JP4258577B2 - ポリ−３−ヒドロキシアルカン酸の製造法 - Google Patents
ポリ−３−ヒドロキシアルカン酸の製造法 Download PDFInfo
- Publication number
- JP4258577B2 JP4258577B2 JP26879198A JP26879198A JP4258577B2 JP 4258577 B2 JP4258577 B2 JP 4258577B2 JP 26879198 A JP26879198 A JP 26879198A JP 26879198 A JP26879198 A JP 26879198A JP 4258577 B2 JP4258577 B2 JP 4258577B2
- Authority
- JP
- Japan
- Prior art keywords
- fab
- coli
- poly
- acid synthase
- microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000002253 acid Substances 0.000 title claims description 25
- 238000000034 method Methods 0.000 title claims description 11
- 241000588724 Escherichia coli Species 0.000 claims description 33
- 244000005700 microbiome Species 0.000 claims description 23
- 108090000623 proteins and genes Proteins 0.000 claims description 23
- 108010039731 Fatty Acid Synthases Proteins 0.000 claims description 20
- 238000004519 manufacturing process Methods 0.000 claims description 14
- 101150035981 fabH gene Proteins 0.000 claims description 12
- 101100012355 Bacillus anthracis fabH1 gene Proteins 0.000 claims description 11
- 101100012357 Bacillus subtilis (strain 168) fabHA gene Proteins 0.000 claims description 11
- 101150023061 acpP gene Proteins 0.000 claims description 9
- 101100000756 Bacillus subtilis (strain 168) acpA gene Proteins 0.000 claims description 8
- 101150051130 acpP1 gene Proteins 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052799 carbon Inorganic materials 0.000 claims description 7
- 230000009466 transformation Effects 0.000 claims 2
- 101001001272 Homo sapiens Prostatic acid phosphatase Proteins 0.000 claims 1
- 102100035703 Prostatic acid phosphatase Human genes 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 description 19
- 239000013604 expression vector Substances 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 6
- 230000014509 gene expression Effects 0.000 description 6
- 102100022089 Acyl-[acyl-carrier-protein] hydrolase Human genes 0.000 description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 102100027329 Malonyl-CoA-acyl carrier protein transacylase, mitochondrial Human genes 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 3
- 101000937642 Homo sapiens Malonyl-CoA-acyl carrier protein transacylase, mitochondrial Proteins 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 235000003869 genetically modified organism Nutrition 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000000379 polymerizing effect Effects 0.000 description 3
- WHBMMWSBFZVSSR-UHFFFAOYSA-N 3-hydroxybutyric acid Chemical compound CC(O)CC(O)=O WHBMMWSBFZVSSR-UHFFFAOYSA-N 0.000 description 2
- 241000607516 Aeromonas caviae Species 0.000 description 2
- 241000252867 Cupriavidus metallidurans Species 0.000 description 2
- 241001131785 Escherichia coli HB101 Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 101150026389 fabF gene Proteins 0.000 description 2
- 101150090981 fabG gene Proteins 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- WHBMMWSBFZVSSR-GSVOUGTGSA-N (R)-3-hydroxybutyric acid Chemical compound C[C@@H](O)CC(O)=O WHBMMWSBFZVSSR-GSVOUGTGSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- HPMGFDVTYHWBAG-UHFFFAOYSA-N 3-hydroxyhexanoic acid Chemical compound CCCC(O)CC(O)=O HPMGFDVTYHWBAG-UHFFFAOYSA-N 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N EtOH Substances CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 241000256251 Spodoptera frugiperda Species 0.000 description 1
- 101150070497 accC gene Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 101150024406 fabD gene Proteins 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- -1 glucose and fructose Chemical class 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 238000011419 induction treatment Methods 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 101150068528 mabA gene Proteins 0.000 description 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N methanol Substances OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26879198A JP4258577B2 (ja) | 1998-09-22 | 1998-09-22 | ポリ−３−ヒドロキシアルカン酸の製造法 |
| PCT/JP1999/005185 WO2000017340A1 (fr) | 1998-09-22 | 1999-09-22 | Procede de production d'acide poly-3-hydroxyalcanoique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26879198A JP4258577B2 (ja) | 1998-09-22 | 1998-09-22 | ポリ−３−ヒドロキシアルカン酸の製造法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2000135083A JP2000135083A (ja) | 2000-05-16 |
| JP4258577B2 true JP4258577B2 (ja) | 2009-04-30 |
Family
ID=17463331
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP26879198A Expired - Fee Related JP4258577B2 (ja) | 1998-09-22 | 1998-09-22 | ポリ−３−ヒドロキシアルカン酸の製造法 |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP4258577B2 (fr) |
| WO (1) | WO2000017340A1 (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006025375A1 (fr) | 2004-08-31 | 2006-03-09 | Riken | Biopolyester thermiquement stable |
| CN101137744A (zh) * | 2005-03-24 | 2008-03-05 | 株式会社钟化 | 蓄积超高分子量聚酯的微生物 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0644875B2 (ja) * | 1986-08-26 | 1994-06-15 | 日本化薬株式会社 | 組換え遺伝子産物の製造方法 |
| JPH03272680A (ja) * | 1990-03-20 | 1991-12-04 | Res Assoc Util Of Light Oil | 遺伝子組換え菌株の培養方法 |
| EP0870053A4 (fr) * | 1995-12-19 | 2002-09-11 | Univ Minnesota | Procede metabolique de fabrication de synthases monomeres de polyhydroxyalcanoates |
| JPH11276180A (ja) * | 1998-03-31 | 1999-10-12 | Rikagaku Kenkyusho | ポリエステル合成能を有する植物及びポリエステルの製造方法 |
-
1998
- 1998-09-22 JP JP26879198A patent/JP4258577B2/ja not_active Expired - Fee Related
-
1999
- 1999-09-22 WO PCT/JP1999/005185 patent/WO2000017340A1/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000017340A1 (fr) | 2000-03-30 |
| JP2000135083A (ja) | 2000-05-16 |
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