JP4106626B2 - 蛍光画像計測方法および装置 - Google Patents

蛍光画像計測方法および装置 Download PDF

Info

Publication number
JP4106626B2
JP4106626B2 JP2003566530A JP2003566530A JP4106626B2 JP 4106626 B2 JP4106626 B2 JP 4106626B2 JP 2003566530 A JP2003566530 A JP 2003566530A JP 2003566530 A JP2003566530 A JP 2003566530A JP 4106626 B2 JP4106626 B2 JP 4106626B2
Authority
JP
Japan
Prior art keywords
light
autofocus
specimen
fluorescence
fluorescence image
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2003566530A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2003067230A1 (ja
Inventor
直広 野田
睦久 平岡
一仁 高橋
哲仁 成國
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fuji Electric Co Ltd
Original Assignee
Fuji Electric Systems Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuji Electric Systems Co Ltd filed Critical Fuji Electric Systems Co Ltd
Publication of JPWO2003067230A1 publication Critical patent/JPWO2003067230A1/ja
Application granted granted Critical
Publication of JP4106626B2 publication Critical patent/JP4106626B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/241—Devices for focusing
    • G02B21/244—Devices for focusing using image analysis techniques

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP2003566530A 2002-02-07 2003-02-06 蛍光画像計測方法および装置 Expired - Lifetime JP4106626B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2002030648 2002-02-07
JP2002030648 2002-02-07
PCT/JP2003/001224 WO2003067230A1 (fr) 2002-02-07 2003-02-06 Procede et dispositif de mesure d'une image fluorescente

Publications (2)

Publication Number Publication Date
JPWO2003067230A1 JPWO2003067230A1 (ja) 2005-06-02
JP4106626B2 true JP4106626B2 (ja) 2008-06-25

Family

ID=27677908

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2003566530A Expired - Lifetime JP4106626B2 (ja) 2002-02-07 2003-02-06 蛍光画像計測方法および装置

Country Status (3)

Country Link
JP (1) JP4106626B2 (fr)
AU (1) AU2003207248A1 (fr)
WO (1) WO2003067230A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9000399B2 (en) 2011-05-03 2015-04-07 Samsung Electronics Co., Ltd. Fluorescence detecting optical system and multi-channel fluorescence detection apparatus including the same

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7295316B2 (en) * 2004-01-14 2007-11-13 Applera Corporation Fluorescent detector with automatic changing filters
JP2005284136A (ja) * 2004-03-30 2005-10-13 Olympus Corp 観察装置および観察装置の焦点合わせ方法
JP4631324B2 (ja) * 2004-06-28 2011-02-16 株式会社ニコン 蛍光顕微鏡
JP4955244B2 (ja) * 2005-09-27 2012-06-20 横河電機株式会社 バイオチップ読み取り装置およびバイオチップ読み取り方法
JP2007108223A (ja) * 2005-10-11 2007-04-26 Olympus Corp 顕微鏡システム
CN101438147B (zh) * 2006-05-31 2011-09-28 奥林巴斯株式会社 生物试样摄像方法及生物试样摄像装置
JP5296686B2 (ja) * 2006-08-14 2013-09-25 ウエストオーバー サイエンティフィック，インコーポレイテッド 半導体蛍光アセンブリ及び顕微鏡
JP5499732B2 (ja) * 2009-06-23 2014-05-21 ソニー株式会社 生体サンプル像取得装置、生体サンプル像取得方法及び生体サンプル像取得プログラム
JP2011133366A (ja) * 2009-12-24 2011-07-07 Ihi Corp 微生物検出方法、フィルタ及び蛍光印配置板
JP5201185B2 (ja) * 2010-09-14 2013-06-05 オムロン株式会社 観察光学系およびレーザ加工装置
EP3466335A1 (fr) * 2011-12-21 2019-04-10 Catherine M. Shachaf Méthode pour l'imagerie de fluorecence avec autofocus
WO2016157458A1 (fr) * 2015-03-31 2016-10-06 株式会社ニコン Appareil de mesure, système de mesure, procédé de traitement de chaîne de signal, et programme
WO2021138633A1 (fr) * 2019-12-31 2021-07-08 Illumina, Inc. Fonctionnalité de mise au point automatique dans une analyse d'échantillon optique
KR20240022443A (ko) * 2021-06-18 2024-02-20 하마마츠 포토닉스 가부시키가이샤 측정 장치

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0344608A (ja) * 1989-07-12 1991-02-26 Brother Ind Ltd 自動焦点合わせ方法
GB9125603D0 (en) * 1991-12-02 1992-01-29 Gec-Marconi Limited Optical analysis system and positioning apparatus therefor
JPH09243921A (ja) * 1996-03-12 1997-09-19 Nikon Corp レーザ走査型蛍光顕微鏡
JP4166297B2 (ja) * 1997-05-14 2008-10-15 オリンパス株式会社 顕微鏡用自動焦点検出装置
JP4608043B2 (ja) * 1999-09-24 2011-01-05 オリンパス株式会社 顕微鏡用焦点検出装置
JP2001154104A (ja) * 1999-11-25 2001-06-08 Olympus Optical Co Ltd 顕微鏡装置
JP3736278B2 (ja) * 2000-04-12 2006-01-18 松下電器産業株式会社 生化学物質の観察方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9000399B2 (en) 2011-05-03 2015-04-07 Samsung Electronics Co., Ltd. Fluorescence detecting optical system and multi-channel fluorescence detection apparatus including the same

Also Published As

Publication number Publication date
AU2003207248A1 (en) 2003-09-02
JPWO2003067230A1 (ja) 2005-06-02
WO2003067230A1 (fr) 2003-08-14

Similar Documents

Publication Publication Date Title
JP4106626B2 (ja) 蛍光画像計測方法および装置
JP4288323B1 (ja) 顕微鏡装置及びそれを用いた蛍光観察方法
EP3446170B1 (fr) Système d'imagerie doté d'un détecteur d'image auxiliaire pour l'emplacement d'un échantillon
US20070196815A1 (en) Positive Selection Procedure for Optically Directed Selection of Cells
WO2007037439A1 (fr) Procédé de décision de position de point focal, dispositif de decision de position de point focal, dispositif de détection de lumière faible, et procédé de detection de lumiere faible
CN101821608A (zh) 用于对样本成像的方法和系统
JP4779518B2 (ja) 蛍光読取装置および微生物計数装置
US11041756B2 (en) Method and apparatus of filtering light using a spectrometer enhanced with additional spectral filters with optical analysis of fluorescence and scattered light from particles suspended in a liquid medium using confocal and non confocal illumination and imaging
JP2007003323A (ja) 撮影システム
JP4474655B2 (ja) 顕微鏡装置
CN108780216B (zh) 利用散射以降低源自发荧光并改善均匀性的成像系统和方法
US11199500B2 (en) Method and microscopy system for recording a microscopic fluorescence image of a sample region containing a biological sample
CN111220590A (zh) 病原微生物药敏的快速检测仪器及检测方法
CA2454294A1 (fr) Dispositif et procede d'analyse de microbes
US20200379227A1 (en) Method For Analyzing Fluorescent Particles in an Immunoassay
JP2021113806A (ja) 生体試料を熱サイクルするための装置、それを含む監視機器、およびそのような装置を使用して生体試料を熱サイクルする方法
JP7260127B2 (ja) 細菌検出方法及び細菌検出装置
JP4792880B2 (ja) 微生物計数方法および微生物計数装置
CN116802463A (zh) 具有共焦成像的用于微孔板的通用多功能检测系统
CN209961688U (zh) 病原微生物药敏的快速检测仪器
WO2021070259A1 (fr) Dispositif d'analyse et procédé d'analyse
KR101188233B1 (ko) 바이오칩을 위한 진단장치
JP2007097582A (ja) 微生物計数装置
KR102441156B1 (ko) 다파장 광원을 이용한 다중 분석장치
JP3102954U (ja) ファイバーピペットを励起光源とし光量検出器を内蔵した蛍光観察装置

Legal Events

Date Code Title Description
A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A712

Effective date: 20050603

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20071023

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20071219

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20080306

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20080319

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110411

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Ref document number: 4106626

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120411

Year of fee payment: 4

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313111

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120411

Year of fee payment: 4

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120411

Year of fee payment: 4

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130411

Year of fee payment: 5

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20140411

Year of fee payment: 6

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250