JP2025102784A5 - - Google Patents

Info

Publication number
JP2025102784A5
JP2025102784A5 JP2025034660A JP2025034660A JP2025102784A5 JP 2025102784 A5 JP2025102784 A5 JP 2025102784A5 JP 2025034660 A JP2025034660 A JP 2025034660A JP 2025034660 A JP2025034660 A JP 2025034660A JP 2025102784 A5 JP2025102784 A5 JP 2025102784A5
Authority
JP
Japan
Prior art keywords
fusion protein
cas9 polypeptide
terminal fragment
cas9
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2025034660A
Other languages
English (en)
Japanese (ja)
Other versions
JP2025102784A (ja
Filing date
Publication date
Priority claimed from JP2021544294A external-priority patent/JP7646552B2/ja
Application filed filed Critical
Publication of JP2025102784A publication Critical patent/JP2025102784A/ja
Publication of JP2025102784A5 publication Critical patent/JP2025102784A5/ja
Pending legal-status Critical Current

Links

JP2025034660A 2019-01-31 2025-03-05 低減された非標的脱アミノ化を有する核酸塩基エディターおよび核酸塩基エディターの特徴づけのためのアッセイ Pending JP2025102784A (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962799702P 2019-01-31 2019-01-31
US62/799,702 2019-01-31
JP2021544294A JP7646552B2 (ja) 2019-01-31 2020-01-31 低減された非標的脱アミノ化を有する核酸塩基エディターおよび核酸塩基エディターの特徴づけのためのアッセイ
PCT/US2020/016285 WO2020160514A1 (en) 2019-01-31 2020-01-31 Nucleobase editors having reduced non-target deamination and assays for characterizing nucleobase editors

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2021544294A Division JP7646552B2 (ja) 2019-01-31 2020-01-31 低減された非標的脱アミノ化を有する核酸塩基エディターおよび核酸塩基エディターの特徴づけのためのアッセイ

Publications (2)

Publication Number Publication Date
JP2025102784A JP2025102784A (ja) 2025-07-08
JP2025102784A5 true JP2025102784A5 (enExample) 2025-10-31

Family

ID=71842366

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2021544294A Active JP7646552B2 (ja) 2019-01-31 2020-01-31 低減された非標的脱アミノ化を有する核酸塩基エディターおよび核酸塩基エディターの特徴づけのためのアッセイ
JP2025034660A Pending JP2025102784A (ja) 2019-01-31 2025-03-05 低減された非標的脱アミノ化を有する核酸塩基エディターおよび核酸塩基エディターの特徴づけのためのアッセイ

Family Applications Before (1)

Application Number Title Priority Date Filing Date
JP2021544294A Active JP7646552B2 (ja) 2019-01-31 2020-01-31 低減された非標的脱アミノ化を有する核酸塩基エディターおよび核酸塩基エディターの特徴づけのためのアッセイ

Country Status (8)

Country Link
US (1) US20220098572A1 (enExample)
EP (1) EP3918078A4 (enExample)
JP (2) JP7646552B2 (enExample)
KR (1) KR20210121113A (enExample)
CN (1) CN114040977B (enExample)
AU (1) AU2020215730A1 (enExample)
CA (1) CA3127493A1 (enExample)
WO (1) WO2020160514A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180250424A1 (en) 2014-10-10 2018-09-06 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
CA3100019A1 (en) 2018-05-11 2019-11-14 Beam Therapeutics Inc. Methods of substituting pathogenic amino acids using programmable base editor systems
WO2020051562A2 (en) 2018-09-07 2020-03-12 Beam Therapeutics Inc. Compositions and methods for improving base editing
BR112022017715A2 (pt) 2020-03-04 2022-11-16 Flagship Pioneering Innovations Vi Llc Métodos e composições para modular um genoma
US20230159913A1 (en) 2020-04-28 2023-05-25 The Broad Institute, Inc. Targeted base editing of the ush2a gene
EP4209589A4 (en) * 2020-09-04 2024-09-25 National University Corporation Kobe University Miniaturized cytidine deaminase-containing complex for modifying double-stranded dna
CN116601293A (zh) * 2020-12-07 2023-08-15 中国科学院动物研究所 工程化的Cas效应蛋白及其使用方法
CN112195164B (zh) * 2020-12-07 2021-04-23 中国科学院动物研究所 工程化的Cas效应蛋白及其使用方法
KR20240004276A (ko) * 2021-03-26 2024-01-11 빔 테라퓨틱스, 인크. 아데노신 데아미나아제 변이체 및 이의 용도
EP4367227A4 (en) * 2021-07-06 2025-04-30 Prime Medicine, Inc. Compositions and methods for efficient genome editing
MX2024002927A (es) 2021-09-08 2024-05-29 Flagship Pioneering Innovations Vi Llc Metodos y composiciones para modular un genoma.
WO2023225451A1 (en) * 2022-05-14 2023-11-23 The Regents Of The University Of California Genome editing systems for multiplexing point mutation introduction in living cells
WO2025119306A1 (zh) * 2023-12-06 2025-06-12 北京齐禾生科生物科技有限公司 优化工程化t细胞的碱基编辑系统及其应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015089486A2 (en) * 2013-12-12 2015-06-18 The Broad Institute Inc. Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems
US20150166984A1 (en) * 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
DK3234200T3 (da) * 2014-12-20 2021-08-23 Arc Bio Llc Fremgangsmåde til målrettet depletering af nukleinsyrer ved anvendelse af CRISPR/CAS-systemproteiner
WO2016123243A1 (en) * 2015-01-28 2016-08-04 The Regents Of The University Of California Methods and compositions for labeling a single-stranded target nucleic acid
US10392607B2 (en) * 2015-06-03 2019-08-27 The Regents Of The University Of California Cas9 variants and methods of use thereof
RU2752834C2 (ru) * 2015-06-18 2021-08-09 Те Брод Инститьют, Инк. Мутации фермента crispr, уменьшающие нецелевые эффекты
US11008555B2 (en) * 2015-09-17 2021-05-18 The Regents Of The University Of California Variant Cas9 polypeptides comprising internal insertions
US12043852B2 (en) * 2015-10-23 2024-07-23 President And Fellows Of Harvard College Evolved Cas9 proteins for gene editing
CA3032699A1 (en) * 2016-08-03 2018-02-08 President And Fellows Of Harvard College Adenosine nucleobase editors and uses thereof
US11840694B2 (en) * 2016-10-17 2023-12-12 Nanyang Technological University Truncated CRISPR-Cas proteins for DNA targeting
WO2018119359A1 (en) * 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3601562A1 (en) * 2017-03-23 2020-02-05 President and Fellows of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018213726A1 (en) * 2017-05-18 2018-11-22 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
CA3064601A1 (en) * 2017-06-26 2019-01-03 The Broad Institute, Inc. Crispr/cas-adenine deaminase based compositions, systems, and methods for targeted nucleic acid editing

Similar Documents

Publication Publication Date Title
JP2025102784A5 (enExample)
US20220213507A1 (en) Aav delivery of nucleobase editors
US20250115884A1 (en) TAL-Tet1 Fusion Proteins and Methods of Use Thereof
US12157760B2 (en) Base editors and uses thereof
US11840694B2 (en) Truncated CRISPR-Cas proteins for DNA targeting
AU2018272067B2 (en) Base editors with improved precision and specificity
EP3178935B1 (en) Genome editing using campylobacter jejuni crispr/cas system-derived rgen
CN110612353A (zh) 经由抑制性tRNAs和脱氨酶对突变进行RNA靶向
JP2017018125A5 (enExample)
CN108291218A (zh) 核酸酶非依赖性靶向基因编辑平台及其用途
JP2016537008A5 (enExample)
CN110520163A (zh) 独立于dna双链断裂的靶向基因编辑平台及其用途
EP2776560A2 (en) Endonuclease for genome editing
JPWO2020168132A5 (enExample)
JPWO2021050571A5 (enExample)
JPWO2020160514A5 (enExample)
Li et al. Site-directed RNA editing by harnessing ADARs: advances and challenges
CN118147120A (zh) 胞苷脱氨酶及碱基编辑器
JPWO2020181102A5 (enExample)
CN103764820A (zh) 序列特异性工程改造的核糖核酸酶h和用于确定dna-rna杂交物结合蛋白序列偏好的方法
JPWO2020219908A5 (enExample)
US20200263204A1 (en) Composition for treating hemophilia a by crispr/cas system of reverting fviii gene inversion
KR20250148471A (ko) 핵산 결합 단백질 및 SsdA를 포함하는 융합단백질 및 이의 용도
KR20200125191A (ko) 표적 특이적 crispr 변이체
JPWO2022204574A5 (enExample)