ATE485880T1 - Verfahren zur chromatographischen reinigung - Google Patents
Verfahren zur chromatographischen reinigungInfo
- Publication number
- ATE485880T1 ATE485880T1 AT05722292T AT05722292T ATE485880T1 AT E485880 T1 ATE485880 T1 AT E485880T1 AT 05722292 T AT05722292 T AT 05722292T AT 05722292 T AT05722292 T AT 05722292T AT E485880 T1 ATE485880 T1 AT E485880T1
- Authority
- AT
- Austria
- Prior art keywords
- chromatography matrix
- target compound
- purification method
- chromatographic purification
- ionic polyether
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000011097 chromatography purification Methods 0.000 title 1
- 239000012504 chromatography matrix Substances 0.000 abstract 3
- 239000004721 Polyphenylene oxide Substances 0.000 abstract 2
- 238000011210 chromatographic step Methods 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- -1 poly(ethylene glycol) Polymers 0.000 abstract 2
- 229920000570 polyether Polymers 0.000 abstract 2
- 229920001223 polyethylene glycol Polymers 0.000 abstract 2
- 238000001042 affinity chromatography Methods 0.000 abstract 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Peptides Or Proteins (AREA)
- Polyethers (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0400886A SE0400886D0 (sv) | 2004-04-02 | 2004-04-02 | Process of purification |
| PCT/SE2005/000467 WO2005094960A1 (en) | 2004-04-02 | 2005-03-31 | A method for chromatographic purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE485880T1 true ATE485880T1 (de) | 2010-11-15 |
Family
ID=32173667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05722292T ATE485880T1 (de) | 2004-04-02 | 2005-03-31 | Verfahren zur chromatographischen reinigung |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20070213513A1 (enExample) |
| EP (1) | EP1729867B1 (enExample) |
| JP (1) | JP4831697B2 (enExample) |
| AT (1) | ATE485880T1 (enExample) |
| AU (1) | AU2005228834A1 (enExample) |
| CA (1) | CA2560012A1 (enExample) |
| DE (1) | DE602005024373D1 (enExample) |
| SE (1) | SE0400886D0 (enExample) |
| WO (1) | WO2005094960A1 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| EP2500413A1 (en) | 2006-09-13 | 2012-09-19 | Abbott Laboratories | Cell culture improvements |
| WO2008156410A1 (en) * | 2007-06-19 | 2008-12-24 | Ge Healthcare Bio-Sciences Ab | Polymer two phase system and use thereof |
| US20110034672A1 (en) * | 2008-01-18 | 2011-02-10 | Roberto Falkenstein | Purification of not-glycosylated polypeptides |
| JP5596560B2 (ja) * | 2008-02-05 | 2014-09-24 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | 分離媒体の製造方法 |
| FR2930780B1 (fr) | 2008-04-30 | 2010-08-20 | Moet & Chandon | Procede de degorgement de vins mousseux |
| CA2726823A1 (en) * | 2008-06-03 | 2009-12-10 | Patrys Limited | Process for purification of antibodies |
| SG10201702951RA (en) | 2008-10-20 | 2017-06-29 | Abbvie Inc | Viral inactivation during purification of antibodies |
| JP5808249B2 (ja) | 2008-10-20 | 2015-11-10 | アッヴィ・インコーポレイテッド | プロテインａアフィニティークロマトグラフィーを用いる抗体の単離および精製 |
| ES2865449T3 (es) | 2011-03-16 | 2021-10-15 | Hoffmann La Roche | Cromatografía de intercambio iónico con selectividad mejorada para la separación de monómeros, agregados y fragmentos polipeptídicos por modulación de la fase móvil |
| WO2012135415A1 (en) | 2011-03-29 | 2012-10-04 | Glaxosmithkline Llc | Buffer system for protein purification |
| SG195306A1 (en) * | 2011-06-08 | 2013-12-30 | Agency Science Tech & Res | Purification of biological products by constrained cohydration chromatography |
| EP2682168A1 (en) | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
| US10023608B1 (en) | 2013-03-13 | 2018-07-17 | Amgen Inc. | Protein purification methods to remove impurities |
| US10519195B2 (en) * | 2013-09-17 | 2019-12-31 | Kaneka Corporation | Antibody purification method, antibody obtained therefrom, novel antibody purification method using cation exchanger, and antibody obtained therefrom |
| TW201628649A (zh) | 2014-10-09 | 2016-08-16 | 再生元醫藥公司 | 減少醫藥調配物中微可見顆粒之方法 |
| IL264631B2 (en) | 2016-08-16 | 2024-05-01 | Regeneron Pharma | A method for quantifying individual antibodies from a mixture |
| EP4071469B1 (en) | 2016-10-25 | 2025-04-09 | Regeneron Pharmaceuticals, Inc. | Methods for chromatography data analysis |
| WO2019060062A1 (en) | 2017-09-19 | 2019-03-28 | Regeneron Pharmaceuticals Inc. | METHODS OF REDUCING PARTICLE FORMATION AND COMPOSITIONS FORMED THEREFROM |
| CN108152104B (zh) * | 2017-12-25 | 2020-08-11 | 上海微谱化工技术服务有限公司 | 超支化水性聚氨酯中二氧化硅填料的分离及定量方法 |
| CN119881293A (zh) | 2018-03-21 | 2025-04-25 | 沃特世科技公司 | 基于非抗体高亲和力的样品制备、吸附剂、装置和方法 |
| WO2019237100A1 (en) * | 2018-06-08 | 2019-12-12 | Board Of Regents, The University Of Texas System | Systems and methods for separation of olefins from mixtures that contain reducing agents |
| TWI853823B (zh) | 2018-07-02 | 2024-09-01 | 美商里珍納龍藥品有限公司 | 自混合物製備多肽之系統及方法 |
| CA3156648A1 (en) * | 2019-10-04 | 2021-04-08 | Merck Patent Gmbh | ELUTION OF MONOCLONAL ANTIBODIES IN PROTEIN A AFFINITY CHROMATOGRAPHY |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3869436A (en) * | 1971-06-01 | 1975-03-04 | Statens Bakteriologiska Lab | Method for fractionating plasma proteins using peg and ion-exchangers |
| EP0119767B1 (en) * | 1983-03-11 | 1990-11-22 | FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC. | Method of measuring ligands |
| US4683294A (en) * | 1985-04-03 | 1987-07-28 | Smith Kline Rit, S.A. | Process for the extraction and purification of proteins from culture media producing them |
| ES2052602T3 (es) * | 1986-10-03 | 1994-07-16 | Ciba Geigy Ag | Nuevos peptidos afines a las linfocinas. |
| US5151358A (en) * | 1989-06-13 | 1992-09-29 | Genencor International, Inc. | Processes for the recovery of naturally produced chymosin |
| DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
| US5443953A (en) * | 1993-12-08 | 1995-08-22 | Immunomedics, Inc. | Preparation and use of immunoconjugates |
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| US5429746A (en) * | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| SE9503925D0 (sv) * | 1995-11-07 | 1995-11-07 | Pharmacia Biotech Ab | Separationsmedium för IgG |
| CA2268085A1 (en) * | 1996-10-22 | 1998-04-30 | Go Wakabayashi | Sepsis remedy comprising anti-il-8 antibody as active ingredient |
| AUPR110100A0 (en) * | 2000-10-30 | 2000-11-23 | Life Therapeutics Limited | Improved separation of macromolecules |
| US7514078B2 (en) * | 2001-06-01 | 2009-04-07 | Cornell Research Foundation, Inc. | Methods of treating prostate cancer with anti-prostate specific membrane antigen antibodies |
| US7998705B2 (en) * | 2002-08-06 | 2011-08-16 | FUJIFILM Diosynth Biotechnologies U.S.A., Inc | Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol |
| US20060275766A1 (en) * | 2003-01-07 | 2006-12-07 | Haurum John S | Method for manufacturing recombinant polyclonal proteins |
-
2004
- 2004-04-02 SE SE0400886A patent/SE0400886D0/xx unknown
-
2005
- 2005-03-31 JP JP2007506111A patent/JP4831697B2/ja not_active Expired - Lifetime
- 2005-03-31 US US10/594,187 patent/US20070213513A1/en not_active Abandoned
- 2005-03-31 WO PCT/SE2005/000467 patent/WO2005094960A1/en not_active Ceased
- 2005-03-31 DE DE602005024373T patent/DE602005024373D1/de not_active Expired - Lifetime
- 2005-03-31 AT AT05722292T patent/ATE485880T1/de not_active IP Right Cessation
- 2005-03-31 AU AU2005228834A patent/AU2005228834A1/en not_active Abandoned
- 2005-03-31 CA CA002560012A patent/CA2560012A1/en not_active Abandoned
- 2005-03-31 EP EP05722292A patent/EP1729867B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP2007530971A (ja) | 2007-11-01 |
| EP1729867A1 (en) | 2006-12-13 |
| WO2005094960A1 (en) | 2005-10-13 |
| US20070213513A1 (en) | 2007-09-13 |
| EP1729867B1 (en) | 2010-10-27 |
| JP4831697B2 (ja) | 2011-12-07 |
| CA2560012A1 (en) | 2005-10-13 |
| AU2005228834A1 (en) | 2005-10-13 |
| SE0400886D0 (sv) | 2004-04-02 |
| DE602005024373D1 (de) | 2010-12-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |