CA2554192C - Method of amplifying hematopoietic stem cell and hematopoietic progenitor cell - Google Patents

Method of amplifying hematopoietic stem cell and hematopoietic progenitor cell Download PDF

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Publication number
CA2554192C
CA2554192C CA2554192A CA2554192A CA2554192C CA 2554192 C CA2554192 C CA 2554192C CA 2554192 A CA2554192 A CA 2554192A CA 2554192 A CA2554192 A CA 2554192A CA 2554192 C CA2554192 C CA 2554192C
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CA
Canada
Prior art keywords
cell
serum
hematopoietic
cells
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2554192A
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English (en)
French (fr)
Other versions
CA2554192A1 (en
Inventor
Asako Hatsuyama
Kiminari Ito
Tatsutoshi Nakahata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
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Mitsubishi Tanabe Pharma Corp
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Publication of CA2554192A1 publication Critical patent/CA2554192A1/en
Application granted granted Critical
Publication of CA2554192C publication Critical patent/CA2554192C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2554192A 2004-01-21 2004-07-21 Method of amplifying hematopoietic stem cell and hematopoietic progenitor cell Expired - Fee Related CA2554192C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2004013291A JP4651282B2 (ja) 2004-01-21 2004-01-21 造血幹細胞及び造血前駆細胞の増幅方法
JP2004-013291 2004-01-21
PCT/JP2004/010677 WO2005071064A1 (ja) 2004-01-21 2004-07-21 造血幹細胞及び造血前駆細胞の増幅方法

Publications (2)

Publication Number Publication Date
CA2554192A1 CA2554192A1 (en) 2005-08-04
CA2554192C true CA2554192C (en) 2013-09-10

Family

ID=34805380

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2554192A Expired - Fee Related CA2554192C (en) 2004-01-21 2004-07-21 Method of amplifying hematopoietic stem cell and hematopoietic progenitor cell

Country Status (4)

Country Link
US (1) US8168429B2 (enExample)
JP (1) JP4651282B2 (enExample)
CA (1) CA2554192C (enExample)
WO (1) WO2005071064A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200530269A (en) 2003-12-12 2005-09-16 Chugai Pharmaceutical Co Ltd Anti-Mpl antibodies
JP4921083B2 (ja) * 2005-09-13 2012-04-18 タカラバイオ株式会社 レトロウィルス産生用無血清培地
RU2009100930A (ru) * 2006-06-14 2010-07-20 Чугаи Сейяку Кабусики Кайся (Jp) Лекарственные средства для стимуляции роста гемопоэтических стволовых клеток
WO2008009642A1 (en) * 2006-07-17 2008-01-24 Novozymes A/S Cell culture media
PL2194121T3 (pl) 2007-09-11 2017-02-28 Sapporo Medical University Sposób hodowania komórek i preparat farmaceutyczny do naprawy i regeneracji tkanek
US9527828B2 (en) 2007-12-06 2016-12-27 Nissan Chemical Industries, Ltd. Method for expanding hematopoietic stem cells using heterocyclic compound
JP5660451B2 (ja) 2008-03-11 2015-01-28 独立行政法人理化学研究所 造血幹／前駆細胞の特性を有する細胞の製造方法
CN102448949B (zh) * 2009-06-04 2014-09-03 日产化学工业株式会社 杂环化合物及造血干细胞的扩增剂
US9315776B2 (en) 2011-11-09 2016-04-19 National University Of Singapore Wharton's jelly mesenchymal stem cells and uses thereof
SG11201501024QA (en) 2012-08-15 2015-03-30 Univ Singapore Wound dressing nanomesh impregnated with human umbilical cord wharton's jelly stem cells
US9402388B2 (en) * 2012-11-01 2016-08-02 National University Of Singapore Methods of freezing stem cells
JP2017532325A (ja) * 2014-10-03 2017-11-02 イエール ユニバーシティ 抗癌療法のための自然免疫系の改変
TWI548748B (zh) * 2015-04-16 2016-09-11 台灣尖端先進生技醫藥股份有限公司 造血幹／先驅細胞之體外放大培養方法及其立即可使用之組成物
TWI645039B (zh) * 2016-12-11 2018-12-21 生寶生物科技股份有限公司 無血清培養基及擴增造血幹細胞的方法
CN114686432B (zh) * 2020-12-28 2024-02-02 苏州方舟生物科技有限公司 一种中性粒细胞的高效扩增培养体系及其应用
US20240287458A1 (en) * 2021-06-30 2024-08-29 Regrow Biosciences Private Limited Method of obtaining hsc population, t cell population and nk cell population and compositions thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL66614A (en) * 1981-08-28 1985-09-29 Genentech Inc Method of constructing a dna sequence encoding a polypeptide,microbial production of human serum albumin,and pharmaceutical compositions comprising it
US5437994A (en) * 1989-06-15 1995-08-01 Regents Of The University Of Michigan Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
US5635386A (en) * 1989-06-15 1997-06-03 The Regents Of The University Of Michigan Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture
US5405772A (en) * 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells
US6037174A (en) * 1993-08-23 2000-03-14 Nexell Therapeutics, Inc. Preparation of serum-free suspensions of human hematopoietic cells or precursor cells
WO1995006112A1 (en) 1993-08-23 1995-03-02 Baxter International Inc. In vitro growth of neutrophil and megakaryocyte precursors in serum-free media
US5505908A (en) 1994-09-01 1996-04-09 Halozone Technologies, Inc. Recycling and recovery of methyl bromide fumigant
CA2248142A1 (en) * 1996-03-12 1997-09-18 Life Technologies, Inc. Hematopoietic cell culture nutrient supplement
ATE299030T1 (de) * 1996-07-10 2005-07-15 Meiji Dairies Corp Verwendung von proteinen aus der mk familie als hämatopoietischer faktor
WO1998006822A1 (fr) * 1996-08-08 1998-02-19 Yoshitomi Pharmaceutical Industries, Ltd. Milieu de culture et ses applications
US5945337A (en) * 1996-10-18 1999-08-31 Quality Biological, Inc. Method for culturing CD34+ cells in a serum-free medium
US7427603B2 (en) * 2002-09-26 2008-09-23 The Children's Medical Center Corporation Method of enhancing proliferation and/or hematopoietic differentiation of stem cells
US20060073591A1 (en) * 2004-01-09 2006-04-06 Abitorabi M A Cell culture media

Also Published As

Publication number Publication date
US20070166825A1 (en) 2007-07-19
JP4651282B2 (ja) 2011-03-16
CA2554192A1 (en) 2005-08-04
JP2005204539A (ja) 2005-08-04
US8168429B2 (en) 2012-05-01
WO2005071064A1 (ja) 2005-08-04

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