JP4831697B2 - 精製方法 - Google Patents

精製方法 Download PDF

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Publication number
JP4831697B2
JP4831697B2 JP2007506111A JP2007506111A JP4831697B2 JP 4831697 B2 JP4831697 B2 JP 4831697B2 JP 2007506111 A JP2007506111 A JP 2007506111A JP 2007506111 A JP2007506111 A JP 2007506111A JP 4831697 B2 JP4831697 B2 JP 4831697B2
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JP
Japan
Prior art keywords
matrix
peg
chromatography
ion exchange
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2007506111A
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English (en)
Japanese (ja)
Other versions
JP2007530971A5 (enExample
JP2007530971A (ja
Inventor
ヴァン・アルスティン，ジェイムズ
ハウッシュマンド，ハミッド
ルングロフ，アンダース
オバーグ，パー−ミカエル
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Bioscience AB
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Bioscience AB, GE Healthcare Bio Sciences Corp filed Critical Amersham Bioscience AB
Publication of JP2007530971A publication Critical patent/JP2007530971A/ja
Publication of JP2007530971A5 publication Critical patent/JP2007530971A5/ja
Application granted granted Critical
Publication of JP4831697B2 publication Critical patent/JP4831697B2/ja
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Expired - Lifetime legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Polyethers (AREA)
JP2007506111A 2004-04-02 2005-03-31 精製方法 Expired - Lifetime JP4831697B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0400886A SE0400886D0 (sv) 2004-04-02 2004-04-02 Process of purification
SE0400886-8 2004-04-02
PCT/SE2005/000467 WO2005094960A1 (en) 2004-04-02 2005-03-31 A method for chromatographic purification

Publications (3)

Publication Number Publication Date
JP2007530971A JP2007530971A (ja) 2007-11-01
JP2007530971A5 JP2007530971A5 (enExample) 2008-05-15
JP4831697B2 true JP4831697B2 (ja) 2011-12-07

Family

ID=32173667

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007506111A Expired - Lifetime JP4831697B2 (ja) 2004-04-02 2005-03-31 精製方法

Country Status (9)

Country Link
US (1) US20070213513A1 (enExample)
EP (1) EP1729867B1 (enExample)
JP (1) JP4831697B2 (enExample)
AT (1) ATE485880T1 (enExample)
AU (1) AU2005228834A1 (enExample)
CA (1) CA2560012A1 (enExample)
DE (1) DE602005024373D1 (enExample)
SE (1) SE0400886D0 (enExample)
WO (1) WO2005094960A1 (enExample)

Families Citing this family (24)

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Publication number Priority date Publication date Assignee Title
CA2842964A1 (en) 2006-09-13 2008-03-20 Abbvie Inc. Cell culture improvements
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
US8268915B2 (en) * 2007-06-19 2012-09-18 Ge Healthcare Bio-Sciences Ab Polymer two phase system and use thereof
WO2009090056A2 (en) 2008-01-18 2009-07-23 F. Hoffmann-La Roche Ag Purification of not-glycosylated polypeptides
US8372286B2 (en) * 2008-02-05 2013-02-12 Ge Healthcare Bio-Sciences Ab Method for production of separation media
FR2930780B1 (fr) * 2008-04-30 2010-08-20 Moet & Chandon Procede de degorgement de vins mousseux
JP2011522055A (ja) * 2008-06-03 2011-07-28 パトリス リミテッド 抗体の精製のためのプロセス
AU2009307737B2 (en) 2008-10-20 2015-07-23 Abbvie Inc. Viral inactivation during purification of antibodies
NZ592095A (en) 2008-10-20 2013-01-25 Abbott Lab Isolation and purification of il-12 and tnf-alpha antibodies using protein a affinity chromatography
EP2686334B2 (en) 2011-03-16 2020-07-01 F.Hoffmann-La Roche Ag Ion exchange chromatography with improved selectivity for the separation of polypeptide monomers, aggregates and fragments by modulation of the mobile phase
US9624261B2 (en) 2011-03-29 2017-04-18 Glaxosmithkline Llc Buffer system for protein purification
CN103717284B (zh) * 2011-06-08 2016-02-24 新加坡科技研究局 通过约束共水合色谱纯化生物制品
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
CN105555795A (zh) * 2013-09-17 2016-05-04 株式会社钟化 新抗体纯化方法和由该方法得到的抗体、以及使用了阳离子交换基团的新抗体纯化法和由该方法得到的抗体
TW201628649A (zh) 2014-10-09 2016-08-16 再生元醫藥公司 減少醫藥調配物中微可見顆粒之方法
SG11201900201YA (en) 2016-08-16 2019-02-27 Regeneron Pharma Methods for quantitating individual antibodies from a mixture
AU2017350807B2 (en) 2016-10-25 2022-07-07 Regeneron Pharmaceuticals, Inc. Methods and systems for chromatography data analysis
CN118416215A (zh) 2017-09-19 2024-08-02 里珍纳龙药品有限公司 减少粒子形成的方法以及由其形成的组合物
CN108152104B (zh) * 2017-12-25 2020-08-11 上海微谱化工技术服务有限公司 超支化水性聚氨酯中二氧化硅填料的分离及定量方法
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
US11420916B2 (en) * 2018-06-08 2022-08-23 Board Of Regents, The University Of Texas System Systems and methods for separation of olefins from mixtures that contain reducing agents
TWI853823B (zh) 2018-07-02 2024-09-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
KR20220078634A (ko) * 2019-10-04 2022-06-10 메르크 파텐트 게엠베하 단백질 a 친화성 크로마토그래피에서 모노클로날 항체의 용리

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002036245A1 (en) * 2000-10-30 2002-05-10 Gradipore Limited Integrated separation methods
US20040030107A1 (en) * 2002-08-06 2004-02-12 Min Wan Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol

Family Cites Families (13)

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Publication number Priority date Publication date Assignee Title
US3869436A (en) * 1971-06-01 1975-03-04 Statens Bakteriologiska Lab Method for fractionating plasma proteins using peg and ion-exchangers
DE3483620D1 (de) * 1983-03-11 1991-01-03 Fujirebio Kk Verfahren zur bestimmung von liganden.
US4683294A (en) * 1985-04-03 1987-07-28 Smith Kline Rit, S.A. Process for the extraction and purification of proteins from culture media producing them
DE3789413T2 (de) * 1986-10-03 1994-07-28 Ciba Geigy Ag Lymphokin-ähnliche Peptide.
US5151358A (en) * 1989-06-13 1992-09-29 Genencor International, Inc. Processes for the recovery of naturally produced chymosin
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
US5443953A (en) * 1993-12-08 1995-08-22 Immunomedics, Inc. Preparation and use of immunoconjugates
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
SE9503925D0 (sv) * 1995-11-07 1995-11-07 Pharmacia Biotech Ab Separationsmedium för IgG
US20020006405A1 (en) * 1996-10-22 2002-01-17 Masaki Kitajima Sepsis remedy comprising anti-il-8 antibody as active ingredient
US7514078B2 (en) * 2001-06-01 2009-04-07 Cornell Research Foundation, Inc. Methods of treating prostate cancer with anti-prostate specific membrane antigen antibodies
US20060275766A1 (en) * 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002036245A1 (en) * 2000-10-30 2002-05-10 Gradipore Limited Integrated separation methods
US20040030107A1 (en) * 2002-08-06 2004-02-12 Min Wan Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol

Also Published As

Publication number Publication date
EP1729867A1 (en) 2006-12-13
SE0400886D0 (sv) 2004-04-02
DE602005024373D1 (de) 2010-12-09
EP1729867B1 (en) 2010-10-27
CA2560012A1 (en) 2005-10-13
ATE485880T1 (de) 2010-11-15
WO2005094960A1 (en) 2005-10-13
AU2005228834A1 (en) 2005-10-13
US20070213513A1 (en) 2007-09-13
JP2007530971A (ja) 2007-11-01

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