JP2845961B2 - How to promote plant growth - Google Patents

Description

The present invention relates to a method for promoting plant growth, and more particularly, to a method for promoting plant growth using a deproteinized solution produced when extracting leaf proteins from alfalfa stems and leaves.

[Problems to be solved by conventional technology and invention]

Alfalfa (scientific name: Medicago sativa L., U.S. name: alf
alfa, English name: lucerne, m
edicks (Japanese name: purple coconut palm) is a leguminous plant whose bean can be eaten as it is, and in Europe it is called "king of sprouts" and "father of food". Also, leaf proteins are extracted from foliage and used as food and concentrated feed.

On the other hand, it is known that squeezing liquid obtained by squeezing alfalfa and applying fermented boca fertilized by mixing mountain soil and clay with organic fertilizer promotes plant growth and has an effect of increasing yield ( Modern Agriculture Vol. 67 No. 6, 170-173, 1988 and Modern Agriculture Vol. 67 No. 10, 192-195, 1988). Furthermore, green juice excluding the residual portion of the squeezed liquid contains useful substances such as green leaf protein.
It is conceivable to use a deproteinized supernatant (hereinafter referred to as brown juice) discharged when the juice is heated and then filtered to extract the green leaf protein. However, the active ingredient of this brown juice has not been elucidated, and its use has not yet been sufficiently studied. Furthermore, there is no known method for promoting plant growth.

[Means for solving the problem]

The present inventors have found that brown juice obtained from alfalfa has the effect of promoting the growth of various vegetables and other plants and increasing the yield, and completed the present invention.

That is, the present invention relates to a method wherein the alfalfa-derived green juice is heated, then filtered, and the alfalfa green leaf deproteinized supernatant discharged when extracting green leaf protein is 0.01% (v / v) to 0.1% of the nutrient solution. % (V / v). A method for promoting plant growth.

The alfalfa green leaf deproteinized supernatant used in the present invention, ie, brown juice, is usually discharged when extracting green leaf protein from alfalfa. That is, it refers to a deproteinized supernatant obtained by squeezing the cut alfalfa into green juice and press cake, heating the obtained green juice, and filtering to remove green leaf proteins. Alternatively, dried alfalfa squeezed by adding water can also be used. Processes such as alfalfa squeezing and protein extraction from green juice may be performed by a conventional method, and there are no restrictions on conditions such as pressure, temperature, and filtration method. The following is an example of the preparation of brown juice.

The cut alfalfa is squeezed with a squeezing machine to obtain green juice. The protein is coagulated by inserting steam at 130 ° C., and then centrifuged to obtain the desired brown juice. In addition, in order to coagulate and remove the contained protein, an acid treatment such as adding an acid to the green juice or performing lactic acid fermentation is performed.
Although a heating treatment of up to 95 ° C. is performed, brown juice obtained by such a treatment can be used in the present invention.

Next, the composition of the components of the brown juice is shown in Table 1.

Table 1 Crude protein 0.20 to 1.50% (W / W) Crude sugar 0.30 to 2.00% (W / W) Crude ash 0.30 to 2.00% (W / W) Crude fat 0.03 to 0.20% (W / W) Total nitrogen 0.03 to 0.20% (W / W) Dry matter 1.50 to 6.00% (W / W) pH 4.0 to 6.0 Plants suitable for the method of the present invention include cereals such as rice, vegetables such as honeybee, salad vegetables, large leaves, eggplant,
Examples include tomato, radish, alfalfa as sprouts and pasture, fruits such as strawberries, flowers and plants such as amaryllis.

In the present invention, the cultivation method of each of these plants, for example, when cultivated in soil cultivation or hydroponic cultivation, by adding the above-mentioned brown juice to the nutrient solution, it is possible to promote the growth of the plant . Steam brown juice is added at a ratio of 0.01% (v / v) to 0.1% (v / v) based on the nutrient solution.

In the present invention, brown juice may be added to soil or nutrient solution in a required amount, and may be added to fertilizers and the like used in ordinary cultivation at the time of use or may be mixed in advance with the fertilizer. And may be applied. In addition, the cultivation conditions may be normal conditions suitable for each plant, and there is no need to set special conditions for using brown juice.

〔Example〕

 Next, the present invention will be described with reference to examples.

Example 1 Thirty salad vegetables (commercially available) and 30 honeybees (commercially available) were cultivated in a greenhouse using a commercially available hydroponic cultivation apparatus (trade name: Home Hyponica 301, Kyowa Co., Ltd.).

The cultivation method followed the Hyponica cultivation method. The brown juice added area contains 0.01% of brown juice (v /
v) Two months after planting the added seedlings, the whole was harvested. Table 2 shows the results and the growth effect (comparison with the control group as 100%).

Example 2 In the same manner as in Example 1, rice (cultivar name: Nihonbare) was hydroponically cultivated and seedlings were planted. After observation of foliage 2 months later, the brown juice added plot (0.01% (v / v) )) Showed a remarkable growth promoting effect.

Example 3 Thirty large leaves (cultivar names: Aoshishiso, Takii seedlings) were cultivated in a greenhouse by submerged hydroponic cultivation (using rock wool for plant support). The fertilizer used was Otsuka House Fertilizer (Otsuka Chemical Co., Ltd.), and the nutrient solution was 0.9 units of B formulation standard solution (see Mitsuo Shikikawa, Encyclopedia of Hydroponics, Tominkai Shuppan, 1987).
Was used under the conditions of a liquid depth of a cultivation bed of 3 cm and a nutrient solution circulation rate of 4 / min. The brown juice addition group added brown juice to the nutrient solution to a concentration of 0.025% (v / v). One month after planting the seedlings, the length is 10 every 3 days.
Leaves larger than 8 cm in width and 8 cm in width were harvested for one month. Table 3 shows the yield and the growth effect (when the control was set to 100%).

Example 4 30 eggplants (variety name: Kuroyo, commercially available seedlings) were cultivated in a greenhouse using a commercially available hydroponic cultivation device (trade name: Home Hyponica 302, Kyowa Co., Ltd.). The cultivation method conformed to the Hyponica cultivation method. The brown juice addition group added 0.025% (v / v) or 0.05% (v / v) of brown juice to the nutrient solution. One month after planting the seedlings, the stem length was measured. This result and the growth effect (when the control group is 100%)
Are shown in Table 4.

Example 5 A commercially available hydroponic cultivation apparatus (trade name: Home Hyponica 302, Kyowa Co., Ltd.) was used for 30 tomatoes (variety name: Fukuju, commercial seedling).
And cultivated in a greenhouse. Cultivation method and fertilizer conformed to Hyponica cultivation method. The brown juice added area contains 0.025% (v / v) or 0.05% of brown juice in the nutrient solution
(V / v) was added. One month after planting the seedlings, the stem length was measured. Table 5 shows the results and the growth effect (when the control was set to 100%).

Example 6 Thirty strawberries (variety name: Toyooka, commercially available seedlings) were cultivated in a greenhouse using a commercially available hydroponic cultivation apparatus (trade name: HiFlolot Mini, Affinity Plastics Co., Ltd.). Cultivation method and fertilizer were based on the affinity floating root method. The Brown Juice-added area added 0.025% (v / v)
v) or 0.05% (v / v) was added. 10 after planting seedlings
After 0 days, the fruit set was harvested for 14 days. This result and the growth effect (when the control group is 100%)
Are shown in Table 6.

Example 7 Alfalfa (breed name: alfalfa for sprouts,
Takii seedlings (30 seedlings) were cultivated in a greenhouse using a commercially available hydroponic cultivation apparatus (trade name: High Float Mini, Affinity Plastics Co., Ltd.). Cultivation method and fertilizer were based on the affinity floating root method. The Brown Juice Addition District uses Brown
Juice 0.025% (v / v), 0.05% (v / v) or 0.1%
(V / v) was added. The stem length and yield were measured 45 days after planting the raised seedlings. This result and the growth effect (control
Table 7).

Example 8 Seeds of cracked radish (cultivar name: Kairai radish, Tohoku seedling) were cultivated in 30 plastic containers (sponge on the bottom) 6 cm in diameter and 5 cm in height, each containing 5 g. 20 for sponge
After cultivation in a dark place (25 ° C.) for 2 days, water was drained, and 20 ml of a 800-fold diluted solution of a commercially available fertilizer (trade name: Highlyzer M, Affinity Plastics Co., Ltd.) was added as a nutrient solution. .
In the brown juice addition area, 0.025% (v / v) of brown juice was added to the nutrient solution. This was further cultivated in a dark place (25 ° C.) for 8 days, and then greened in a light place (25 ° C.) for 1 day, and then the whole was harvested. Table 8 shows the average yield per container and the growth effect (when the control was set to 100%).

Example 9 A commercial hydroponic cultivation apparatus (trade name: High Flow Lot Mini, Affinity Plastic Co., Ltd.) was used for 30 Mitsuba (commercial products).
And cultivated in a greenhouse. Cultivation method and fertilizer were based on the affinity floating root method. The brown juice added area contains 0.025% (v / v) or 0.05% (v / v)
v) Added. One month after planting the raised seedlings, the entire amount was harvested. This result and the growth effect (100
%) Is shown in Table 9.

Example 10 30 tomatoes (variety name: large fukuju, commercial seedling) were treated with urea
It was planted in a pot containing 10 kg of culture soil (air-dry weight) mixed with a fertilizer of 2 g, 6 g of superphosphate, and 2 g of potassium chloride, and cultivated outdoors. In the brown juice added section, 5 ml of brown juice was added to the fertilizer and then mixed. One month after the planting, the stem length was measured. This result and the growth effect (control
Table 10).

Example 11 30 amaryllis (commercially available) bulbs were planted in a planter. The cultivation bed uses water moss, and the nutrient solution is Highlyzer M
(Trade name: Affinity Plastic Co., Ltd.) was used at a 2000-fold dilution. In the brown juice addition zone, 0.025% (v / v) and 0.050% (v / v) of brown juice were added to the nutrient solution. The cells were cultured in a greenhouse, and the number of days required for flowering was measured.
Table 11 shows the average days required for flowering.

[Effects of the Invention] According to the present invention, it is possible to promote the growth of plants inexpensively and reliably using brown juice that has been discharged as waste, thereby increasing the yield and shortening the cultivation period. To be peeled off.

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Claims (1)

(57) [Claims] 1. After heating a green juice derived from alfalfa, filtering it, and removing the alfalfa green leaf deproteinized supernatant discharged when extracting green leaf protein, 0.01% (v / v) to 0.1% of the nutrient solution. (V / v) a method for promoting plant growth.