JP4162774B2 - タンパク質製造方法 - Google Patents

タンパク質製造方法 Download PDF

Info

Publication number
JP4162774B2
JP4162774B2 JP27410498A JP27410498A JP4162774B2 JP 4162774 B2 JP4162774 B2 JP 4162774B2 JP 27410498 A JP27410498 A JP 27410498A JP 27410498 A JP27410498 A JP 27410498A JP 4162774 B2 JP4162774 B2 JP 4162774B2
Authority
JP
Japan
Prior art keywords
culture
fed
glucose
carbon source
glycerol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP27410498A
Other languages
English (en)
Japanese (ja)
Other versions
JPH11169173A (ja
Inventor
カルステン・ヘルムス
ルアル・ロペス−ウリバッリ
アン・フランソワ・マイヤー
ハインリッヒ・ヴィンフリート・シュリーカー
アドルフス・ファン・ルーン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of JPH11169173A publication Critical patent/JPH11169173A/ja
Application granted granted Critical
Publication of JP4162774B2 publication Critical patent/JP4162774B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP27410498A 1997-10-01 1998-09-29 タンパク質製造方法 Expired - Lifetime JP4162774B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97117021 1997-10-01
EP97117021.2 1997-10-01

Publications (2)

Publication Number Publication Date
JPH11169173A JPH11169173A (ja) 1999-06-29
JP4162774B2 true JP4162774B2 (ja) 2008-10-08

Family

ID=8227420

Family Applications (1)

Application Number Title Priority Date Filing Date
JP27410498A Expired - Lifetime JP4162774B2 (ja) 1997-10-01 1998-09-29 タンパク質製造方法

Country Status (12)

Country Link
US (1) US6204012B1 (US06559137-20030506-C00039.png)
EP (2) EP0911416B1 (US06559137-20030506-C00039.png)
JP (1) JP4162774B2 (US06559137-20030506-C00039.png)
KR (1) KR100572426B1 (US06559137-20030506-C00039.png)
CN (1) CN1215175C (US06559137-20030506-C00039.png)
AU (1) AU748187B2 (US06559137-20030506-C00039.png)
BR (1) BR9803807B1 (US06559137-20030506-C00039.png)
CA (1) CA2249061C (US06559137-20030506-C00039.png)
DE (1) DE69834533T2 (US06559137-20030506-C00039.png)
DK (1) DK0911416T3 (US06559137-20030506-C00039.png)
ES (1) ES2264181T3 (US06559137-20030506-C00039.png)
IN (1) IN188151B (US06559137-20030506-C00039.png)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
AU4056700A (en) * 1999-03-31 2000-10-16 Cornell Research Foundation Inc. Phosphatases with improved phytase activity
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
TWI332822B (en) 2001-10-31 2010-11-11 Phytex Llc Phytase-containing animal food and method
KR100429331B1 (ko) * 2002-01-29 2004-04-29 재단법인 목암생명공학연구소 재조합 효모를 이용한 에스에이치엘케이엔-1 단백질의대량생산방법
CA2492267C (en) * 2002-07-15 2013-09-10 Immunex Corporation Methods and media for controlling sialylation of proteins produced by mammalian cells
US7309505B2 (en) * 2002-09-13 2007-12-18 Cornell Research Foundation, Inc. Using mutations to improve Aspergillus phytases
CA2534352A1 (en) * 2003-08-08 2005-02-17 Arriva Pharmaceuticals, Inc. Methods of protein production in yeast
ES2375706T3 (es) * 2003-11-14 2012-03-05 Baxter International Inc. Composiciones de alfa 1-antitripsina y métodos de tratamiento que usan tales composiciones.
WO2006036678A2 (en) * 2004-09-22 2006-04-06 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
US20070105768A1 (en) * 2004-11-10 2007-05-10 Rajiv Nayar Dry recombinant human alpha 1-antitrypsin formulation
US9410175B2 (en) 2005-03-24 2016-08-09 Dsm Ip Assets B.V. Process for microbial production of a valuable compound
US7919297B2 (en) * 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
JP2009535038A (ja) * 2006-04-28 2009-10-01 シー５−６ テクノロジーズ インコーポレイテッド 耐熱性セルラーゼおよび使用方法
WO2008017066A2 (en) * 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
WO2008090211A1 (en) 2007-01-26 2008-07-31 Novozymes A/S Method for methanol independent induction from methanol inducible promoters in pichia
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
CN103060216B (zh) * 2008-05-06 2016-07-06 阿彻丹尼尔斯米德兰德公司 通过淀粉和木聚糖的降解酶的表达开发能进行淀粉和木聚糖的醇发酵的耐热酵母多形汉逊酵母菌株
GB201308828D0 (en) * 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
CN103159531A (zh) * 2013-04-17 2013-06-19 广西新方向化学工业有限公司 一种含多种植物醇和中微量元素螯合的液体肥料及其制备方法
JP2016525354A (ja) 2013-07-25 2016-08-25 ビーエーエスエフ エンザイムズ エルエルシー フィターゼ
CN114365792A (zh) 2014-11-11 2022-04-19 克莱拉食品公司 用于生成卵清蛋白的方法和组合物
CN104928271A (zh) * 2015-07-06 2015-09-23 唐亚军 一种木聚糖酶的制备方法
CN105200101B (zh) * 2015-10-19 2019-04-16 华中农业大学 一种毕赤酵母菌体重复发酵表达外源蛋白的方法
KR20220034848A (ko) 2019-07-11 2022-03-18 클라라 푸드즈 컴퍼니 단백질 조성물 및 이의 섭취 가능한 제품
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0173378B1 (en) 1984-07-27 1991-06-12 Unilever N.V. Process for preparing a polypeptide by culturing a transformed microorganism, a transformed microorganism suitable therefor and dna sequences suitable for preparing such microorganism
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
NL8601454A (nl) * 1986-06-05 1988-01-04 Unilever Nv Werkwijze voor het bereiden van een catalase-vrij oxidoreductase en van een catalase-vrije oxidoreductase bevattende gist, en gebruik daarvan.
ATE58169T1 (de) * 1986-11-24 1990-11-15 Unilever Nv Verfahren zur herstellung einer catalasefreien, oxidase enthaltenden hefe und deren verwendung.
EP0299108B1 (en) 1987-07-17 1994-05-18 Rhein Biotech Gesellschaft für biotechnologische Prozesse und Produkte mbH DNA-molecules coding for FMDH control regions and structured gene for a protein having FMDH-activity and their uses
JPH04501662A (ja) * 1988-09-26 1992-03-26 ザ・サルク・インスティチュート・バイオテクノロジー／インダストリアル・アソシエイツ・インコーポレーテッド 混合給送組換え酵母発酵
US5667986A (en) * 1989-10-18 1997-09-16 Delta Biotechnology Limited Yeast promoter for expressing heterologous polypeptides
CA2096650A1 (en) * 1991-09-18 1993-03-21 Keith E. Langley Hepatitis b vaccine formulation incorporating a bile acid salt
DK0684313T3 (da) 1994-04-25 2006-11-06 Dsm Ip Assets Bv Polypeptider med phytaseaktivitet
US6068995A (en) * 1994-10-25 2000-05-30 Yoshitomi Pharmaceutical Industries, Ltd. Method for producing protein
KR0161148B1 (ko) * 1995-10-11 1998-11-16 김은영 재조합 메탄올 자화 효모를 사용한 이종 단백질의 생산방법
CA2231948C (en) 1997-03-25 2010-05-18 F. Hoffmann-La Roche Ag Modified phytases

Also Published As

Publication number Publication date
DK0911416T3 (da) 2006-08-14
EP0911416A2 (en) 1999-04-28
JPH11169173A (ja) 1999-06-29
BR9803807A (pt) 2000-03-28
CA2249061C (en) 2009-02-24
EP0911416A3 (en) 2000-01-19
CN1215175C (zh) 2005-08-17
EP0911416B1 (en) 2006-05-17
IN188151B (US06559137-20030506-C00039.png) 2002-08-24
US6204012B1 (en) 2001-03-20
BR9803807B1 (pt) 2010-11-03
ES2264181T3 (es) 2006-12-16
KR19990036734A (ko) 1999-05-25
CA2249061A1 (en) 1999-04-01
EP1659173A1 (en) 2006-05-24
CN1218112A (zh) 1999-06-02
DE69834533T2 (de) 2006-10-12
KR100572426B1 (ko) 2006-06-21
AU748187B2 (en) 2002-05-30
AU8715798A (en) 1999-04-22
DE69834533D1 (de) 2006-06-22

Similar Documents

Publication Publication Date Title
JP4162774B2 (ja) タンパク質製造方法
WO2005052174A2 (en) Lactic acid producing yeast
KR20020048910A (ko) 유기 생성물의 합성방법 및 재료
CN109207373B (zh) 一株高产柠檬酸的微生物菌株及其发酵淀粉糖质生产柠檬酸的方法
JP2004515249A (ja) 菌類による異種たんぱく質の生産方法
Hoshino et al. Continuous ethanol production from raw starch using a reversibly soluble-autoprecipitating amylase and flocculating yeast cells
EP2062967A1 (en) Genetically engineered aspergillus
KR100405944B1 (ko) 단백질 제조방법
CN111849790B (zh) 重组顶头孢霉工程菌及其构建方法和应用
Lee et al. Mass production of thermostable D‐hydantoinase by batch culture of recombinant Escherichia coli with a constitutive expression system
CN109929853B (zh) 嗜热菌来源的热激蛋白基因的应用
CN116157530A (zh) 使用温度变化的芽孢杆菌工业发酵工艺
CN116234922A (zh) 使用进料速率变化的芽孢杆菌工业发酵工艺
CN113493785A (zh) 一种适用于谷氨酸棒杆菌的高强度启动子及应用
KR102613937B1 (ko) 갈락토스 이용에 관여하는 모든 유전자가 결손된 효모 균주 및 이를 이용한 재조합 단백질 생산방법
CN114717250B (zh) 一种基于辅因子代谢工程策略改造蛹虫草菌提高虫草素产量的方法及应用
Cha et al. Enhancement of production of cloned glucoamylase under conditions of low aeration from recombinant yeast using a SUC2 promoter
JP5006325B2 (ja) 酸素によって調節される微生物
JP3060019B2 (ja) マルトテトラオース生成酵素遺伝子を含有するプラスミド及び該プラスミドを有する微生物並びに該微生物を用いるマルトテトラオース生成酵素の製造法
WO2006040955A1 (ja) 凝集性アルコール発酵酵母の生産方法及び凝集性アルコール発酵酵母
Grøn et al. Production of proteinase A by Saccharomyces cerevesiae in a cell-recycling fermentation system: experiments and computer simulations
WO2024003943A1 (en) A novel proteins/peptides production platform in yeast
CN117210459A (zh) 环境诱导型启动子、含有该启动子的重组载体、重组菌及其应用
CN118126922A (zh) 一种l-苏氨酸生产菌株及其构建方法与应用
KR100472082B1 (ko) 재조합 단백질의 생산성 증가를 위한 재조합 효모의 배양방법

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20040427

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20040427

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20040922

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20061130

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20061204

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20070515

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20070813

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20070813

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20071211

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20080304

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20080501

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20080715

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20080723

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110801

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110801

Year of fee payment: 3

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120801

Year of fee payment: 4

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130801

Year of fee payment: 5

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term