CN114040977B - 非靶脱氨反应减低的核碱基编辑器和用于定性核碱基编辑器的测定 - Google Patents

非靶脱氨反应减低的核碱基编辑器和用于定性核碱基编辑器的测定 Download PDF

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CN114040977B
CN114040977B CN202080026678.0A CN202080026678A CN114040977B CN 114040977 B CN114040977 B CN 114040977B CN 202080026678 A CN202080026678 A CN 202080026678A CN 114040977 B CN114040977 B CN 114040977B
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cas9
amino acid
deaminase
domain
fusion protein
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CN114040977A (zh
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I·斯雷梅克
J·M·格尔克
N·戈代尔利
Y·于
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Bim Medical Co ltd
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
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    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)

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CN202080026678.0A 2019-01-31 2020-01-31 非靶脱氨反应减低的核碱基编辑器和用于定性核碱基编辑器的测定 Active CN114040977B (zh)

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Application Number Priority Date Filing Date Title
US201962799702P 2019-01-31 2019-01-31
US62/799,702 2019-01-31
PCT/US2020/016285 WO2020160514A1 (en) 2019-01-31 2020-01-31 Nucleobase editors having reduced non-target deamination and assays for characterizing nucleobase editors

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CN114040977A CN114040977A (zh) 2022-02-11
CN114040977B true CN114040977B (zh) 2024-09-03

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US (1) US20220098572A1 (enExample)
EP (1) EP3918078A4 (enExample)
JP (2) JP7646552B2 (enExample)
KR (1) KR20210121113A (enExample)
CN (1) CN114040977B (enExample)
AU (1) AU2020215730A1 (enExample)
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EP3204496A1 (en) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
US12133884B2 (en) 2018-05-11 2024-11-05 Beam Therapeutics Inc. Methods of substituting pathogenic amino acids using programmable base editor systems
WO2020051562A2 (en) 2018-09-07 2020-03-12 Beam Therapeutics Inc. Compositions and methods for improving base editing
AU2021230546A1 (en) 2020-03-04 2022-10-13 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
US20230159913A1 (en) 2020-04-28 2023-05-25 The Broad Institute, Inc. Targeted base editing of the ush2a gene
BR112023003972A2 (pt) * 2020-09-04 2023-04-18 Univ Kobe Nat Univ Corp Complexos de um módulo de reconhecimento de sequência de ácido nucleico ligado a uma desaminase e de um fragmento amino-terminal de um módulo de reconhecimento de sequência de ácido nucleico, uma desaminase e um fragmento carboxi-terminal de um módulo de reconhecimento de sequência de ácido nucleico ligado ao mesmo, ácido nucleico, vetor, e, método para alterar um sítio alvo de um ácido desoxirribonucleico de fita dupla de uma célula
CN112195164B (zh) * 2020-12-07 2021-04-23 中国科学院动物研究所 工程化的Cas效应蛋白及其使用方法
CN116601293A (zh) * 2020-12-07 2023-08-15 中国科学院动物研究所 工程化的Cas效应蛋白及其使用方法
AU2022246176A1 (en) * 2021-03-26 2023-10-05 Beam Therapeutics Inc. Adenosine deaminase variants and uses thereof
AU2022306377A1 (en) * 2021-07-06 2024-01-25 Prime Medicine, Inc. Compositions and methods for efficient genome editing
CA3231677A1 (en) 2021-09-08 2023-03-16 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
US20250361530A1 (en) * 2022-05-14 2025-11-27 The Regents Of The University Of California Genome editing systems for multiplexing point mutation introduction in living cells
CN119591727B (zh) * 2023-12-06 2025-09-30 北京齐禾生科生物科技有限公司 优化的qbe碱基编辑系统及其应用

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CN108290933A (zh) * 2015-06-18 2018-07-17 布罗德研究所有限公司 降低脱靶效应的crispr酶突变
CN108699116A (zh) * 2015-10-23 2018-10-23 哈佛大学的校长及成员们 用于基因编辑的演化的cas9蛋白

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US11053481B2 (en) * 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
EP3080271B1 (en) * 2013-12-12 2020-02-12 The Broad Institute, Inc. Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems
CA2971444A1 (en) * 2014-12-20 2016-06-23 Arc Bio, Llc Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
WO2016123243A1 (en) * 2015-01-28 2016-08-04 The Regents Of The University Of California Methods and compositions for labeling a single-stranded target nucleic acid
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CN108699116A (zh) * 2015-10-23 2018-10-23 哈佛大学的校长及成员们 用于基因编辑的演化的cas9蛋白

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EP3918078A1 (en) 2021-12-08
JP2025102784A (ja) 2025-07-08
CN114040977A (zh) 2022-02-11
JP2022519507A (ja) 2022-03-24
JP7646552B2 (ja) 2025-03-17
WO2020160514A1 (en) 2020-08-06
CA3127493A1 (en) 2020-08-06
AU2020215730A1 (en) 2021-07-29
KR20210121113A (ko) 2021-10-07
EP3918078A4 (en) 2022-12-14
US20220098572A1 (en) 2022-03-31

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