JP4651282B2 - 造血幹細胞及び造血前駆細胞の増幅方法 - Google Patents

造血幹細胞及び造血前駆細胞の増幅方法 Download PDF

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Publication number
JP4651282B2
JP4651282B2 JP2004013291A JP2004013291A JP4651282B2 JP 4651282 B2 JP4651282 B2 JP 4651282B2 JP 2004013291 A JP2004013291 A JP 2004013291A JP 2004013291 A JP2004013291 A JP 2004013291A JP 4651282 B2 JP4651282 B2 JP 4651282B2
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Japan
Prior art keywords
cells
serum
hematopoietic
hematopoietic stem
medium
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Expired - Fee Related
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JP2004013291A
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English (en)
Japanese (ja)
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JP2005204539A (ja
JP2005204539A5 (enExample
Inventor
麻子 初山
仁也 伊藤
龍俊 中畑
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Foundation for Biomedical Research and Innovation at Kobe
Tanabe Pharma Corp
Original Assignee
Mitsubishi Tanabe Pharma Corp
Foundation for Biomedical Research and Innovation at Kobe
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Application filed by Mitsubishi Tanabe Pharma Corp, Foundation for Biomedical Research and Innovation at Kobe filed Critical Mitsubishi Tanabe Pharma Corp
Priority to JP2004013291A priority Critical patent/JP4651282B2/ja
Priority to CA2554192A priority patent/CA2554192C/en
Priority to PCT/JP2004/010677 priority patent/WO2005071064A1/ja
Priority to US10/587,014 priority patent/US8168429B2/en
Publication of JP2005204539A publication Critical patent/JP2005204539A/ja
Publication of JP2005204539A5 publication Critical patent/JP2005204539A5/ja
Application granted granted Critical
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2004013291A 2004-01-21 2004-01-21 造血幹細胞及び造血前駆細胞の増幅方法 Expired - Fee Related JP4651282B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2004013291A JP4651282B2 (ja) 2004-01-21 2004-01-21 造血幹細胞及び造血前駆細胞の増幅方法
CA2554192A CA2554192C (en) 2004-01-21 2004-07-21 Method of amplifying hematopoietic stem cell and hematopoietic progenitor cell
PCT/JP2004/010677 WO2005071064A1 (ja) 2004-01-21 2004-07-21 造血幹細胞及び造血前駆細胞の増幅方法
US10/587,014 US8168429B2 (en) 2004-01-21 2004-07-21 Method of amplifying hematopoietic stem cell and hematopoietic progenitor cell

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2004013291A JP4651282B2 (ja) 2004-01-21 2004-01-21 造血幹細胞及び造血前駆細胞の増幅方法

Publications (3)

Publication Number Publication Date
JP2005204539A JP2005204539A (ja) 2005-08-04
JP2005204539A5 JP2005204539A5 (enExample) 2007-03-08
JP4651282B2 true JP4651282B2 (ja) 2011-03-16

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Family Applications (1)

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JP2004013291A Expired - Fee Related JP4651282B2 (ja) 2004-01-21 2004-01-21 造血幹細胞及び造血前駆細胞の増幅方法

Country Status (4)

Country Link
US (1) US8168429B2 (enExample)
JP (1) JP4651282B2 (enExample)
CA (1) CA2554192C (enExample)
WO (1) WO2005071064A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200530269A (en) 2003-12-12 2005-09-16 Chugai Pharmaceutical Co Ltd Anti-Mpl antibodies
JP4921083B2 (ja) * 2005-09-13 2012-04-18 タカラバイオ株式会社 レトロウィルス産生用無血清培地
EP2044956A4 (en) * 2006-06-14 2010-08-11 Chugai Pharmaceutical Co Ltd PROMOTER OF THE PROLIFERATION OF HEMATOPOIETIC STEM CELLS
WO2008009642A1 (en) * 2006-07-17 2008-01-24 Novozymes A/S Cell culture media
DK2194121T3 (en) 2007-09-11 2016-12-12 Univ Sapporo Medical CELL GROWTH PROCESS AND PHARMACEUTICAL COMPOSITION FOR tissue repair and regeneration
CN101889007A (zh) * 2007-12-06 2010-11-17 日产化学工业株式会社 采用杂环化合物的造血干细胞的扩增方法
US20110158955A1 (en) 2008-03-11 2011-06-30 Yoshimoto Katsura Method for producing cells having characteristic of hematopoietic stem cells/progenitor cells
WO2010140685A1 (ja) * 2009-06-04 2010-12-09 日産化学工業株式会社 ヘテロ環化合物及び造血幹細胞の増幅剤
US9315776B2 (en) 2011-11-09 2016-04-19 National University Of Singapore Wharton's jelly mesenchymal stem cells and uses thereof
SG10201701112QA (en) 2012-08-15 2017-04-27 Univ Singapore Wound dressing nanomesh impregnated with human umbilical cord wharton's jelly stem cells
US9402388B2 (en) * 2012-11-01 2016-08-02 National University Of Singapore Methods of freezing stem cells
JP2017532325A (ja) * 2014-10-03 2017-11-02 イエール ユニバーシティ 抗癌療法のための自然免疫系の改変
TWI548748B (zh) * 2015-04-16 2016-09-11 台灣尖端先進生技醫藥股份有限公司 造血幹／先驅細胞之體外放大培養方法及其立即可使用之組成物
TWI645039B (zh) * 2016-12-11 2018-12-21 生寶生物科技股份有限公司 無血清培養基及擴增造血幹細胞的方法
CN114686432B (zh) * 2020-12-28 2024-02-02 苏州方舟生物科技有限公司 一种中性粒细胞的高效扩增培养体系及其应用
WO2023275880A1 (en) * 2021-06-30 2023-01-05 Regrow Biosciences Private Limited Method of obtaining hsc population, t cell population and nk cell population and compositions thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL66614A (en) * 1981-08-28 1985-09-29 Genentech Inc Method of constructing a dna sequence encoding a polypeptide,microbial production of human serum albumin,and pharmaceutical compositions comprising it
US5635386A (en) * 1989-06-15 1997-06-03 The Regents Of The University Of Michigan Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture
US5437994A (en) * 1989-06-15 1995-08-01 Regents Of The University Of Michigan Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
US5405772A (en) * 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells
US6037174A (en) * 1993-08-23 2000-03-14 Nexell Therapeutics, Inc. Preparation of serum-free suspensions of human hematopoietic cells or precursor cells
AU702871B2 (en) * 1993-08-23 1999-03-11 Nexell Therapeutics Inc. (In vitro) growth of neutrophil and megakaryocyte precursors in serum-free media
US5505908A (en) * 1994-09-01 1996-04-09 Halozone Technologies, Inc. Recycling and recovery of methyl bromide fumigant
EP0891419A4 (en) 1996-03-12 2000-03-01 Life Technologies Inc NUTRIENT ADDITIVE FOR HEMATOPOETIC CELL CULTURES
DE69733694T2 (de) * 1996-07-10 2006-07-06 Muramatsu, Takashi, Nagoya Verwendung von proteinen aus der mk familie als hämatopoietischer faktor
WO1998006822A1 (fr) * 1996-08-08 1998-02-19 Yoshitomi Pharmaceutical Industries, Ltd. Milieu de culture et ses applications
US5945337A (en) * 1996-10-18 1999-08-31 Quality Biological, Inc. Method for culturing CD34+ cells in a serum-free medium
WO2004029200A2 (en) * 2002-09-26 2004-04-08 Children's Medical Center Corporation Method of enhancing proliferation and/or hematopoietic differentiation of stem cells
WO2005070120A2 (en) * 2004-01-09 2005-08-04 Serologicals Investment Company, Inc. Cell culture media

Also Published As

Publication number Publication date
JP2005204539A (ja) 2005-08-04
WO2005071064A1 (ja) 2005-08-04
CA2554192A1 (en) 2005-08-04
CA2554192C (en) 2013-09-10
US8168429B2 (en) 2012-05-01
US20070166825A1 (en) 2007-07-19

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