JPH10203991A - Preparation for external use for skin - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a preparation for external use for skin with no need of using any toxic or irritant germicide or antiseptic which may act as allergen inducing allergy and is possible to affect living body, esp. human body, capable of retaining its long-term antimicrobial activity at least comparable to the case with using such germicides or antiseptics. SOLUTION: This preparation for external use for skin contains 0.001-20wt.% in terms of antimicrobial active ingredient, of at least one kind of extract selected from among those derived from Tilia cordata Mill., Tilia ulmifolia Scop., Tilia parvifolia Ehrh., Tilia platyphyllos Scop., Tilia grandifolia Ehrh. and Tiliaeuropaea L. each belonging to the genus Tiliaceae. The extraction is carried out by the use of a polar solvent such as 1,3-butylene glycol, ethanol or an aqueous solution thereof.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for skin containing an extract of a plant of the genus Lindus as an antibacterial component.

[0002]

2. Description of the Related Art At present, in various fields, contamination or deterioration of products caused by microorganisms is prevented.
Various bactericides and preservatives have been used to ensure the storage stability of products. Of these, directly applied to the living body,
Or, in the field of products ingested by living organisms, especially in the fields of cosmetics, pharmaceuticals, and foods, the types and amount of fungicides and preservatives that can be used may be determined to ensure the safety of living organisms. Many.

Specifically, in the cosmetics field, there are raw materials for which the upper limit of the blending concentration is specified by the Ministry of Health and Welfare notification, benzoic acid is 0.2% by weight, benzoates are 1.0% by weight, and salicylic acid is 0.2% by weight, salicylates 1.0% by weight, sorbic acid and its salts 0.5% by weight, dehydroacetic acid and its salts 0.5% by weight, paraoxybenzoates 1.0% by weight And 0.1% by weight of hexachlorophene is exemplified. These are also display designation components, and it is necessary to indicate that they are blended.

In the field of pharmaceuticals, benzoic acid, sodium benzoate, benzalkonium chloride, benzethonium chloride, cresol, chlorobutanol, thymol, methyl paraoxybenzoate, propyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate, phenol and benzyl Alcohol and the like are described in the Japanese Pharmacopoeia as preservatives. However, even if the type and the amount of use are limited in this way, some of them give an unpleasant sensation during use and have skin irritation.

[0005] Therefore, attempts have been made to impart antibacterial activity without using such a bactericide or preservative, and for example, a method of blending alcohol, a method of blending phenoxyethanol, and a method of using an antibacterial polyol. Method,
Methods using antibacterial components and extracts derived from animals and plants are known.

[0006] However, in the method of blending alcohol, phenoxyethanol, antibacterial polyol, antibacterial extract and the like, sufficient antibacterial activity cannot be obtained, and at room temperature, rot or mold grows during use. For this reason, compositions manufactured using these methods can be stored in containers in which microorganisms are unlikely to be mixed during production or in disposable small containers, or can be refrigerated after opening or used for a short period of time for antimicrobial treatment. Responding to lack of power.

[0007]

Accordingly, in the present invention, there is provided a bactericide which is toxic or irritating or may be harmful to living organisms, particularly to humans, and may be an allergen causing allergy. Without using preservatives,
It is an object of the present invention to provide a skin external preparation capable of maintaining a long-term antibacterial activity equivalent to or higher than the case of using these disinfectants and preservatives.

[0008]

In order to solve the above-mentioned problems, a high-stability, low-allergic, irritant and low-toxic fungicide and fungicide system was examined. In addition, it has been found that external preparations for skin such as pharmaceuticals and cosmetics containing the same have antibacterial activity without causing irritation or discomfort to the human body, especially the skin, and have completed the present invention. Was.

[0009]

Tilia plants used in DETAILED DESCRIPTION OF THE INVENTION The present invention is a kind of Tiliaceae plants (Tiliaceae), American linden (Tilia americana L.), Tilia cordata (Tilia
cordata Mill., Tilia ulmifolia Scop., Tilia parvi
folia Ehrh.) European linden ( Tilia europaea )
L.), linden ( Tilia japonica (Miq.) Simonk.), Herring tree ( Tiliakiusiana Makino et Shiras.), Squid ( Tilia maximowicziana Shiras.), Bodaiju ( T
ilia miqueliana Maxim.), Natsubodaiju ( Tilia plat
yphyllos Scop., Tilia grandifolia Ehrh.) and the like.

[0010] The linden plants such as Bodaiju are widely used as folk medicine in Europe, and the essential oil obtained from the dried inflorescence is used as a sweat, an antispasmodic or a bathing agent. It has been used for burns and wound healing.

The extraction site for obtaining the extract of the genus Lindensis is not particularly limited, but the bark and its mucus, fruit,
Examples include seeds, flowers, branches and leaves, and among them, an extract of a flower of the genus Linus is most preferred from the viewpoint of antibacterial activity.

When an extract is obtained from these plants, the plant can be extracted in a raw or dried state. However, from the viewpoint of odor and storage stability, it is preferable to use the dried product as it is or after pulverizing it. preferable.

[0013] Among the above-mentioned plants of the genus Linus, from the viewpoint of antibacterial activity, it is said that T. cordata ( Tiliacordata Mill., Til .
ia ulmifolia Scop., Tilia parvifolia Ehrh.), Natsudaidai ( Tilia platyphyllos Scop., Tilia grandif)
olia Ehrh.), European linden ( Tilia europaea L.)
It is preferable to use one or more plant extracts selected from

Solvents for obtaining these plant extracts include water, ethanol, methanol, isopropanol,
Alcohols such as isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol, n-octyl alcohol, glycerin, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, propylene glycol, propylene glycol monomethyl ether, propylene Glycol monoethyl ether, triethylene glycol,
Polyhydric alcohols such as 1,3-butylene glycol and hexylene glycol or derivatives thereof, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, and methyl-n-propyl ketone; esters such as ethyl acetate and isopropyl acetate; ethyl ether One or a mixture of two or more polar solvents such as ethers such as isopropyl ether, n-butyl ether and the like can be preferably used.
There is no particular limitation. Alternatively, aliphatic hydrocarbons such as petroleum ether, n-hexane, n-pentane, n-butane, n-octane, cyclohexane, carbon tetrachloride, chloroform,
One or a mixture of two or more of nonpolar solvents such as dichloromethane, trichloroethylene, benzene, and toluene can also be suitably used.

The solvent from which the extract of the genus Lindenium is obtained is not particularly limited as long as it is the above solvent, but a polar solvent is preferable from the viewpoint of antibacterial action.
One or more mixed solvents of 1,3-butylene glycol and water are preferable, and among them, 50% by weight as an extraction solvent is preferred.
1,3-butylene glycol aqueous solution is most preferred.

Further, as an extraction method, a method of extracting by impregnation at room temperature, in a cooled or heated state, a method of extraction using a distillation method such as steam distillation, an extract by pressing from a raw plant of the genus Lindwood. And the like. Extraction is performed using these methods alone or in combination of two or more.

The ratio of the plant to the solvent at the time of extraction is not particularly limited.
It is preferably 0 times by weight, especially 5 to 100 times by weight in terms of extraction operation and efficiency. The extraction temperature is conveniently in the range from room temperature to the boiling point of the solvent under normal pressure, and the extraction time varies depending on the extraction temperature and the like, but is preferably in the range of 2 hours to 2 weeks.

[0018] The extract of the genus Lindus plant thus obtained can be used as it is,
In addition, it can be blended after deodorizing, purifying and the like are added within a range that does not lose the fungicidal and fungicidal action, and can also be used as a fraction using column chromatography or the like. Further, these extracts, deodorized, purified, and fractionated products can be dried by removing the solvent therefrom, and further provided in the form of a solution solubilized in a solvent such as alcohol or in the form of an emulsion. can do.

The extract of the plant of the genus Linus as obtained above is incorporated as an antibacterial active ingredient into an external preparation for skin such as pharmaceuticals, quasi-drugs and cosmetics.

The external preparation for skin can be in any form such as cream, ointment, lotion, emulsion, solid, powder and the like. In particular, as cosmetics, basic cosmetics such as lotion, milky lotion, serum, moisturizing cream, sun care products such as sun cream, sun lotion, sun oil, carmine lotion, foundation, eyeliner, mascara, eye color, Makeup cosmetics such as teak color and lipstick, facial cleansers, body shampoos, hair shampoos and other cleaning agents, rinses, treatments, hair creams, hair oils, hair cosmetics and other hair cosmetics, perfumes, deodorants and antiperspirants Can be used for applications. At this time, various components generally used in cosmetics such as avocado oil, palm oil, peanut oil, rice bran oil, jojoba oil, and the like, as long as the effects of the present invention are not impaired.
Oil components such as orange roughy oil, macadamia nut oil, squalane, evening primrose oil, sesame oil, sunflower oil, safflower oil, carola oil, carnauba wax, paraffin wax, lanolin, diisostearyl malate, isostearyl alcohol, and liquid paraffin Humectants such as glycerin, diglycerin, polyglycerin, sorbit, polyethylene glycol, 1,3-butylene glycol, collagen, hyaluronic acid, vitamin A such as vitamin A oil, retinol, retinol acetate, riboflavin, riboflavin butyrate, etc. vitamin B _2, vitamin B ₆ such as pyridoxine hydrochloride, L- ascorbic acid, magnesium L- ascorbyl phosphate, L- vitamin C such as sodium ascorbate, etc., calcium pantothenate, D- pantothenyl alcohol, Pas Pantothenic acids such as nonthenyl ethyl ether and acetyl pantothenyl ethyl ether;
Vitamin D such as ergocalciferol and cholecalciferol, nicotinic acid such as nicotinic acid, nicotinamide, and benzyl nicotinate; vitamin Es such as α-tocopherol and tocopherol acetate; vitamins such as vitamin P and biotin; Benzophenone derivatives such as 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, sodium 2-hydroxy-4-methoxybenzophenone-5-sulfonate, para-aminobenzoic acid, ethyl para-aminobenzoate, Derivatives such as para-aminobenzoic acid derivatives such as octyl para-dimethylaminobenzoate, methoxycinnamic acid derivatives such as glyceryl 2-methoxyhexanoate and mono-2-ethylhexanoate, and salicyls such as octyl salicylate and myristyl salicylate. Derivatives, urocanic acid, 4-te
UV absorbers such as rt-butyl-4'-methoxydibenzoylmethane, 2- (2'-hydroxy-5'-methylphenyl) benzotriazole, guar gum, locust bean gum, carrageenan, quince seed, pectin, mannan, etc. Plant natural polysaccharides, microbial natural polysaccharides such as xanthan gum, dextran, curdlan, hyaluronic acid, animal polymers such as gelatin, casein, albumin, collagen, methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose And other semi-synthetic polymers such as cellulose, soluble starch, carboxymethyl starch, and methyl starch; semi-synthetic polymers such as propylene glycol alginate and alginates; Synthetic polymers such as benzyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, sodium polyacrylate, and polyethylene oxide; water-soluble polymers such as bentonite, laponite, and colloidal alumina; and dibutylhydroxytoluene, butylhydroxyanisole, Antioxidants such as gallic acid ester, higher fatty acid soap, alkyl sulfate ester salt, polyoxyethylene alkyl ether sulfate salt, acyl methyl taurine salt, alkyl ether phosphate ester salt, acyl amino acid salt and other anionic surfactants, alkyl chloride Cationic surfactants such as trimethylammonium, dialkyldimethylammonium chloride, and benzalkonium chloride; betaine alkyldimethylaminoacetate; Down, 2
Amphoteric surfactants such as alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, polyoxyethylene alkyl ether, polyoxyethylene alkyl phenyl ether, polyoxyethylene polyoxypropylene glycol, polyoxyethylene polyoxypropylene alkyl Ether, polyoxyalkylene fatty acid glycerin ester, polyoxyalkylene sorbitan ester, sorbite oligomeric tetraester, sorbite oligomeric hexaester, polyethylene glycol ester, polyoxyalkylene glycerin fatty acid ester, polyoxyalkylene sorbitan fatty acid ester, sucrose fatty acid Nonionic surfactants such as esters, alkylolamides and fatty acid amides, sodium ethylenediaminetetraacetate, poly Sodium phosphate,
Sequestering agents such as citric acid, sodium metaphosphate, succinic acid, gluconic acid, etc., whitening agents such as placenta extract, soybean arbor extract, glutathione, kojic acid and derivatives thereof, hydroquinone such as hydroquinone glycosides and derivatives thereof Anti-inflammatory agents such as glycyrrhizic acid, glycyrrhetinic acid, allantoin, azulene, hydrocortisone, ε-aminocaproic acid, astringents such as zinc oxide, allantoin hydroxyaluminum, aluminum chloride, tannic acid, citric acid, lactic acid, menthol, camphor and the like Cooling agents, antihistamines such as diphenhydramine hydrochloride and chlorpheniramine maleate, estradiol,
Sebum depressants such as estrone and ethinyl estradiol, exfoliants and dissolving agents such as salicylic acid and resorcinol, α
-Hydroxy acids and the like can be blended.

When the extract of linden genus is incorporated as an antibacterial active ingredient into an external preparation for skin such as pharmaceuticals, quasi-drugs, and cosmetics, the amount of the extract, the scent when added,
Considering the color tone, it is desirable to set the concentration range of 0.001 to 20% by weight.

[0022]

[Action] [Preparation method of extract of linden plant] 30 g of dried flower of Fujudaiju is 3 times 50% by weight of 1,3-
After being immersed in butylene glycol for one week, the mixture was filtered to obtain a Fujudaiju extract.

A safety test and a test for antibacterial activity of primary irritation, cumulative skin irritation and acute toxicity were carried out using the Fujudaiju extract. The primary skin irritation, cumulative skin irritation test, and antibacterial activity test were performed using a 10% by weight aqueous solution.

"Primary skin irritation test" A 10% by weight aqueous solution of a Fujudaiju extract was affixed to the skin of rabbits (3 per group) whose back was depilated. Judgment is 24,4 after pasting
After 8,72 hours, evaluation was made according to the criteria shown in Table 1, and the average value of the skin irritation index relating to the occurrence of erythema and edema was obtained. Table 2 shows the evaluation results.

[0025]

[Table 1]

[0026]

[Table 2]

As shown in the results of the primary skin irritation test shown in Table 2, erythema and edema were not observed in most animals, and only slight transient erythema was observed after only 48 hours. Was. This indicated that the Fujudaiju extract according to the present invention did not have primary skin irritation.

"Skin Cumulative Irritation Test" One Hartley-type guinea pig (female, 5 per group) with hair removed from the flank
Once a day, 5 times a week, 0.5 ml / 8 cm ² of a 10% by weight aqueous solution of a Fujudaiju extract was applied. Application was performed over 4 weeks and hair removal was performed on the last application day of each week. Judgment was evaluated on the day following the last day of each week according to the criteria shown in Table 1, and the average value of the skin irritation index relating to the occurrence of erythema and edema was determined. Table 3 shows the evaluation results.

[0029]

[Table 3]

As shown in the results of the cumulative skin irritation test shown in Table 3, erythema and edema were not observed in most animals. Some erythema was observed at some 3 weeks, but it was temporary erythema, and no erythema was observed on the next determination day. The occurrence of slight erythema was considered to be due to physical stimulation by hair clippers during hair removal. This indicated that the Fujudaiju extract of the present invention did not have cumulative skin irritation.

[Acute Toxicity Test] 2 g / kg of Fujudaiju extract was orally administered to mice (5 mice per group) which had been fasted for 4 hours before the test, and the occurrence and degree of toxicity symptoms were observed over time. As a result, no abnormalities were observed in all the mice for 14 days, and no abnormal findings were observed in the dissection results.

"Antibacterial activity test" 1
Using a 0% by weight aqueous solution, an antibacterial activity test against various bacteria and fungi was conducted. Escherichia as bacteria
coli ), Staphylococcus aureus ,
Pseudomonas aeruginosa , fungi as Candida albicans , black mold ( Aspergillus niger )
Was used to inoculate 10 ⁶ bacteria and 10 ⁵ fungi per gram of the sample, culture at 37 ° C. and 25 ° C., respectively, and measure the viable cell count after 2 weeks. In addition, the antibacterial activity was judged to be acceptable when the bacteria died after 2 weeks, and when the viable count of the fungi became 1/1000 or less, the bacteria passed. The results of the antibacterial test are shown in Table 4 as "O" for those that passed and "X" for those that failed.

[0033]

[Table 4]

As shown in Table 4, the Fujudaiju extract exhibited excellent antibacterial activity against all the bacteria and fungi tested.

[0035]

EXAMPLES Further, the features of the present invention will be described in detail with reference to examples.

Example 1 Lotion (1) Ethanol 2.0 (% by weight) (2) 1,3-butylene glycol 6.0 (3) Glycerin 2.0 (4) Polyoxyethylene hydrogenated castor oil 0 .6 (5) Natsubodaiju flower, 50% by weight aqueous 1,3-butylene glycol extract 1.0 (6) Fragrance 0.06 (7) Purified water 88.34 Production method: each of (1) to (6) Add the components sequentially to (7) and mix uniformly.

Example 2 Emulsion (1) Squalane 4.0 (% by weight) (2) Glyceryl tri-2-ethylhexanoate 2.0 (3) Cetyl 2-ethylhexanoate 3.0 (4) Cetanol 0.6 (5) Stearyl alcohol 0.4 (6) Sorbitan monostearate 1.2 (7) 1,3-butylene glycol 6.0 (8) Glycerin 4.0 (9) Polyoxyethylene (20EO) Sorbitan 0.8 monostearic acid ester (10) Purified water 72.9 (11) Aesculus sylvestris flower, 50% by weight 1,3-butylene glycol aqueous solution extract 5.0 (12) Fragrance 0.1 Production method: First, ( The oil phases 1) to (6) are mixed, heated and melted, and kept at 75 ° C. On the other hand, the aqueous phases (7) to (10) are mixed and dissolved by heating to 75 ° C., and the oil phase is added thereto with stirring to emulsify. After cooling, add (11) and (12) at 40 ° C and mix.

Example 3 Cream (1) Stearyl alcohol 6.0 (% by weight) (2) Stearic acid 2.0 (3) Hydrogenated lanolin 4.0 (4) Squalane 9.0 (5) Octyldodeca Knol 10.0 (6) Polyoxyethylene (25EO) cetyl alcohol ether 3.0 (7) Glyceryl monostearate 2.0 (8) 1,3-butylene glycol 6.0 (9) Purified water 56.4 (10) Fujudaiju leaf, 50% by weight aqueous ethanol extract 1.5 (11) Fragrance 0.1 Production method: Mix the oil phase components (1) to (7) and heat to 75 ° C. On the other hand, the aqueous phase components (8) and (9) were mixed and heated to 7
The temperature was adjusted to 5 ° C., and the oil phase was added to the mixture and emulsified.
At 0 ° C., add (10) and (11).

[Example 4] Oil-based cleansing cream (1) Celesin 8.0 (% by weight) (2) Microcrystalline wax 5.0 (3) White petrolatum 35.0 (4) Squalane 49.5 (5) Nuts Bodaiju flower, 1,3-butylene glycol extract 0.5 (6) Magnesium stearate 2.0 Manufacturing method: Components (1) to (6) are mixed and heated to gel,
Cooling.

Example 5 Emulsion Foundation (1) Stearic acid 2.4 (% by weight) (2) Propylene glycol monostearate 2.0 (3) Cetostearyl alcohol 0.2 (4) Liquid lanolin 0 (5) Liquid paraffin 3.0 (6) Isopropyl myristate 8.5 (7) Sodium carboxymethylcellulose 0.2 (8) Bentonite 0.5 (9) Propylene glycol 4.0 (10) Triethanolamine 1. 1 (11) Purified water 55.5 (12) Solanaceae flower ・ 50% by weight aqueous ethanol extract 2.5 (13) Fragrance 0.1 (14) Titanium oxide 8.0 (15) Talc 4.0 (16 ) Bengala 3.0 (17) Yellow iron oxide 2.5 (18) Black iron oxide 0.5 Production method: After mixing the pigments of (14) to (18), pulverize with a pulverizer. (11) is heated to 70 ° C., and (8) is added to swell well. To this is added (7) previously dispersed in (9), and then (10) is added and dissolved. The oil phases (1) to (6) are mixed, heated and melted to 80 ° C. The pigment is added to the aqueous phase with stirring, and the mixture is heated to 75 ° C. through a colloid mill. The oil phase is added with stirring to emulsify. After cooling, (12) and (13) are added at 40 ° C.

[Example 6] Aqueous suspension type mascara (1) 50% by weight of vinyl acetate emulsion 30.0 (% by weight) (2) Sodium carboxymethyl cellulose 1.0 (3) 1,3-butylene glycol 3.0 (4) Linden bark ・ 50 wt% 1,3-butylene glycol aqueous solution extract 5.0 (5) Titanium oxide 8.0 (6) Carbon black 1.6 (7) Bengala 0.4 (8) Purified water 51 0.0 Production method; (2) to (4) were added to (8) and dissolved,
(5) to (7) are added and dispersed through a colloid mill. Add (1) to this and disperse uniformly.

Example 7 Shampoo (1) Sodium alkyl ether sulfate 18.0 (% by weight) (2) Coconut oil fatty acid diethanolamide 2.0 (3) Bodaiju fruit / 50% by weight aqueous ethanol extract 5.0 (4) 1% by weight aqueous solution of Yellow No. 4 0.1 (5) Fragrance 0.1 (6) Purified water 74.8 Production method; (1) to (5) are sequentially added to (6) and uniformly mixed, Allow to dissolve.

Example 8 Hair rinse (1) Cetanol 3.0 (% by weight) (2) Stearyltrimethylammonium chloride 0.7 (3) Glycerin 3.0 (4) Green 3 1% by weight aqueous solution 0.2 (5) Fujudaijyu flower, 50% by weight aqueous ethanol extract 5.0 (6) Fragrance 0.1 (7) Purified water 88.0 Production method; (3) and (4) were added to (7), and 70 ° C To an aqueous phase. On the other hand, (1) and (2) are mixed and dissolved, and heated to 70 ° C. to form an oil phase. The oil phase is gradually added to the aqueous phase with stirring to pre-emulsify. After emulsifying with a homomixer, cool,
Then, (5) and (6) are added.

Example 9 Hair Cream (1) Polyoxyethylene (10EO) behenyl ether 5.0 (% by weight) (2) Hexyldecanol 5.0 (3) Liquid paraffin 35.0 (4) Purified lanolin 2 0.0 (5) Cetanol 2.0 (6) Glycerin 5.0 (7) Natsudadaiju leaf ・ 50% by weight of 1,3-butylene glycol aqueous solution extract 0.5 (8) Purified water 45.4 (9) Fragrance 0.1 Production method: The oil phase components (1) to (5) are mixed and heated to 75 ° C. On the other hand, the aqueous phase components (6) to (8)
The temperature was adjusted to 5 ° C., and the oil phase was added to the mixture and emulsified.
At 0 ° C. add (9).

Example 10 Hair Treatment (1) Polyoxyethylene (30EO) behenyl ether 4.0 (% by weight) (2) Self-emulsifying glyceryl monostearate 6.0 (3) Isopropyl myristate 0 (4) hexyldecanol 5.0 (5) squalane 3.0 (6) purified lanolin 3.0 (7) stearic acid 5.0 (8) lauryl dimethylaminoacetate betaine 5.0 (9) glycerin 5.0 ( 10) Purified water 54.8 (11) Fragrance 0.2 (12) Aesculus sylvestris flower ・ 50% by weight aqueous 1,3-butylene glycol extract 4.0 Manufacturing method: oil phase components of (1) to (7) Mix and heat to 80 ° C. On the other hand, the aqueous phase components (8) to (10) were mixed and heated to 8
The temperature was adjusted to 5 ° C., and the oil phase was added to the mixture and emulsified.
Add (11) and (12) at 0 ° C.

Example 11 Face Wash (1) Liquid paraffin 25.0 (% by weight) (2) Cetanol 2.0 (3) Lanolin 2.0 (4) Glyceryl monostearate 5.0 (5) Poly Oxyethylene stearyl ether 5.0 (6) Polyoxyethylene (20EO) sorbitan monostearate 1.0 (7) Glycerin 6.0 (8) Purified water 51.4 (9) Fragrance 0.1 (10) Fujudaiju Flower ・ 50% by weight 1,3-butylene glycol aqueous solution extract 2.5 Production method: After mixing and dissolving the oil phase of (1) to (6) and the aqueous phase of (7) and (8) at 65 ° C., respectively. Then, the aqueous phase is added to the oil phase and emulsified. After cooling, (9) and (10) are added and mixed at 40 ° C.

Example 12 Body Shampoo (1) Sodium lauryl ether sulfate 20.0 (% by weight) (2) Sodium myristyl ether sulfate 10.0 (3) Capric acid diethanolamide 3.0 (4) Polyoxypropylene (10PO) polyoxyethylene (20EO) cetyl ether 3.5 (5) sodium chloride 2.5 (6) vegetative flower / water extract 2.0 (7) fragrance 0.3 (8) purified water 58.7 Production method: The components (1) to (7) are sequentially added to (8) and mixed uniformly.

Example 13 Toothpaste (1) Calcium pyrophosphate 42.0 (% by weight) (2) Sodium lauryl sulfate 1.2 (3) Glycerin 10.0 (4) Carrageenan 1.1 (5) Sucrose Fatty acid ester 2.0 (6) Natsubodaiji ・ 75% by weight 1,3-butylene glycol aqueous solution extract 0.7 (7) Purified water 42.8 (8) Fragrance 0.2 Production method: (1) to (8) Knead and homogenize the components.

Example 14 Mouthwash (1) Ethanol 7.0 (% by weight) (2) Glycerin 15.0 (3) Polyoxyethylene (50EO) hydrogenated castor oil 2.0 (4) Saccharin sodium 0.5 (5) Bodaiju flower ・ 50% by weight 1,3-butylene glycol aqueous solution extract 0.5 (6) Purified water 74.5 (7) Fragrance 0.5 Production method: Components (1) to (7) are added sequentially. Mix and homogenize.

Next, the above Examples 1 to 14 were tested for antibacterial activity against various bacteria and fungi in accordance with the above method. At the same time, those without the extract of the genus Linus according to the formulation of each example were also prepared, and each was used as a comparative example. However, the antibacterial activity test of Comparative Example 4 was not performed because the formulation was water-free and a linden plant extract was added to prevent secondary contamination during use.

[0051]

[Table 5]

As shown in Table 5, Examples 1 to 1
4 showed antibacterial activity against all bacteria and fungi. However, in Comparative Examples 1 to 14 in which no extract of the genus Lindenium was blended, none of the bacterial species passed.

[0053]

As described in detail above, an external preparation for skin containing an extract of a plant of the genus Lindus as an antibacterial active ingredient is extremely safe, and various kinds of bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Excellent antibacterial action against Candida and black mold.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification code FI A61K 7/48 A61K 7/48

Claims (5)

[Claims] 1. An external preparation for skin containing an extract of a plant of the genus Linus as an antibacterial component. 2. The external preparation for skin according to claim 1, comprising a polar solvent extract of a plant belonging to the genus Lindus as an antibacterial component. 3. The plant of the genus Lindensis is a Thu-daidai ( Til)
ia cordata Mill., Tilia ulmifolia Scop., Tilia par
vifolia Ehrh.), Natsubodaiju ( Tilia platyphyllos)
3. The external preparation for skin according to claim 1 or 2, wherein the external preparation is one or more selected from Scop., Tilia grandifolia Ehrh.) And European linden ( Tilia europaea L.). 4. The external preparation for skin according to claim 1, which comprises an extract of a flower of a genus Linus as an antibacterial component. 5. The external preparation for skin according to claim 1, which comprises an extract of a 1,3-butylene glycol aqueous solution of 50% by weight of a plant of the genus Lindus as an antibacterial component.