripolyphosphate, zeolite, methoxyethylene, maleic anhydride copolymer, polyvinylpyrrolidone, epidihydrocholesterin, benzethonium chloride, sodium chloride, dihydrocholesterol, trichlorocarbanilide, Examples include zinc acid extract, soft sugar beet extract, buckwheat extract, camille, clove, rosemary, hornon, safflower and the like. The compounding quantity of an active ingredient can be made into an effective quantity in the range which does not prevent the effect of this invention.

  Perfumes are peppermint oil, spearmint oil, anise oil, eucalyptus oil, wintergreen oil, cassia oil, clove oil, thyme oil, sage oil, lemon oil, orange oil, peppermint oil, cardamom oil, coriander oil, mandarin oil, lime Oil, lavender oil, rosemary oil, laurel oil, camomil oil, caraway oil, marjoram oil, bay oil, lemongrass oil, origanum oil, pine needle oil, neroli oil, rose oil, jasmine oil, iris concrete, absolute peppermint Natural fragrances such as absolute rose and orange flower, and fragrances processed with these natural fragrances (front reservoir cut, rear reservoir cut, fractional distillation, liquid-liquid extraction, essence, powder fragrance, etc.), and menthol , Carvone, anethole, cineol, methyl salicylate Synamic aldehyde, eugenol, 3-l-mentoxypropane-1.2-diol, thymol, linalool, linalyl acetate, limonene, menthone, menthyl acetate, N-substituted-paramentane-3-carboxamide, pinene, octylaldehyde, Citral, pulegone, carbyl acetate, anisaldehyde, ethyl acetate, ethyl butyrate, allylcyclohexane propionate, methyl anthranilate, ethyl methyl phenyl glycidate, vanillin, undecalactone, hexanal, ethyl alcohol, propyl alcohol, butanol , Isoamyl alcohol, hexenol, dimethyl sulfide, cycloten, furfural, trimethylpyrazine, ethyl lactate, methyl lactate, Single-flavored ingredients such as tilthioacetate, as well as strawberry flavor, apple flavor, banana flavor, pineapple flavor, grape flavor, mango flavor, butter flavor, milk flavor, fruit mix flavor, tropical fruit flavor, etc. The well-known fragrance | flavor raw material used for a composition can be used, It is not limited to the fragrance | flavor of an Example.

  Moreover, although a compounding quantity is not specifically limited, It is preferable to use said fragrance | flavor raw material 0.000001 to 1% in a formulation composition. Moreover, as a fragrance | flavor for fragrance | flavor using the said fragrance | flavor raw material, it is preferable to use 0.1 to 2.0% in a formulation composition.

  As colorants, Red No. 2, Red No. 3, Red No. 225, Red No. 226, Yellow No. 4, Yellow No. 5, Yellow No. 205, Blue No. 1, Blue No. 2, Blue No. 201, Blue No. 204, Green No. 3 is exemplified. In addition, the compounding quantity of these components can be made into a normal quantity in the range which does not prevent the effect of this invention.

  EXAMPLES Hereinafter, although an experimental example, an Example, and a comparative example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to the following Example. In the following examples, the blending amount is mass%.

[Experimental example]
In the composition shown in Tables 1 and 2, dextranase, fatty acid amidopropyl betaine, a cationic bactericidal agent were blended, and a dentifrice composition having a formulation pH of 6.5 to 8.5 was prepared by the following method. A laminate tube having a diameter of 26 mm whose innermost layer is made of linear low-density polyethylene was filled with 50 g, and dextranase stability, the effect of suppressing plaque formation, and foaming were evaluated by the following methods.

Preparation of test dentifrice composition:
Preparation of the dentifrice composition was carried out by dissolving a water-soluble substance such as a cationic bactericide, sodium saccharin, sorbite solution and a pH adjuster as necessary in purified water, and then dispersing a binder such as sodium alginate. Propylene glycol solution was added and stirred. Then, the fragrance | flavor, the abrasive | polishing agent, the dextranase, and the fatty-acid amide propyl betaine were added in order, and also it stirred under reduced pressure (40 mmHg), and obtained the dentifrice composition. In addition, a unimixer (FM-SR-25, POWEREX CORPORATION) was used for the production.

  These dentifrice compositions were prepared using dextranase (Sankyo Co., Ltd.), lauric acid amidopropyl betaine (Amphithol 20AB (Kao Co., Ltd.), palm oil fatty acid amidopropyl betaine (TEGO BETAINN CK D, Goldschmidt). ), Cetylpyridinium chloride (Wako Pure Chemical Industries, Ltd.), benzalkonium chloride (Wako Pure Chemical Industries, Ltd.) Amphithol 20AB is a 30% aqueous solution, but lauric acid of Examples and Comparative Examples The amount of amidopropyl betaine was shown as a pure component value, in addition, sodium alginate, xanthan gum, sodium polyacrylate, sodium lauryl sulfate, sorbite, aluminum hydroxide, silicic anhydride, propylene glycol, sodium saccharin, sodium fluoride, Diphosphate Sodium hydrogen, disodium hydrogen phosphate, water for. Sorbitol with Standards of Cosmetic Ingredients standard products, and to prepare the dentifrice composition with 70% aqueous product showed the amount of 70% aqueous solution.

For the measurement of pH, a dentifrice sample that had been kept at a constant temperature of 25 ° C. was placed in a plastic container, the pH electrode that had been calibrated was directly inserted into the dentifrice, and the reading value after 3 minutes was taken as the pH of this sample. In addition, the following apparatus was used for pH measurement.
pH meter: MP220 (manufactured by METTLER TOLEDO)
pH electrode: Type InLab410

(1) Evaluation method of residual ratio of dextranase (dextranase stability) The dentifrice composition is a laminated tube (LDPE55 / PET12 / LDPE20 / white LDPE60 / EMAA20 / AL10 / EMAA30 / LDPE20 / LLDPE30 (manufactured by Dai Nippon Printing Co., Ltd.)) and stored at 45 ° C. for 2 weeks, 0.6 g of a dentifrice formulation was suspended in 15 ml of 0.1 M phosphate buffer, The centrifugal supernatant was used as a test solution. 1 ml of this test solution was added to 2 ml of a 1% dextran solution, reacted in a thermostatic bath at 35 ° C. for exactly 10 minutes, and the amount of reducing sugar produced was measured using the Somogy Nelson method. One unit of dextranase was defined as the amount of dextranase that produces free reducing sugar corresponding to 1 μmol of glucose per minute, and the average of three repetitions was evaluated according to the following criteria. The abbreviations and names in the layer structure of the laminated tube used are: LDPE: low density polyethylene, white LDPE: white low density polyethylene, LLDPE: linear low density polyethylene, AL: aluminum, EAA: ethylene / acrylic acid copolymer Resin, PET: Polyethylene terephthalate, EMAA: Copolymer resin of ethylene / methacrylic acid, and the number following the abbreviation indicates the thickness (μm) of each layer.
Score standard for dextranase stability ◎: dextranase remaining rate of 90% or more ○: dextranase remaining rate of 80% or more and less than 90% △: dextranase remaining rate of 60% or more and less than 80% ×: dextranase remaining rate Less than 60%

(2) Evaluation of foaming at the time of dentifrice It evaluated by the sensory test using ten expert panelists. About 1.5 cm of the test dentifrice composition was placed on a toothbrush and was used in a normal brushing method. The foaming of the dentifrice composition in the oral cavity during use was evaluated according to the following criteria.
4 points: The amount of foaming is sufficient and the feeling of use is excellent.
3 points: The amount of foaming is moderate and the feeling of use is satisfactory.
2 points: The amount of foaming is small and the feeling of use is slightly inferior.
1 point: There is almost no foaming and the feeling of use is bad.
The evaluation results of 10 persons were averaged, and those that ensure foaming of ◎ and ◯ on the following criteria were judged to be dentifrice compositions that can provide good foaming during dentifrice.
◎: Bubbling in the oral cavity is 3.5 to 4.0 points ○: Bubbling in the oral cavity is 3.0 to less than 3.5 △: Bubbling in the oral cavity is 2.0 to 3 Less than 0 point x: Foaming in the oral cavity is 1.0 point or more and less than 2.0 point

(3) Evaluation method of plaque formation inhibitory effect Dentifrice composition is artificial saliva (final concentrations are 50 mM KCl, 1 mM KH ₂ PO ₄ , 1 mM CaCl ₂ , 0.1 mM MgCl _2), and pH is 7.0 with KOH. The sample was diluted 4 times with distilled water to make 1 L with distilled water), and a hydroxyapatite (HA) plate having a diameter of 7 mm and a height of 3.5 mm was immersed at 37 ° C. for 3 minutes. The HA plate was taken out from the sample solution, transferred to a liquid medium (BHI) containing 1% saccharose to which about 1 × 10 ⁷ cells / ml of S. mutans 10449 was added, and incubated at 37 ° C. for 6 hours. Anaerobically cultured. The HAP plate was washed 3 times with distilled water, stained with plaque staining solution for 3 minutes, then washed 3 times with distilled water and allowed to air dry. The HAP surface was measured with a color difference meter, and the Δa value was measured as an indicator of plaque formation.
An average value of three repetitions was obtained, and those having a result of ◯ or more were judged to have a high plaque formation suppressing effect according to the following criteria.
◎: Δa value is 0 or more and less than 15 ○: Δa value is 15 or more and less than 30 Δ: Δa value is 30 or more and less than 45 ×: Δa value is 45 or more

また、フレーバー１〜６の組成は、表４〜９に示す通りである。 About the fragrance | flavor, the fragrance | flavor A-the fragrance | flavor I shown in Table 3 were produced and mix | blended.

The compositions of flavors 1 to 6 are as shown in Tables 4 to 9.

  From the above results, the dentifrice composition (comparative example) that does not satisfy any of the essential requirements of the present invention is inferior to any of the dextranase stability, foaming during dentifrice, and plaque formation inhibitory effect. The compositions of the present invention (Examples) were all good (◯ or more).

[Example 10] Toothpaste (A) dextranase (13,000 units / g) 0.1%
(B) Palm oil fatty acid amidopropyl betaine 0.8
(C) Cetylpyridinium chloride 0.05
Polyoxyethylene (60) hydrogenated castor oil 0.5
Xanthan gum 0.8
Sodium polyacrylate 0.7
Silica 18
70% sorbite solution 40
Saccharin sodium 0.2
Propylene glycol 4
DL-alanine 0.5
Methyl paraoxybenzoate 0.06
Butyl paraoxybenzoate 0.01
Titanium oxide 0.3
Sodium monofluorophosphate 0.7
Fragrance A 1.3
Water remaining
Total 100.0%
(B) / (D) = 16

  The above dentifrice composition was prepared (pH = 7.4, 25 ° C.), and 50 g was filled in a 26 mm diameter laminated tube whose innermost layer was made of linear low density polyethylene. When evaluated in the same manner as in Example 1, the results were dextranase stability (◎), foaming during dentifrice (◎), and plaque formation inhibitory effect (◎).

[Example 11] Toothpaste (A) dextranase (13,000 units / g) 0.2%
(B) Amidopropyl betaine laurate 0.9
(C) Benzalkonium chloride 0.05
Xanthan gum 0.8
Sodium alginate 0.5
N-coconut oil fatty acid acyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine (30% aqueous solution) 0.5
Polyoxyethylene (20) cetyl ether 0.2
Aluminum hydroxide 40
70% sorbite solution 30
Saccharin sodium 0.2
Propylene glycol 3
Fragrance A 1.4
Triclosan 0.1
ε-aminocaproic acid 0.03
Sodium fluoride 0.21
Water remaining
Total 100.0%
(B) / (D) = 18

  The above dentifrice composition was prepared (pH = 7.8, 25 ° C.), and 50 g was filled in a 26 mm diameter laminated tube whose innermost layer was made of linear low density polyethylene. As a result of evaluation in the same manner as described above, dextranase stability (◎), foaming during tooth brushing (◎), and plaque formation inhibitory effect (◎) were obtained.

[Example 12] Toothpaste (A) dextranase (13,000 units / g) 0.1%
(B) Palm oil fatty acid amidopropyl betaine 0.6
(C) Benzalkonium chloride 0.01
(C) Benzethonium chloride 0.01
Polyoxyethylene hydrogenated castor oil 0.8
Lysozyme chloride 0.2
Xanthan gum 1.0
Silica 20
Hydroxyethyl cellulose 1
Polyethylene glycol 4000 0.45
70% sorbit solution 25
Xylitol 5
Isopropyl methylphenol 0.05
Saccharin sodium 0.1
Propylene glycol 3
Fragrance A 1.3
Sodium monofluorophosphate 0.7
Tranexamic acid 0.05
Dipotassium glycyrrhizinate 0.1
Water remaining
Total 100.0%
(B) / (D) = 30

  The above dentifrice composition was prepared (pH = 7.3, 25 ° C.), and 50 g was filled in a 26 mm diameter laminated tube whose innermost layer was made of linear low density polyethylene. When evaluated in the same manner as described above, they were dextranase stability (◎), foaming during tooth brushing (○), and plaque formation inhibitory effect (◎).

[Example 13] Toothpaste (A) dextranase (13,000 units / g) 0.2% by mass
(B) Amidopropyl betaine laurate 0.8
(C) Cetylpyridinium chloride 0.05
Polyoxyethylene (20) hydrogenated castor oil 0.6
Sodium lauryl sulfate 0.4
Xanthan gum 0.8
Sodium alginate 0.4
Aluminum hydroxide 40
Gelatin 0.5
70% sorbit solution 25
Saccharin sodium 0.2
Propylene glycol 3
Sodium monofluorophosphate 0.7
Cetylpyridinium chloride 0.05
ε-aminocaproic acid 0.05
Fragrance A 1.3
Water remaining
Total 100.0%
(B) / (D) = 16

  The above dentifrice composition was prepared (pH = 7.5, 25 ° C.), and 50 g was filled into a 26 mm diameter laminated tube whose innermost layer was made of linear low density polyethylene. When evaluated in the same manner as described above, the results were dextranase stability (◯), foaming during tooth brushing (）), and plaque formation inhibitory effect (○).

Claims (3)

(A) To a dentifrice composition containing an abrasive containing dextranase, (B) 0.5 to 2.0% by mass of fatty acid amidopropyl betaine having an alkyl chain length of 12 to 14 carbon atoms, (C) 0.01-0.1% by mass of one or more cationic fungicides selected from cetylpyridinium chloride, benzalkonium chloride, and benzethonium chloride , and (B) / (C) is 10 by mass ratio . formulated within an amount of 150, and dentifrice composition characterized in that the pH range of 6.5 to 8.5 of the composition at 25 ° C.. The dentifrice composition according to claim 1, wherein the abrasive is at least one selected from a silica-based abrasive and aluminum hydroxide. The dentifrice composition according to claim 1 or 2, which does not contain an anionic surfactant.