JP4444564B2 - 三重螺旋相互作用によるターゲット二本鎖dna配列の精製及び検出方法 - Google Patents
三重螺旋相互作用によるターゲット二本鎖dna配列の精製及び検出方法 Download PDFInfo
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- JP4444564B2 JP4444564B2 JP2002575316A JP2002575316A JP4444564B2 JP 4444564 B2 JP4444564 B2 JP 4444564B2 JP 2002575316 A JP2002575316 A JP 2002575316A JP 2002575316 A JP2002575316 A JP 2002575316A JP 4444564 B2 JP4444564 B2 JP 4444564B2
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- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 229920003213 poly(N-isopropyl acrylamide) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229940096913 pseudoisocytidine Drugs 0.000 description 1
- IGFXRKMLLMBKSA-UHFFFAOYSA-N purine Chemical compound N1=C[N]C2=NC=NC2=C1 IGFXRKMLLMBKSA-UHFFFAOYSA-N 0.000 description 1
- 125000000561 purinyl group Chemical group N1=C(N=C2N=CNC2=C1)* 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000006268 reductive amination reaction Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 208000037803 restenosis Diseases 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 150000003440 styrenes Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 150000007970 thio esters Chemical class 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0103953A FR2822476B1 (fr) | 2001-03-23 | 2001-03-23 | Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice |
US28527201P | 2001-04-23 | 2001-04-23 | |
PCT/FR2002/001034 WO2002077274A2 (fr) | 2001-03-23 | 2002-03-25 | Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice. |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2004523245A JP2004523245A (ja) | 2004-08-05 |
JP4444564B2 true JP4444564B2 (ja) | 2010-03-31 |
Family
ID=26212934
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2002575316A Expired - Fee Related JP4444564B2 (ja) | 2001-03-23 | 2002-03-25 | 三重螺旋相互作用によるターゲット二本鎖dna配列の精製及び検出方法 |
Country Status (13)
Country | Link |
---|---|
EP (1) | EP1370692A2 (hu) |
JP (1) | JP4444564B2 (hu) |
KR (2) | KR101002499B1 (hu) |
CN (1) | CN1498276A (hu) |
BR (1) | BR0208292A (hu) |
CA (1) | CA2440133A1 (hu) |
HU (1) | HUP0303632A3 (hu) |
IL (2) | IL158017A0 (hu) |
MX (1) | MXPA03008642A (hu) |
NO (1) | NO20034184L (hu) |
NZ (1) | NZ528507A (hu) |
PL (1) | PL365208A1 (hu) |
WO (1) | WO2002077274A2 (hu) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1737945B1 (en) | 2004-04-19 | 2011-01-26 | Aventis Pharma S.A. | Method for purifying plasmid dna |
JP5770737B2 (ja) | 2009-11-06 | 2015-08-26 | ザ チャイニーズ ユニバーシティ オブ ホンコン | サイズに基づくゲノム分析 |
ES2399502T3 (es) * | 2010-07-13 | 2013-04-01 | Cnrs | Procedimiento para el aislamiento de las proteínas unidas a cualquier tipo de secuencia de ácido nucleico de interés |
US9892230B2 (en) | 2012-03-08 | 2018-02-13 | The Chinese University Of Hong Kong | Size-based analysis of fetal or tumor DNA fraction in plasma |
MX2020002124A (es) | 2017-08-25 | 2020-07-20 | Zoetis Services Llc | Un metodo para inmovilizar una sonda de acido nucleico a un soporte solido. |
CN109959691B (zh) * | 2019-04-15 | 2021-06-08 | 济南大学 | 一种基于级联光电活性材料和三螺旋分子开关检测核酸的方法 |
CN116396963B (zh) * | 2023-04-12 | 2023-10-13 | 南通大学 | 一类抑制血管内皮生长因子a表达的三链形成寡核苷酸及其应用 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0566670A4 (en) * | 1990-12-17 | 1993-12-08 | Idexx Laboratories, Inc. | Nucleic acid sequence detection by triple helix formation |
FR2728264B1 (fr) * | 1994-12-16 | 1997-01-31 | Rhone Poulenc Rorer Sa | Purification d'adn par formation de triple helice avec un oligonucleotide immobilise |
FR2731014B1 (fr) * | 1995-02-23 | 1997-03-28 | Rhone Poulenc Rorer Sa | Molecules d'adn, preparation et utilisation en therapie genique |
FR2746412B1 (fr) * | 1996-03-21 | 1998-06-12 | Rhone Poulenc Rorer Sa | Purification d'adn plasmidique de qualite pharmaceutique |
FR2776669B1 (fr) * | 1998-03-24 | 2001-06-15 | Rhone Poulenc Rorer Sa | Vecteurs de transfert d'acides nucleiques, compositions les contenant, et leurs utilisations |
-
2002
- 2002-03-25 JP JP2002575316A patent/JP4444564B2/ja not_active Expired - Fee Related
- 2002-03-25 CA CA002440133A patent/CA2440133A1/fr not_active Abandoned
- 2002-03-25 KR KR1020097016658A patent/KR101002499B1/ko not_active IP Right Cessation
- 2002-03-25 PL PL02365208A patent/PL365208A1/xx not_active Application Discontinuation
- 2002-03-25 HU HU0303632A patent/HUP0303632A3/hu unknown
- 2002-03-25 EP EP02722374A patent/EP1370692A2/fr not_active Withdrawn
- 2002-03-25 MX MXPA03008642A patent/MXPA03008642A/es active IP Right Grant
- 2002-03-25 WO PCT/FR2002/001034 patent/WO2002077274A2/fr active Application Filing
- 2002-03-25 IL IL15801702A patent/IL158017A0/xx unknown
- 2002-03-25 KR KR10-2003-7012346A patent/KR20030088464A/ko active IP Right Grant
- 2002-03-25 BR BR0208292-6A patent/BR0208292A/pt not_active Application Discontinuation
- 2002-03-25 CN CNA028070631A patent/CN1498276A/zh active Pending
- 2002-03-25 NZ NZ528507A patent/NZ528507A/en unknown
-
2003
- 2003-09-18 IL IL158017A patent/IL158017A/en not_active IP Right Cessation
- 2003-09-19 NO NO20034184A patent/NO20034184L/no unknown
Also Published As
Publication number | Publication date |
---|---|
WO2002077274A2 (fr) | 2002-10-03 |
NO20034184L (no) | 2003-11-04 |
WO2002077274A3 (fr) | 2003-10-02 |
MXPA03008642A (es) | 2004-06-30 |
NO20034184D0 (no) | 2003-09-19 |
AU2002253261C1 (en) | 2002-10-08 |
IL158017A (en) | 2011-07-31 |
PL365208A1 (en) | 2004-12-27 |
AU2002253261C9 (en) | 2002-10-08 |
JP2004523245A (ja) | 2004-08-05 |
HUP0303632A2 (hu) | 2004-01-28 |
IL158017A0 (en) | 2004-03-28 |
CA2440133A1 (fr) | 2002-10-03 |
KR20030088464A (ko) | 2003-11-19 |
BR0208292A (pt) | 2004-04-13 |
CN1498276A (zh) | 2004-05-19 |
KR101002499B1 (ko) | 2010-12-17 |
HUP0303632A3 (en) | 2005-12-28 |
EP1370692A2 (fr) | 2003-12-17 |
KR20090094870A (ko) | 2009-09-08 |
NZ528507A (en) | 2005-08-26 |
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