JP4267916B2 - 多糖体ｋ５から誘導された高度のアンチトロンビン活性を有するグリコサミノグリカン及びその製法 - Google Patents
多糖体ｋ５から誘導された高度のアンチトロンビン活性を有するグリコサミノグリカン及びその製法 Download PDFInfo
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- JP4267916B2 JP4267916B2 JP2002552018A JP2002552018A JP4267916B2 JP 4267916 B2 JP4267916 B2 JP 4267916B2 JP 2002552018 A JP2002552018 A JP 2002552018A JP 2002552018 A JP2002552018 A JP 2002552018A JP 4267916 B2 JP4267916 B2 JP 4267916B2
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- 238000002835 absorbance Methods 0.000 description 1
- VYTBPJNGNGMRFH-UHFFFAOYSA-N acetic acid;azane Chemical compound N.N.CC(O)=O.CC(O)=O.CC(O)=O.CC(O)=O VYTBPJNGNGMRFH-UHFFFAOYSA-N 0.000 description 1
- AEMOLEFTQBMNLQ-WAXACMCWSA-N alpha-D-glucuronic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-WAXACMCWSA-N 0.000 description 1
- 150000001412 amines Chemical group 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
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- 235000011130 ammonium sulphate Nutrition 0.000 description 1
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- 239000000010 aprotic solvent Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- 125000000524 functional group Chemical group 0.000 description 1
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- 239000011521 glass Substances 0.000 description 1
- 230000002008 hemorrhagic effect Effects 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
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- 239000003456 ion exchange resin Substances 0.000 description 1
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- AEMOLEFTQBMNLQ-CLQWQSTFSA-N l-iduronic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@H](O)[C@@H](O)[C@@H]1O AEMOLEFTQBMNLQ-CLQWQSTFSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
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- 238000005259 measurement Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- 150000002772 monosaccharides Chemical class 0.000 description 1
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- MWUXSHHQAYIFBG-UHFFFAOYSA-N nitrogen oxide Inorganic materials O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
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- 239000001301 oxygen Substances 0.000 description 1
- JRKICGRDRMAZLK-UHFFFAOYSA-L peroxydisulfate Chemical compound [O-]S(=O)(=O)OOS([O-])(=O)=O JRKICGRDRMAZLK-UHFFFAOYSA-L 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000013017 sartobind Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
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- 238000003786 synthesis reaction Methods 0.000 description 1
- DZLFLBLQUQXARW-UHFFFAOYSA-N tetrabutylammonium Chemical class CCCC[N+](CCCC)(CCCC)CCCC DZLFLBLQUQXARW-UHFFFAOYSA-N 0.000 description 1
- CENHPXAQKISCGD-UHFFFAOYSA-N trioxathietane 4,4-dioxide Chemical group O=S1(=O)OOO1 CENHPXAQKISCGD-UHFFFAOYSA-N 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000006150 trypticase soy agar Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
- A61K31/727—Heparin; Heparan
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/737—Sulfated polysaccharides, e.g. chondroitin sulfate, dermatan sulfate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Materials Engineering (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- General Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Vascular Medicine (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Diabetes (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/738,879 US20030023079A1 (en) | 2000-03-30 | 2000-12-18 | Polysaccharides derived from K5 polysaccharide having high anticoagulant and antithrombotic activities and process for their preparation |
| US09/950,003 US20020062019A1 (en) | 2000-03-30 | 2001-09-12 | Glycosaminoglycans derived from K5 polysaccharide having high anticoagulant and antithrombotic activities and process for their preparation |
| PCT/IB2001/002492 WO2002050125A2 (en) | 2000-12-18 | 2001-12-17 | Glycosaminoglycans derived from k5 polysaccharide having high antithrombin activity and process for their preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004516345A JP2004516345A (ja) | 2004-06-03 |
| JP4267916B2 true JP4267916B2 (ja) | 2009-05-27 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002552018A Expired - Fee Related JP4267916B2 (ja) | 2000-12-18 | 2001-12-17 | 多糖体ｋ５から誘導された高度のアンチトロンビン活性を有するグリコサミノグリカン及びその製法 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US20020062019A1 (es) |
| EP (1) | EP1358215B1 (es) |
| JP (1) | JP4267916B2 (es) |
| CN (1) | CN1295251C (es) |
| AT (1) | ATE524496T1 (es) |
| AU (1) | AU2235802A (es) |
| CA (1) | CA2432150C (es) |
| ES (1) | ES2371232T3 (es) |
| WO (1) | WO2002050125A2 (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8227449B2 (en) | 2000-03-30 | 2012-07-24 | Glycores 2000 S.R.L. | Glycosaminoglycans derived from K5 polysaccharide having high anticoagulant and antithrombotic activities and process for their preparation |
| ITMI20032498A1 (it) * | 2003-12-17 | 2005-06-18 | Pasqua Anna Oreste | Polisaccaridi antitrombotici a basso peso molecolare e |
| ITMI20010397A1 (it) * | 2001-02-27 | 2002-08-27 | Giorgio Zoppetti | Derivati altamente n,o-solfatati del polisaccaride k5 e loro preparazione |
| US20120253029A1 (en) | 2002-06-18 | 2012-10-04 | Pasqua Anna Oreste | Process for the preparation of highly o-sulfated epimerized derivatives of k5 polysaccharide and intermediates therein |
| EP1513880B1 (en) * | 2002-06-18 | 2010-10-20 | Glycores 2000 S.r.l. | Epimerized derivatives of k5 polysaccharide with a very high degree of sulfation |
| US8513407B2 (en) * | 2002-06-18 | 2013-08-20 | Glycores 2000 S.R.L. | Process for the preparation of N-acyl-(epi)K5-amine-O-sulfate-derivatives and products thus obtained |
| US20040171819A1 (en) | 2002-10-10 | 2004-09-02 | Aventis Pharma S.A. | Mixtures of polysaccharides derived from heparin, their preparation and pharmaceutical compositions containing them |
| ITMI20031618A1 (it) * | 2003-08-06 | 2005-02-07 | Inalco Spa | Derivati polisaccaridici dotati di alta attivita' |
| US7655445B2 (en) * | 2003-11-12 | 2010-02-02 | Massachusetts Institute Of Technology | Methods for synthesis of sulfated saccharides |
| JP2005290383A (ja) * | 2004-04-05 | 2005-10-20 | Seikagaku Kogyo Co Ltd | ６−ｏ−硫酸化ｎ−アセチルヘパロサン及び造血幹細胞増殖助剤 |
| US8450297B2 (en) * | 2004-08-16 | 2013-05-28 | Massachusetts Institute Of Technology | Rapid two-step synthesis of anti-coagulants |
| WO2007058592A1 (en) * | 2005-11-21 | 2007-05-24 | Ge Healthcare Bio-Sciences Ab | A method of chromatography using semi-synthetic heparin ligands |
| CN103214591B (zh) * | 2013-04-12 | 2015-11-04 | 中国科学院昆明植物研究所 | 一种含末端2,5-脱水塔罗糖或其衍生物的低分子量糖胺聚糖衍生物 |
| ITLO20130006A1 (it) * | 2013-11-06 | 2015-05-07 | He E Biochimiche G Ronzoni S R | Derivati carbossilati di glucosamminoglicani e loro uso come farmaci |
| CN103698425B (zh) * | 2013-12-12 | 2015-08-26 | 中国海洋大学 | 一种化学降解法获得及检测生物来源糖胺聚糖二糖的方法 |
| EP3279220A4 (en) * | 2015-03-31 | 2018-12-19 | Seikagaku Corporation | Method for sulfating glycosaminoglycan |
| CN107727762A (zh) * | 2017-09-28 | 2018-02-23 | 山东大学 | 一种亚硝酸降解分析低分子肝素二糖结构的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1230785B (it) | 1989-07-14 | 1991-10-29 | Italfarmaco Spa | Processo per la preparazione di polisaccaridi eparino simili e prodotti ottenuti. |
| FR2669932B1 (fr) * | 1990-12-03 | 1994-07-01 | Sanofi Sa | Nouvel heparosane-n,o-sulfate, son procede de preparation et les compositions pharmaceutiques qui le contiennent. |
| GB2254083A (en) | 1991-03-28 | 1992-09-30 | Italfarmaco Spa | Anticoagulants from e.coli saccharide |
| WO1995030424A1 (en) * | 1994-05-06 | 1995-11-16 | Glycomed Incorporated | O-desulfated heparin derivatives, methods of making and uses thereof |
| IT1271057B (it) | 1994-11-04 | 1997-05-26 | Inalco Spa | Polisaccaridi aventi un elevato contenuto di acido iduronico |
| IT1282994B1 (it) * | 1996-05-10 | 1998-04-03 | Inalco Spa | Derivati del polisaccaride k5 aventi elevata attivita' anticoagulante |
| IT1290814B1 (it) * | 1997-03-24 | 1998-12-11 | Istituto Scient Di Chimica E B | Glicosaminoglicani aventi elevata attivita' antitrombotica |
| IT1318432B1 (it) * | 2000-03-30 | 2003-08-25 | Inalco Spa | Glicosaminoglicani derivati dal polisaccaride k5 aventi elevataattivita' anticoagulante ed antitrombotica e processo per la loro |
-
2001
- 2001-09-12 US US09/950,003 patent/US20020062019A1/en not_active Abandoned
- 2001-12-17 CN CNB018227104A patent/CN1295251C/zh not_active Expired - Fee Related
- 2001-12-17 CA CA2432150A patent/CA2432150C/en not_active Expired - Fee Related
- 2001-12-17 ES ES01271394T patent/ES2371232T3/es not_active Expired - Lifetime
- 2001-12-17 AU AU2235802A patent/AU2235802A/xx not_active Withdrawn
- 2001-12-17 JP JP2002552018A patent/JP4267916B2/ja not_active Expired - Fee Related
- 2001-12-17 EP EP01271394A patent/EP1358215B1/en not_active Expired - Lifetime
- 2001-12-17 AT AT01271394T patent/ATE524496T1/de not_active IP Right Cessation
- 2001-12-17 WO PCT/IB2001/002492 patent/WO2002050125A2/en active IP Right Grant
-
2005
- 2005-01-07 US US11/030,156 patent/US20050215518A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002050125A3 (en) | 2002-08-22 |
| EP1358215B1 (en) | 2011-09-14 |
| ATE524496T1 (de) | 2011-09-15 |
| ES2371232T3 (es) | 2011-12-28 |
| CA2432150A1 (en) | 2002-06-27 |
| US20050215518A1 (en) | 2005-09-29 |
| US20020062019A1 (en) | 2002-05-23 |
| CA2432150C (en) | 2010-04-13 |
| AU2235802A (en) | 2002-07-01 |
| CN1295251C (zh) | 2007-01-17 |
| WO2002050125A2 (en) | 2002-06-27 |
| EP1358215A2 (en) | 2003-11-05 |
| CN1531555A (zh) | 2004-09-22 |
| JP2004516345A (ja) | 2004-06-03 |
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