JP3118020B2 - Cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic comprising a crude drug extract having an anti-inflammatory action, a superoxide scavenging action, and / or an antibacterial action.

[0002]

2. Description of the Related Art Piper Ritrofuratum, Euphorbia pilifera, Coleus scutellaoides, Cryptokarya aromatika, Curcuma heineana, Trigonella hoenamuglaecum are plant herbal medicines used in Indonesia for folk treatment of eyes. is there. However, their physiological activities, ie, anti-inflammatory action, superoxide scavenging action, antibacterial action, etc., were not clear. On the other hand, since inflammation due to ultraviolet rays or the like is considered as one of the causes of the rough skin, cosmetics that reduce the inflammation and improve the rough skin are desired. In addition, superoxide is said to be involved in the production of lipid peroxide and promotion of aging, and an effect of eliminating it on the skin is desired. Further, it is desired that the cosmetic has an appropriate antibacterial action for the purpose of preserving cosmetics, preventing skin infection, and deodorant.

[0003]

Accordingly, a solvent extract of plant herbs Piper Ritroflatam, Euphorbia pilifera, Coleus scutellaoides, Cryptocarya aromatica, Curcuma heineana, Trigonella hoenamuglaecum and cosmetics Examination of application of the present invention revealed that the present invention exhibited excellent anti-inflammatory action (foot edema test), superoxide scavenging action, antibacterial action, and skin conditioning action, and was capable of being formulated into a pharmaceutical preparation.

[0004]

DISCLOSURE OF THE INVENTION The present invention relates to a plant crude drug, Piper returofuraotau.
m L.), Euphorbia pilufera
lifera L.), Coleus scuteraides (Coleus sc)
utellaioides Benth.), Cryptocarya aromatica Kostem, Curcuma heineana (Curcumaheyneana val.et v.Zjip), Trigonella foenum-graecum
A cosmetic comprising one or more solvent extracts selected from the group consisting of: As these herbal medicines, those marketed in Southeast Asia such as Indonesia can be used.

[0005] As a method for extracting a solvent extract of a crude drug, for example, there is a method in which a dried crude drug is finely cut, and a solvent is added for extraction. Examples of the solvent include one or more mixed solvents selected from water-soluble solvents such as water, ethanol, n-propanol, t-butanol, propylene glycol, and 1,3-butylene glycol. The extraction may be performed at room temperature or may be heated. The solvent extract can be concentrated or concentrated to dryness, if necessary, to facilitate subsequent operations. If necessary, polysaccharides such as starch contained in the solvent extract can be precipitated and removed by refrigeration or the like.

[0006] The solvent extract of crude drug is 0.001 to 10% by weight, preferably 0.01% by weight, based on the total amount of the cosmetic of the present invention.
~ 5.0% by weight. At a concentration of 0.001% by weight or less, a sufficient effect cannot be obtained, and at a concentration of 10% by weight or more, no enhancement of the effect is observed, which is uneconomical. In the cosmetic of the present invention, within a range that does not impair the effect of the solvent extract of the crude drug,
Fats and oils, waxes, hydrocarbons, used in cosmetics, etc.
Raw materials such as fatty acids, alcohols, esters, and surfactants can be blended. The cosmetics of the present invention are creams, lotions, emulsions, basic cosmetics such as packs,
Makeup cosmetics such as foundations and lipsticks, hair cosmetics such as hair creams, hair lotions, shampoos and rinses, bath preparations, soaps and other dosage forms can be used.

[0007]

EXAMPLES Next, the present invention will be described in detail with reference to Examples, but the present invention is not limited thereto. still,
The parts of the amounts shown in the examples are parts by weight. Example 1 Seed of Piper litrofuratum, Euphorbia pi
Lulifera leaf, Coleus scutellaoides leaf, ku
Bark of Liptokarya aromatica, Curcuma heinea
Drying of Rhizome, Trigonella hoenamuglaecum seeds
After chopping the dried material and adding 2 L of water to each 100 g,
The extraction was performed twice at 100 ° C. for 2 hours. Extract obtained
And then freeze-dried to obtain 3 to 25 g of hot water extract.
Was.

Example 2 A dried seed of Piper litrofuratum is chopped,
After adding 1 L of ethanol to each 100 g, 60
Reflux extraction was performed twice at 2 ° C. for 2 hours. The obtained extract was filtered and concentrated to dryness to obtain about 8 g of an ethanol extract of Piper litrofuratum seeds. Furthermore, leaves of Euphorbia pilifera, leaves of Coleus scutellaoides, bark of Cryptocarya aromatica, rhizomes of Curcuma heineana, and seeds of Trigonella hoenamuglaecum were similarly extracted. The extract amount of each crude drug was 5 to 10 g per 100 g of dry raw material.

Example 3 Dried leaves of Coleus scutellaoides were chopped, and 100 g of the leaf was mixed with 1 L of 1,3-butylene glycol (1,3-BG) and water (1: 1), and the mixture was stirred at room temperature. For 2 days. After extraction, the extracts were filtered to obtain about 900 ml of 1,3-BG extract of Coleus scutellaoides leaves. Furthermore, seeds of Piper litrofuratum, Euphorbia pilifera leaves, bark of Cryptocarya aromatica, rhizomes of Curcuma heineana, and seeds of Trigonella hoenamuglaecum were similarly extracted (yield 800-950 m).
l).

Example 4 Lotion Formulation Formulation amount 1. Ethanol 5.0 parts 2. Methyl P-oxybenzoate 0.1 3. Polyoxyethylene (40) hydrogenated castor oil 0.1 4. Appropriate amount of fragrance 5. Purified water 10.0 6. Examples The hot water extract of Euphorbia pilifera obtained in Example 1 0.05 7. The hot water extract of Coleus scutellaoides obtained in Example 1 0.05 8.1,3-butylene glycol 8.0 9. Glycerin 2.0 10. Xanthan gum 0.02 11 Purified water 74.5 Production method: Components 1 to 5 and components 6 to 11 are each dissolved uniformly, mixed and filtered to obtain a product.

Comparative Example 1 In the conventional lotion of Example 4, Euphorbia obtained in Example 1 was used.
What replaced | exchanged the hot water extract of Pirrifella and Coleus scutellaoides with purified water was used as the conventional lotion.

Example 5 Emulsion Formulation Compounding amount 1. Stearic acid 2.0 parts 2. Stearyl alcohol 2.0 3. Liquid paraffin 8.0 4. Glycerin monostearate 2.3 5. Sorbitan monooleate 2.5 6. Polyoxyethylene (10) sorbitan monooleate 0.8 7. Glycerin 6.0 8. Hot water extract of Piper litrofuratum obtained in Example 1 0.01 9. Methyl P-oxybenzoate 0.2 10. Purified water 77.4 11. Appropriate amount of perfume Production method: Oil phase components 1 to 6 And aqueous phase components 7 to 10
After heating and dissolving to 70-75 ° C, the aqueous phase component 1-6
Add 7 to 10 to emulsify, add component 11 while cooling and mix
Then, the product was cooled to 30 ℃.

Comparative Example 2 A conventional emulsion was prepared by replacing the hot water extract of Piper litrofuratum obtained in Example 1 in Example 5 with purified water.

Example 6 Pack Formulation Compounding amount 1. Polyvinyl alcohol 12.0 parts 2. Ethyl alcohol 5.0 3. 1,3-butylene glycol 8.0 4. Methyl P-oxybenzoate 0.2 5. Polyoxyethylene (40) hydrogenated castor oil 0.5 6. Ethanol extract of Coleus scutellaoides obtained in Example 2 0.05 7. Ethanol extract of Cryptocarya aromatica obtained in Example 2 0.05 8. Citric acid 0.1 9. Sodium citrate 0.3 10. Fragrance Appropriate amount 11. Purified water 73.8 Production method: Dissolve each component uniformly to obtain a product.

Example 7 Foundation formula Formulation amount 1. 0.25 part of 1,3-BG extract of Curcuma heineana obtained in Example 3. 2. Trigonella hoena glaecum obtained in Example 3 3-BG extract 0.25 3. Stearic acid 2.4 4. Polyoxyethylene (20) sorbitan monostearate 1.0 5. Polyoxyethylene (20) cetyl ether 2.0 6. Cetyl alcohol 1.0 7. Liquid lanolin 2.0 8. Liquid paraffin 3.0 9.Isopropyl myristate 6.5 10.P-butyl oxybenzoate 0.1 11.Purified water 58.2 12.Sodium carboxymethyl cellulose 0.1 13.Bentonite 0.5 14.Propylene glycol 4.0 15.Triethanolamine 1.1 16.Methyl P-oxybenzoate 0.2 17. Titanium oxide 8.0 18. Talc 4.0 19. Color pigment 5.0 20. Perfume Appropriate amount Production method: Heat components 1, 2 and 16 to 70 ° C to swell component 12 well The component 11 in which the component 13 is dispersed is added thereto and dissolved, and then the components 14 and 15 are dissolved to form an aqueous phase. Ingredients 3 to 10 are heated and dissolved, and kept at 80 ° C to form an oil phase.
The components 17 to 19, which are well mixed and passed through a pulverizer, are added to the aqueous phase, and the mixture is stirred with a homomixer and kept at 75 ° C. The oil phase is added to the aqueous phase with stirring, cooled, and the component 20 is added at 45 ° C.

Example 8 Hair Cream Formulation Compounding amount 1. Stearic acid 2.0 parts 2. Stearyl alcohol 2.0 3. Liquid paraffin 8.0 4. Glycerin monostearate 2.3 5. Sorbitan monooleate 2.5 6. Polyoxyethylene (10) sorbitan mono Olate 0.8 7. 1,3-BG extract of Euphorbia pilifera obtained in Example 3 0.2 8. Glycerin 6.0 9. Methyl P-oxybenzoate 0.1 10. Purified water 76.1 11. Appropriate perfume Production method: oil phase Components 1 to 6 and aqueous phase components 7 to 10 are each dissolved at 70 to 75 ° C., and aqueous phase components 7 to 10 are added to oil phase components 1 to 6 to emulsify, and while cooling, component 11 is added and mixed. , 30 ℃
Cool down to a product.

Example 9 Formulation of bath agent Compounding amount 1. Sodium sulfate 43.0 parts 2. Sodium bicarbonate 50.0 3. 1,3-BG extract of Coleus scutellaoides obtained in Example 3 5.0 4. (1) Appropriate amount 5. Appropriate amount of fragrance Manufacturing method: Mix each component well to make a product.

[0018]

EFFECT OF THE INVENTION Solvent extraction of the seeds of Piper litrofuratum of the present invention, leaves of Euphorbia pilifera, leaves of Colius scutellaoides, bark of Cryptocarya aromatica, rhizomes of Curcuma heineana, seeds of Trigonella foenum glaecum Things have excellent anti-inflammatory action,
The cosmetic, which has a superoxide elimination action and an antibacterial action, and contains a solvent extract of these crude drugs, exhibited an excellent skin conditioning action. Next, experimental examples will be described in order to explain the effects of the present invention in detail.

Experimental Example 1 Anti-inflammatory test The anti-inflammatory effect of each crude drug extract was examined using the rat carrageenan paw edema method. That is, male W weighing around 150g
Using crude ister rats, each crude drug extract of Examples 1 and 2 was subcutaneously administered to the back of the neck (500 mg / Kg body weight as dry residue)
did. One hour later, 0.1 ml of 1.0% carrageenin was subcutaneously administered to the sole of the right hind limb, and three hours after carrageenin administration, the foot volume of the right hind limb was measured. Physiological saline was used as a control. Table 1 shows the measurement results.

As apparent from Table 1, Examples 1 and 2
Extracts of each crude drug showed good anti-inflammatory action. Also,
No abnormalities such as weight loss were observed. Further, when the extract obtained in Example 3 was tested in the same manner, it showed a strong anti-inflammatory action comparable to that of Examples 1 and 2.

[0021]

[Table 1]

Experimental Example 2 Superoxide Elimination Test The superoxide elimination activity was measured by the nitrite method of Oyanagi et al. That is, 0.1 mM xanthine and 6.3 × 10-2U / ml
Superoxide is generated by xanthine oxidase (XOD), and nitrous acid generated by the reaction of the superoxide with hydroxylamine is colored with a color former,
Superoxide was quantified by absorbance at 550 nm. The superoxide elimination rate (%) was determined as a decrease rate of absorbance when a test substance or SOD was added to the reaction system.
Table 2 shows the results. When the test substance has an inhibitory effect on XOD, it is measured as an apparent superoxide elimination activity. Therefore, the inhibitory activity on XOD was also measured. That is, uric acid generated by the reaction between xanthine and XOD was quantified by absorbance at 290 nm, and the amount of uric acid was compared between the case where the test substance was added and the case where the test substance was not added, and it was clear that the test substance had no inhibitory effect on XOD. became.

The results are clear from Table 2. Among the hot water extracts of the crude drugs of Example 1, the leaves of Euphorbia pilifera, the leaves of Coleus scutellaoides, and the bark of Cryptocarya aromatica showed excellent superoxide elimination. Has an action. When the extracts of Euphorbia pilifera leaves, Colius scutellaoides leaves, and the bark of Cryptocarya aromatica were tested in the same manner as in Examples 2 and 3, the same activity as in Example 1 was obtained. Indicated.

[0024]

[Table 2]

Experimental Example 3 Antibacterial activity test The minimum inhibitory concentration (MIC) was measured by an agar plate dilution method. Specifically, one platinum loop of each bacterial solution was smeared onto a Mueller-Hinton (MH) agar medium in which each crude drug extract was dissolved to a concentration of 12.5 to 400 μg / ml, and the culture was grown at 37 ° C. for 20 hours. Was determined. The bacterial solution was prepared using MH broth for facultative anaerobic bacteria at 37 ° C. for 20 hours, and diluted with a phosphate buffer to 106 / ml. Table 3 shows the results.

As a result, among the hot water extracts of the crude drugs of Example 1, antibacterial activity was detected in Euphorbia pilifera leaves, Colius scutellaoides leaves, and the bark of Cryptocarya aromatica, and in particular, the antibacterial effect of Pseudomonas aeruginosa It showed high activity against one kind of Staphylococcus aureus. In addition, when the extracts of Euphorbia pilifera leaves, Colius scutellaoides leaves, and the bark of Cryptocarya aromatica were tested in the same manner as in Examples 2 and 3,
The antibacterial activity was almost the same as that of Example 1.

[0027]

[Table 3]

EXPERIMENTAL EXAMPLE 4 Skin Conditioning Using the lotion of Example 4, the lotion of Example 5, the conventional lotion of Comparative Example 1, and the conventional emulsion of Comparative Example 2, 15 women suffering from rough skin (20 ~ 35 years old) was subjected to a one-month use test. After use, the effect of improving skin roughness was determined. The results are shown in Table 2. As a result, the cosmetic containing the solvent extract of the seeds of Piper litrofuratum, the leaves of Euphorbia pilifera and the leaves of Coleus scutellaoides exhibited an excellent skin conditioning effect.

[0029]

[Table 4]

The pack of Example 6, the foundation of Example 7, and the bath agent of Example 9 were similarly subjected to use tests, and showed excellent skin conditioning.

The hair cream of Example 8 was also subjected to a use test similarly for women suffering from rough hair, and showed an excellent improving effect.

As described above, the solvent extract of Piper litroflatam, Euphorbia pilifera, Coleus scutellaoides, Cryptocarya aromatica, Curcuma heineana, Trigonella foenamuglaecum which is the herbal medicine of the present invention is excellent. The cosmetic, which has an anti-inflammatory action, a superoxide scavenging action, and an antibacterial action, and further contains one or more solvent extracts thereof, has an excellent skin conditioning effect and hair An improvement effect on roughness was shown.

Claims (1)

(57) [Claims] 1. One or more solvent extracts selected from the botanical herbs Piper Ritroflatam, Euphorbia pilifera, Coleus scutellaoides, Cryptocarya aromatica, Curcuma heineana, Trigonella hoenamuglaecum. Cosmetics characterized by containing.