JP4842834B2 - 糸状菌株における遺伝子の発現を排除するか又は低めるための方法 - Google Patents

糸状菌株における遺伝子の発現を排除するか又は低めるための方法 Download PDF

Info

Publication number
JP4842834B2
JP4842834B2 JP2006544043A JP2006544043A JP4842834B2 JP 4842834 B2 JP4842834 B2 JP 4842834B2 JP 2006544043 A JP2006544043 A JP 2006544043A JP 2006544043 A JP2006544043 A JP 2006544043A JP 4842834 B2 JP4842834 B2 JP 4842834B2
Authority
JP
Japan
Prior art keywords
target gene
trichoderma reesei
region
aspergillus
aspergillus niger
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2006544043A
Other languages
English (en)
Japanese (ja)
Other versions
JP2007513632A (ja
JP2007513632A5 (enExample
Inventor
マイユラン，スチンドラ
裕晃 宇田川
ブロディ，ハワード
Original Assignee
ノボザイムス，インコーポレイティド
ノボザイムス アクティーゼルスカブ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ノボザイムス，インコーポレイティド, ノボザイムス アクティーゼルスカブ filed Critical ノボザイムス，インコーポレイティド
Publication of JP2007513632A publication Critical patent/JP2007513632A/ja
Publication of JP2007513632A5 publication Critical patent/JP2007513632A5/ja
Application granted granted Critical
Publication of JP4842834B2 publication Critical patent/JP4842834B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2006544043A 2003-12-09 2004-12-09 糸状菌株における遺伝子の発現を排除するか又は低めるための方法 Expired - Fee Related JP4842834B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US52836703P 2003-12-09 2003-12-09
US60/528,367 2003-12-09
PCT/US2004/041510 WO2005056772A1 (en) 2003-12-09 2004-12-09 Methods for eliminating or reducing the expression of genes in filamentous fungal strains

Publications (3)

Publication Number Publication Date
JP2007513632A JP2007513632A (ja) 2007-05-31
JP2007513632A5 JP2007513632A5 (enExample) 2011-08-25
JP4842834B2 true JP4842834B2 (ja) 2011-12-21

Family

ID=34676840

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2006544043A Expired - Fee Related JP4842834B2 (ja) 2003-12-09 2004-12-09 糸状菌株における遺伝子の発現を排除するか又は低めるための方法

Country Status (6)

Country Link
US (2) US8716023B2 (enExample)
EP (1) EP1702059B1 (enExample)
JP (1) JP4842834B2 (enExample)
CN (2) CN1914311B (enExample)
DK (1) DK1702059T3 (enExample)
WO (1) WO2005056772A1 (enExample)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1702059B1 (en) * 2003-12-09 2012-11-14 Novozymes Inc. Methods for eliminating or reducing the expression of genes in filamentous fungal strains
WO2006070227A2 (en) * 2004-10-04 2006-07-06 Devgen Nv Method for down-regulating gene expression in fungi
EP1799821A2 (en) * 2004-10-15 2007-06-27 DSMIP Assets B.V. Homologous amds genes as selectable marker
BRPI0517462A (pt) 2004-10-21 2008-10-07 Charles L Niblett métodos e materiais para conferir resistência a pestes e patógenos de plantas
PL1850873T3 (pl) 2005-02-08 2019-06-28 Genzyme Corporation Przeciwciała przeciwko tgfbeta
EP1772522A1 (en) * 2005-10-04 2007-04-11 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Control of preservation by biomarkers
GB0602435D0 (en) * 2006-02-07 2006-03-22 F2G Ltd Promoter system
KR100711516B1 (ko) * 2006-02-14 2007-04-27 한양대학교 산학협력단 저전력 및 소면적의 용량 결합형 레벨 시프트 회로
WO2008053019A2 (en) * 2006-11-02 2008-05-08 Dsm Ip Assets B.V. Method for reducing the expression of a gene in a filamentous fungal cell
JP5951924B2 (ja) * 2006-12-21 2016-07-13 ノボザイムス，インコーポレイティド 二次的ｒｎａ干渉による糸状菌株における遺伝子発現の低減又は除去方法
MX2009012844A (es) * 2007-05-31 2009-12-15 Novozymes Inc Composiciones para degradar material celulosico.
US20090246844A1 (en) * 2007-10-26 2009-10-01 Arbor Fuel Inc. Methods for the production of ethanol
WO2010080527A1 (en) 2008-12-19 2010-07-15 Novozymes, Inc. Methods for determining cellulolytic enhancing activity of a polypeptide
BR112012008291A8 (pt) 2009-11-06 2019-05-21 Novozymes Inc composição de enzima, célula hospedeira recombinante, e, métodos de produzir uma composição de enzima, para degradar ou converter um material celulósico, para produzir um produto de fermentação e de fermentar um material celulósico
US8986974B2 (en) 2009-12-18 2015-03-24 Novozymes, Inc. Methods for producing polypeptidies in protease-deficient mutants of trichoderma
MX2013001540A (es) 2010-08-12 2013-04-24 Novozymes Inc Composiciones que comprende un polipeptido que tiene actividad de incremento celulolititico y un compuesto de quinona, y uso de las mismas.
EP2622070B1 (en) 2010-09-30 2016-08-03 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2622068B1 (en) 2010-09-30 2016-07-20 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2012068509A1 (en) 2010-11-18 2012-05-24 Novozymes, Inc. Chimeric polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US8927235B2 (en) 2010-12-06 2015-01-06 Novozymes A/S Methods of hydrolyzing oligomers in hemicellulosic liquor
WO2012083081A1 (en) 2010-12-16 2012-06-21 Novozymes, Inc. Promoters for expressing genes in a fungal cell
CN103998605B (zh) 2011-12-20 2021-08-03 诺维信股份有限公司 纤维二糖水解酶变体和编码它们的多核苷酸
EP3358000A3 (en) 2012-01-05 2018-12-19 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
BR112014028764A2 (pt) 2012-05-18 2017-06-27 Novozymes As mutante isolado, métodos para a obtenção do mutante isolado e de um lactobacillus transformante, e, lactobacillus transformante.
AR091116A1 (es) 2012-05-22 2015-01-14 Bristol Myers Squibb Co Anticuerpos biespecificos y sus metodos de uso
CN104603269A (zh) 2012-08-17 2015-05-06 诺维信公司 用于在丝状真菌菌株中共沉默基因表达的方法及其应用
WO2014068010A1 (en) 2012-10-31 2014-05-08 Novozymes A/S Isopropanol production by bacterial hosts
WO2014076232A2 (en) 2012-11-19 2014-05-22 Novozymes A/S Isopropanol production by recombinant hosts using an hmg-coa intermediate
EP2935609A1 (en) * 2012-12-21 2015-10-28 Novozymes, Inc. Methods for producing heterologous polypeptides in mutants of trichoderma
US20160222367A1 (en) * 2013-09-06 2016-08-04 Council Of Scientific & Industrial Research Enzyme composition and process of preparation thereof
US20170166939A1 (en) 2013-11-26 2017-06-15 Novozymes A/S Enzyme Compositions and Uses Thereof
EP3152315B1 (en) 2014-06-06 2018-08-15 Novozymes A/S Enzyme compositions and uses thereof
DK3268482T3 (da) 2015-03-09 2020-03-09 Novozymes As Fremgangsmåder til indføring af en flerhed af ekspressionskonstruktioner i en eukaryot celle
CN106566870A (zh) * 2016-08-31 2017-04-19 泰山医学院 一种高通量快速筛选真菌拮抗细菌的方法
EP3887391A1 (en) 2018-11-28 2021-10-06 Novozymes A/S Improved filamentous fungal host cells
EP3894565A1 (en) 2018-12-12 2021-10-20 Novozymes A/S Methods for increasing the productivity of a filamentous fungal cell in the production of a polypeptide
EP4093188A1 (en) 2020-01-24 2022-11-30 Novozymes A/S Mutants of a filamentous fungal cell having increased productivity in the production of a polypeptide
CN111909929B (zh) * 2020-08-13 2022-09-27 中国农业科学院北京畜牧兽医研究所 一种靶向获取里氏木霉纤维素酶调控基因的方法
EP4441202A1 (en) 2021-12-01 2024-10-09 Danisco US Inc. Compositions and methods for enhanced protein production in fungal cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001049844A1 (en) * 1999-12-30 2001-07-12 Rutgers, The State University Of New Jersey Compositions and methods for gene silencing
JP2001525680A (ja) * 1997-05-21 2001-12-11 ゼネカ リミテッド 遺伝子のサイレンシング
WO2003050288A1 (en) * 2001-12-12 2003-06-19 Plant Bioscience Limited Methods and means for gene silencing in plants

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1176937C (zh) * 2003-02-21 2004-11-24 复旦大学附属中山医院 一种双链rna及其用途
JP4789208B2 (ja) * 2003-04-09 2011-10-12 バイオデリバリー サイエンシーズ インターナショナル インコーポレイティッド タンパク質発現に向けられた渦巻型組成物
WO2005026356A1 (en) * 2003-09-12 2005-03-24 Commonwealth Scientific And Industrial Research Organisation Modified gene-silencing nucleic acid molecules and uses thereof
EP1702059B1 (en) * 2003-12-09 2012-11-14 Novozymes Inc. Methods for eliminating or reducing the expression of genes in filamentous fungal strains

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001525680A (ja) * 1997-05-21 2001-12-11 ゼネカ リミテッド 遺伝子のサイレンシング
WO2001049844A1 (en) * 1999-12-30 2001-07-12 Rutgers, The State University Of New Jersey Compositions and methods for gene silencing
WO2003050288A1 (en) * 2001-12-12 2003-06-19 Plant Bioscience Limited Methods and means for gene silencing in plants

Also Published As

Publication number Publication date
JP2007513632A (ja) 2007-05-31
US8716023B2 (en) 2014-05-06
CN102174581A (zh) 2011-09-07
CN1914311A (zh) 2007-02-14
CN1914311B (zh) 2011-05-04
EP1702059A4 (en) 2007-02-28
US20140287475A1 (en) 2014-09-25
WO2005056772A1 (en) 2005-06-23
EP1702059B1 (en) 2012-11-14
EP1702059A1 (en) 2006-09-20
US20050158844A1 (en) 2005-07-21
DK1702059T3 (da) 2013-03-04
CN102174581B (zh) 2013-04-17

Similar Documents

Publication Publication Date Title
JP4842834B2 (ja) 糸状菌株における遺伝子の発現を排除するか又は低めるための方法
JP5951924B2 (ja) 二次的ｒｎａ干渉による糸状菌株における遺伝子発現の低減又は除去方法
US9873881B2 (en) Promoter variants for expressing genes in a fungal cell
WO2008053019A2 (en) Method for reducing the expression of a gene in a filamentous fungal cell
CN103154249B (zh) 用于在丝状真菌中改进的蛋白质生产的方法
US9598698B2 (en) Methods for co-silencing expression of genes in filamentous fungal strains and uses thereof
EP3445776B1 (en) Rlma-inactivated filamentous fungal host cell
Brody et al. RNAi-mediated gene silencing of highly expressed genes in the industrial fungi Trichoderma reesei and Aspergillus niger
CN113056552A (zh) 经修饰的丝状真菌宿主细胞

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20071129

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20100810

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20101109

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20101116

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110209

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110308

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20110607

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20110614

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110707

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20110707

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110802

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110808

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20110906

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20111006

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20141014

Year of fee payment: 3

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees