JP3916189B2 - イムノアッセイ用試薬及びイムノアッセイ - Google Patents
イムノアッセイ用試薬及びイムノアッセイ Download PDFInfo
- Publication number
- JP3916189B2 JP3916189B2 JP10686898A JP10686898A JP3916189B2 JP 3916189 B2 JP3916189 B2 JP 3916189B2 JP 10686898 A JP10686898 A JP 10686898A JP 10686898 A JP10686898 A JP 10686898A JP 3916189 B2 JP3916189 B2 JP 3916189B2
- Authority
- JP
- Japan
- Prior art keywords
- immunoassay
- reagent
- reaction
- measurement
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 26
- 238000003018 immunoassay Methods 0.000 title claims description 18
- 238000006243 chemical reaction Methods 0.000 claims description 25
- 238000005259 measurement Methods 0.000 claims description 20
- 239000002245 particle Substances 0.000 claims description 20
- 239000004094 surface-active agent Substances 0.000 claims description 15
- 239000002736 nonionic surfactant Substances 0.000 claims description 14
- 125000000600 disaccharide group Chemical group 0.000 claims description 12
- 239000000427 antigen Substances 0.000 claims description 8
- 102000036639 antigens Human genes 0.000 claims description 8
- 108091007433 antigens Proteins 0.000 claims description 8
- 239000003125 aqueous solvent Substances 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 2
- 125000003071 maltose group Chemical group 0.000 claims 1
- 239000004816 latex Substances 0.000 description 20
- 229920000126 latex Polymers 0.000 description 20
- 238000000034 method Methods 0.000 description 16
- 230000002776 aggregation Effects 0.000 description 15
- 238000004220 aggregation Methods 0.000 description 13
- 239000000126 substance Substances 0.000 description 12
- 230000035945 sensitivity Effects 0.000 description 10
- 230000004520 agglutination Effects 0.000 description 9
- 239000011535 reaction buffer Substances 0.000 description 9
- 239000000523 sample Substances 0.000 description 9
- 230000001235 sensitizing effect Effects 0.000 description 9
- 238000011088 calibration curve Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 241000589886 Treponema Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000004793 Polystyrene Substances 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 229920002223 polystyrene Polymers 0.000 description 5
- KGUHOFWIXKIURA-VQXBOQCVSA-N [(2r,3s,4s,5r,6r)-6-[(2s,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-3,4,5-trihydroxyoxan-2-yl]methyl dodecanoate Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](COC(=O)CCCCCCCCCCC)O[C@@H]1O[C@@]1(CO)[C@@H](O)[C@H](O)[C@@H](CO)O1 KGUHOFWIXKIURA-VQXBOQCVSA-N 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 4
- 229940032085 sucrose monolaurate Drugs 0.000 description 4
- 206010029719 Nonspecific reaction Diseases 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000005054 agglomeration Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- NLEBIOOXCVAHBD-QKMCSOCLSA-N dodecyl beta-D-maltoside Chemical compound O[C@@H]1[C@@H](O)[C@H](OCCCCCCCCCCCC)O[C@H](CO)[C@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 NLEBIOOXCVAHBD-QKMCSOCLSA-N 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 208000006379 syphilis Diseases 0.000 description 2
- 239000013076 target substance Substances 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 238000011546 CRP measurement Methods 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000000644 isotonic solution Substances 0.000 description 1
- 238000000504 luminescence detection Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10686898A JP3916189B2 (ja) | 1998-03-13 | 1998-03-13 | イムノアッセイ用試薬及びイムノアッセイ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10686898A JP3916189B2 (ja) | 1998-03-13 | 1998-03-13 | イムノアッセイ用試薬及びイムノアッセイ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPH11258239A JPH11258239A (ja) | 1999-09-24 |
| JPH11258239A5 JPH11258239A5 (enrdf_load_stackoverflow) | 2005-09-02 |
| JP3916189B2 true JP3916189B2 (ja) | 2007-05-16 |
Family
ID=14444532
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10686898A Expired - Lifetime JP3916189B2 (ja) | 1998-03-13 | 1998-03-13 | イムノアッセイ用試薬及びイムノアッセイ |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3916189B2 (enrdf_load_stackoverflow) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4547110B2 (ja) * | 2000-07-27 | 2010-09-22 | シスメックス株式会社 | 全血免疫測定方法 |
| JP4879067B2 (ja) * | 2007-03-30 | 2012-02-15 | シスメックス株式会社 | 免疫測定用検体前処理液、免疫測定用試薬キット及び免疫測定方法 |
| JP5351054B2 (ja) | 2008-05-09 | 2013-11-27 | アークレイ株式会社 | 不溶性担体粒子の製造方法、不溶性担体粒子、測定試薬、検体分析用具および免疫比濁法 |
| CN119001079A (zh) * | 2024-07-11 | 2024-11-22 | 浙江夸烨生物科技有限公司 | 一种补体C1q检测试剂盒及应用 |
-
1998
- 1998-03-13 JP JP10686898A patent/JP3916189B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH11258239A (ja) | 1999-09-24 |
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