JP4700805B2 - 多様性の高いライブラリーを製造する方法 - Google Patents
多様性の高いライブラリーを製造する方法 Download PDFInfo
- Publication number
- JP4700805B2 JP4700805B2 JP2000557385A JP2000557385A JP4700805B2 JP 4700805 B2 JP4700805 B2 JP 4700805B2 JP 2000557385 A JP2000557385 A JP 2000557385A JP 2000557385 A JP2000557385 A JP 2000557385A JP 4700805 B2 JP4700805 B2 JP 4700805B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- stranded nucleic
- population
- strand
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Compounds Of Unknown Constitution (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9097098P | 1998-06-29 | 1998-06-29 | |
| US60/090,970 | 1998-06-29 | ||
| PCT/US1999/014776 WO2000000632A1 (en) | 1998-06-29 | 1999-06-29 | Methods for generating highly diverse libraries |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002519038A JP2002519038A (ja) | 2002-07-02 |
| JP2002519038A5 JP2002519038A5 (https=) | 2006-08-31 |
| JP4700805B2 true JP4700805B2 (ja) | 2011-06-15 |
Family
ID=22225171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000557385A Expired - Lifetime JP4700805B2 (ja) | 1998-06-29 | 1999-06-29 | 多様性の高いライブラリーを製造する方法 |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP1092039B1 (https=) |
| JP (1) | JP4700805B2 (https=) |
| KR (1) | KR20010071613A (https=) |
| CN (1) | CN1308683A (https=) |
| AT (1) | ATE547532T1 (https=) |
| AU (1) | AU761570B2 (https=) |
| CA (1) | CA2331926C (https=) |
| ES (1) | ES2381824T3 (https=) |
| IL (1) | IL140100A0 (https=) |
| NO (1) | NO20006675D0 (https=) |
| NZ (1) | NZ508287A (https=) |
| WO (1) | WO2000000632A1 (https=) |
| ZA (1) | ZA200007261B (https=) |
Families Citing this family (103)
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| US6902918B1 (en) | 1998-05-21 | 2005-06-07 | California Institute Of Technology | Oxygenase enzymes and screening method |
| ES2237159T3 (es) | 1998-10-06 | 2005-07-16 | Mark Aaron Emalfarb | Sistema de transformacion en el campo de los hongos micoticos filamentosos en chrysosporium. |
| US7488590B2 (en) | 1998-10-23 | 2009-02-10 | Amgen Inc. | Modified peptides as therapeutic agents |
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| US6368861B1 (en) | 1999-01-19 | 2002-04-09 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
| EP1130093A1 (en) | 1999-01-19 | 2001-09-05 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US7873477B1 (en) | 2001-08-21 | 2011-01-18 | Codexis Mayflower Holdings, Llc | Method and system using systematically varied data libraries |
| US7702464B1 (en) | 2001-08-21 | 2010-04-20 | Maxygen, Inc. | Method and apparatus for codon determining |
| US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US8457903B1 (en) | 1999-01-19 | 2013-06-04 | Codexis Mayflower Holdings, Llc | Method and/or apparatus for determining codons |
| US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| EP1230269A2 (en) | 1999-11-03 | 2002-08-14 | Maxygen, Inc. | Antibody diversity generation |
| US7115403B1 (en) | 2000-05-16 | 2006-10-03 | The California Institute Of Technology | Directed evolution of galactose oxidase enzymes |
| US6539102B1 (en) | 2000-09-01 | 2003-03-25 | Large Scale Proteomics | Reference database |
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| EP1341911A1 (en) * | 2000-12-06 | 2003-09-10 | Novozymes A/S | Method for producing a polynucleotide library |
| US6783941B2 (en) | 2000-12-06 | 2004-08-31 | Novozymes A/S | Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes |
| US6689568B2 (en) | 2001-02-01 | 2004-02-10 | Agilent Technologies, Inc. | Capture arrays using polypeptide capture agents |
| EP1358322B1 (en) * | 2001-02-02 | 2009-10-28 | Large Scale Biology Corporation | A method of increasing complementarity in a heteroduplex polynucleotide |
| AU2002307340A1 (en) | 2001-04-16 | 2002-10-28 | California Institute Of Technology | Peroxide-driven cytochrome p450 oxygenase variants |
| US20030032028A1 (en) * | 2001-06-12 | 2003-02-13 | Gayle Dace | In vitro capture of nucleic acids via modified oligonucleotides and magnetic beads |
| US7226768B2 (en) | 2001-07-20 | 2007-06-05 | The California Institute Of Technology | Cytochrome P450 oxygenases |
| WO2003062417A1 (fr) * | 2002-01-22 | 2003-07-31 | Mitsubishi Chemical Corporation | Produit de ligation arn-adn, et utilisation correspondante |
| EP1493027B1 (en) | 2002-03-01 | 2014-10-15 | Codexis Mayflower Holdings, LLC | Methods, systems, and software for identifying functional bio-molecules |
| US7620500B2 (en) | 2002-03-09 | 2009-11-17 | Maxygen, Inc. | Optimization of crossover points for directed evolution |
| US7226771B2 (en) | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| AU2003243157C1 (en) | 2002-04-19 | 2008-09-04 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| US9321832B2 (en) | 2002-06-28 | 2016-04-26 | Domantis Limited | Ligand |
| WO2005003289A2 (en) | 2002-08-06 | 2005-01-13 | Verdia, Inc. | Ap1 amine oxidase variants |
| DK2194133T3 (en) | 2003-03-06 | 2016-02-29 | Basf Enzymes Llc | Amylases, nucleic acids encoding them, and methods of making and using the same |
| EP2853593B1 (en) | 2003-03-07 | 2017-10-04 | DSM IP Assets B.V. | Hydrolases, nucleic acids encoding them and mehods for making and using them |
| US7592434B2 (en) | 2003-04-04 | 2009-09-22 | Verenium Corporation | Pectate lyases, nucleic encoding them and methods for making and using them |
| CN1863914B (zh) | 2003-04-29 | 2011-03-09 | 先锋高级育种国际公司 | 新的草甘膦-n-乙酰转移酶(gat)基因 |
| WO2005017105A2 (en) | 2003-06-17 | 2005-02-24 | California University Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome p450 |
| CN103484486B (zh) | 2003-07-02 | 2018-04-24 | 维莱尼姆公司 | 葡聚糖酶,编码它们的核酸以及制备和使用它们的方法 |
| WO2005017116A2 (en) | 2003-08-11 | 2005-02-24 | California Institute Of Technology | Thermostable peroxide-driven cytochrome p450 oxygenase variants and methods of use |
| EP1668113B1 (en) | 2003-08-11 | 2013-06-19 | Verenium Corporation | Laccases, nucleic acids encoding them and methods for making and using them |
| JP2005065575A (ja) * | 2003-08-22 | 2005-03-17 | Japan Science & Technology Agency | フレームシャッフリングによるタンパク質分子多様性集団の作製 |
| CN101432292B (zh) | 2004-06-16 | 2013-03-13 | 维莱尼姆公司 | 对叶绿素进行酶促脱色的组合物和方法 |
| EP2886658A1 (en) | 2005-03-10 | 2015-06-24 | BASF Enzymes LLC | Lyase enzymes, nucleic acids encoding them and methods for making and using them |
| MX300732B (es) | 2005-03-15 | 2012-06-28 | Verenium Corp | Celulasas, acidos nucleicos que las codifican y metodos para hacerlas y usarlas. |
| US11214817B2 (en) | 2005-03-28 | 2022-01-04 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
| US8715988B2 (en) | 2005-03-28 | 2014-05-06 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
| US20090324574A1 (en) | 2006-02-02 | 2009-12-31 | Verenium Corporation | Esterases and Related Nucleic Acids and Methods |
| WO2007094852A2 (en) | 2006-02-10 | 2007-08-23 | Verenium Corporation | Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them |
| EP2548955A1 (en) | 2006-02-14 | 2013-01-23 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
| MX2008011477A (es) | 2006-03-07 | 2008-09-23 | Cargill Inc | Aldolasas, acidos nucleicos que las codifican y metodos para hacerlas y usarlas. |
| BRPI0708614A2 (pt) | 2006-03-07 | 2011-06-07 | Verenium Corp | aldolases, ácidos nucléicos codificando as mesmas e métodos para produzir e usar as mesmas |
| EP2069513A4 (en) | 2006-08-04 | 2012-03-21 | California Inst Of Techn | METHOD AND SYSTEMS FOR THE SELECTIVE FLUORINATION OF ORGANIC MOLECULES |
| US8252559B2 (en) | 2006-08-04 | 2012-08-28 | The California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
| BRPI0714876B1 (pt) | 2006-08-04 | 2022-04-19 | Verenium Corporation | Ácido nucleico isolado, sintético ou recombinante, cassete de expressão, vetor ou veículo de clonagem, célula bacteriana, fúngica ou de levedura transformada, polipeptídeo isolado, sintético ou recombinante, composição, bem como métodos de produção e de usos dos mesmos |
| JP5307013B2 (ja) | 2006-09-21 | 2013-10-02 | ヴェレニウム コーポレイション | フィターゼ、フィターゼをコードする核酸、並びにフィターゼを製造および使用する方法 |
| AR062947A1 (es) | 2006-09-21 | 2008-12-17 | Verenium Corp | Fosfolipasas acidos nucleicos que las codifican y metodos para prepararlas y usarlas |
| EP2505651A3 (en) | 2006-12-10 | 2013-01-09 | Dyadic International, Inc. | Isolated fungus with reduced protease activity |
| PL2069490T5 (pl) | 2006-12-21 | 2018-09-28 | Basf Enzymes Llc | Amylazy i glukoamylazy, kwasy nukleinowe kodujące te związki oraz sposoby wytwarzania tych związków oraz stosowania ich |
| NZ578309A (en) | 2007-01-30 | 2012-06-29 | Verenium Corp | Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them |
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| WO2009045627A2 (en) | 2007-10-03 | 2009-04-09 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
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| DK2238261T3 (da) | 2008-01-03 | 2014-02-10 | Verenium Corp | Isomeraser, nukleinsyrer der koder for dem og fremgangsmåder til fremstilling og anvendelse deraf |
| WO2009143397A2 (en) | 2008-05-23 | 2009-11-26 | E. I. Du Pont De Nemours And Company | Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
| US8198062B2 (en) | 2008-08-29 | 2012-06-12 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and methods for making and using them |
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| CN103237894A (zh) | 2010-08-13 | 2013-08-07 | 先锋国际良种公司 | 包含具有羟基苯丙酮酸双加氧酶(hppd)活性的序列的组合物和方法 |
| WO2013016207A2 (en) | 2011-07-22 | 2013-01-31 | California Institute Of Technology | Stable fungal cel6 enzyme variants |
| BR112014027468A2 (pt) | 2012-05-04 | 2017-06-27 | Du Pont | polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo. |
| JP6004423B2 (ja) * | 2012-05-07 | 2016-10-05 | 国立研究開発法人産業技術総合研究所 | 遺伝子連結法およびそれを用いた単鎖抗体作製方法 |
| AU2014236154A1 (en) | 2013-03-14 | 2015-09-17 | Pioneer Hi-Bred International, Inc. | Compositions having dicamba decarboxylase activity and methods of use |
| BR112015023286A2 (pt) | 2013-03-14 | 2018-03-06 | Arzeda Corp | polipeptídeo recombinante com atividade da dicamba descarboxilase, construto de polinucleotídeo, célula, método de produção de uma célula hospedeira compreendendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade da dicamba descarboxilase, método para descarboxilar dicamba, um derivado de dicamba ou um metabolito de dicamba, método para a detecção de um polipeptideo e método para a detecção da presença de um polinucleotideo que codifica um polipeptideo tendo atividade da dicamba descarboxilase |
| CA2901316A1 (en) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Phi-4 polypeptides and methods for their use |
| MX359026B (es) | 2013-08-16 | 2018-09-12 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos de uso. |
| CA3223359A1 (en) | 2013-09-13 | 2015-03-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| EP3087088B1 (en) | 2013-12-23 | 2022-05-25 | University Of Rochester | Methods and compositions for ribosomal synthesis of macrocyclic peptides |
| WO2015120270A1 (en) | 2014-02-07 | 2015-08-13 | Pioneer Hi Bred International, Inc. | Insecticidal proteins and methods for their use |
| CA3290904A1 (en) | 2014-02-07 | 2026-03-02 | E. I. Du Pont De Nemours And Company | Insecticidal proteins and methods for their use |
| CA2963558C (en) | 2014-10-16 | 2023-04-04 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CN107635396B (zh) | 2015-01-15 | 2021-12-24 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| WO2016138292A1 (en) * | 2015-02-25 | 2016-09-01 | Igenomx International Genomics Corporation | Methods and compositions for in silico long read sequencing |
| CN107529763B (zh) | 2015-03-11 | 2021-08-20 | 先锋国际良种公司 | Pip-72的杀昆虫组合及使用方法 |
| CN116333064A (zh) | 2015-05-19 | 2023-06-27 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| CA3268091A1 (en) | 2015-08-06 | 2026-03-02 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
| WO2017105987A1 (en) | 2015-12-18 | 2017-06-22 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
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| MX387077B (es) | 2016-05-04 | 2025-03-19 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para sus usos. |
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| MX387927B (es) | 2016-11-01 | 2025-03-19 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
| EP3555118B1 (en) | 2016-12-14 | 2021-08-18 | Pioneer Hi-Bred International Inc. | Insecticidal proteins and methods for their use |
| WO2018118811A1 (en) | 2016-12-22 | 2018-06-28 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| MX2019009371A (es) | 2017-02-08 | 2019-09-23 | Pionner Hi Bred Int Inc | Combinaciones insecticidas de proteinas insecticidas derivadas de plantas y metodos para su uso. |
| MX2019013321A (es) | 2017-05-11 | 2020-02-10 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
| CN110158157B (zh) * | 2018-02-13 | 2021-02-02 | 浙江大学 | 基于模板材料合成长度固定和特定末端序列dna文库的方法 |
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| CN111867377B (zh) | 2018-03-14 | 2023-05-23 | 先锋国际良种公司 | 来自植物的杀昆虫蛋白及其使用方法 |
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| US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
-
1999
- 1999-06-29 AT AT99932081T patent/ATE547532T1/de active
- 1999-06-29 IL IL14010099A patent/IL140100A0/xx unknown
- 1999-06-29 JP JP2000557385A patent/JP4700805B2/ja not_active Expired - Lifetime
- 1999-06-29 CA CA2331926A patent/CA2331926C/en not_active Expired - Lifetime
- 1999-06-29 NZ NZ508287A patent/NZ508287A/en not_active IP Right Cessation
- 1999-06-29 CN CN99807977A patent/CN1308683A/zh active Pending
- 1999-06-29 KR KR1020007014838A patent/KR20010071613A/ko not_active Withdrawn
- 1999-06-29 WO PCT/US1999/014776 patent/WO2000000632A1/en not_active Ceased
- 1999-06-29 EP EP99932081A patent/EP1092039B1/en not_active Expired - Lifetime
- 1999-06-29 ES ES99932081T patent/ES2381824T3/es not_active Expired - Lifetime
- 1999-06-29 AU AU48470/99A patent/AU761570B2/en not_active Expired
-
2000
- 2000-12-07 ZA ZA200007261A patent/ZA200007261B/en unknown
- 2000-12-28 NO NO20006675A patent/NO20006675D0/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| ES2381824T3 (es) | 2012-05-31 |
| NZ508287A (en) | 2003-08-29 |
| ZA200007261B (en) | 2002-05-15 |
| WO2000000632A1 (en) | 2000-01-06 |
| CN1308683A (zh) | 2001-08-15 |
| CA2331926C (en) | 2013-09-10 |
| KR20010071613A (ko) | 2001-07-28 |
| EP1092039A4 (en) | 2003-07-02 |
| EP1092039A1 (en) | 2001-04-18 |
| EP1092039B1 (en) | 2012-02-29 |
| IL140100A0 (en) | 2002-02-10 |
| ATE547532T1 (de) | 2012-03-15 |
| JP2002519038A (ja) | 2002-07-02 |
| NO20006675L (no) | 2000-12-28 |
| AU4847099A (en) | 2000-01-17 |
| CA2331926A1 (en) | 2000-01-06 |
| WO2000000632A9 (en) | 2000-10-26 |
| NO20006675D0 (no) | 2000-12-28 |
| AU761570B2 (en) | 2003-06-05 |
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