JP4831932B2 - アミノペプチダーゼ - Google Patents

アミノペプチダーゼ Download PDF

Info

Publication number
JP4831932B2
JP4831932B2 JP2003527095A JP2003527095A JP4831932B2 JP 4831932 B2 JP4831932 B2 JP 4831932B2 JP 2003527095 A JP2003527095 A JP 2003527095A JP 2003527095 A JP2003527095 A JP 2003527095A JP 4831932 B2 JP4831932 B2 JP 4831932B2
Authority
JP
Japan
Prior art keywords
polypeptide
gene
human
aminopeptidase
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2003527095A
Other languages
English (en)
Japanese (ja)
Other versions
JP2005502359A (ja
Inventor
シー． ジョリー，ジョン
Original Assignee
ジェネンテック， インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ジェネンテック， インコーポレイテッド filed Critical ジェネンテック， インコーポレイテッド
Publication of JP2005502359A publication Critical patent/JP2005502359A/ja
Application granted granted Critical
Publication of JP4831932B2 publication Critical patent/JP4831932B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Detergent Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Peptides Or Proteins (AREA)
JP2003527095A 2001-09-13 2002-09-12 アミノペプチダーゼ Expired - Lifetime JP4831932B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32235001P 2001-09-13 2001-09-13
US60/322,350 2001-09-13
PCT/US2002/029015 WO2003023030A2 (en) 2001-09-13 2002-09-12 Aminopeptidase

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2011094093A Division JP5372061B2 (ja) 2001-09-13 2011-04-20 アミノペプチダーゼ

Publications (2)

Publication Number Publication Date
JP2005502359A JP2005502359A (ja) 2005-01-27
JP4831932B2 true JP4831932B2 (ja) 2011-12-07

Family

ID=23254494

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2003527095A Expired - Lifetime JP4831932B2 (ja) 2001-09-13 2002-09-12 アミノペプチダーゼ
JP2011094093A Expired - Lifetime JP5372061B2 (ja) 2001-09-13 2011-04-20 アミノペプチダーゼ

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2011094093A Expired - Lifetime JP5372061B2 (ja) 2001-09-13 2011-04-20 アミノペプチダーゼ

Country Status (21)

Country Link
US (3) US6921659B2 (https=)
EP (1) EP1432793B1 (https=)
JP (2) JP4831932B2 (https=)
KR (2) KR100954641B1 (https=)
CN (1) CN100552023C (https=)
AT (1) ATE440134T1 (https=)
AU (1) AU2002326884B2 (https=)
BR (1) BR0212886A (https=)
CA (1) CA2460309C (https=)
CY (1) CY1109903T1 (https=)
DE (1) DE60233417D1 (https=)
DK (1) DK1432793T3 (https=)
ES (1) ES2329880T3 (https=)
HU (3) HU225346B1 (https=)
IL (2) IL160658A0 (https=)
MX (1) MXPA04002326A (https=)
NZ (2) NZ531516A (https=)
PL (1) PL206286B1 (https=)
PT (1) PT1432793E (https=)
WO (1) WO2003023030A2 (https=)
ZA (1) ZA200401752B (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60233417D1 (en) 2001-09-13 2009-10-01 Genentech Inc Aminopeptidase b2324
BRPI0507233B8 (pt) * 2004-03-11 2021-05-25 Genentech Inc processo para a produção de um polipeptídeo heterólogo em e. coli
US9637746B2 (en) 2008-12-15 2017-05-02 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways
CN105132387A (zh) * 2008-12-22 2015-12-09 绿光生物科学公司 用于制造化合物的组合物和方法
EP2566953B1 (en) 2010-05-07 2019-01-02 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through enzyme relocation
EP2611922A1 (en) 2010-08-31 2013-07-10 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
CN104093848A (zh) 2011-09-09 2014-10-08 绿光生物科学公司 碳青霉烯(carbapenem)的无细胞制备
AU2014306074B2 (en) 2013-08-05 2018-08-30 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
BR112017020690A2 (pt) 2015-03-30 2018-06-26 Greenlight Biosciences Inc produção livre de células de ácido ribonucleico
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CA3020312A1 (en) 2016-04-06 2017-10-12 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN106967658B (zh) * 2017-02-23 2020-09-15 郑州大学 一种提高Fab抗体表达量的方法
KR102894284B1 (ko) 2017-10-11 2025-12-03 그린라이트 바이오사이언시스, 아이엔씨. 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
US12537071B1 (en) 2020-07-22 2026-01-27 David Gordon Bermudes Bacteria having boolean control pathways expressing therapeutic proteins including immunotherapeutic cytotoxins

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62171699A (ja) * 1985-06-04 1987-07-28 Takeda Chem Ind Ltd 蛋白質の製造法
JPH01181796A (ja) * 1988-01-14 1989-07-19 Ajinomoto Co Inc 組換えｄｎａ法を用いたポリペプチドの製造法
JPH03130088A (ja) * 1989-07-21 1991-06-03 Ajinomoto Co Inc 組み替えｄｎａ法によるポリペプチドの製造法
WO1994012636A1 (en) * 1992-11-20 1994-06-09 Genentech, Inc. Method of controlling polypeptide production in bacteria
JPH07203986A (ja) * 1993-12-22 1995-08-08 Eli Lilly & Co 固定化ジクチオステリウムのジペプチジルアミノペプチダーゼを用いるタンパク質の酵素的変換
WO2000066761A2 (en) * 1999-04-30 2000-11-09 Pharmacia Corporation Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986001229A1 (en) 1984-08-16 1986-02-27 Bio-Technology General Corp. Method of removing n-terminal amino acid residues from eucaryotic polypetide analogs and polypeptides produced thereby
US4865974A (en) * 1985-09-20 1989-09-12 Cetus Corporation Bacterial methionine N-terminal peptidase
ATE122391T1 (de) 1987-01-30 1995-05-15 Harvard College Periplasmische protease-mutanten von escherichia coli.
US4946783A (en) * 1987-01-30 1990-08-07 President And Fellows Of Harvard College Periplasmic protease mutants of Escherichia coli
US5508192A (en) * 1990-11-09 1996-04-16 Board Of Regents, The University Of Texas System Bacterial host strains for producing proteolytically sensitive polypeptides
US6127144A (en) * 1993-12-23 2000-10-03 Cangene Corporation Method for expression of proteins in bacterial host cells
US5789199A (en) * 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5639635A (en) * 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
KR100369839B1 (ko) * 1997-02-28 2003-06-12 주식회사 엘지생명과학 바실러스리케니포미스균주에서유래한아미노펩티다제,그의제조방법및이를이용한천연형단백질의제조방법
WO2000034452A1 (en) * 1998-12-07 2000-06-15 Novozymes A/S Glucoamylases with n-terminal extensions
KR100400638B1 (ko) 2000-12-06 2003-10-08 주식회사 엘지생명과학 재조합 인간 성장호르몬 유사체의 분리방법
DE60233417D1 (en) 2001-09-13 2009-10-01 Genentech Inc Aminopeptidase b2324

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62171699A (ja) * 1985-06-04 1987-07-28 Takeda Chem Ind Ltd 蛋白質の製造法
JPH01181796A (ja) * 1988-01-14 1989-07-19 Ajinomoto Co Inc 組換えｄｎａ法を用いたポリペプチドの製造法
JPH03130088A (ja) * 1989-07-21 1991-06-03 Ajinomoto Co Inc 組み替えｄｎａ法によるポリペプチドの製造法
WO1994012636A1 (en) * 1992-11-20 1994-06-09 Genentech, Inc. Method of controlling polypeptide production in bacteria
JPH07203986A (ja) * 1993-12-22 1995-08-08 Eli Lilly & Co 固定化ジクチオステリウムのジペプチジルアミノペプチダーゼを用いるタンパク質の酵素的変換
WO2000066761A2 (en) * 1999-04-30 2000-11-09 Pharmacia Corporation Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase

Also Published As

Publication number Publication date
JP2005502359A (ja) 2005-01-27
KR20090018142A (ko) 2009-02-19
US20080160572A1 (en) 2008-07-03
CN100552023C (zh) 2009-10-21
EP1432793B1 (en) 2009-08-19
KR100954641B1 (ko) 2010-04-27
DK1432793T3 (da) 2009-12-07
US20030124664A1 (en) 2003-07-03
ATE440134T1 (de) 2009-09-15
PL368463A1 (en) 2005-04-04
AU2002326884B2 (en) 2007-10-18
KR100956907B1 (ko) 2010-05-11
NZ544964A (en) 2007-08-31
EP1432793A4 (en) 2006-05-24
ES2329880T3 (es) 2009-12-02
US20060019341A1 (en) 2006-01-26
IL160658A0 (en) 2004-08-31
HU225243B1 (en) 2006-08-28
HU0600310D0 (en) 2006-06-28
CA2460309A1 (en) 2003-03-20
BR0212886A (pt) 2004-10-13
HU225346B1 (en) 2006-10-28
US7229787B2 (en) 2007-06-12
JP2011177189A (ja) 2011-09-15
KR20040041611A (ko) 2004-05-17
NZ531516A (en) 2006-03-31
CN1555412A (zh) 2004-12-15
US7632658B2 (en) 2009-12-15
US6921659B2 (en) 2005-07-26
HUP0401869A2 (hu) 2004-12-28
CY1109903T1 (el) 2014-09-10
WO2003023030A2 (en) 2003-03-20
PL206286B1 (pl) 2010-07-30
IL160658A (en) 2009-05-04
CA2460309C (en) 2012-07-10
ZA200401752B (en) 2005-05-25
WO2003023030A3 (en) 2003-07-10
PT1432793E (pt) 2009-11-24
HU225430B1 (en) 2006-11-28
DE60233417D1 (en) 2009-10-01
HU0600309D0 (en) 2006-06-28
JP5372061B2 (ja) 2013-12-18
MXPA04002326A (es) 2004-06-29
HK1062695A1 (en) 2004-11-19
HUP0401869A3 (en) 2005-06-28
EP1432793A2 (en) 2004-06-30

Similar Documents

Publication Publication Date Title
JP5372061B2 (ja) アミノペプチダーゼ
JP3749257B2 (ja) ジスルフィド結合を有する異種ポリペプチドの細菌による産生方法
EP0856059B1 (en) Methods for production of recombinant plasmids
AU2002326884A1 (en) Aminopeptidase
US5789199A (en) Process for bacterial production of polypeptides
Henderson et al. Artifactual processing of penicillin-binding proteins 7 and 1b by the OmpT protease of Escherichia coli
Inouye et al. Alkaline phosphatase synthesis in a cell-free system using DNA and RNA templates
HK1062695B (en) Aminopeptidase b2324
JPH0773505B2 (ja) 誘発性プロモーターの制御下にある組換ｄｎａの表現法
JPS62151185A (ja) 組換えｄｎａ分子
JPS62155080A (ja) 新規な大腸菌宿主
JPH06339389A (ja) 新規な大腸菌宿主を利用したタンパク又はポリペプチドの製造方法

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20050831

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20081216

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20090316

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20090324

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20090416

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20090501

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20090518

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20090525

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20090616

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20100713

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20101013

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20101020

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20101115

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20101221

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110420

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110531

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20110609

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20110823

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20110920

R150 Certificate of patent or registration of utility model

Ref document number: 4831932

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20140930

Year of fee payment: 3

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term