CY1109903T1 - Αμινοπεπτιδαση β2324 - Google Patents
Αμινοπεπτιδαση β2324Info
- Publication number
- CY1109903T1 CY1109903T1 CY20091101111T CY091101111T CY1109903T1 CY 1109903 T1 CY1109903 T1 CY 1109903T1 CY 20091101111 T CY20091101111 T CY 20091101111T CY 091101111 T CY091101111 T CY 091101111T CY 1109903 T1 CY1109903 T1 CY 1109903T1
- Authority
- CY
- Cyprus
- Prior art keywords
- polypeptide
- aminopeptidase
- sequence
- gene
- native
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 abstract 6
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 6
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 6
- 102000004400 Aminopeptidases Human genes 0.000 abstract 5
- 108090000915 Aminopeptidases Proteins 0.000 abstract 5
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 101100184335 Escherichia coli (strain K12) mnmC gene Proteins 0.000 abstract 3
- 230000001580 bacterial effect Effects 0.000 abstract 2
- 230000002759 chromosomal effect Effects 0.000 abstract 2
- 230000002950 deficient Effects 0.000 abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Detergent Compositions (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Peptides Or Proteins (AREA)
Abstract
Περιγράφεται βακτηριακό κύτταρο αρνητικό κατά Gram το οποίο είναι ανεπαρκές σε χρωμοσωμικό γονίδιο που είναι παρόν σε τέτοιο κύτταρο φυσικού τύπου το οποίο γονίδιο έχει τουλάχιστον 80% ταυτοσημία αλληλουχίας με την φυσική αλληλουχία του γονιδίου yfcK και κωδικοποιεί αμινοπεπτιδάση. Εναλλακτικά, βακτηριακό κύτταρο αρνητικό κατά Gram είναι ανεπαρκές σε χρωμοσωμικό γονίδιο που είναι παρόν σε τέτοιο κύτταρο φυσικού τύπου το οποίο γονίδιο κωδικοποιεί αμινοπεπτιδάση που έχει τουλάχιστον 80% ταυτοσημία αλληλουχίας με την φυσική αλληλουχία αμινοπεπτιδάσης b2324. Ο καθένας από αυτούς τους τύπους κυττάρων, όταν περιλαμβάνει νουκλεϊκό οξύ που κωδικοποιεί ετερόλογο πολυπεπτίδιο, παράγει αμινοτελικό μη διασπασμένο πολυπεπτίδιο όταν καλλιεργείται και το πολυπεπτίδιο ανακτάται, με σχεδόν μηδενική παραγωγή ρυπαίνοντας αμινοτελικού διασπασμένου πολυπεπτιδίου. Αντιστρόφως, παρέχεται μέθοδος διάσπασης αμινοτελικού αμινοξέος από πολυπεπτίδιο που περιλαμβάνει την επαφή του πολυπεπτιδίου με αμινοπεπτιδάση που έχει τουλάχιστον 80% ταυτοσημία αλληλουχίας με την φυσική αλληλουχία αμινοπεπτιδάσης b2324.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32235001P | 2001-09-13 | 2001-09-13 | |
| EP02761635A EP1432793B1 (en) | 2001-09-13 | 2002-09-12 | Aminopeptidase b2324 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CY1109903T1 true CY1109903T1 (el) | 2014-09-10 |
Family
ID=23254494
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CY20091101111T CY1109903T1 (el) | 2001-09-13 | 2009-10-26 | Αμινοπεπτιδαση β2324 |
Country Status (22)
| Country | Link |
|---|---|
| US (3) | US6921659B2 (el) |
| EP (1) | EP1432793B1 (el) |
| JP (2) | JP4831932B2 (el) |
| KR (2) | KR100954641B1 (el) |
| CN (1) | CN100552023C (el) |
| AT (1) | ATE440134T1 (el) |
| AU (1) | AU2002326884B2 (el) |
| BR (1) | BR0212886A (el) |
| CA (1) | CA2460309C (el) |
| CY (1) | CY1109903T1 (el) |
| DE (1) | DE60233417D1 (el) |
| DK (1) | DK1432793T3 (el) |
| ES (1) | ES2329880T3 (el) |
| HK (1) | HK1062695A1 (el) |
| HU (3) | HU225346B1 (el) |
| IL (2) | IL160658A0 (el) |
| MX (1) | MXPA04002326A (el) |
| NZ (2) | NZ531516A (el) |
| PL (1) | PL206286B1 (el) |
| PT (1) | PT1432793E (el) |
| WO (1) | WO2003023030A2 (el) |
| ZA (1) | ZA200401752B (el) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL206286B1 (pl) | 2001-09-13 | 2010-07-30 | Genentech Incgenentech Inc | Komórka Gram-ujemnej bakterii, sposób wytwarzania heterologicznego peptydu, sposób zapobiegania odcinaniu N-końcowego aminokwasu, sposób odcinania N-końcowego aminokwasu i sposób wytwarzania skróconego polipeptydu |
| PL1733037T3 (pl) | 2004-03-11 | 2015-04-30 | Genentech Inc | Sposób wytwarzania polipeptydów |
| US9637746B2 (en) | 2008-12-15 | 2017-05-02 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways |
| CN105132387A (zh) * | 2008-12-22 | 2015-12-09 | 绿光生物科学公司 | 用于制造化合物的组合物和方法 |
| EP2566953B1 (en) | 2010-05-07 | 2019-01-02 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through enzyme relocation |
| WO2012030980A1 (en) | 2010-08-31 | 2012-03-08 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through protease manipulation |
| EP2753702B1 (en) | 2011-09-09 | 2021-12-15 | GreenLight Biosciences, Inc. | Cell-free preparation of carbapenems |
| DK3030671T3 (en) | 2013-08-05 | 2019-01-21 | Greenlight Biosciences Inc | MODIFIED PROTEINS WITH A PROTEASE CLEANING PLACE |
| US9616114B1 (en) | 2014-09-18 | 2017-04-11 | David Gordon Bermudes | Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity |
| KR20230048162A (ko) | 2015-03-30 | 2023-04-10 | 그린라이트 바이오사이언시스, 아이엔씨. | 리보핵산의 무세포 생산 |
| AU2017246458B2 (en) | 2016-04-06 | 2022-08-11 | Greenlight Biosciences, Inc. | Cell-free production of ribonucleic acid |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| CN106967658B (zh) * | 2017-02-23 | 2020-09-15 | 郑州大学 | 一种提高Fab抗体表达量的方法 |
| IL273887B (en) | 2017-10-11 | 2022-09-01 | Greenlight Biosciences Inc | Methods and preparations for the production of nucleoside triphosphate and ribonucleic acid |
| US11471497B1 (en) | 2019-03-13 | 2022-10-18 | David Gordon Bermudes | Copper chelation therapeutics |
| US10973908B1 (en) | 2020-05-14 | 2021-04-13 | David Gordon Bermudes | Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE122004000009I1 (de) * | 1984-08-16 | 2004-11-18 | Savient Pharmaceuticals Inc | Methode zur Herstellung von menschlichen Wachstumshormonen. |
| WO1986007380A1 (en) * | 1985-06-04 | 1986-12-18 | Takeda Chemical Industries, Ltd. | Process for preparing polypeptide |
| US4865974A (en) * | 1985-09-20 | 1989-09-12 | Cetus Corporation | Bacterial methionine N-terminal peptidase |
| US4946783A (en) | 1987-01-30 | 1990-08-07 | President And Fellows Of Harvard College | Periplasmic protease mutants of Escherichia coli |
| EP0300035B1 (en) | 1987-01-30 | 1995-05-10 | The President And Fellows Of Harvard College | Periplasmic protease mutants of escherichia coli |
| JPH01181796A (ja) * | 1988-01-14 | 1989-07-19 | Ajinomoto Co Inc | 組換えｄｎａ法を用いたポリペプチドの製造法 |
| JP2844870B2 (ja) * | 1989-07-21 | 1999-01-13 | 味の素株式会社 | 組み替えｄｎａ法によるポリペプチドの製造法 |
| US5508192A (en) | 1990-11-09 | 1996-04-16 | Board Of Regents, The University Of Texas System | Bacterial host strains for producing proteolytically sensitive polypeptides |
| US5304472A (en) | 1992-11-20 | 1994-04-19 | Genentech, Inc. | Method of controlling polypeptide production in bacterial cells |
| US5573923A (en) * | 1993-12-22 | 1996-11-12 | Eli Lilly And Company | Method for removing N-terminal dipeptides from precursor polypeptides with immobilized dipeptidylaminopeptidase from dictyostelium discoideum |
| US6127144A (en) * | 1993-12-23 | 2000-10-03 | Cangene Corporation | Method for expression of proteins in bacterial host cells |
| US5789199A (en) | 1994-11-03 | 1998-08-04 | Genentech, Inc. | Process for bacterial production of polypeptides |
| US5639635A (en) | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
| KR100369839B1 (ko) | 1997-02-28 | 2003-06-12 | 주식회사 엘지생명과학 | 바실러스리케니포미스균주에서유래한아미노펩티다제,그의제조방법및이를이용한천연형단백질의제조방법 |
| CA2352046A1 (en) * | 1998-12-07 | 2000-06-15 | Novozymes A/S | Glucoamylases with n-terminal extensions |
| KR20020011399A (ko) * | 1999-04-30 | 2002-02-08 | 파마시아 코포레이션 | 에어로모나스 아미노펩티다제로 ν-말단 알라닌 잔기를폴리펩티드로부터 제거하는 방법 |
| KR100400638B1 (ko) | 2000-12-06 | 2003-10-08 | 주식회사 엘지생명과학 | 재조합 인간 성장호르몬 유사체의 분리방법 |
| PL206286B1 (pl) | 2001-09-13 | 2010-07-30 | Genentech Incgenentech Inc | Komórka Gram-ujemnej bakterii, sposób wytwarzania heterologicznego peptydu, sposób zapobiegania odcinaniu N-końcowego aminokwasu, sposób odcinania N-końcowego aminokwasu i sposób wytwarzania skróconego polipeptydu |
-
2002
- 2002-09-12 PL PL368463A patent/PL206286B1/pl unknown
- 2002-09-12 NZ NZ531516A patent/NZ531516A/xx not_active IP Right Cessation
- 2002-09-12 JP JP2003527095A patent/JP4831932B2/ja not_active Expired - Lifetime
- 2002-09-12 CA CA2460309A patent/CA2460309C/en not_active Expired - Lifetime
- 2002-09-12 HU HU0600310A patent/HU225346B1/hu unknown
- 2002-09-12 HU HU0401869A patent/HU225243B1/hu unknown
- 2002-09-12 WO PCT/US2002/029015 patent/WO2003023030A2/en active Application Filing
- 2002-09-12 AT AT02761635T patent/ATE440134T1/de active
- 2002-09-12 KR KR1020047003708A patent/KR100954641B1/ko active IP Right Grant
- 2002-09-12 BR BR0212886-1A patent/BR0212886A/pt not_active Application Discontinuation
- 2002-09-12 DK DK02761635T patent/DK1432793T3/da active
- 2002-09-12 US US10/243,789 patent/US6921659B2/en not_active Expired - Lifetime
- 2002-09-12 HU HU0600309A patent/HU225430B1/hu unknown
- 2002-09-12 CN CNB028179846A patent/CN100552023C/zh not_active Expired - Lifetime
- 2002-09-12 KR KR1020087030860A patent/KR100956907B1/ko active IP Right Grant
- 2002-09-12 ES ES02761635T patent/ES2329880T3/es not_active Expired - Lifetime
- 2002-09-12 PT PT02761635T patent/PT1432793E/pt unknown
- 2002-09-12 EP EP02761635A patent/EP1432793B1/en not_active Expired - Lifetime
- 2002-09-12 DE DE60233417T patent/DE60233417D1/de not_active Expired - Lifetime
- 2002-09-12 AU AU2002326884A patent/AU2002326884B2/en not_active Expired
- 2002-09-12 MX MXPA04002326A patent/MXPA04002326A/es active IP Right Grant
- 2002-09-12 IL IL16065802A patent/IL160658A0/xx unknown
- 2002-09-12 NZ NZ544964A patent/NZ544964A/en not_active IP Right Cessation
-
2004
- 2004-03-01 IL IL160658A patent/IL160658A/en unknown
- 2004-03-03 ZA ZA2004/01752A patent/ZA200401752B/en unknown
- 2004-07-15 HK HK04105179.8A patent/HK1062695A1/xx not_active IP Right Cessation
-
2005
- 2005-05-16 US US11/131,035 patent/US7229787B2/en not_active Expired - Lifetime
-
2007
- 2007-04-27 US US11/741,510 patent/US7632658B2/en not_active Expired - Lifetime
-
2009
- 2009-10-26 CY CY20091101111T patent/CY1109903T1/el unknown
-
2011
- 2011-04-20 JP JP2011094093A patent/JP5372061B2/ja not_active Expired - Lifetime
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CY1109903T1 (el) | Αμινοπεπτιδαση β2324 | |
| ATE454393T1 (de) | Egvii-endoglucanase und dafür codierende nukleinsäuren | |
| BR9813608B1 (pt) | célula hospedeira de bacillus transgênica, e, processo para produzir um polipeptìdeo tendo atividade aminopeptidase. | |
| ATE352626T1 (de) | Polypeptide mit galaktoseoxidaseaktivität und dafür kodierende nukleisäuren | |
| ATE389666T1 (de) | Hohe expressionsrate von proteinen | |
| ATE421994T1 (de) | Genexpressionsmethode | |
| AU6020998A (en) | Methods for production of proteins | |
| AR016216A1 (es) | Fragmento aislado de acidos nucleicos que codifica un polipeptido de por lo menos 40 aminoacidos, que tiene al menos un 80% de identidad, basado en elmetodo clustal de alineamiento, con una enzima de la biosintesis de carotenoides en semillas y plantas, gen quimerico, celula huesped transformada, po | |
| DE69930955D1 (de) | Polypeptide mit lysophospholipaseaktivität und nukleinesäuren die sie kodieren | |
| AU2002314738A1 (en) | Methods for the production of products in host cells | |
| ATE402997T1 (de) | Mutierte penicillin g acylasen | |
| ATE315638T1 (de) | Oxalacetat-hydrolase defiziente fungale wirtszellen | |
| ES2196019T3 (es) | Proteasa fungica. | |
| DK0574347T3 (da) | Hidtil ukendt svampeprotease | |
| AU7843101A (en) | Furin polypeptides with improved characteristics | |
| EP1757684A3 (en) | A 2,6-beta-D-fructan hydrolase enzyme and processes for using the enzyme | |
| KR930013111A (ko) | 바실러스의 파라-니트로벤질 에스테라제를 암호화하는 재조합 dna 화합물 및 발현벡터 | |
| HUP0500349A2 (hu) | Eljárás polipeptidek előállítására | |
| WO2023114831A3 (en) | Improvement of dnase1l3 serum half-life | |
| BR9907767A (pt) | Fragmento de ácido nucléico isolado codificador de toda ou uma parte substancial de uma sintetase de ciclopropano, gene quimérico, célula hospedeira transformada, polipeptìdeo de sintetase de ciclopropano, método de alteração do nìvel de expressão de uma sintetase de ciclopropano em uma célula hospedeira, método de obtenção de um fragmento de ácido nucléico codificador de toda ou uma parte substancial de uma sequência de aminoácidos codificadora de uma sintetase de ciclopropano e produto | |
| CY1116010T1 (el) | Διαδικασια για παραγωγη πολυπεπτιδιων |