CA2494993A1 - Abasic site endonuclease assay - Google Patents

Abasic site endonuclease assay Download PDF

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Publication number
CA2494993A1
CA2494993A1 CA002494993A CA2494993A CA2494993A1 CA 2494993 A1 CA2494993 A1 CA 2494993A1 CA 002494993 A CA002494993 A CA 002494993A CA 2494993 A CA2494993 A CA 2494993A CA 2494993 A1 CA2494993 A1 CA 2494993A1
Authority
CA
Canada
Prior art keywords
probe
nucleic acid
endonuclease
target nucleic
site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002494993A
Other languages
English (en)
French (fr)
Inventor
Igor V. Kutyavin
David Milesi
Merl Hoekstra
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epoch Biosciences Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2494993A1 publication Critical patent/CA2494993A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA002494993A 2002-08-21 2003-08-20 Abasic site endonuclease assay Abandoned CA2494993A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40564202P 2002-08-21 2002-08-21
US60/405,642 2002-08-21
PCT/US2003/026133 WO2004018626A2 (en) 2002-08-21 2003-08-20 Abasic site endonuclease assay

Publications (1)

Publication Number Publication Date
CA2494993A1 true CA2494993A1 (en) 2004-03-04

Family

ID=31946911

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002494993A Abandoned CA2494993A1 (en) 2002-08-21 2003-08-20 Abasic site endonuclease assay

Country Status (6)

Country Link
US (3) US7252940B2 (enExample)
EP (1) EP1543154A4 (enExample)
JP (1) JP4510626B2 (enExample)
AU (1) AU2003274914A1 (enExample)
CA (1) CA2494993A1 (enExample)
WO (1) WO2004018626A2 (enExample)

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US20110171649A1 (en) * 2008-09-10 2011-07-14 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
US8129149B1 (en) 2008-11-12 2012-03-06 The United States Of America As Represented By The Secretary Of The Army Rapid and sensitive method to measure endonuclease activity
US20110300544A1 (en) * 2008-12-09 2011-12-08 Guoliang Fu Enhanced taqman probe based amplification
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JP5847076B2 (ja) * 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Dnaグリコシラーゼ/リアーゼおよびapエンドヌクレアーゼ基質
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US8377657B1 (en) 2012-05-15 2013-02-19 Predictive Biosciences Corporation Primers for analyzing methylated sequences and methods of use thereof
US10907193B2 (en) * 2012-05-21 2021-02-02 Acumen Detection, Llc Methods and systems for the detection of ricin and other ribosome inactivating proteins
WO2014164479A1 (en) 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
EP3230467A1 (en) 2014-12-12 2017-10-18 ELITechGroup B.V. Methods and compositions for detecting antibiotic resistant bacteria
EP3230468B1 (en) 2014-12-12 2020-09-16 ELITechGroup, Inc. Methods and kits for detecting antibiotic resistant bacteria
US10677728B2 (en) 2017-08-17 2020-06-09 Elitechgroup B.V. Duplex stabilizing fluorescence quenchers for nucleic acid probes
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
JP2024506277A (ja) * 2021-02-01 2024-02-13 アボット・ラボラトリーズ 脱塩基核酸を有する配列変換及びシグナル増幅dna、並びにそれを使用する検出方法
CN116990266B (zh) * 2022-04-25 2026-07-17 北京大学 检测脱碱基核酸内切酶1的复合物荧光探针及其制备和应用
TWI872707B (zh) * 2022-09-15 2025-02-11 呈堯 陳 用於核酸末端標記的方法、套組和系統

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Also Published As

Publication number Publication date
EP1543154A4 (en) 2006-08-16
US7790385B2 (en) 2010-09-07
US20040101893A1 (en) 2004-05-27
US7252940B2 (en) 2007-08-07
US20080166782A1 (en) 2008-07-10
US7553643B2 (en) 2009-06-30
JP2005536210A (ja) 2005-12-02
WO2004018626A2 (en) 2004-03-04
JP4510626B2 (ja) 2010-07-28
WO2004018626A3 (en) 2004-05-27
EP1543154A2 (en) 2005-06-22
AU2003274914A1 (en) 2004-03-11
US20070141586A1 (en) 2007-06-21

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Legal Events

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EEER Examination request
FZDE Discontinued