JP4441115B2 - ヒト非自己間葉幹細胞を使用する方法と利用 - Google Patents
ヒト非自己間葉幹細胞を使用する方法と利用 Download PDFInfo
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- JP4441115B2 JP4441115B2 JP2000535733A JP2000535733A JP4441115B2 JP 4441115 B2 JP4441115 B2 JP 4441115B2 JP 2000535733 A JP2000535733 A JP 2000535733A JP 2000535733 A JP2000535733 A JP 2000535733A JP 4441115 B2 JP4441115 B2 JP 4441115B2
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- stem cells
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3912798A | 1998-03-13 | 1998-03-13 | |
| US09/039,127 | 1998-03-13 | ||
| PCT/US1999/005351 WO1999046366A1 (en) | 1998-03-13 | 1999-03-12 | Uses for humane non-autologous mesenchymal stem cells |
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| Publication Number | Publication Date |
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| JP2002506082A JP2002506082A (ja) | 2002-02-26 |
| JP2002506082A5 JP2002506082A5 (https=) | 2006-04-27 |
| JP4441115B2 true JP4441115B2 (ja) | 2010-03-31 |
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| JP2000535733A Expired - Lifetime JP4441115B2 (ja) | 1998-03-13 | 1999-03-12 | ヒト非自己間葉幹細胞を使用する方法と利用 |
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| US (2) | US6355239B1 (https=) |
| EP (1) | EP1062321B1 (https=) |
| JP (1) | JP4441115B2 (https=) |
| AT (1) | ATE286118T1 (https=) |
| AU (1) | AU749675B2 (https=) |
| CA (1) | CA2323073C (https=) |
| DE (1) | DE69922933T2 (https=) |
| ES (1) | ES2237089T3 (https=) |
| PT (1) | PT1062321E (https=) |
| WO (1) | WO1999046366A1 (https=) |
Families Citing this family (238)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7767452B2 (en) * | 1997-02-20 | 2010-08-03 | Kleinsek Don A | Tissue treatments with adipocyte cells |
| AU749675B2 (en) * | 1998-03-13 | 2002-07-04 | Mesoblast International Sarl | Uses for human non-autologous mesenchymal stem cells |
| US6866842B1 (en) | 1998-05-01 | 2005-03-15 | University Of Pittsburgh | Muscle-derived cells (MDCs) for treating muscle-or bone-related injury or dysfunction |
| US7115417B1 (en) | 1998-05-01 | 2006-10-03 | Chancellor Michael B | Soft tissue and bone augmentation and bulking utilizing muscle-derived progenito compositions, and treatments thereof |
| AU766064B2 (en) * | 1998-10-26 | 2003-10-09 | Philadelphia Health & Education Corporation | Stromal cell use |
| CA2348514A1 (en) * | 1998-11-13 | 2000-05-25 | Osiris Therapeutics, Inc. | In utero transplantation of human mesenchymal stem cells |
| AU2998300A (en) * | 1999-02-17 | 2000-09-04 | United States Surgical Corporation | Genetically altered mesenchymal stem cells and methods of use thereof |
| US8147824B2 (en) * | 1999-08-05 | 2012-04-03 | Athersys, Inc. | Immunomodulatory properties of multipotent adult progenitor cells and uses thereof |
| US20080267923A2 (en) * | 1999-11-05 | 2008-10-30 | Donald Kleinsek | Hair undifferentiated cells |
| US20090074729A2 (en) * | 1999-11-05 | 2009-03-19 | Donald Kleinsek | Augmentation and repair of spincter defects with cells including fibroblasts |
| US7799325B2 (en) * | 1999-11-05 | 2010-09-21 | Kleinsek Donald A | Removal of hypertrophic scars |
| US20080286242A2 (en) * | 1999-11-05 | 2008-11-20 | Donald Kleinsek | Augmentation and repair of spincter defects with cells including mesenchymal cells |
| ES2288946T3 (es) * | 2000-04-14 | 2008-02-01 | University Of Pittsburgh | Aumento y abultamiento de tejido blando y hueso utilizando celulas progenitoras derivadas de musculo, sus composiciones y tratamientos. |
| DE60143517D1 (de) | 2000-04-25 | 2011-01-05 | Osiris Therapeutics Inc | Wiederherstellung der gelenken mit mesenchymalen stammzellen |
| AU2006200478B2 (en) * | 2000-04-25 | 2007-08-02 | Mesoblast International Sarl | Joint repair using mesenchymal stem cells |
| CA2411101C (en) | 2000-06-05 | 2018-01-16 | Brigham And Women's Hospital, Inc. | A gene encoding a multidrug resistance human p-glycoprotein homologue on chromosome 7p15-21 and uses thereof |
| US20090130066A1 (en) * | 2000-11-06 | 2009-05-21 | Gerigene Medical Corporation | Augmentation and repair of sphincter defects with cells including muscle cells |
| WO2002040061A2 (en) * | 2000-11-15 | 2002-05-23 | Osiris Therapeutics, Inc. | Immunocompetent animals including xenogeneic implants of mesenchymal stem cells |
| US7311905B2 (en) * | 2002-02-13 | 2007-12-25 | Anthrogenesis Corporation | Embryonic-like stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells |
| EP2305795B1 (en) * | 2000-12-06 | 2019-07-03 | Celularity, Inc. | Method of collecting placental stem cells |
| US20080152629A1 (en) * | 2000-12-06 | 2008-06-26 | James Edinger | Placental stem cell populations |
| EP2316918B1 (en) * | 2001-02-14 | 2015-07-01 | Anthrogenesis Corporation | Post-partum mammalian placenta, its use and placental stem cells therefrom |
| ES2432493T3 (es) * | 2001-02-14 | 2013-12-03 | Anthrogenesis Corporation | Placenta de mamífero post-parto, su uso y células madre de la misma |
| WO2003047607A1 (fr) * | 2001-12-06 | 2003-06-12 | Sankyo Company, Limited | Compositions medicales contenant des cellules amniotiques humaines |
| US20050095228A1 (en) | 2001-12-07 | 2005-05-05 | Fraser John K. | Methods of using regenerative cells in the treatment of peripheral vascular disease and related disorders |
| US7651684B2 (en) | 2001-12-07 | 2010-01-26 | Cytori Therapeutics, Inc. | Methods of using adipose tissue-derived cells in augmenting autologous fat transfer |
| US7585670B2 (en) | 2001-12-07 | 2009-09-08 | Cytori Therapeutics, Inc. | Automated methods for isolating and using clinically safe adipose derived regenerative cells |
| US7771716B2 (en) * | 2001-12-07 | 2010-08-10 | Cytori Therapeutics, Inc. | Methods of using regenerative cells in the treatment of musculoskeletal disorders |
| US9597395B2 (en) | 2001-12-07 | 2017-03-21 | Cytori Therapeutics, Inc. | Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions |
| US7595043B2 (en) * | 2001-12-07 | 2009-09-29 | Cytori Therapeutics, Inc. | Method for processing and using adipose-derived stem cells |
| JP4653952B2 (ja) | 2001-12-07 | 2011-03-16 | サイトリ セラピューティクス インコーポレイテッド | 処理済み脂肪吸引細胞で患者を治療するためのシステムと方法 |
| US20050048035A1 (en) | 2001-12-07 | 2005-03-03 | Fraser John K. | Methods of using regenerative cells in the treatment of stroke and related diseases and disorders |
| WO2003073998A2 (en) * | 2002-03-02 | 2003-09-12 | Board Of Regents, The University Of Texas System | Local production and/or delivery of anti-cancer agents by stromal cell precursors |
| US20030205538A1 (en) | 2002-05-03 | 2003-11-06 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
| US7992725B2 (en) * | 2002-05-03 | 2011-08-09 | Biomet Biologics, Llc | Buoy suspension fractionation system |
| US7832566B2 (en) | 2002-05-24 | 2010-11-16 | Biomet Biologics, Llc | Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles |
| DE10392686T5 (de) | 2002-05-24 | 2005-07-07 | Biomet Mfg. Corp., Warsaw | Vorrichtung und Verfahren zum Trennen und Konzentrieren von Flüssigkeiten, welche mehrere Komponenten enthalten |
| US7845499B2 (en) | 2002-05-24 | 2010-12-07 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| US20060278588A1 (en) * | 2002-05-24 | 2006-12-14 | Woodell-May Jennifer E | Apparatus and method for separating and concentrating fluids containing multiple components |
| US20040033217A1 (en) * | 2002-05-31 | 2004-02-19 | Padmavathy Vanguri | Intraperitoneal delivery of genetically engineered mesenchymal stem cells |
| EP1545219A4 (en) * | 2002-07-23 | 2009-09-30 | Boston Scient Ltd | CELL THERAPY FOR REGENERATION |
| AR047712A1 (es) * | 2002-09-07 | 2006-02-15 | Royal Veterinary College | Metodo de tratamiento de una lesion de tejido esqueletico blando natural administrando una composicion de celulas madre mesenquimatosas |
| AU2003272980A1 (en) * | 2002-10-28 | 2004-05-13 | Nsk Ltd. | Lubricating grease composition for deceleration gear and electric power steering |
| AU2003280824A1 (en) * | 2002-11-15 | 2004-06-15 | Fumihiko Ishikawa | Method of organ regeneration |
| AU2003298775B2 (en) * | 2002-11-26 | 2008-07-17 | Anthrogenesis Corporation | Cytotherapeutics, cytotherapeutic units and methods for treatments using them |
| US7470538B2 (en) | 2002-12-05 | 2008-12-30 | Case Western Reserve University | Cell-based therapies for ischemia |
| US20050048041A1 (en) * | 2003-01-13 | 2005-03-03 | Rao Mahendra S. | Persistent expression of candidate molecule in proliferating stem and progenitor cells for delivery of therapeutic products |
| US20040197310A1 (en) * | 2003-02-12 | 2004-10-07 | Sanberg Paul R. | Compositions and methods for using umbilical cord progenitor cells in the treatment of myocardial infarction |
| CN1770976A (zh) * | 2003-02-13 | 2006-05-10 | 人类起源公司 | 利用脐带血治疗患有疾病、紊乱或状况的个体的用途 |
| ATE482725T1 (de) * | 2003-04-01 | 2010-10-15 | Us Dept Of Veteran S Affaires | Auf stammzellen, vorläuferzellen oder targetzellen basierende behandlung von multiorganversagen. |
| ATE482713T1 (de) | 2003-04-25 | 2010-10-15 | Univ Pittsburgh | Von muskeln stammende zellen (mdcs) zur förderung und verbesserung der nervenreparatur und - regeneration |
| US7875272B2 (en) | 2003-06-27 | 2011-01-25 | Ethicon, Incorporated | Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells |
| EP2399990B1 (en) | 2003-06-27 | 2015-07-22 | DePuy Synthes Products, Inc. | Cells derived from post-partum umbilical cord for use in treatment of disease of the heart and circulatory system |
| US8790637B2 (en) | 2003-06-27 | 2014-07-29 | DePuy Synthes Products, LLC | Repair and regeneration of ocular tissue using postpartum-derived cells |
| US9572840B2 (en) | 2003-06-27 | 2017-02-21 | DePuy Synthes Products, Inc. | Regeneration and repair of neural tissue using postpartum-derived cells |
| US8518390B2 (en) | 2003-06-27 | 2013-08-27 | Advanced Technologies And Regenerative Medicine, Llc | Treatment of stroke and other acute neural degenerative disorders via intranasal administration of umbilical cord-derived cells |
| US8491883B2 (en) * | 2003-06-27 | 2013-07-23 | Advanced Technologies And Regenerative Medicine, Llc | Treatment of amyotrophic lateral sclerosis using umbilical derived cells |
| US9592258B2 (en) | 2003-06-27 | 2017-03-14 | DePuy Synthes Products, Inc. | Treatment of neurological injury by administration of human umbilical cord tissue-derived cells |
| US7744869B2 (en) * | 2003-08-20 | 2010-06-29 | Ebi, Llc | Methods of treatment using electromagnetic field stimulated mesenchymal stem cells |
| GB0321337D0 (en) * | 2003-09-11 | 2003-10-15 | Massone Mobile Advertising Sys | Method and system for distributing advertisements |
| JP2007508018A (ja) | 2003-10-08 | 2007-04-05 | べト−ステム インコーポレイテッド | 新規の幹細胞組成物の調製法および使用法、ならびにこの組成物を含有するキット |
| AU2004279246A1 (en) * | 2003-10-14 | 2005-04-21 | Chugai Seiyaku Kabushiki Kaisha | Remedy for kidney diseases |
| US20070269412A1 (en) | 2003-12-02 | 2007-11-22 | Celavie Biosciences, Llc | Pluripotent cells |
| KR20120039065A (ko) * | 2003-12-02 | 2012-04-24 | 셀진 코포레이션 | 혈색소병증 및 빈혈의 치료 및 관리를 위한 방법및 조성물 |
| US20080095749A1 (en) | 2004-03-22 | 2008-04-24 | Sudeepta Aggarwal | Mesenchymal stem cells and uses therefor |
| EP2298864B1 (en) * | 2004-03-22 | 2017-10-11 | Mesoblast International Sàrl | Mesenchymal stem cells and uses therefor |
| US7622108B2 (en) * | 2004-04-23 | 2009-11-24 | Bioe, Inc. | Multi-lineage progenitor cells |
| WO2005108559A2 (en) * | 2004-04-23 | 2005-11-17 | Bioe, Inc. | Multi-lineage progenitor cells |
| KR100593397B1 (ko) | 2004-10-27 | 2006-06-28 | 한국원자력연구소 | 중배엽 줄기세포 및/또는 p 물질을 함유하는 상처 치유또는 상처 치유 촉진제, 또는 세포 치료제 |
| US20060111778A1 (en) * | 2004-10-29 | 2006-05-25 | Michalow Alexander E | Methods of promoting healing of cartilage defects and method of causing stem cells to differentiate by the articular chondrocyte pathway |
| EP1851858B1 (en) * | 2004-12-03 | 2016-06-08 | New Jersey Institute of Technology | Substrate recognition by differentiable human mesenchymal stem cells |
| US20060153815A1 (en) * | 2004-12-21 | 2006-07-13 | Agnieszka Seyda | Tissue engineering devices for the repair and regeneration of tissue |
| US20060171930A1 (en) * | 2004-12-21 | 2006-08-03 | Agnieszka Seyda | Postpartum cells derived from umbilical cord tissue, and methods of making, culturing, and using the same |
| US20060166361A1 (en) * | 2004-12-21 | 2006-07-27 | Agnieszka Seyda | Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same |
| EP1835924B1 (en) | 2004-12-23 | 2013-08-21 | Ethicon, Incorporated | Treatment of parkinson's disease and related disorders using postpartum derived cells |
| CA2589041C (en) | 2004-12-23 | 2019-08-20 | DePuy Synthes Products, Inc. | Postpartum cells derived from umbilical cord tissue, and methods of making and using the same |
| CA2512667A1 (en) * | 2005-01-07 | 2006-07-07 | Takahiro Ochiya | Human hepatocyte-like cells and uses thereof |
| AU2006205033A1 (en) * | 2005-01-11 | 2006-07-20 | Cognate Therapeutics, Inc. | Bone marrow stromal cells for immunoprotection of transplanted neural stem cells |
| EP1860180A4 (en) * | 2005-02-23 | 2009-03-11 | Found Biomedical Res & Innov | METHOD FOR THE AMPLIFICATION OF AN ENDOTHEL PREPARATION CELL IN VITRO |
| JP2006289062A (ja) * | 2005-03-18 | 2006-10-26 | Pentax Corp | 肥大化能を有する軟骨細胞と足場を用いた骨補填材料 |
| JP2008544957A (ja) * | 2005-05-10 | 2008-12-11 | ユナイテッド ステイツ オブ アメリカ デパートメント オブ ヴェテランズ アフェアーズ | 間充織幹細胞による腎疾患及び多臓器不全の治療法と間充織幹細胞馴化培地 |
| JP2006345726A (ja) * | 2005-06-14 | 2006-12-28 | Hamamatsu Univ School Of Medicine | 脳腫瘍治療用発現ベクター |
| BRPI0617085A2 (pt) * | 2005-09-02 | 2016-11-08 | Agency Science Tech & Res | método para obter uma célula-tronco mesenquimal (msc), célula ou linhagem celular, método para derivar uma cultura celular a partir de uma célula-tronco embrionária, método para tratamento de uma doença, uso de uma célula-tronco mesenquimal, linhagem diferenciada, composição farmacêutica, método para condicionar um meio de cultura celular, meio condicionado e uso do mesmo, mpetodo para obterum polipeptídeo, método para obtar uma cultura celular, cultura celular, célula-tronco mesenquimal, linhagem de célula-tronco mesenquimal ou uma célula mesenquimal diferenciada |
| US20070065415A1 (en) * | 2005-09-16 | 2007-03-22 | Kleinsek Donald A | Compositions and methods for the augmentation and repair of defects in tissue |
| WO2007035843A2 (en) * | 2005-09-21 | 2007-03-29 | Dask Technologies, Llc | Methods and compositions for organ and tissue functionality |
| NZ597304A (en) | 2005-10-13 | 2013-06-28 | Anthrogenesis Corp | Immunomodulation using placental stem cells |
| US20070105769A1 (en) * | 2005-11-07 | 2007-05-10 | Ebi, L.P. | Methods of treating tissue defects |
| US11000546B2 (en) | 2005-11-09 | 2021-05-11 | Athersys, Inc. | Immunomodulatory properties of MAPCs and uses thereof |
| US10117900B2 (en) | 2005-11-09 | 2018-11-06 | Athersys, Inc. | MAPC treatment of brain injuries and diseases |
| WO2007070870A1 (en) * | 2005-12-16 | 2007-06-21 | Ethicon, Inc. | Compositions and methods for inhibiting adverse immune response in histocompatibility-mismatched transplantation |
| US8741638B2 (en) * | 2005-12-19 | 2014-06-03 | DePuy Synthes Products, LLC | In vitro expansion of postpartum-derived cells in roller bottles |
| RU2301667C1 (ru) * | 2005-12-26 | 2007-06-27 | Общество с ограниченной ответственностью "1.91 РАД" (ООО "1.91 РАД") | Способ лечения заболеваний различной этиологии и травм различных органов |
| US9125906B2 (en) | 2005-12-28 | 2015-09-08 | DePuy Synthes Products, Inc. | Treatment of peripheral vascular disease using umbilical cord tissue-derived cells |
| EP1979050B1 (en) * | 2005-12-28 | 2017-04-19 | DePuy Synthes Products, Inc. | Treatment of peripheral vascular disease using postpartum-derived cells |
| EP1976978A2 (en) | 2005-12-29 | 2008-10-08 | Anthrogenesis Corporation | Co-culture of placental stem cells and stem cells from a second source |
| PL2471907T3 (pl) | 2005-12-29 | 2019-07-31 | Celularity, Inc. | Populacje komórek macierzystych łożyska |
| US20070253931A1 (en) * | 2006-01-12 | 2007-11-01 | Osiris Therapeutics, Inc. | Use of mesenchymal stem cells for treating genetic diseases and disorders |
| US20080286249A1 (en) * | 2006-01-12 | 2008-11-20 | Varney Timothy R | Use of mesenchymal stem cells for treating genetic diseases and disorders |
| US11992507B2 (en) | 2006-01-23 | 2024-05-28 | Abt Holding Company | MAPC therapeutics without adjunctive immunosuppressive treatment |
| US20110171182A1 (en) * | 2006-03-23 | 2011-07-14 | Pluristem Ltd. | Methods for cell expansion and uses of cells and conditioned media produced thereby for therapy |
| US8298528B2 (en) * | 2006-04-17 | 2012-10-30 | Hepacore Ltd. | Methods for bone regeneration using endothelial progenitor cell preparations |
| CA2646491A1 (en) * | 2006-04-17 | 2007-10-25 | Bioe, Inc. | Differentiation of multi-lineage progenitor cells to respiratory epithelial cells |
| US8567609B2 (en) | 2006-05-25 | 2013-10-29 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| SI2772535T1 (sl) * | 2006-05-31 | 2022-08-31 | Children's Medical Center Corporation | ABC5 pozitivne mezenhimske matične celice kot imunomodulatorji |
| KR20090031895A (ko) * | 2006-06-09 | 2009-03-30 | 안트로제네시스 코포레이션 | 태반 니치 및 줄기 세포 배양을 위한 이의 용도 |
| US7993918B2 (en) * | 2006-08-04 | 2011-08-09 | Anthrogenesis Corporation | Tumor suppression using placental stem cells |
| US8562972B2 (en) | 2006-10-23 | 2013-10-22 | Anthrogenesis Corporation | Methods and compositions for treatment of bone defects with placental cell populations |
| US7871605B2 (en) | 2006-11-03 | 2011-01-18 | Aastrom Bioscience, Inc. | Mixed cell populations for tissue repair and separation technique for cell processing |
| WO2008085229A2 (en) * | 2006-11-15 | 2008-07-17 | Arteriocyte Inc. | Cell-based therapies for treating liver disease |
| EP2097088B2 (en) | 2006-11-28 | 2024-06-12 | University of Pittsburgh - of the Commonwealth System of Higher Education | Muscle derived cells for the treatment of cardiac pathologies and methods of making and using the same |
| US20100158875A1 (en) | 2006-12-18 | 2010-06-24 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Muscle derived cells for the treatment of gastro-esophageal pathologies and methods of making and using the same |
| WO2008076435A1 (en) | 2006-12-18 | 2008-06-26 | University Of Pittsburgh | Muscle derived cells for the treatment of gastro-esophageal pathologies and methods of making and using the same |
| DK2118271T3 (en) | 2007-01-11 | 2018-07-23 | Univ Pittsburgh Commonwealth Sys Higher Education | MUSCLE DERIVATIVE CELLS FOR THE TREATMENT OF URINARY DISEASES AND PROCEDURES FOR THEIR PREPARATION AND USE |
| US20080193424A1 (en) * | 2007-02-09 | 2008-08-14 | Biomet Biologics, Inc. | Treatment of tissue defects with a therapeutic composition |
| NZ612888A (en) | 2007-02-12 | 2015-02-27 | Anthrogenesis Corp | Treatment of inflammatory diseases using placental stem cells |
| US8034014B2 (en) | 2007-03-06 | 2011-10-11 | Biomet Biologics, Llc | Angiogenesis initation and growth |
| US20100172830A1 (en) * | 2007-03-29 | 2010-07-08 | Cellx Inc. | Extraembryonic Tissue cells and method of use thereof |
| WO2008127639A1 (en) | 2007-04-12 | 2008-10-23 | Biomet Biologics, Llc | Buoy suspension fractionation system |
| US8328024B2 (en) | 2007-04-12 | 2012-12-11 | Hanuman, Llc | Buoy suspension fractionation system |
| US20100291042A1 (en) | 2007-05-03 | 2010-11-18 | The Brigham And Women's Hospital, Inc. | Multipotent stem cells and uses thereof |
| TWM322542U (en) * | 2007-05-23 | 2007-11-21 | Universal Scient Ind Co Ltd | Testing machine |
| WO2008157594A2 (en) | 2007-06-18 | 2008-12-24 | New Jersey Institute Of Technology | Electrospun ceramic-polymer composite as a scaffold for tissue repair |
| CA2688504A1 (en) | 2007-06-18 | 2008-12-24 | Children's Hospital & Research Center At Oakland | Method of isolating stem and progenitor cells from placenta |
| EP2192908A4 (en) * | 2007-07-25 | 2010-09-01 | Bioe Inc | DIFFERENTIATION OF PRECURSOR CELLS OF MULTIPLE ABSTRACTS AGAINST CHONDROCYTES |
| KR20220122774A (ko) * | 2007-09-26 | 2022-09-02 | 셀룰래리티 인코포레이티드 | 인간 태반 관류액으로부터의 혈관형성 세포 |
| RU2536242C2 (ru) | 2007-09-28 | 2014-12-20 | Антродженезис Корпорейшн | Угнетение опухолей с помощью плацентарного перфузата человека и выделенных из плаценты человека вспомогательных натуральных клеток-киллеров |
| ES2525718T3 (es) * | 2007-10-05 | 2014-12-29 | DePuy Synthes Products, LLC | Reparación y regeneración de tejido renal mediante células derivadas de tejido de cordón umbilical humano |
| EP2217329B1 (en) * | 2007-11-07 | 2018-01-10 | Anthrogenesis Corporation | Use of umbilical cord blood in the treatment of premature birth complications |
| US8236538B2 (en) * | 2007-12-20 | 2012-08-07 | Advanced Technologies And Regenerative Medicine, Llc | Methods for sterilizing materials containing biologically active agents |
| UA99167C2 (ru) * | 2007-12-27 | 2012-07-25 | Этикон, Инкорпорейтед | Лечение дегенерации межреберных дисков с использованием клеток, полученных из ткани пуповины человека |
| US20090192528A1 (en) * | 2008-01-29 | 2009-07-30 | Biomet Biologics, Inc. | Method and device for hernia repair |
| EP2620139B1 (en) | 2008-02-27 | 2016-07-20 | Biomet Biologics, LLC | Interleukin-1 receptor antagonist rich solutions |
| WO2009111338A1 (en) * | 2008-02-29 | 2009-09-11 | Biomet Manufacturing Corp. | A system and process for separating a material |
| US9011840B2 (en) | 2008-03-14 | 2015-04-21 | The Board Of Trustees Of The University Of Illinois | Activated mesenchymal stem cells for wound healing and impaired tissue regeneration |
| US20110044958A1 (en) * | 2008-03-14 | 2011-02-24 | The Board Of Trustees Of The University Of Illinois | Activated mesenchymal stem cells for the prevention and repair of inflammatory states |
| US20090325296A1 (en) | 2008-03-25 | 2009-12-31 | New Jersey Institute Of Technology | Electrospun electroactive polymers for regenerative medicine applications |
| EP2279246A4 (en) * | 2008-05-02 | 2012-03-28 | Massachusetts Inst Technology | METHOD AND COMPOSITIONS FOR MODULATING IMMUNOLOGICAL TOLERANCE |
| CA2724839A1 (en) * | 2008-05-21 | 2009-11-26 | Bioe Llc | Differentiation of multi-lineage progenitor cells to pancreatic cells |
| WO2009142770A2 (en) * | 2008-05-23 | 2009-11-26 | The Regents Of The University Of California | Compositions and methods for generating musculoskeletal tissue |
| US9023819B2 (en) * | 2008-06-09 | 2015-05-05 | National Chung Cheng University | Treatment of a disease or a condition associated with aberrant gene hypomethylation by a method involving tailored epigenomic modification |
| US8067163B2 (en) * | 2008-06-09 | 2011-11-29 | National Chung Cheng University | Determination of the biological function of a target gene in a cell |
| EP2328596B1 (en) | 2008-08-18 | 2019-03-06 | University of Pittsburgh - Of the Commonwealth System of Higher Education | Bone augmentation utilizing muscle-derived progenitor compositions in biocompatible matrix, and treatments thereof |
| WO2010021993A1 (en) | 2008-08-19 | 2010-02-25 | Cytori Therapeutics, Inc. | Methods of using adipose tissue-derived cells in the treatment of the lymphatic system and malignant disease |
| CA2734237C (en) * | 2008-08-20 | 2019-07-02 | Anthrogenesis Corporation | Treatment of stroke using isolated placental cells |
| KR20200011604A (ko) * | 2008-08-20 | 2020-02-03 | 안트로제네시스 코포레이션 | 개선된 세포 조성물 및 그의 제조 방법 |
| CA2734446C (en) * | 2008-08-22 | 2017-06-20 | Anthrogenesis Corporation | Methods and compositions for treatment of bone defects with placental cell populations |
| EP3978928A1 (en) | 2008-11-14 | 2022-04-06 | The Brigham and Women's Hospital, Inc. | Therapeutic and diagnostic methods relating to cancer stem cells |
| US11542328B2 (en) | 2008-11-14 | 2023-01-03 | The Brigham And Women's Hospital, Inc. | Therapeutic and diagnostic methods relating to cancer stem cells |
| RU2015130665A (ru) * | 2008-11-19 | 2018-12-24 | Антродженезис Корпорейшн | Амниотические адгезивные клетки |
| US9192695B2 (en) | 2008-11-20 | 2015-11-24 | Allosource | Allografts combined with tissue derived stem cells for bone healing |
| DK2375907T3 (da) * | 2008-11-21 | 2019-06-11 | Celularity Inc | Behandling af sygdomme, lidelser eller tilstande i lungerne under anvendelse af placentaceller |
| US10098333B2 (en) * | 2008-12-09 | 2018-10-16 | University Of Southern California | Method for treating an SLE-like autoimmune disease in a human subject consisting of administering stem cells from human exfoliated deciduous teeth (SHED) and erythropoietin (EPO) to said human subject |
| EP2379087B1 (en) * | 2008-12-19 | 2014-08-20 | DePuy Synthes Products, LLC | Umbilical cord tissue derived cells for treating neuropathic pain and spasticity |
| CN102458425A (zh) * | 2008-12-19 | 2012-05-16 | 伊西康公司 | 损伤后神经组织的再生和修复 |
| US10179900B2 (en) * | 2008-12-19 | 2019-01-15 | DePuy Synthes Products, Inc. | Conditioned media and methods of making a conditioned media |
| JP5518893B2 (ja) | 2008-12-19 | 2014-06-11 | アドバンスト・テクノロジーズ・アンド・リジェネレイティブ・メディスン・エルエルシー | 肺ならびに肺の疾患および障害の治療 |
| US8771677B2 (en) * | 2008-12-29 | 2014-07-08 | Vladimir B Serikov | Colony-forming unit cell of human chorion and method to obtain and use thereof |
| US20100209399A1 (en) * | 2009-02-13 | 2010-08-19 | Celavie Biosciences, Llc | Brain-derived stem cells for repair of musculoskeletal system in vertebrate subjects |
| US8187475B2 (en) | 2009-03-06 | 2012-05-29 | Biomet Biologics, Llc | Method and apparatus for producing autologous thrombin |
| US9476026B2 (en) | 2009-03-12 | 2016-10-25 | New Jersey Institute Of Technology | Method of tissue repair using a piezoelectric scaffold |
| US9192655B2 (en) | 2009-03-12 | 2015-11-24 | New Jersey Institute Of Technology | System and method for a hydrogel and hydrogel composite for cartilage repair applications |
| US9771557B2 (en) | 2009-03-12 | 2017-09-26 | New Jersey Institute Of Technology | Piezoelectric scaffold for nerve growth and repair |
| US9334476B2 (en) | 2009-03-12 | 2016-05-10 | New Jersey Institute Of Technology | Method for nerve growth and repair using a piezoelectric scaffold |
| EP2411504B1 (en) | 2009-03-26 | 2017-05-10 | DePuy Synthes Products, Inc. | Human umbilical cord tissue cells as therapy for alzheimer's disease |
| US20100247495A1 (en) * | 2009-03-30 | 2010-09-30 | Tom Ichim | Treatment of Muscular Dystrophy |
| US8313954B2 (en) | 2009-04-03 | 2012-11-20 | Biomet Biologics, Llc | All-in-one means of separating blood components |
| KR101738324B1 (ko) * | 2009-05-01 | 2017-05-19 | 비미니 테크놀로지스 엘엘씨 | 조직 및 세포 부유화 이식편의 최적화 시스템, 방법 및 조성물 |
| US9301975B2 (en) | 2009-05-01 | 2016-04-05 | Biocardia, Inc. | Method of preparing autologous cells and method of use for therapy |
| US9011800B2 (en) | 2009-07-16 | 2015-04-21 | Biomet Biologics, Llc | Method and apparatus for separating biological materials |
| US8784803B2 (en) * | 2009-08-28 | 2014-07-22 | Osaka Air Machine Service, Ltd. | Therapeutic agent for liver-related diseases |
| WO2011064733A1 (en) | 2009-11-27 | 2011-06-03 | Stempeutics Research Pvt. Ltd. | Methods of preparing mesenchymal stem cells, compositions and kit thereof |
| US20140286911A1 (en) | 2013-03-15 | 2014-09-25 | Allosource | Cell repopulated collagen matrix for soft tissue repair and regeneration |
| DK3284818T3 (da) * | 2010-01-26 | 2022-06-20 | Celularity Inc | Behandling af knoglerelateret kræft ved hjælp af placenta stamceller |
| WO2011102890A1 (en) * | 2010-02-18 | 2011-08-25 | Albert Einstein College Of Medicine Of Yeshiva University | Methods of treatment of hemophilia |
| US9180166B2 (en) | 2010-03-12 | 2015-11-10 | New Jersey Institute Of Technology | Cartilage repair systems and applications utilizing a glycosaminoglycan mimic |
| DK2556145T3 (en) | 2010-04-07 | 2016-11-07 | Anthrogenesis Corp | Angiogenesis using placental stem cells |
| EP2555783A1 (en) | 2010-04-08 | 2013-02-13 | Anthrogenesis Corporation | Treatment of sarcoidosis using placental stem cells |
| US8591391B2 (en) | 2010-04-12 | 2013-11-26 | Biomet Biologics, Llc | Method and apparatus for separating a material |
| US10130736B1 (en) | 2010-05-14 | 2018-11-20 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US8883210B1 (en) | 2010-05-14 | 2014-11-11 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US9352003B1 (en) | 2010-05-14 | 2016-05-31 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| EP2576768B1 (en) | 2010-06-01 | 2017-05-10 | Auxocell Laboratories, Inc. | Native wharton's jelly stem cells and their purification |
| MX342995B (es) | 2010-07-13 | 2016-10-21 | Anthrogenesis Corp | Métodos de generar células asesinas naturales. |
| WO2012030968A1 (en) * | 2010-08-31 | 2012-03-08 | Renovocyte, LLC | Systemic, allogenic stem cell therapies for treatment of diseases in animals |
| US20120087933A1 (en) | 2010-10-08 | 2012-04-12 | Samson Tom | Enhanced msc preparations |
| EP2625264B1 (en) | 2010-10-08 | 2022-12-07 | Terumo BCT, Inc. | Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions |
| WO2012092485A1 (en) | 2010-12-31 | 2012-07-05 | Anthrogenesis Corporation | Enhancement of placental stem cell potency using modulatory rna molecules |
| KR101903339B1 (ko) | 2011-03-22 | 2018-10-01 | 플루리스템 리미티드 | 방사선 또는 화학적 손상을 치료하는 방법 |
| US10081794B2 (en) | 2011-04-13 | 2018-09-25 | New Jersey Institute Of Technology | System and method for electrospun biodegradable scaffold for bone repair |
| US8834928B1 (en) | 2011-05-16 | 2014-09-16 | Musculoskeletal Transplant Foundation | Tissue-derived tissugenic implants, and methods of fabricating and using same |
| ES2707579T3 (es) | 2011-06-01 | 2019-04-04 | Celularity Inc | Tratamiento del dolor usando citoblastos placentarios |
| WO2013055476A1 (en) | 2011-09-09 | 2013-04-18 | Anthrogenesis Corporation | Treatment of amyotrophic lateral sclerosis using placental stem cells |
| EP2794854B1 (en) | 2011-12-23 | 2018-06-20 | DePuy Synthes Products, Inc. | Detection of human umbilical cord tissue-derived cells |
| GB201202319D0 (en) | 2012-02-10 | 2012-03-28 | Orbsen Therapeutics Ltd | Stromal stem cells |
| US9642956B2 (en) | 2012-08-27 | 2017-05-09 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| AU2014215458A1 (en) | 2013-02-05 | 2015-08-13 | Anthrogenesis Corporation | Natural killer cells from placenta |
| WO2014130518A1 (en) | 2013-02-19 | 2014-08-28 | Children's Medical Center Corporation | Abcb5(+) stem cells for treating ocular disease |
| US9511119B2 (en) | 2013-03-15 | 2016-12-06 | NuTech Spine, Inc. | Preparations containing hepatocyte growth factor and hyaluronic acid and methods of making and using same |
| US9950035B2 (en) | 2013-03-15 | 2018-04-24 | Biomet Biologics, Llc | Methods and non-immunogenic compositions for treating inflammatory disorders |
| US9895418B2 (en) | 2013-03-15 | 2018-02-20 | Biomet Biologics, Llc | Treatment of peripheral vascular disease using protein solutions |
| US10143725B2 (en) | 2013-03-15 | 2018-12-04 | Biomet Biologics, Llc | Treatment of pain using protein solutions |
| US20140271589A1 (en) | 2013-03-15 | 2014-09-18 | Biomet Biologics, Llc | Treatment of collagen defects using protein solutions |
| US10208095B2 (en) | 2013-03-15 | 2019-02-19 | Biomet Manufacturing, Llc | Methods for making cytokine compositions from tissues using non-centrifugal methods |
| WO2014170411A1 (en) | 2013-04-16 | 2014-10-23 | Orbsen Therapeutics Limited | Medical use of syndecan-2 |
| AU2014262590B2 (en) | 2013-05-10 | 2018-05-10 | Children's Medical Center Corporation | Wound healing and tissue engineering |
| MX2016001247A (es) | 2013-07-30 | 2016-08-17 | Musculoskeletal Transplant Foundation | Matrices derivadas de tejido suave acelular y metodos para preparar las mismas. |
| JP6633522B2 (ja) | 2013-11-16 | 2020-01-22 | テルモ ビーシーティー、インコーポレーテッド | バイオリアクターにおける細胞増殖 |
| WO2015148704A1 (en) | 2014-03-25 | 2015-10-01 | Terumo Bct, Inc. | Passive replacement of media |
| EP3209768A1 (en) | 2014-07-25 | 2017-08-30 | Recellerate Inc. | Methods of treating exercise-induced pulmonary hemorrhage |
| US20160090569A1 (en) | 2014-09-26 | 2016-03-31 | Terumo Bct, Inc. | Scheduled Feed |
| US11083755B2 (en) | 2015-01-08 | 2021-08-10 | The Board Of Trustees Of The Leland Stanford Junior University | Factors and cells that provide for induction of bone, bone marrow, and cartilage |
| WO2016154019A1 (en) | 2015-03-20 | 2016-09-29 | Orbsen Therapeutics Limited | Modulators of syndecan-2 and uses thereof |
| CA3177726A1 (en) | 2015-05-21 | 2016-11-24 | Musculoskeletal Transplant Foundation | Modified demineralized cortical bone fibers |
| WO2017004592A1 (en) | 2015-07-02 | 2017-01-05 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
| US10912864B2 (en) | 2015-07-24 | 2021-02-09 | Musculoskeletal Transplant Foundation | Acellular soft tissue-derived matrices and methods for preparing same |
| US11052175B2 (en) | 2015-08-19 | 2021-07-06 | Musculoskeletal Transplant Foundation | Cartilage-derived implants and methods of making and using same |
| EP3922253A1 (en) | 2016-01-15 | 2021-12-15 | Orbsen Therapeutics Limited | Sdc-2 exosome compositions and methods of isolation and use |
| JP7034949B2 (ja) | 2016-05-25 | 2022-03-14 | テルモ ビーシーティー、インコーポレーテッド | 細胞の増殖 |
| US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
| US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
| CN117247899A (zh) | 2017-03-31 | 2023-12-19 | 泰尔茂比司特公司 | 细胞扩增 |
| US11624046B2 (en) | 2017-03-31 | 2023-04-11 | Terumo Bct, Inc. | Cell expansion |
| US12234441B2 (en) | 2017-03-31 | 2025-02-25 | Terumo Bct, Inc. | Cell expansion |
| JP7174758B2 (ja) | 2017-07-14 | 2022-11-17 | オーブセン セラピューティクス リミテッド | Cd39間質幹細胞の単離方法と使用 |
| CN119033819A (zh) | 2017-11-22 | 2024-11-29 | 迈索布拉斯特国际有限公司 | 细胞组合物及治疗方法 |
| MX2020011297A (es) | 2018-04-25 | 2020-11-18 | Childrens Medical Center | Ligandos y substratos de abcb5. |
| JP2021526556A (ja) | 2018-05-30 | 2021-10-07 | ダイレクト バイオロジクス エルエルシー | 成長因子および細胞外小胞を凍結または粉状にした、間葉系幹細胞(msc)の調製物を含む助剤、ならびにその使用方法 |
| KR20210121039A (ko) * | 2018-12-26 | 2021-10-07 | 다이렉트 바이오로직스 엘엘씨 | 피부 및 모발 장애를 치료하기 위한 방법 및 조성물 |
| BR112021015549A2 (pt) | 2019-02-07 | 2021-10-26 | Direct Biologics Llc | Método para tratar osteoartrite com uma combinação de exossomos de células tronco mesenquimais, células tronco mesenquimais sinoviais e andaimes |
| SG11202108114YA (en) * | 2019-02-27 | 2021-08-30 | Tigenix S A U | Improved stem cell populations for allogeneic therapy |
| CA3146433A1 (en) | 2019-07-18 | 2021-01-21 | Direct Biologics Llc | Preparations comprising mesenchymal stem cells and cannabinoids and methods of their use |
| US12590310B2 (en) | 2020-04-22 | 2026-03-31 | Direct Biologics, Llc | Methods and compositions for treating inflammatory conditions associated with infectious disease |
| EP4146787A4 (en) | 2020-05-08 | 2024-06-26 | Gallant Pet, Inc. | Uterine-derived regenerative cell compositions and uses thereof |
| WO2022103798A1 (en) * | 2020-11-10 | 2022-05-19 | Medregen, Llc | Formulations, methods, kits, and dosage forms |
| GB2619893A (en) | 2021-03-23 | 2023-12-20 | Terumo Bct Inc | Cell capture and expansion |
| US12152699B2 (en) | 2022-02-28 | 2024-11-26 | Terumo Bct, Inc. | Multiple-tube pinch valve assembly |
| USD1099116S1 (en) | 2022-09-01 | 2025-10-21 | Terumo Bct, Inc. | Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device |
| US12502407B2 (en) | 2024-04-25 | 2025-12-23 | Direct Biologics, Llc | Treatment of fistula with bone marrow mesenchymal stem cell derived extracellular vesicles |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL68218A (en) * | 1983-03-23 | 1985-12-31 | Univ Ramot | Compositions for cartilage repair comprising embryonal chondrocytes |
| US5226914A (en) * | 1990-11-16 | 1993-07-13 | Caplan Arnold I | Method for treating connective tissue disorders |
| US5591625A (en) * | 1993-11-24 | 1997-01-07 | Case Western Reserve University | Transduced mesenchymal stem cells |
| US6482231B1 (en) * | 1995-11-20 | 2002-11-19 | Giovanni Abatangelo | Biological material for the repair of connective tissue defects comprising mesenchymal stem cells and hyaluronic acid derivative |
| ES2329953T3 (es) * | 1996-04-19 | 2009-12-02 | Osiris Therapeutics, Inc. | Regeneracion e incremento de hueso utilizando celulas madre mesenquimales. |
| AU2808397A (en) * | 1996-04-26 | 1997-11-19 | Case Western Reserve University | Skin regeneration using mesenchymal stem cells |
| CA2296704C (en) * | 1997-07-14 | 2010-10-19 | Osiris Therapeutics, Inc. | Cardiac muscle regeneration using mesenchymal stem cells |
| AU9231998A (en) * | 1997-09-20 | 1999-04-12 | Osiris Therapeutics, Inc. | Antigen presenting mesenchymal stem cells |
| AU749675B2 (en) * | 1998-03-13 | 2002-07-04 | Mesoblast International Sarl | Uses for human non-autologous mesenchymal stem cells |
| AU755888B2 (en) * | 1998-03-18 | 2003-01-02 | Mesoblast International Sarl | Mesenchymal stem cells for prevention and treatment of immune responses in transplantation |
| US6368636B1 (en) * | 1998-03-18 | 2002-04-09 | Osiris Therapeutics, Inc. | Mesenchymal stem cells for prevention and treatment of immune responses in transplantation |
| AU2999199A (en) * | 1998-04-03 | 1999-10-25 | Osiris Therapeutics, Inc. | Mesenchymal stem cells as immunosuppressants |
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| DE69922933T2 (de) | 2005-12-29 |
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| WO1999046366A1 (en) | 1999-09-16 |
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| AU2904299A (en) | 1999-09-27 |
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