JP5228380B2 - Dentifrice composition - Google Patents

Description

  The present invention relates to a dentifrice composition having high stability of dextranase in a preparation, excellent plaque removal effect, little taste after use, and high oral cleaning feeling.

  Dextranase exhibits a caries prevention effect by decomposing plaque, and is therefore blended in the oral composition as an active ingredient.

  On the other hand, surfactants are blended in the oral composition from the viewpoint of usability and usability, and in particular, a large amount of abrasive is blended in the dentifrice composition, ensuring sufficient foamability. Therefore, an anionic surfactant such as sodium lauryl sulfate is blended. However, a dentifrice composition containing many anionic surfactants has a drawback that it is difficult to maintain the temporal stability of the enzyme due to its protein-denaturing action. In a dentifrice composition containing dextranase, However, the problem was the decrease in the stability of dextranase over time.

  For this reason, in a dentifrice composition containing dextranase, a technology for stabilizing the dextranase enzyme is essential in the preparation, and a specific non-anionic surfactant is added for stabilization. Is known to be useful. For example, a carboxylic acid type amphoteric surfactant (Patent Document 1; JP 58-225007 A), polyoxyethylene alkyl ether (Patent Document 2; JP 61-176518 A), glycerin fatty acid ester (Patent Document) 3; Japanese Patent Laid-Open No. 3-181413), and examples in which a fatty acid alkanolamide is blended (Patent Document 4; Japanese Patent Laid-Open No. 2005-187360) are proposed.

  However, in these technical means, the protein denaturation action by an anionic surfactant may be improved to some extent, but when a large amount of nonionic surfactant is added, a unique taste is produced and the oral dispersibility of the preparation is increased. In addition, there is a problem that when a large amount of amphoteric surfactant is blended, there is a tendency that a peculiar bitter taste is caused and the feeling of use tends to deteriorate, which is satisfactory as a dextranase stabilization technique. It wasn't possible.

  Further, in the oral composition containing glucanase and sodium lauryl sulfate, as a method for providing a dentifrice composition capable of ensuring enzyme stability while ensuring a good foaming amount of sodium lauryl sulfate, Polyoxyethylene alkyl ether having an alkyl group having 14 to 18 carbon atoms and 3 to 8 addition moles of ethylene oxide in the agent composition, and polyoxyethylene having 10 to 80 addition moles of ethylene oxide A technique for preliminarily mixing and dispersing hardened castor oil is disclosed (Patent Document 5; JP-A-2005-41787).

  Furthermore, in the oral composition containing glucanase and nonionic surfactant, glucanase and a technique for providing a dentifrice composition that ensures enzyme stability, suppresses irritation by a fragrance, and improves fragrance. A polyoxyethylene alkyl ether having an alkyl group having 14 to 18 carbon atoms and an ethylene oxide addition mole number of 3 to 8, and a polyoxyethylene hydrogenated castor oil having an ethylene oxide addition mole number of 10 to 30 Has been proposed in which a dentifrice composition containing a C7-11 fatty acid triglyceride and a fragrance are contained at a mass ratio of 0.10 to 0.48 (Patent Document 6; JP-A-2005-179290). Publication).

  However, in the proposal to ensure the enzyme stability of the above glucanase, it is difficult to obtain a clean feeling that the oral cavity has a refreshing taste after brushing the teeth, which greatly affects the feeling of use of the dentifrice. It was difficult to maintain the taste and cleanliness after use at the same time.

  On the other hand, sodium hydrogen carbonate is known as a component that maintains a refreshing feeling in the mouth (see Patent Document 7: Japanese Patent Laid-Open No. 11-49654), and it is also known that a high effect of removing tongue coating is obtained ( (Patent Document 8: see JP-A-10-182384).

  However, in the above technical means, it is necessary to add a large amount of sodium hydrogen carbonate in order to maintain a clean feeling such as a refreshing feeling in the oral cavity. In this case, since the pH of the preparation becomes too high, the enzyme is stabilized. It could not be blended and it was difficult to apply this technique to enzyme blend compositions.

  For this reason, in the dentifrice composition which mix | blended dextranase, it was difficult to satisfy | fill both the stabilization of dextranase and the refreshing feeling (cleaning actual feeling) in a mouth satisfactorily. Accordingly, there has been a demand for the development of a dentifrice composition that has high stability of dextranase, exhibits an excellent plaque removal effect, has little taste after brushing, and provides an excellent cleaning feeling.

JP 58-225007 A JP-A 61-176518 JP-A-3-181413 JP 2005-187360 A JP 2005-41787 A JP 2005-179290 A JP 11-49654 A Japanese Patent Laid-Open No. 10-182384

  The present invention has been made in view of the above circumstances, and is a toothpaste with high dextranase stability over time in a preparation, excellent plaque removal effect, little taste after use, and high oral cleaning feeling. An object is to provide an agent composition.

As a result of diligent studies to achieve the above object, the present inventors have found that (A) a dentifrice composition containing 2 to 200 units / g of dextranase is combined with (B) sodium lauryl sulfate in an amount of 0.3. to 2% by weight and, (C) the average number of moles of added ethylene oxide and 0.3 to 3 wt% of 10 to 30 moles of polyoxyethylene hydrogenated castor oil, (D) an alkyl group of carbon number 14 to 18 And 0.3 to 3 % by mass of polyoxyethylene alkyl ether having an average addition mole number of ethylene oxide of 3 to 8 mol, and (E) 0.1 to 1.0% by mass of sodium hydrogen carbonate By blending, the stability of dextranase over time is higher than that of the conventional technology, and excellent aging stability is obtained. In addition, it exhibits an excellent plaque removal effect and is a nonionic surfactant after brushing. Different origin There have almost no good feeling, and then finding a refreshed feeling (cleaning felt) also increases it in the mouth, leading to the completion of the present invention.

  In the present invention, by blending the above components (A) to (E) in combination, as will be apparent from the examples described later, these components act synergistically to make dextranase much more stable. In addition, it has a high plaque removal effect on plaques formed by oral caries-causing bacteria such as Streptococcus sobrinus, and it is excellent in that the non-ionic surfactant-derived taste is hardly felt. In addition to the feeling of use, it is possible to achieve a special effect of increasing the refreshing feeling (cleaning feeling) in the oral cavity after brushing. Such an effect of the present invention is particularly effective for the plaque of dextranase. It is considered that the removal power is improved and the cleaning effect of sodium hydrogen carbonate.

Accordingly, the present invention provides (A) a dentifrice composition containing 2 to 200 units / g of dextranase, (B) 0.3 to 2% by mass of sodium lauryl sulfate, and (C) an average of ethylene oxide. 0.3 to 3 % by mass of polyoxyethylene hydrogenated castor oil having an addition mole number of 10 to 30 moles, (D) having an alkyl group having 14 to 18 carbon atoms, and an average addition mole number of ethylene oxide being 3 A dentifrice composition comprising ˜8 mol of polyoxyethylene alkyl ether in an amount of 0.3 to 3 % by mass and (E) 0.1 to 1.0% by mass of sodium bicarbonate is provided. To do.

  The dentifrice composition of the present invention is stabilized and formulated with dextranase, is excellent in plaque removal effect, has almost no taste after use, has a high cleaning feeling in the oral cavity, and is excellent in usability.

Hereinafter, the present invention will be described in more detail. The dentifrice composition of the present invention is preferably prepared as a toothpaste such as toothpaste and liquid toothpaste, particularly as a toothpaste,
(A) dextranase,
(B) sodium lauryl sulfate,
(C) polyoxyethylene hydrogenated castor oil having an average addition mole number of ethylene oxide of 10 to 30 moles,
(D) A polyoxyethylene alkyl ether having an alkyl group having 14 to 18 carbon atoms and an average addition mole number of ethylene oxide of 3 to 8 moles, and (E) sodium hydrogen carbonate is added to 0.1% of the total composition. ~ 1.0 mass%
It contains.

  (A) As the dextranase, dextranase obtained by a known method from a known dextranase-producing bacterium belonging to the genus Ketomium, Penicillium, Aspergillus, Spicaria, Lactobacillus, Servibrio, etc. is preferable. However, dextranase produced from other microorganisms can also be used, and commercially available products such as those manufactured by Sankyo Co., Ltd. can be used.

The blending amount of dextranase is 2 to 200 units / g (U / g) in the composition , and preferably 10 to 50 units / g. When it is less than 2 units / g, can not be obtained sufficient plaque removing effect, Ru poor cleaning feel after toothbrushing. When more than 200 units / g, that stability is such not sufficient. Here, 1 unit of dextranase is the amount of dextranase that produces free reducing sugar corresponding to 1 μmol of glucose per minute when the reaction is carried out using dextran as a substrate. Usually, when 13000 units / g is used, the blending amount of dextranase is preferably 0.0154 to 1.54% (mass%, the same applies hereinafter) of the entire composition.

(B) As sodium lauryl sulfate, for example, commercially available sodium lauryl sulfate manufactured by Toho Chemical Industry Co., Ltd. and Daiichi Pharmaceutical Co., Ltd. can be used.
The blending amount of sodium lauryl sulfate is preferably 0.3 to 2.0 % of the entire composition, and more preferably in the range of 0.3 to 1.5%. If the blending amount is less than 0.3%, sufficient plaque removal effect and cleaning feeling may not be obtained, and if it exceeds 2.0%, the stability of dextranase may be inferior.

  Next, as (C) polyoxyethylene hydrogenated castor oil, the average added mole number of ethylene oxide is 10 to 30 mol, preferably 20 to 30 in terms of dentifrice stability and dextranase stabilization effect. Use molar ones. When the average added mole number of ethylene oxide is less than 10 moles, a peculiar taste occurs and the taste of the preparation is deteriorated. When the average added mole number exceeds 30, the dextranase stabilization effect is inferior.

  Examples of such polyoxyethylene hydrogenated castor oil include HCO-10, HCO-20, and HCO-30 manufactured by Nikko Chemicals Co., Ltd., and HC-10, HC-20, and HC-30 manufactured by Nihon Emulsion Co., Ltd. , BLAUNON RCW-20 manufactured by Aoki Oil & Fat Co., Ltd.

The blending amount of the polyoxyethylene hydrogenated castor oil is 0.3 to 3 % of the whole composition , and preferably 0.5 to 2%. Amount is you decrease dextranase stability is less than 0.3%. More than 3%, the arising problems with feeling by, for example, of specific off-taste may occur.

As the (D) polyoxyethylene alkyl ether used in the dentifrice composition of the present invention, the following general formula (1)
R—O— (EO) _n H (1)
(In the formula, R is an alkyl group having 14 to 18 carbon atoms, n is 3 to 8, and EO represents ethylene oxide.)
The one shown in is used.

  As the polyoxyethylene alkyl ether, those having an alkyl group having 14 to 18 carbon atoms, preferably 16 to 18 carbon atoms, are used in terms of the stability of dentifrice and the stability of dextranase. When the number of carbon atoms in the alkyl group is less than 14, there may be a problem in use feeling due to the occurrence of a peculiar taste, and when the number of carbon atoms in the alkyl group exceeds 18, the dextranase stability is poor.

  Moreover, the average addition mole number of the ethylene oxide of the said polyoxyethylene alkyl ether is the range of 3-8 mol from the point of stability of a dentifrice, and dextranase stability, Preferably it is the range of 5-8 mol. When the average number of added moles is lower than 3 moles, liquid separation may occur in the preparation, resulting in poor dextranase stability. Moreover, when it exceeds 8 mol, it is inferior to dextranase stability, and a nasty taste may arise after tooth brushing.

  Specific examples of such polyoxyethylene alkyl ether include polyoxyethylene cetyl ether, polyoxyethylene myristyl ether, polyoxyethylene stearyl ether, and the like, and polyoxyethylene stearyl ether is particularly preferable. As such polyoxyethylene alkyl ether, commercially available products can be used. For example, polyoxyethylene (3) cetyl ether (EMALEX 103), polyoxyethylene (5) cetyl ether (EMALEX 105) manufactured by Nippon Emulsion Co., Ltd. , Polyoxyethylene (7) cetyl ether (EMALEX 107), polyoxyethylene (3) stearyl ether (EMALEX 603), polyoxyethylene (5) stearyl ether (EMALEX 605), polyoxyethylene (6) stearyl ether (EMALEX 606), poly Examples thereof include oxyethylene (8) stearyl ether (EMALEX 608). Among these, from the standpoint of stability and usability of dextranase, ethylene oxide having an average addition mole number of 5 to 8 moles and an alkyl chain length of 16 to 18 carbon atoms is particularly preferable.

The blending amount of the polyoxyethylene alkyl ether is 0.3 to 3 % of the entire composition , and particularly preferably 0.5 to 2%. Amount is you decrease dextranase stability is less than 0.3%. More than 3%, and together with tend to use feel is poor, such as taste, inferior Ru in dextranase stability.

(E) As sodium hydrogencarbonate, commercially available products such as those manufactured by Asahi Glass Co., Ltd. and Tosoh Corp. can be used.
The compounding quantity of sodium hydrogencarbonate is 0.1 to 1.0% of the whole composition, Preferably it is 0.1 to 0.6%. If the blending amount is less than 0.1%, a peculiar taste of nonionic surfactant is produced, and the plaque removal effect and cleaning feeling after brushing are inferior. If it exceeds 1.0%, the formulation and dextranase are stable. Sexuality gets worse.

  In the dentifrice composition of the present invention, in addition to the above essential components, other additives can be blended as optional components depending on the dosage form as long as the effects of the present invention are not impaired. For example, when preparing a toothpaste, abrasives, thickeners, binders, surfactants, sweeteners, preservatives, active ingredients, pigments, fragrances, etc. can be blended, these ingredients are mixed with water, It can be produced by a conventional method.

Examples of the abrasive include dicalcium phosphate dihydrate, anhydrous dibasic calcium phosphate, calcium pyrophosphate, aluminum hydroxide, alumina, titanium dioxide, crystalline zirconium silicate, polymethyl methacrylate, insoluble calcium metaphosphate, light carbonate Calcium, heavy calcium carbonate, magnesium carbonate, tribasic magnesium phosphate, zeolite, zirconium silicate, tricalcium phosphate, hydroxyapatite, fluoroapatite, calcium deficient apatite, tricalcium phosphate, tetracalcium phosphate, eighth calcium phosphate, synthetic resin Examples thereof include system abrasives, precipitated silica, silica gel, aluminosilicate, and zirconosilicate. Among these abrasives, an abrasive selected from silica-based abrasives and aluminum hydroxide is preferably used from the viewpoint of the stability of dextranase.
10-50% of the whole composition is suitable for the compounding quantity of these abrasive | polishing agents.

  As the thickening agent, at least one of glycerin, sorbit, propylene glycol, polyethylene glycol having an average molecular weight of 200 to 6000, ethylene glycol, polyvalent alcohol such as reduced starch saccharified product, and the like can be used. Usually, the compounding quantity of these thickeners is 10 to 40% of the whole composition.

  As the binder, organic binders such as sodium alginate, propylene glycol alginate, carboxymethyl cellulose, polyvinyl alcohol, hydroxyethyl cellulose, sodium polyacrylate, xanthan gum, carbopol, guar gum, gelatin, avicel, montmorillonite, kaolin, Examples thereof include inorganic binders such as bentonite. Usually, the compounding quantity of these binders is 0.5 to 3% of the whole composition.

  In addition to the essential components (B) sodium lauryl sulfate, (C) specific polyoxyethylene hydrogenated castor oil, and (D) specific polyoxyethylene alkyl ether as surfactants, the effects of the present invention are hindered. Other surfactants can be added within the range. For example, other anionic surfactants include alkyl sulfates other than sodium lauryl sulfate, lauroyl sarcosine salt, myristyl sarcosine salt, polyoxyethylene alkyl sulfate, N-lauroyl taurine salt, α-olefin sulfonate, and the like. Can be mentioned. Other nonionic surfactants include, for example, alkyl glycosides having 8 to 16 carbon atoms in the alkyl group, sucrose fatty acid esters having 12 to 18 carbon atoms in the fatty acid group such as sucrose mono or dilaurate, and lactose fatty acids. Esters, lactitol fatty acid esters, maltitol fatty acid esters, stearic acid monoglyceride, polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monostearate, lauroyl diethanolamide and other alkyloyl ethanolamides, polyoxyethylene polyoxypropylene glycol, laurin Examples include acid decaglyceryl. Examples of the amphoteric surfactant include alkyldimethylaminoacetic acid betaine, fatty acid amidopropyl betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine and the like.

  These other surfactants as optional components can be blended singly or in combination of two or more, and the blending amount is usually 0.1 to 3% based on the total amount of the composition, (B), (C) and (D) It is preferable to mix | blend in the range from which the total compounding quantity of surfactant of said component and above-mentioned other surfactant becomes 0.9 to 10% of the whole composition.

  Sweeteners include sodium saccharin, aspartame, stevioside, stevia extract, paramethoxycinnamic aldehyde, neohesperidyl dihydrochalcone, perilartin, etc., and preservatives include parabens such as butylparaben and ethylparaben, paraoxybenzoates Nonionic antibacterial agents such as sodium benzoate and triclosan, cationic antibacterial agents such as benzethonium chloride, benzalkonium chloride and cetylpyridinium chloride, essential oil components, and the like can be blended.

  As various active ingredients, in addition to the essential ingredient (A) dextranase, other active ingredients can be added. For example, fluorides such as sodium fluoride, potassium fluoride, stannous fluoride, strontium fluoride, sodium monofluorophosphate, water-soluble phosphate compounds such as potassium salt and sodium salt of orthophosphoric acid, tranexamic acid, Epsilon-aminocaproic acid, allantoinchlorohydroxyaluminum, hinokitiol, ascorbic acid, sodium chloride, dl-tocopherol acetate, dihydrocholesterol, α-bisabolol, triclosan, isopropylmethylphenol, azulene, glycyrrhetin, glycyrrhetinic acid, copper chlorophyllin sodium, chlorophyll, glycero Chelating phosphate compounds such as phosphate, copper compounds such as copper gluconate, aluminum lactate, strontium chloride, potassium nitrate, berberine, hydroxamic acid And derivatives thereof, zeolite, methoxyethylene, maleic anhydride copolymer, polyvinyl pyrrolidone, epidihydrocholesterin, benzethonium chloride, sodium chloride, dihydrocholesterol, trichlorocarbanilide, zinc citrate, sweet cherry extract, buckwheat extract, chamomile, Extracts such as clove, rosemary, ougon, safflower and the like can be mentioned. In addition, the addition amount of these active ingredients can be made into an effective amount in the range which does not prevent the effect of this invention.

  Perfumes are peppermint oil, spearmint oil, anise oil, eucalyptus oil, wintergreen oil, cassia oil, clove oil, thyme oil, sage oil, lemon oil, orange oil, peppermint oil, cardamom oil, coriander oil, mandarin oil, lime Oil, lavender oil, rosemary oil, laurel oil, camomil oil, caraway oil, marjoram oil, bay oil, lemongrass oil, origanum oil, pine needle oil, neroli oil, rose oil, jasmine oil, iris concrete, absolute peppermint , Natural roses such as absolute rose and orange flower, and fragrances processed with these natural fragrances (front reservoir cut, rear reservoir cut, fractional distillation, liquid extraction, essence, powder fragrance, etc.), menthol, carvone , Anethole, cineol, methyl salicylate, syn Mic aldehyde, eugenol, 3-l-menthoxypropane-1.2-diol, thymol, linalool, linalyl acetate, limonene, menthone, menthyl acetate, N-substituted-paramentane-3-carboxamide, pinene, octylaldehyde, citral , Pulegone, carbyl acetate, anisaldehyde, ethyl acetate, ethyl butyrate, allyl cyclohexane propionate, methyl anthranilate, ethyl methyl phenyl glycidate, vanillin, undecalactone, hexanal, ethyl alcohol, propyl alcohol, butanol, Isoamyl alcohol, hexenol, dimethyl sulfide, cycloten, furfural, trimethylpyrazine, ethyl lactate, methyl lactate, ethyl Single-flavored ingredients such as o-acetate, as well as fragrances such as strawberry flavor, apple flavor, banana flavor, pineapple flavor, grape flavor, mango flavor, butter flavor, milk flavor, fruit mix flavor, tropical fruit flavor, and dentifrice composition The well-known perfume material used for can be used, and it is not limited to the perfume of an Example.

  Moreover, although the compounding quantity of a fragrance | flavor is not specifically limited, It is preferable to use said fragrance | flavor raw material 0.000001 to 1% in a formulation composition. Moreover, as a fragrance | flavor for fragrance | flavor using the said fragrance | flavor raw material, it is preferable to use 0.1 to 2.0% in a formulation composition.

  As colorants, Red No. 2, Red No. 3, Red No. 225, Red No. 226, Yellow No. 4, Yellow No. 5, Yellow No. 205, Blue No. 1, Blue No. 2, Blue No. 201, Blue No. 204, Green No. 3 No. is exemplified. In addition, the compounding quantity of these components can be made into a normal quantity in the range which does not prevent the effect of this invention.

  EXAMPLES Hereinafter, although an Example and a comparative example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to the following Example. In the following examples, the blending amount is mass%.

Examples and comparative examples
A dentifrice composition having the composition shown in Tables 1 and 2 was prepared by the following method, and a laminated tube (LDPE55 / PET12 / LDPE20 / white LDPE60 / EMAA20 / AL10 / EMAA30 having an innermost layer made of linear low-density polyethylene and having a diameter of 26 cm). / LDPE20 / LLDPE30, thickness 257 μm (manufactured by Dai Nippon Printing Co., Ltd.)), dextranase stabilization effect, plaque removal effect, no taste after use, and oral cleaning feeling in the following method It was evaluated by. The results are shown in Tables 1 and 2.

(1) Preparation of test dentifrice composition The preparation of the dentifrice composition was prepared by dissolving a water-soluble substance such as sodium saccharin and sorbit in purified water and then dispersing a propylene glycol solution in which a binder such as sodium alginate was dispersed. In addition, sodium hydrogen carbonate, polyoxyethylene hydrogenated castor oil, and polyoxyethylene alkyl ether were added and stirred. Then, the fragrance | flavor, the abrasive | polishing agent, the dextranase, and the sodium lauryl sulfate were added in order, and also it stirred under reduced pressure (40 mmHg), and obtained the dentifrice composition. In addition, the unimixer (FM-SR-25, POWEX CORPORATION) was used for manufacture.

  For the preparation of these dentifrice compositions, dextranase (manufactured by Sankyo Co., Ltd.), sodium lauryl sulfate (manufactured by Toho Chemical Co., Ltd.), polyoxyethylene hydrogenated castor oil (manufactured by Nippon Emulsion Co., Ltd.), poly Oxyethylene stearyl ether (manufactured by Nippon Emulsion Co., Ltd.), polyoxyethylene oleyl ether (manufactured by Nippon Emulsion Co., Ltd.), and sodium hydrogen carbonate (manufactured by Asahi Glass Co., Ltd.) were used. In addition, sodium monofluorophosphate, sodium saccharin, aluminum hydroxide, silicic anhydride, xanthan gum, sodium alginate, sodium polyacrylate, propylene glycol, sorbit, methyl paraoxybenzoate, butyl paraoxybenzoate, and water The product was used. About Sorbit, dentifrice was prepared using the 70% aqueous solution product, and the compounding quantity of 70% aqueous solution was shown in the table | surface.

The abbreviations and names in the layer structure of the laminated tube used are as follows, and the numbers following the abbreviations indicate the thickness (μm) of each layer.
LDPE: Low density polyethylene white LDPE: White low density polyethylene LLDPE: Linear low density polyethylene AL: Aluminum EAA: Ethylene / acrylic acid copolymer resin PET: Polyethylene terephthalate EMAA: Ethylene / methacrylic acid copolymer resin SiOx -PET: Glass-deposited polyethylene terephthalate

(2) Evaluation method of dextranase stabilizing effect The dentifrice composition was filled with 50 g in a 26 mm diameter laminated tube whose innermost layer was made of linear low density polyethylene, stored at 40 ° C. for 1 month, and then dentifrice. 0.6 g of the preparation was suspended in 15 mL of 0.1 M phosphate buffer, and the centrifuged supernatant was used as a test solution. 1 mL of this test solution was added to 2 mL of a 1% dextran solution, reacted in a constant temperature bath at 40 ° C. for exactly 10 minutes, and the amount of reducing sugar produced was measured using the Somogy Nelson method. One unit of dextranase was defined as the amount of dextranase that produces free reducing sugar corresponding to 1 μmol of glucose per minute, and the average of three repetitions was evaluated according to the following criteria.

Evaluation criteria ◎: dextranase remaining rate 95% or more ○: dextranase remaining rate 85% or more and less than 95% △: dextranase remaining rate 75% or more and less than 85% ×: dextranase remaining rate less than 75%

(3) Evaluation method of plaque removal effect Streptococcus sobrinas (Streptococcus. Sobrinus) ATCC 6715 was inoculated and cultured at 37 ° C. for 16 hours, and 50 μL of the culture solution was inoculated into 3 mL of medium containing 1% sucrose. Model plaques were prepared by time-graded culture. 4 mL of the supernatant solution obtained by diluting the dentifrice formulation with purified water was added thereto, immersed in the solution at 37 ° C. for 3 minutes, and then allowed to stand at 37 ° C. for 16 hours in 0.1 M phosphate buffer at pH 6.0. did. Thereafter, the plaque peeled off with purified water was removed by washing. After 4 mL of purified water was added, the remaining plaque was dispersed with ultrasonic waves, and the turbidity of the suspension was measured at OD550 nm. Using a sample treated with purified water instead of the dentifrice preparation supernatant as a control, the plaque removal rate was calculated by the following formula, and the evaluation was repeated and averaged 6 times.
Dental plaque removal rate (%) = (1-sample turbidity / control turbidity) × 100

Evaluation criteria ◎: Plaque removal rate of 40% or more ○: Plaque removal rate of 30% or more and less than 40% △: Plaque removal rate of 20% or more and less than 30% ×: Plaque removal rate of less than 20%

(4) Evaluation method of tastelessness after use It evaluated by the sensory test using 10 expert panelists. About 1g of dentifrice is put on a commercial product toothbrush and brushed for 3 minutes. The taste felt after use is evaluated in the following 4 levels in comparison with the control product (Comparative Example 7). According to the standard, it is shown in the table with ◎, ○, Δ, ×.
Evaluation criteria A: 3.5 points or more and 4.0 points or less ○: 3.0 points or more and less than 3.5 points Δ: 2.0 points or more and less than 3.0 points ×: Less than 2.0 points

No taste after use (Control: Comparative Example 7)
4: There was no off-flavor compared to the control product. 3: There was almost no off-taste compared to the control product. 2: There was an equivalent taste compared to the control product. There was a stronger taste compared to the taste of the control product

(5) Evaluation method of oral cleaning feeling (fresh feeling) It evaluated by the sensory test using 10 expert panelists. About 1 g of dentifrice is put on a commercial product toothbrush and brushed for 3 minutes, and the oral cleaning feeling (freshness) felt after use is evaluated in the following four stages in comparison with the control product (Comparative Example 5). The average score of 10 people is shown in the table with ◎, ○, Δ, × according to the following criteria.
Evaluation criteria A: 3.0 points to 4.0 points ○: 2.0 points to less than 3.0 points Δ: 1.5 points to less than 2.0 points ×: Less than 1.5 points

High level of oral cleaning after use (Control: Comparative Example 5)
4: The cleaning feeling was very high compared to the cleaning feeling of the control product 3: The cleaning feeling was considerably higher than the cleaning feeling of the control product 2: The cleaning feeling was slightly higher than the cleaning feeling of the control product It was high 1: It was the same or less than that of the control product

  From the results of Tables 1 and 2, the dentifrice compositions (Examples 1 to 16) formulated by combining the components (A) to (E) of the present invention have a high dextranase stabilizing effect, and the oral cavity It was confirmed that it was excellent in plaque removal effect against caries-causing bacteria in the inside, there was almost no taste after brushing, a refreshing sensation in the oral cavity was obtained satisfactorily, and a clean feeling was also high.

*; Control

Dextonalase (13000 U / g product); Sankyo Co., Ltd .: Dextranase sodium lauryl sulfate; Toho Chemical Co., Ltd .: Sodium lauryl sulfate polyoxyethylene (5) hydrogenated castor oil;
Nippon Emulsion Co., Ltd .: EMALEX HC-5
Polyoxyethylene (10) hydrogenated castor oil;
Nippon Emulsion Co., Ltd .: EMALEX HC-10
Polyoxyethylene (20) hydrogenated castor oil;
Nippon Emulsion Co., Ltd .: EMALEX HC-20
Polyoxyethylene (30) hydrogenated castor oil;
Nippon Emulsion Co., Ltd .: EMALEX HC-30
Polyoxyethylene (40) hydrogenated castor oil;
Nippon Emulsion Co., Ltd .: EMALEX HC-40
Polyoxyethylene (2) stearyl ether;
Nippon Emulsion Co., Ltd .: EMALEX602
Polyoxyethylene (3) stearyl ether;
Nippon Emulsion Co., Ltd .: EMALEX603
Polyoxyethylene (5) stearyl ether;
Nippon Emulsion Co., Ltd .: EMALEX 605
Polyoxyethylene (8) stearyl ether;
Nippon Emulsion Co., Ltd .: EMALEX608
Polyoxyethylene (11) stearyl ether;
Nippon Emulsion Co., Ltd .: EMALEX611
Polyoxyethylene (5) oleyl ether;
Nippon Emulsion Co., Ltd .: EMALEX505H
Polyoxyethylene (5) cetyl ether;
Nippon Emulsion Co., Ltd .: EMALEX705
Sodium bicarbonate; Asahi Glass Co., Ltd .: Sodium bicarbonate

Claims (2)

(A) To a dentifrice composition containing 2 to 200 units / g of dextranase, (B) 0.3 to 2% by mass of sodium lauryl sulfate, and (C) an average added mole number of ethylene oxide is 10 to 10. 30 to 30 % polyoxyethylene hydrogenated castor oil , 0.3 to 3 % by mass , (D) polyoxygen having an alkyl group having 14 to 18 carbon atoms and an average added mole number of ethylene oxide of 3 to 8 mol A dentifrice composition comprising 0.3 to 3 % by mass of ethylene alkyl ether and 0.1 to 1.0% by mass of (E) sodium hydrogen carbonate. Furthermore, according to claim 1 dentifrice composition according blended with abrasive agent selected from silica-based polishing agents and aluminum hydroxide.