JP4781999B2 - 癌細胞移植動物の製造方法 - Google Patents
癌細胞移植動物の製造方法 Download PDFInfo
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- JP4781999B2 JP4781999B2 JP2006510753A JP2006510753A JP4781999B2 JP 4781999 B2 JP4781999 B2 JP 4781999B2 JP 2006510753 A JP2006510753 A JP 2006510753A JP 2006510753 A JP2006510753 A JP 2006510753A JP 4781999 B2 JP4781999 B2 JP 4781999B2
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
- A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/0331—Animal model for proliferative diseases
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Endocrinology (AREA)
- Diabetes (AREA)
- Toxicology (AREA)
- Rheumatology (AREA)
- Gastroenterology & Hepatology (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Oncology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
実施例
以下に、本発明を実施例に基づいて更に詳しく説明するが、これらは本発明を何ら限定するものではない。
Claims (14)
- 0〜80℃の温度範囲で水和力が変化するポリマーを表面に被覆した細胞培養支持体上で、ポリマーの水和力の弱い温度域で癌細胞を培養し、その後、培養液をポリマーの水和力の強い状態となる温度に変化させることで培養した癌細胞を蛋白質分解酵素による処理を施すことなく細胞培養支持体から剥離させ、ヒト以外の動物の所定部位に移植することを特徴とするヒト以外の癌細胞移植動物の製造方法。
- 剥離された癌細胞がシート状である、請求項1記載のヒト以外の癌細胞移植動物の製造方法。
- 移植する癌細胞シートを所定の大きさを有する所定の形状とすることで、当該動物の癌組織の大きさ、及びまたは形状を制御する、請求項2記載のヒト以外の癌細胞移植動物の製造方法。
- 剥離方法が培養終了時に培養細胞上にキャリアを密着させ、そのままキャリアと共に剥離する方法である、請求項1〜3のいずれか1項記載のヒト以外の癌細胞移植動物の製造方法。
- 癌細胞が移植可能な細胞株である、請求項1〜4のいずれか1項記載のヒト以外の癌細胞移植動物の製造方法。
- 移植可能な癌細胞がHBC−4、BSY−1、HBC−5、MCF−5、MCF−7、MDA−MB−231、U251、SF−268、SF−295、SF−539、SNB−75、SNB−78、HCC2998、KM−12、HT−29、WiDr、HCT−15、HCT−116、NCI−H23、NCI−H226、NCI−H522、NCI−H460、A549、DMS273、DMS114、LOX−IMVI、OVCAR−3、OVCAR−4、OVCAR−5、OVCAR−8、SK−OV−3、RXF−631L、ACHN、St−4、MKN1、MKN7、MKN28、MKN45、MKN74である、請求項5記載のヒト以外の癌細胞移植動物の製造方法。
- 癌細胞が移植不能な細胞株である、請求項1〜4のいずれか1項記載のヒト以外の癌細胞移植動物の製造方法。
- 移植不能な癌細胞がMGT−40、MGT−90、CS−C9、CS−C20である、請求項7記載のヒト以外の癌細胞移植動物の製造方法。
- 癌細胞が生体組織から採取されたものである、請求項1〜4のいずれか1項記載のヒト以外の癌細胞移植動物の製造方法。
- 移植する細胞数が8×105個以下である、請求項1〜9のいずれか1項記載のヒト以外の癌細胞移植動物の製造方法。
- 癌細胞がヒト由来のものである、請求項1〜10のいずれか1項記載のヒト以外の癌細胞移植動物の製造方法。
- 0〜80℃の温度範囲で水和力が変化するポリマーがポリ(N−イソプロピルアクリルアミド)である、請求項1〜11のいずれか1項記載のヒト以外の癌細胞移植動物の製造方法。
- ヒト以外の癌化モデル動物がヌードマウス、ラット、マウス、モルモット、ウサギであることを特徴とする、請求項1〜12のいずれか1項記載のヒト以外の癌細胞移植動物の製造方法。
- 請求項1〜13のいずれか1項記載の方法によりヒト以外の癌細胞移植動物を製造する際に移植前及び/または移植後に被検物質を投与し、当該被検物質の腫瘍形成能への影響を判定することを特徴とする抗腫瘍剤の選別方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006510753A JP4781999B2 (ja) | 2004-03-04 | 2005-03-04 | 癌細胞移植動物の製造方法 |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004106999 | 2004-03-04 | ||
JP2004106999 | 2004-03-04 | ||
PCT/JP2005/003795 WO2005084429A1 (ja) | 2004-03-04 | 2005-03-04 | 癌細胞移植動物の製造方法 |
JP2006510753A JP4781999B2 (ja) | 2004-03-04 | 2005-03-04 | 癌細胞移植動物の製造方法 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011127662A Division JP5266363B2 (ja) | 2004-03-04 | 2011-06-07 | 癌細胞シート移植動物 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2005084429A1 JPWO2005084429A1 (ja) | 2007-11-29 |
JP4781999B2 true JP4781999B2 (ja) | 2011-09-28 |
Family
ID=34918670
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006510753A Active JP4781999B2 (ja) | 2004-03-04 | 2005-03-04 | 癌細胞移植動物の製造方法 |
JP2011127662A Active JP5266363B2 (ja) | 2004-03-04 | 2011-06-07 | 癌細胞シート移植動物 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
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JP2011127662A Active JP5266363B2 (ja) | 2004-03-04 | 2011-06-07 | 癌細胞シート移植動物 |
Country Status (4)
Country | Link |
---|---|
US (2) | US20080289052A1 (ja) |
EP (1) | EP1731030B1 (ja) |
JP (2) | JP4781999B2 (ja) |
WO (1) | WO2005084429A1 (ja) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060234377A1 (en) * | 2003-02-06 | 2006-10-19 | Teruo Okano | Cell sheets for ectocornea formation, method of producing the same and method of using the same |
EP2267115B8 (en) * | 2003-02-06 | 2014-07-23 | Cellseed Inc. | Anterior ocular segment related cell sheets, three-dimensional structures, and processes for producing the same |
KR101228251B1 (ko) * | 2003-02-20 | 2013-01-30 | 가부시키가이샤 셀시드 | 재생 각막 내피 세포 시트, 제조 방법 및 그 이용 방법 |
WO2005103233A1 (ja) * | 2004-04-25 | 2005-11-03 | Cellseed Inc. | 培養歯根膜細胞シート、製造方法及びその利用方法 |
US10533158B2 (en) * | 2005-02-28 | 2020-01-14 | Tokai University Educational System | Cultured cell sheet, production method thereof, and application method thereof |
EP1859817A4 (en) | 2005-02-28 | 2012-11-07 | Cellseed Inc | CULTIVATED CELL SHEET, PROCESS FOR PRODUCING THE SAME, AND PROCESS FOR RESTORING TISSUE USING THE SAME |
JP5641669B2 (ja) * | 2007-03-14 | 2014-12-17 | 株式会社セルシード | 細胞表層蛋白修復方法 |
CA2719267A1 (en) * | 2008-04-25 | 2009-10-29 | New York Blood Center, Inc. | Abi1/hssh3bp1 conditional knockout mouse |
JP5755882B2 (ja) * | 2008-10-14 | 2015-07-29 | 株式会社セルシード | 温度応答性細胞培養器材、及びその製造方法 |
WO2019132594A1 (ko) * | 2017-12-29 | 2019-07-04 | 한양대학교 산학협력단 | 유전자 전달용 세포 시트 |
JP6846402B2 (ja) * | 2018-12-21 | 2021-03-24 | テルモ株式会社 | シート状細胞培養物とフィブリンゲルとの積層体の製造方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05192138A (ja) * | 1992-01-22 | 1993-08-03 | Kao Corp | 皮膚細胞培養法及び培養皮膚 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06104061B2 (ja) * | 1989-02-10 | 1994-12-21 | 花王株式会社 | 細胞培養支持体材料 |
WO1991001760A1 (en) * | 1989-08-04 | 1991-02-21 | United States Government As Represented By The Secretary Of The Department Of Health And Human Services | Human tumor cells implanted in non-human animals |
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2005
- 2005-03-04 EP EP05720067.7A patent/EP1731030B1/en active Active
- 2005-03-04 WO PCT/JP2005/003795 patent/WO2005084429A1/ja active Application Filing
- 2005-03-04 US US10/591,612 patent/US20080289052A1/en not_active Abandoned
- 2005-03-04 JP JP2006510753A patent/JP4781999B2/ja active Active
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2011
- 2011-06-07 JP JP2011127662A patent/JP5266363B2/ja active Active
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2013
- 2013-07-18 US US13/945,880 patent/US20140056814A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05192138A (ja) * | 1992-01-22 | 1993-08-03 | Kao Corp | 皮膚細胞培養法及び培養皮膚 |
Also Published As
Publication number | Publication date |
---|---|
US20080289052A1 (en) | 2008-11-20 |
JPWO2005084429A1 (ja) | 2007-11-29 |
EP1731030A1 (en) | 2006-12-13 |
EP1731030B1 (en) | 2017-06-21 |
WO2005084429A1 (ja) | 2005-09-15 |
JP5266363B2 (ja) | 2013-08-21 |
US20140056814A1 (en) | 2014-02-27 |
EP1731030A4 (en) | 2009-11-04 |
JP2011212015A (ja) | 2011-10-27 |
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