JP4036892B2 - 大腸菌においてヌクレアーゼ遺伝子を直接クローン化する方法 - Google Patents

大腸菌においてヌクレアーゼ遺伝子を直接クローン化する方法 Download PDF

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JP4036892B2
JP4036892B2 JP53045495A JP53045495A JP4036892B2 JP 4036892 B2 JP4036892 B2 JP 4036892B2 JP 53045495 A JP53045495 A JP 53045495A JP 53045495 A JP53045495 A JP 53045495A JP 4036892 B2 JP4036892 B2 JP 4036892B2
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dna
coli
gene
nuclease
indicator
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Expired - Fee Related
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JP53045495A
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Japanese (ja)
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JPH10500853A (ja
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フオメンコフ，アレクセイ
デイラ，デボラ・ケイ
ローリ，エリザベス・エイ
キユー，シユアン−ヤン
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ニユー・イングランド・バイオレイブス・インコーポレイテツド
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Priority claimed from US08/247,990 external-priority patent/US5498535A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP53045495A 1994-05-24 1995-05-15 大腸菌においてヌクレアーゼ遺伝子を直接クローン化する方法 Expired - Fee Related JP4036892B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/247,990 1994-05-24
US08/247,990 US5498535A (en) 1994-05-24 1994-05-24 Method for direct cloning of nuclease genes in E. coli
US08/356,898 1994-12-15
US08/356,898 US5492823A (en) 1994-05-24 1994-12-15 Method for direct cloning and producing the BsoBI restriction endonuclease in E. coli
PCT/US1995/006381 WO1995032281A1 (en) 1994-05-24 1995-05-15 A METHOD FOR DIRECT CLONING OF NUCLEASE GENES IN $i(E. COLI)

Publications (2)

Publication Number Publication Date
JPH10500853A JPH10500853A (ja) 1998-01-27
JP4036892B2 true JP4036892B2 (ja) 2008-01-23

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ID=26939024

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JP53045495A Expired - Fee Related JP4036892B2 (ja) 1994-05-24 1995-05-15 大腸菌においてヌクレアーゼ遺伝子を直接クローン化する方法

Country Status (5)

Country Link
US (1) US5492823A (https=)
EP (2) EP1431388B1 (https=)
JP (1) JP4036892B2 (https=)
DE (2) DE69532725T2 (https=)
WO (1) WO1995032281A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5492823A (en) 1994-05-24 1996-02-20 New England Biolabs, Inc. Method for direct cloning and producing the BsoBI restriction endonuclease in E. coli
US5950263A (en) * 1996-11-12 1999-09-14 Sunrise Medical Hhg Inc. Length extender for child's wheelchair seating system
US5945326A (en) * 1997-03-20 1999-08-31 New England Biolabs, Inc. Method for cloning and producing the Spel restriction endonuclease
WO1999064632A1 (en) * 1998-06-12 1999-12-16 New England Biolabs, Inc. Restriction enzyme gene discovery method
US6048731A (en) * 1998-09-03 2000-04-11 New England Biolabs, Inc. Method for cloning and producing the SgrAI restriction endonuclease
US6048719A (en) * 1999-01-22 2000-04-11 New England Biolabs, Inc. Method for cloning and producing the DraIII restriction endonuclease
US6245545B1 (en) 1999-04-27 2001-06-12 New England Biolabs, Inc. Method for cloning and producing the SwaI restriction endonuclease
US6191267B1 (en) 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
US6846658B1 (en) 2000-10-12 2005-01-25 New England Biolabs, Inc. Method for cloning and producing the Msel restriction endonuclease
US6403354B1 (en) * 2001-01-19 2002-06-11 New England Biolabs, Inc. Method for cloning and expression of BstYI restriction endonuclease and BstYI methylase in E. coli and purification of BstYI and M.BstYI enzymes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200333A (en) * 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
DE3802040A1 (de) * 1988-01-25 1989-08-03 Max Planck Gesellschaft System zur positiven selektion rekombinanter dna-molekuele
US5100793A (en) * 1988-03-07 1992-03-31 New England Biolabs, Inc. Method for producing the asei restriction endonuclease and methylase
US5492823A (en) 1994-05-24 1996-02-20 New England Biolabs, Inc. Method for direct cloning and producing the BsoBI restriction endonuclease in E. coli

Also Published As

Publication number Publication date
JPH10500853A (ja) 1998-01-27
EP0871715A4 (https=) 1998-10-21
EP0871715A1 (en) 1998-10-21
EP1431388A3 (en) 2004-11-10
EP1431388A2 (en) 2004-06-23
EP1431388B1 (en) 2009-12-02
DE69536025D1 (de) 2010-01-14
EP0871715B1 (en) 2004-03-17
DE69532725T2 (de) 2005-01-27
WO1995032281A1 (en) 1995-11-30
US5492823A (en) 1996-02-20
DE69532725D1 (de) 2004-04-22

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