BR112014028764A2 - mutante isolado, métodos para a obtenção do mutante isolado e de um lactobacillus transformante, e, lactobacillus transformante. - Google Patents

mutante isolado, métodos para a obtenção do mutante isolado e de um lactobacillus transformante, e, lactobacillus transformante.

Info

Publication number
BR112014028764A2
BR112014028764A2 BR112014028764A BR112014028764A BR112014028764A2 BR 112014028764 A2 BR112014028764 A2 BR 112014028764A2 BR 112014028764 A BR112014028764 A BR 112014028764A BR 112014028764 A BR112014028764 A BR 112014028764A BR 112014028764 A2 BR112014028764 A2 BR 112014028764A2
Authority
BR
Brazil
Prior art keywords
isolated mutant
transformant
lactobacillus
lactobacillus transformant
obtaining
Prior art date
Application number
BR112014028764A
Other languages
English (en)
Inventor
Christensen Bjarke
Kobmann Brian
Bjarke Olsen Peter
Troels Joergensen Steen
Bak Regueira Torsten
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of BR112014028764A2 publication Critical patent/BR112014028764A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
BR112014028764A 2012-05-18 2013-05-17 mutante isolado, métodos para a obtenção do mutante isolado e de um lactobacillus transformante, e, lactobacillus transformante. BR112014028764A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261648958P 2012-05-18 2012-05-18
PCT/US2013/041588 WO2013173711A1 (en) 2012-05-18 2013-05-17 Bacterial mutants with improved transformation efficiency

Publications (1)

Publication Number Publication Date
BR112014028764A2 true BR112014028764A2 (pt) 2017-06-27

Family

ID=48539407

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014028764A BR112014028764A2 (pt) 2012-05-18 2013-05-17 mutante isolado, métodos para a obtenção do mutante isolado e de um lactobacillus transformante, e, lactobacillus transformante.

Country Status (5)

Country Link
US (1) US9803209B2 (pt)
EP (1) EP2850180A1 (pt)
CN (1) CN104379734B (pt)
BR (1) BR112014028764A2 (pt)
WO (1) WO2013173711A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2850180A1 (en) * 2012-05-18 2015-03-25 Novozymes A/S Bacterial mutants with improved transformation efficiency
WO2018064108A1 (en) * 2016-09-30 2018-04-05 Invista North America S.á.r.l. Enhanced processes and reagents for host engineering
US11168128B2 (en) 2020-02-26 2021-11-09 Vir Biotechnology, Inc. Antibodies against SARS-CoV-2 and methods of using the same

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US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DE69631118T2 (de) 1995-01-23 2004-07-01 Novozymes A/S Dna-integration durch transposition
EP1706457B1 (en) 2003-12-04 2012-02-01 Novozymes A/S Production of 3-hydroxypropionic acid using beta-alanine/pyruvate aminotransferase
CN102174581B (zh) 2003-12-09 2013-04-17 诺维信股份有限公司 消除或减少丝状真菌菌株基因表达的方法
CN101652479A (zh) 2006-12-21 2010-02-17 诺维信股份有限公司 通过移行rna干扰来降低或消除丝状真菌菌株中的基因表达的方法
BRPI0808988A2 (pt) 2007-03-16 2011-11-08 Genomatica Inc micro-organismos e métodos para a produção de 1,4-butanodiol (1,4-bdo)
WO2009151728A2 (en) 2008-03-27 2009-12-17 Genomatica, Inc. Microorganisms for the production of adipic acid and other compounds
CN102066552B (zh) * 2008-04-04 2014-03-12 麻省理工学院 细胞法产生葡糖二酸
JP2011519561A (ja) 2008-05-01 2011-07-14 ジェノマティカ， インコーポレイテッド メタクリル酸の産生のための微生物
KR102302146B1 (ko) 2009-04-30 2021-09-14 게노마티카 인코포레이티드 1,3-부탄다이올 생산 유기체
ES2749423T3 (es) 2009-05-07 2020-03-20 Genomatica Inc Microorganismos y métodos para la biosíntesis de adipato, hexametilendiamina y ácido 6-aminocaproico
US8129169B2 (en) 2009-06-04 2012-03-06 Genomatica, Inc. Microorganisms for the production of 1,4-butanediol and related methods
US8420375B2 (en) 2009-06-10 2013-04-16 Genomatica, Inc. Microorganisms and methods for carbon-efficient biosynthesis of MEK and 2-butanol
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AU2010289725A1 (en) 2009-09-01 2012-04-05 Novozymes, Inc. Methods for improving malic acid production in filamentous fungi
CN105441374A (zh) 2009-10-13 2016-03-30 基因组股份公司 生产1,4-丁二醇、4-羟基丁醛、4-羟基丁酰-coa、腐胺和相关化合物的微生物及其相关方法
MX336229B (es) 2010-05-05 2016-01-08 Genomatica Inc Microorganismos y metodos para la biosintesis de butadieno.
JP2013544083A (ja) 2010-10-29 2013-12-12 ノボザイムス アクティーゼルスカブ 組み換えｎ−プロパノール及びイソプロパノール生産
EP2714889A1 (en) * 2011-05-27 2014-04-09 Novozymes A/S MICROORGANISMS FOR n-PROPANOL PRODUCTION
EP2850180A1 (en) * 2012-05-18 2015-03-25 Novozymes A/S Bacterial mutants with improved transformation efficiency
WO2014102180A1 (en) * 2012-12-27 2014-07-03 Novozymes A/S Propanol production by lactobacillus bacterial hosts

Also Published As

Publication number Publication date
CN104379734A (zh) 2015-02-25
EP2850180A1 (en) 2015-03-25
CN104379734B (zh) 2018-05-15
US20150125959A1 (en) 2015-05-07
US9803209B2 (en) 2017-10-31
WO2013173711A1 (en) 2013-11-21

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according art. 34 industrial property law
B06F Objections, documents and/or translations needed after an examination request according art. 34 industrial property law
B06I Technical and formal requirements: publication cancelled

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B06U Preliminary requirement: requests with searches performed by other patent offices: suspension of the patent application procedure
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements