JP4510626B2 - 脱塩基部位エンドヌクレアーゼアッセイ - Google Patents

脱塩基部位エンドヌクレアーゼアッセイ Download PDF

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JP4510626B2
JP4510626B2 JP2004529748A JP2004529748A JP4510626B2 JP 4510626 B2 JP4510626 B2 JP 4510626B2 JP 2004529748 A JP2004529748 A JP 2004529748A JP 2004529748 A JP2004529748 A JP 2004529748A JP 4510626 B2 JP4510626 B2 JP 4510626B2
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probe
nucleic acid
site
target nucleic
endonuclease
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JP2005536210A (ja
JP2005536210A5 (enExample
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イゴール・ヴェー・クトヤヴィン
デイビッド・マイルシ
マール・ホークストラ
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エポック・バイオサイエンシーズ・インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
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  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2004529748A 2002-08-21 2003-08-20 脱塩基部位エンドヌクレアーゼアッセイ Expired - Fee Related JP4510626B2 (ja)

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Application Number Priority Date Filing Date Title
US40564202P 2002-08-21 2002-08-21
PCT/US2003/026133 WO2004018626A2 (en) 2002-08-21 2003-08-20 Abasic site endonuclease assay

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JP2005536210A JP2005536210A (ja) 2005-12-02
JP2005536210A5 JP2005536210A5 (enExample) 2006-09-28
JP4510626B2 true JP4510626B2 (ja) 2010-07-28

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JP2004529748A Expired - Fee Related JP4510626B2 (ja) 2002-08-21 2003-08-20 脱塩基部位エンドヌクレアーゼアッセイ

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US (3) US7252940B2 (enExample)
EP (1) EP1543154A4 (enExample)
JP (1) JP4510626B2 (enExample)
AU (1) AU2003274914A1 (enExample)
CA (1) CA2494993A1 (enExample)
WO (1) WO2004018626A2 (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE400663T1 (de) 2002-02-21 2008-07-15 Asm Scient Inc Rekombinase-polymerase-amplifikation
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
WO2004018626A2 (en) * 2002-08-21 2004-03-04 Epoch Biosciences, Inc. Abasic site endonuclease assay
EP2829615B1 (en) 2005-07-25 2018-05-09 Alere San Diego, Inc. Kit for multiplexing recombinase polymerase amplification
US8349556B2 (en) 2006-04-28 2013-01-08 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates to improve nucleic acid detection
US9121056B2 (en) * 2006-04-28 2015-09-01 Igor Kutyavin Use of products of PCR amplification carrying elements of secondary structure to improve PCR-based nucleic acid detection
JP2009535053A (ja) * 2006-05-04 2009-10-01 エーエスエム サイエンティフィック， インコーポレイテッド レコンビナーゼポリメラーゼ増幅
WO2008002920A2 (en) * 2006-06-26 2008-01-03 Epoch Biosciences, Inc. Methods for generating target nucleic acid sequences
JP5088034B2 (ja) * 2006-08-14 2012-12-05 ソニー株式会社 物質の検出等に有用な核酸鎖とその方法
US7727745B2 (en) * 2006-08-14 2010-06-01 Bio-Id Diagnostic Inc. Synthesis of single-stranded DNA
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
US8143006B2 (en) * 2007-08-03 2012-03-27 Igor Kutyavin Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking
ATE549419T1 (de) 2007-08-29 2012-03-15 Sequenom Inc Verfahren und zusammensetzungen für die universelle grössenspezifische polymerasekettenreaktion
US20110171649A1 (en) * 2008-09-10 2011-07-14 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
US8129149B1 (en) 2008-11-12 2012-03-06 The United States Of America As Represented By The Secretary Of The Army Rapid and sensitive method to measure endonuclease activity
WO2010067055A1 (en) * 2008-12-09 2010-06-17 Oxitec Ltd. Enhanced taqman probe based amplification
US20100279295A1 (en) * 2009-03-18 2010-11-04 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
WO2010135310A1 (en) * 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
US9914963B2 (en) 2009-09-28 2018-03-13 Igor Kutyavin Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes
WO2011087707A1 (en) 2009-12-22 2011-07-21 Elitech Holding B.V. Hypertheromostable endonuclease iv substrate probe
EP3202918B1 (en) 2011-04-07 2021-10-20 Abbott Diagnostics Scarborough, Inc. Monitoring recombinase polymerase amplification mixtures
CA2833172C (en) 2011-04-18 2020-06-02 Johnathan Charles Sharpe Marked straws and methods for marking straws
US8377657B1 (en) 2012-05-15 2013-02-19 Predictive Biosciences Corporation Primers for analyzing methylated sequences and methods of use thereof
AU2013264941B2 (en) * 2012-05-21 2016-12-22 Src, Inc. Methods and systems for the detection of ricin and other ribosome inactivating proteins
US20140255928A1 (en) 2013-03-11 2014-09-11 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
EP3230468B1 (en) 2014-12-12 2020-09-16 ELITechGroup, Inc. Methods and kits for detecting antibiotic resistant bacteria
US10266903B2 (en) 2014-12-12 2019-04-23 Elitechgroup, Inc. Methods and compositions for detecting antibiotic resistant bacteria
ES2909351T3 (es) 2017-08-17 2022-05-06 Elitechgroup Inc Quencheres estabilizadores de fluorescencia dúplex para sondas de ácidos nucleicos
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2022165353A1 (en) * 2021-02-01 2022-08-04 Abbott Laboratories Sequence conversion and signal amplifier dna having abasic nucleic acids, and detection methods using same
WO2024059788A2 (en) * 2022-09-15 2024-03-21 Chen cheng yao Method, kit and system for end labeling of nucleic acids

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5241060A (en) * 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5011769A (en) * 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
US5403711A (en) * 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
US5516663A (en) 1990-01-26 1996-05-14 Abbott Laboratories Ligase chain reaction with endonuclease IV correction and contamination control
EP0439182B1 (en) * 1990-01-26 1996-04-24 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US6582908B2 (en) * 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5574142A (en) * 1992-12-15 1996-11-12 Microprobe Corporation Peptide linkers for improved oligonucleotide delivery
WO1995005480A2 (en) * 1993-08-18 1995-02-23 Id Biomedical Corporation Compositions and methods for detecting target nucleic acid sequences utilizing flanking sequence enzyme molecules
US5426039A (en) * 1993-09-08 1995-06-20 Bio-Rad Laboratories, Inc. Direct molecular cloning of primer extended DNA containing an alkane diol
WO1995014106A2 (en) * 1993-11-17 1995-05-26 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
US6001610A (en) * 1994-11-23 1999-12-14 Roche Diagnostics, Gmbh Method for the particularly sensitive detection of nucleic acids
JPH08238100A (ja) * 1995-03-03 1996-09-17 Eiken Chem Co Ltd 所定の配列を有する核酸を特異的に検出する方法、並びに該方法に使用されるプローブ及びキット
US5656430A (en) * 1995-06-07 1997-08-12 Trevigen, Inc. Oscillating signal amplifier for nucleic acid detection
US5763178A (en) * 1995-06-07 1998-06-09 Trevigen, Inc. Oscillating signal amplifier for nucleic acid detection
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
US5747255A (en) * 1995-09-29 1998-05-05 Lynx Therapeutics, Inc. Polynucleotide detection by isothermal amplification using cleavable oligonucleotides
CA2258570A1 (en) * 1996-06-17 1997-12-24 Biodynamics Associates Method and kits for preparing multicomponent nucleic acid constructs
FR2763600B1 (fr) * 1997-05-20 1999-11-12 Genolife Procede de detection qualitative et quantitative d'alterations de l'adn et des ligands de ces alterations
US6274316B1 (en) * 1997-07-03 2001-08-14 Id Biomedical Corporation Compositions and methods for detecting vancomycin resistant enterococci by cycling probe reactions
EP1012342A4 (en) 1997-09-10 2004-07-21 Univ Maryland METHOD FOR THE AMPLIFICATION OF DNA AND RNA PRODUCTS, STARTING FROM SPLIT PIECES DUE TO MISSING BASE PAIRINGS
ATE469982T1 (de) * 1998-02-04 2010-06-15 Life Technologies Corp Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen
US5952202A (en) * 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
ES2172887T3 (es) 1998-04-22 2002-10-01 Entpr Ie Trd As Bioresearch Ie Un metodo para caracterizar moleculas de acido nucleico que implica generar fragmentos de dna aguas arriba y extensibles que resultan de la escision de un acido nucleico en un sitio abasico.
US6716971B1 (en) * 1998-09-08 2004-04-06 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Pteridine nucleotide analogs
US6268147B1 (en) * 1998-11-02 2001-07-31 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
US6579680B2 (en) * 2000-02-28 2003-06-17 Corning Incorporated Method for label-free detection of hybridized DNA targets
US6340566B1 (en) * 2000-03-28 2002-01-22 The Regents Of The University Of California Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
IE20000887A1 (en) * 2000-11-03 2002-12-11 Univ College Cork Nat Univ Ie Method for the amplification and optional characterisation of nucleic acids
WO2004018626A2 (en) * 2002-08-21 2004-03-04 Epoch Biosciences, Inc. Abasic site endonuclease assay
US20070020639A1 (en) * 2005-07-20 2007-01-25 Affymetrix, Inc. Isothermal locus specific amplification

Also Published As

Publication number Publication date
US7252940B2 (en) 2007-08-07
US7553643B2 (en) 2009-06-30
US7790385B2 (en) 2010-09-07
US20040101893A1 (en) 2004-05-27
JP2005536210A (ja) 2005-12-02
US20080166782A1 (en) 2008-07-10
WO2004018626A3 (en) 2004-05-27
WO2004018626A2 (en) 2004-03-04
CA2494993A1 (en) 2004-03-04
AU2003274914A1 (en) 2004-03-11
US20070141586A1 (en) 2007-06-21
EP1543154A4 (en) 2006-08-16
EP1543154A2 (en) 2005-06-22

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