KR20010071613A - 매우 다양한 라이브러리의 생산방법 - Google Patents
매우 다양한 라이브러리의 생산방법 Download PDFInfo
- Publication number
- KR20010071613A KR20010071613A KR1020007014838A KR20007014838A KR20010071613A KR 20010071613 A KR20010071613 A KR 20010071613A KR 1020007014838 A KR1020007014838 A KR 1020007014838A KR 20007014838 A KR20007014838 A KR 20007014838A KR 20010071613 A KR20010071613 A KR 20010071613A
- Authority
- KR
- South Korea
- Prior art keywords
- nucleic acid
- stranded nucleic
- population
- template
- strand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
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- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 142
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 114
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 114
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- 108010061982 DNA Ligases Proteins 0.000 claims abstract description 13
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- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
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- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- MWWATHDPGQKSAR-UHFFFAOYSA-N propyne Chemical compound CC#C MWWATHDPGQKSAR-UHFFFAOYSA-N 0.000 description 2
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 2
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- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
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- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
- 108010063113 DNA Polymerase II Proteins 0.000 description 1
- 102000010567 DNA Polymerase II Human genes 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical group S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108091027305 Heteroduplex Proteins 0.000 description 1
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- 108060004795 Methyltransferase Proteins 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- GEYBMYRBIABFTA-VIFPVBQESA-N O-methyl-L-tyrosine Chemical compound COC1=CC=C(C[C@H](N)C(O)=O)C=C1 GEYBMYRBIABFTA-VIFPVBQESA-N 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
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- 108020005038 Terminator Codon Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
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- 150000001408 amides Chemical class 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
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- 230000003197 catalytic effect Effects 0.000 description 1
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- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
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- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Compounds Of Unknown Constitution (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9097098P | 1998-06-29 | 1998-06-29 | |
| US60/090,970 | 1998-06-29 | ||
| PCT/US1999/014776 WO2000000632A1 (en) | 1998-06-29 | 1999-06-29 | Methods for generating highly diverse libraries |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20010071613A true KR20010071613A (ko) | 2001-07-28 |
Family
ID=22225171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020007014838A Withdrawn KR20010071613A (ko) | 1998-06-29 | 1999-06-29 | 매우 다양한 라이브러리의 생산방법 |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP1092039B1 (https=) |
| JP (1) | JP4700805B2 (https=) |
| KR (1) | KR20010071613A (https=) |
| CN (1) | CN1308683A (https=) |
| AT (1) | ATE547532T1 (https=) |
| AU (1) | AU761570B2 (https=) |
| CA (1) | CA2331926C (https=) |
| ES (1) | ES2381824T3 (https=) |
| IL (1) | IL140100A0 (https=) |
| NO (1) | NO20006675D0 (https=) |
| NZ (1) | NZ508287A (https=) |
| WO (1) | WO2000000632A1 (https=) |
| ZA (1) | ZA200007261B (https=) |
Families Citing this family (103)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| US6902918B1 (en) | 1998-05-21 | 2005-06-07 | California Institute Of Technology | Oxygenase enzymes and screening method |
| KR100618495B1 (ko) | 1998-10-06 | 2006-08-31 | 마크 아론 에말파브 | 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서 |
| US7488590B2 (en) | 1998-10-23 | 2009-02-10 | Amgen Inc. | Modified peptides as therapeutic agents |
| US6368861B1 (en) | 1999-01-19 | 2002-04-09 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US6917882B2 (en) | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US7873477B1 (en) | 2001-08-21 | 2011-01-18 | Codexis Mayflower Holdings, Llc | Method and system using systematically varied data libraries |
| US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
| US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| ES2341217T3 (es) | 1999-01-19 | 2010-06-17 | Maxygen, Inc. | Recombinacion de acidos nucleicos mediada por oligonucleotidos. |
| US8457903B1 (en) | 1999-01-19 | 2013-06-04 | Codexis Mayflower Holdings, Llc | Method and/or apparatus for determining codons |
| US7702464B1 (en) | 2001-08-21 | 2010-04-20 | Maxygen, Inc. | Method and apparatus for codon determining |
| US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| AU1456101A (en) | 1999-11-03 | 2001-05-14 | Maxygen, Inc. | Antibody diversity generation |
| US7115403B1 (en) | 2000-05-16 | 2006-10-03 | The California Institute Of Technology | Directed evolution of galactose oxidase enzymes |
| US6980674B2 (en) | 2000-09-01 | 2005-12-27 | Large Scale Proteomics Corp. | Reference database |
| US6539102B1 (en) | 2000-09-01 | 2003-03-25 | Large Scale Proteomics | Reference database |
| WO2005012515A2 (en) | 2003-04-29 | 2005-02-10 | Pioneer Hi-Bred International, Inc. | Novel glyphosate-n-acetyltransferase (gat) genes |
| US6783941B2 (en) | 2000-12-06 | 2004-08-31 | Novozymes A/S | Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes |
| AU2002220529A1 (en) * | 2000-12-06 | 2002-06-18 | Novozymes A/S | Method for producing a polynucleotide library |
| US6689568B2 (en) | 2001-02-01 | 2004-02-10 | Agilent Technologies, Inc. | Capture arrays using polypeptide capture agents |
| CA2436214C (en) * | 2001-02-02 | 2012-12-04 | Large Scale Biology Corporation | A method of increasing complementarity in a heteroduplex polynucleotide |
| AU2002307340A1 (en) | 2001-04-16 | 2002-10-28 | California Institute Of Technology | Peroxide-driven cytochrome p450 oxygenase variants |
| US20030032028A1 (en) * | 2001-06-12 | 2003-02-13 | Gayle Dace | In vitro capture of nucleic acids via modified oligonucleotides and magnetic beads |
| US7226768B2 (en) | 2001-07-20 | 2007-06-05 | The California Institute Of Technology | Cytochrome P450 oxygenases |
| WO2003062417A1 (fr) * | 2002-01-22 | 2003-07-31 | Mitsubishi Chemical Corporation | Produit de ligation arn-adn, et utilisation correspondante |
| JP5319865B2 (ja) | 2002-03-01 | 2013-10-16 | コデクシス メイフラワー ホールディングス， エルエルシー | 機能的生体分子を同定する方法、システム、およびソフトウェア |
| EP1488335A4 (en) | 2002-03-09 | 2006-11-15 | Maxygen Inc | OPTIMIZING CROSSOVER POINTS FOR THE ADJUSTED EVOLUTION |
| US7226771B2 (en) | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| PT1497418E (pt) | 2002-04-19 | 2013-01-25 | Dsm Ip Assets Bv | Fosfolípases, ácidos nucleicos que as codificam e métodos para as preparar e utilizar |
| US9321832B2 (en) | 2002-06-28 | 2016-04-26 | Domantis Limited | Ligand |
| BR0313281A (pt) | 2002-08-06 | 2007-07-24 | Verdia Inc | variantes de ap1 amina oxidase |
| CN102618564B (zh) | 2003-03-06 | 2014-10-29 | 维莱尼姆公司 | 淀粉酶、编码它们的核酸及其制备和应用方法 |
| CA3007908A1 (en) | 2003-03-07 | 2005-04-14 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and methods for making and using them |
| HUE030493T2 (en) | 2003-04-04 | 2017-05-29 | Basf Enzymes Llc | Pectate lyases, nucleic acids and processes encoding them for their production and use |
| EP1639091B1 (en) | 2003-06-17 | 2012-12-05 | California University Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome p450 |
| US7960148B2 (en) | 2003-07-02 | 2011-06-14 | Verenium Corporation | Glucanases, nucleic acids encoding them and methods for making and using them |
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1999
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- 1999-06-29 AT AT99932081T patent/ATE547532T1/de active
- 1999-06-29 EP EP99932081A patent/EP1092039B1/en not_active Expired - Lifetime
- 1999-06-29 JP JP2000557385A patent/JP4700805B2/ja not_active Expired - Lifetime
- 1999-06-29 CN CN99807977A patent/CN1308683A/zh active Pending
- 1999-06-29 NZ NZ508287A patent/NZ508287A/en not_active IP Right Cessation
- 1999-06-29 ES ES99932081T patent/ES2381824T3/es not_active Expired - Lifetime
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- 1999-06-29 WO PCT/US1999/014776 patent/WO2000000632A1/en not_active Ceased
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| EP1092039A1 (en) | 2001-04-18 |
| WO2000000632A9 (en) | 2000-10-26 |
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| AU4847099A (en) | 2000-01-17 |
| AU761570B2 (en) | 2003-06-05 |
| WO2000000632A1 (en) | 2000-01-06 |
| CN1308683A (zh) | 2001-08-15 |
| CA2331926C (en) | 2013-09-10 |
| JP4700805B2 (ja) | 2011-06-15 |
| IL140100A0 (en) | 2002-02-10 |
| ATE547532T1 (de) | 2012-03-15 |
| CA2331926A1 (en) | 2000-01-06 |
| NZ508287A (en) | 2003-08-29 |
| NO20006675D0 (no) | 2000-12-28 |
| ZA200007261B (en) | 2002-05-15 |
| JP2002519038A (ja) | 2002-07-02 |
| EP1092039B1 (en) | 2012-02-29 |
| EP1092039A4 (en) | 2003-07-02 |
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