JP2024160269A5 - - Google Patents

Download PDF

Info

Publication number
JP2024160269A5
JP2024160269A5 JP2024124506A JP2024124506A JP2024160269A5 JP 2024160269 A5 JP2024160269 A5 JP 2024160269A5 JP 2024124506 A JP2024124506 A JP 2024124506A JP 2024124506 A JP2024124506 A JP 2024124506A JP 2024160269 A5 JP2024160269 A5 JP 2024160269A5
Authority
JP
Japan
Prior art keywords
nucleic acid
target nucleic
sequence
barcode
fragments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2024124506A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024160269A (ja
Filing date
Publication date
Priority claimed from JP2020204004A external-priority patent/JP7127104B2/ja
Application filed filed Critical
Publication of JP2024160269A publication Critical patent/JP2024160269A/ja
Publication of JP2024160269A5 publication Critical patent/JP2024160269A5/ja
Pending legal-status Critical Current

Links

JP2024124506A 2014-10-17 2024-07-31 連続性を維持した転位 Pending JP2024160269A (ja)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201462065544P 2014-10-17 2014-10-17
US62/065,544 2014-10-17
US201562157396P 2015-05-05 2015-05-05
US62/157,396 2015-05-05
US201562242880P 2015-10-16 2015-10-16
US62/242,880 2015-10-16
JP2020204004A JP7127104B2 (ja) 2014-10-17 2020-12-09 連続性を維持した転位
JP2022129859A JP7532455B2 (ja) 2014-10-17 2022-08-17 連続性を維持した転位

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2022129859A Division JP7532455B2 (ja) 2014-10-17 2022-08-17 連続性を維持した転位

Publications (2)

Publication Number Publication Date
JP2024160269A JP2024160269A (ja) 2024-11-13
JP2024160269A5 true JP2024160269A5 (https=) 2024-11-21

Family

ID=55747561

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2017520884A Active JP6808617B2 (ja) 2014-10-17 2015-10-16 連続性を維持した転位
JP2020204004A Active JP7127104B2 (ja) 2014-10-17 2020-12-09 連続性を維持した転位
JP2022129859A Active JP7532455B2 (ja) 2014-10-17 2022-08-17 連続性を維持した転位
JP2024124506A Pending JP2024160269A (ja) 2014-10-17 2024-07-31 連続性を維持した転位

Family Applications Before (3)

Application Number Title Priority Date Filing Date
JP2017520884A Active JP6808617B2 (ja) 2014-10-17 2015-10-16 連続性を維持した転位
JP2020204004A Active JP7127104B2 (ja) 2014-10-17 2020-12-09 連続性を維持した転位
JP2022129859A Active JP7532455B2 (ja) 2014-10-17 2022-08-17 連続性を維持した転位

Country Status (10)

Country Link
US (3) US11873480B2 (https=)
JP (4) JP6808617B2 (https=)
KR (2) KR102643955B1 (https=)
AU (2) AU2015331739B2 (https=)
BR (2) BR122021026781B1 (https=)
CA (1) CA2964799A1 (https=)
IL (3) IL299976B2 (https=)
RU (2) RU2736728C2 (https=)
SG (2) SG11201703139VA (https=)
WO (1) WO2016061517A2 (https=)

Families Citing this family (182)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US10457936B2 (en) 2011-02-02 2019-10-29 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA3216609C (en) 2012-08-14 2024-05-14 10X Genomics, Inc. Microcapsule compositions and methods
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
WO2014142850A1 (en) 2013-03-13 2014-09-18 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
MX2016016904A (es) 2014-06-26 2017-03-27 10X Genomics Inc Analisis de secuencias de acidos nucleicos.
CA2956925C (en) 2014-08-01 2024-02-13 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
US11873480B2 (en) 2014-10-17 2024-01-16 Illumina Cambridge Limited Contiguity preserving transposition
MX2017005267A (es) 2014-10-29 2017-07-26 10X Genomics Inc Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana.
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
NZ734854A (en) 2015-02-17 2022-11-25 Dovetail Genomics Llc Nucleic acid sequence assembly
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
BR112017018054A2 (pt) 2015-02-24 2018-07-24 10X Genomics Inc métodos para a cobertura de sequências de ácidos nucleicos direcionadas
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
US11807896B2 (en) 2015-03-26 2023-11-07 Dovetail Genomics, Llc Physical linkage preservation in DNA storage
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
CN107580632B (zh) 2015-04-23 2021-12-28 贝克顿迪金森公司 用于全转录组扩增的方法和组合物
WO2016189331A1 (en) * 2015-05-28 2016-12-01 Illumina Cambridge Limited Surface-based tagmentation
WO2017004612A1 (en) 2015-07-02 2017-01-05 Arima Genomics, Inc. Accurate molecular deconvolution of mixtures samples
WO2017034970A1 (en) * 2015-08-21 2017-03-02 The General Hospital Corporation Combinatorial single molecule analysis of chromatin
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
CA3002740A1 (en) 2015-10-19 2017-04-27 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
WO2017120531A1 (en) 2016-01-08 2017-07-13 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
US20190169602A1 (en) * 2016-01-12 2019-06-06 Seqwell, Inc. Compositions and methods for sequencing nucleic acids
JP6735348B2 (ja) 2016-02-11 2020-08-05 10エックス ジェノミクス, インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
JP7441003B2 (ja) 2016-02-23 2024-02-29 ダブテイル ゲノミクス エルエルシー ゲノムアセンブリのためのフェージングされたリードセットの生成とハプロタイプフェージング
US20170283864A1 (en) 2016-03-31 2017-10-05 Agilent Technologies, Inc. Use of transposase and y adapters to fragment and tag dna
AU2017259794B2 (en) 2016-05-02 2023-04-13 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197300A1 (en) 2016-05-13 2017-11-16 Dovetail Genomics Llc Recovering long-range linkage information from preserved samples
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10894990B2 (en) 2016-05-17 2021-01-19 Shoreline Biome, Llc High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP3485034B1 (en) * 2016-07-12 2021-08-18 Kapa Biosystems, Inc. System and method for transposase-mediated amplicon sequencing
CN117822128A (zh) * 2016-07-22 2024-04-05 俄勒冈健康与科学大学 单细胞全基因组文库及制备其的组合索引方法
CN109804068A (zh) * 2016-08-10 2019-05-24 哈佛学院董事及会员团体 从头组装条码化的基因组dna片段的方法
CN116445593A (zh) * 2016-08-10 2023-07-18 格里尔公司 测定一生物样品的一甲基化图谱的方法
WO2018057779A1 (en) * 2016-09-23 2018-03-29 Jianbiao Zheng Compositions of synthetic transposons and methods of use thereof
CA3034924C (en) 2016-09-26 2025-11-18 Becton, Dickinson And Company MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES
WO2018118971A1 (en) * 2016-12-19 2018-06-28 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201622219D0 (en) 2016-12-23 2017-02-08 Cs Genetics Ltd Methods and reagents for molecular barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
CN110214186B (zh) 2017-01-30 2023-11-24 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
US10968447B2 (en) * 2017-01-31 2021-04-06 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
WO2018152244A1 (en) * 2017-02-14 2018-08-23 Seqwell, Inc. Compositions and methods for sequencing nucleic acids
KR20250143875A (ko) 2017-02-21 2025-10-02 일루미나, 인코포레이티드 링커를 갖는 고정된 트랜스포좀을 사용한 태그먼트화
KR20200004335A (ko) 2017-05-05 2020-01-13 스키피오 바이오사이언스 하이드로겔 내의 별개의 생물학적 단위를 트래핑하고 바코딩하는 방법
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) * 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
FI3981884T3 (fi) * 2017-06-07 2023-09-21 Univ Oregon Health & Science Yksittäissolujen kokogenomikirjastoja metylaatiosekvensointia varten
WO2019060722A2 (en) 2017-09-22 2019-03-28 X Gen Us Co. METHODS AND COMPOSITIONS FOR USE IN PREPARING POLYNUCLEOTIDES
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019076768A1 (en) * 2017-10-16 2019-04-25 Tervisetehnoloogiate Arenduskeskus As METHOD AND KIT FOR PREPARING DNA BANK
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN114525273B (zh) 2017-10-27 2025-01-28 10X基因组学有限公司 用于样品制备和分析的方法和系统
MX2020004559A (es) 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
EP3704247B1 (en) 2017-11-02 2023-01-04 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11873481B2 (en) 2017-11-21 2024-01-16 Arima Genomics, Inc. Preserving spatial-proximal contiguity and molecular contiguity in nucleic acid templates
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
CN118818037A (zh) 2017-12-12 2024-10-22 10X基因组学有限公司 用于单细胞处理的系统和方法
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
WO2019152543A1 (en) 2018-01-31 2019-08-08 Dovetail Genomics, Llc Sample prep for dna linkage recovery
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
KR20200026250A (ko) 2018-04-20 2020-03-10 일루미나, 인코포레이티드 단일 세포를 캡슐화하는 방법, 캡슐화된 세포 및 이의 용도
EP3784797A1 (en) 2018-04-27 2021-03-03 X Gen US Co. Methods and compositions for preparing polynucleotides
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
CN120624607A (zh) 2018-05-08 2025-09-12 深圳华大智造科技股份有限公司 用于准确且经济高效的测序、单体型分型和组装的基于单管珠粒的dna共条形码化
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
IL271454B2 (en) * 2018-05-17 2025-04-01 Illumina Inc Rapid single-cell genetic sequencing with low Aggregation bias
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3830289B1 (en) 2018-08-03 2026-02-11 10X Genomics, Inc. Methods to minimize barcode exchange
EP4249651B1 (en) 2018-08-20 2025-01-29 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
CN112739829A (zh) * 2018-09-27 2021-04-30 深圳华大生命科学研究院 测序文库的构建方法和得到的测序文库及测序方法
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
EP3870704A4 (en) * 2018-10-25 2023-01-11 Illumina, Inc. METHODS AND COMPOSITIONS FOR IDENTIFICATION OF LIGANDS ON ARRAYS WITH INDEXES AND BARCODES
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
MY204975A (en) * 2019-01-11 2024-09-25 Illumina Cambridge Ltd Complex surface-bound transposome complexes
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
TWI857000B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 流體槽以及將複合物引入至流體槽之方法
TWI873119B (zh) 2019-01-29 2025-02-21 美商伊路米納有限公司 流通槽及製備其之方法
WO2020157684A1 (en) 2019-01-29 2020-08-06 Mgi Tech Co., Ltd. High coverage stlfr
TWI857001B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 定序套組
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
AU2020220461B2 (en) 2019-02-14 2026-02-12 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Haplotagging - haplotype phasing and single-tube combinatorial barcoding of nucleic acid molecules using bead-immobilized Tn5 transposase
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN113767178A (zh) 2019-03-11 2021-12-07 10X基因组学有限公司 用于处理光学标签化珠粒的系统和方法
CN113811619A (zh) 2019-03-27 2021-12-17 10X基因组学有限公司 用于处理来自细胞的rna的系统和方法
EP3963070A4 (en) 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
MX2021007774A (es) 2019-07-12 2021-08-05 Illumina Cambridge Ltd Preparacion de genoteca de acido nucleico usando electroforesis.
JP7667739B2 (ja) 2019-07-12 2025-04-23 イルミナ ケンブリッジ リミテッド 固体支持体上で固定化されたcrispr/cas9を使用する核酸シークエンシングライブラリを調製するための組成物及び方法
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
US20220298545A1 (en) * 2019-08-19 2022-09-22 Universal Sequencing Technology Corporation Methods and Compositions for Tracking Nucleic Acid Fragment Origin for Nucleic Acid Sequencing
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
AU2020351230A1 (en) * 2019-09-20 2022-01-20 Illumina, Inc. Methods and compositions for identifying ligands on arrays using indexes and barcodes
US12553044B2 (en) 2019-10-25 2026-02-17 Changping National Laboratory Methylation detection and analysis of mammalian DNA
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用
FI3931355T3 (fi) * 2019-12-02 2023-04-26 Illumina Cambridge Ltd Genomisen dna:n amplifioitujen säilyneen vierekkäisyyden kirjastofragmenttien aikaperusteinen klusterikuvaus
EP3927824A2 (en) 2019-12-19 2021-12-29 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
EP4090764A1 (en) 2020-01-13 2022-11-23 Becton, Dickinson and Company Cell capture using du-containing oligonucleotides
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
EP4103741A4 (en) * 2020-02-12 2024-03-20 Universal Sequencing Technology Corporation INTRACELLULAR AND SPATIAL BAR CODING PROCESSES
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
KR20230038145A (ko) * 2020-07-08 2023-03-17 일루미나, 인코포레이티드 트랜스포좀 담체로서의 비드
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
IL302207A (en) * 2020-10-21 2023-06-01 Illumina Inc Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
WO2022125997A1 (en) * 2020-12-11 2022-06-16 The Broad Institute, Inc. Method for duplex sequencing
CN114622286B (zh) * 2020-12-14 2026-04-24 中国农业大学 一种高通量的转录组测序文库构建方法及其应用
EP4263859A1 (en) 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
KR20230137394A (ko) * 2021-02-04 2023-10-04 일루미나, 인코포레이티드 트랜스포좀 결합된 비드 상의 긴 인덱싱된-연결된 판독 생성
CN120158497A (zh) 2021-02-23 2025-06-17 10X基因组学有限公司 基于探针的核酸和蛋白质分析
CN112950101B (zh) * 2021-05-13 2021-08-17 北京富通东方科技有限公司 一种基于产量预测的汽车工厂混流线排产方法
EP4430206A1 (en) 2021-11-10 2024-09-18 Encodia, Inc. Methods for barcoding macromolecules in individual cells
EP4272764A1 (en) 2022-05-03 2023-11-08 Scipio Bioscience Method of complexing biological units with particles
AU2023275773A1 (en) * 2022-05-26 2024-12-05 Illumina, Inc. Preparation of long read nucleic acid libraries
US20250305047A1 (en) * 2022-06-09 2025-10-02 The Regents Of The University Of California Single cell co-sequencing of dna methylation and rna
JP7825064B2 (ja) 2022-10-27 2026-03-05 住友化学株式会社 オリゴヌクレオチドの製造方法
JP2025542062A (ja) * 2022-12-20 2025-12-25 イルミナ インコーポレイテッド 標的ハイブリダイゼーションを向上させるための多価集合体
WO2025072677A1 (en) * 2023-09-29 2025-04-03 Illumina, Inc. Tagmentation methods and kits

Family Cites Families (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1989009835A1 (en) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
EP0379559B1 (en) 1988-06-24 1996-10-23 Amgen Inc. Method and reagents for detecting nucleic acid sequences
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
DE69118930T2 (de) 1990-01-26 1997-01-09 Abbott Lab Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
EP0754240B1 (en) 1994-02-07 2003-08-20 Beckman Coulter, Inc. Ligase/polymerase-mediated genetic bit analysis of single nucleotide polymorphisms and its use in genetic analysis
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
WO1995025180A1 (en) 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
KR20060099543A (ko) 1994-12-09 2006-09-19 임페리얼 컬리지 이노베이션스 리미티드 유전자의 동정
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US20020055100A1 (en) 1997-04-01 2002-05-09 Kawashima Eric H. Method of nucleic acid sequencing
FI103809B1 (fi) 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US20010046669A1 (en) 1999-02-24 2001-11-29 Mccobmie William R. Genetically filtered shotgun sequencing of complex eukaryotic genomes
US20050181440A1 (en) 1999-04-20 2005-08-18 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
WO2001034790A1 (en) 1999-11-08 2001-05-17 Eiken Kagaku Kabushiki Kaisha Method for synthesizing nucleic acid
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US6828098B2 (en) 2000-05-20 2004-12-07 The Regents Of The University Of Michigan Method of producing a DNA library using positional amplification based on the use of adaptors and nick translation
WO2002004680A2 (en) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
US6846658B1 (en) 2000-10-12 2005-01-25 New England Biolabs, Inc. Method for cloning and producing the Msel restriction endonuclease
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20040110191A1 (en) 2001-01-31 2004-06-10 Winkler Matthew M. Comparative analysis of nucleic acids using population tagging
US7138267B1 (en) 2001-04-04 2006-11-21 Epicentre Technologies Corporation Methods and compositions for amplifying DNA clone copy number
US6777187B2 (en) 2001-05-02 2004-08-17 Rubicon Genomics, Inc. Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates
GB0115194D0 (en) 2001-06-21 2001-08-15 Leuven K U Res & Dev Novel technology for genetic mapping
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
WO2004042078A1 (en) 2002-11-05 2004-05-21 The University Of Queensland Nucleotide sequence analysis by quantification of mutagenesis
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
DK1594971T3 (da) 2003-02-10 2011-05-23 Max Delbrueck Centrum Transposonbaseret målretningssystem
CA2528577C (en) 2003-06-20 2012-08-07 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2005042781A2 (en) 2003-10-31 2005-05-12 Agencourt Personal Genomics Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
US7727744B2 (en) 2004-03-30 2010-06-01 Epicentre Technologies Corporation Methods for obtaining directionally truncated polypeptides
US20080268507A1 (en) 2004-05-25 2008-10-30 Airbus Deutschland Gmbh Recombinant Dna Nicking Endonuclease and Uses Thereof
US7319142B1 (en) 2004-08-31 2008-01-15 Monsanto Technology Llc Nucleotide and amino acid sequences from Xenorhabdus and uses thereof
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7449297B2 (en) 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
US7601499B2 (en) 2005-06-06 2009-10-13 454 Life Sciences Corporation Paired end sequencing
JP5331476B2 (ja) 2005-06-15 2013-10-30 カリダ・ジェノミックス・インコーポレイテッド 遺伝子解析および化学解析用の単分子アレイ
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20070128610A1 (en) 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US20070172839A1 (en) 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
CA2643700A1 (en) 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
US20100022403A1 (en) 2006-06-30 2010-01-28 Nurith Kurn Methods for fragmentation and labeling of nucleic acids
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20090088331A1 (en) 2006-11-07 2009-04-02 Government Of The United States Of America, As Represented By The Secretary, Dept. Of Health And Influenza virus nucleic acid microarray and method of use
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US7948015B2 (en) 2006-12-14 2011-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
WO2008087040A2 (en) * 2007-01-19 2008-07-24 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
CN101848757B (zh) 2007-07-13 2013-12-04 里兰斯坦福初级大学理事会 用于改进的生物测定法的使用电场的方法和设备
CN101802223A (zh) 2007-08-15 2010-08-11 香港大学 用于高通量亚硫酸氢盐dna-测序的方法和组合物及其用途
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8852864B2 (en) 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
DK2279263T3 (da) 2008-04-30 2013-11-11 Integrated Dna Tech Inc Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer
KR20110025993A (ko) 2008-06-30 2011-03-14 바이오나노매트릭스, 인크. 단일-분자 전체 게놈 분석용 장치 및 방법
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
JP5794572B2 (ja) * 2008-10-24 2015-10-14 エピセンター テクノロジーズ コーポレイションEpicentre Technologies Corporation 核酸を修飾するためのトランスポゾン末端組成物及び方法
WO2010099301A2 (en) 2009-02-25 2010-09-02 The Johns Hopkins University Piggybac transposon variants and methods of use
WO2010115122A2 (en) 2009-04-03 2010-10-07 Illumina, Inc. Generation of uniform fragments of nucleic acids using patterned substrates
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
PL2539450T3 (pl) 2010-02-25 2016-08-31 Advanced Liquid Logic Inc Sposób wytwarzania bibliotek kwasu nukleinowego
MX2013001799A (es) * 2010-08-27 2013-05-20 Genentech Inc Metodos para la captura y secuenciacion de acido nucleico
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
RU2565550C2 (ru) 2010-09-24 2015-10-20 Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити Прямой захват, амплификация и секвенирование днк-мишени с использованием иммобилизированных праймеров
EP2625320B1 (en) * 2010-10-08 2019-03-27 President and Fellows of Harvard College High-throughput single cell barcoding
EP2632593B1 (en) 2010-10-27 2021-09-29 Illumina, Inc. Flow cells for biological or chemical analysis
US8829171B2 (en) * 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
CA2821299C (en) 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
EP3037536B1 (en) 2011-01-28 2019-11-27 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
US10457936B2 (en) * 2011-02-02 2019-10-29 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
WO2012108864A1 (en) 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
US9347059B2 (en) 2011-04-25 2016-05-24 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
NO2694769T3 (https=) * 2012-03-06 2018-03-03
SG11201405669XA (en) 2012-03-13 2014-10-30 Swift Biosciences Inc Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
US9968901B2 (en) 2012-05-21 2018-05-15 The Scripps Research Institute Methods of sample preparation
EP2852682B1 (en) 2012-05-21 2017-10-04 Fluidigm Corporation Single-particle analysis of particle populations
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9644199B2 (en) 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
AU2014214682B2 (en) * 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
CN105074010B (zh) * 2013-03-07 2018-04-17 积水医疗株式会社 甲基化dna 的检测方法
WO2014142850A1 (en) 2013-03-13 2014-09-18 Illumina, Inc. Methods and compositions for nucleic acid sequencing
EP4321628B1 (en) 2013-05-23 2025-09-03 The Board of Trustees of the Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
CA2935122C (en) 2013-12-30 2023-09-19 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
US11873480B2 (en) 2014-10-17 2024-01-16 Illumina Cambridge Limited Contiguity preserving transposition

Similar Documents

Publication Publication Date Title
JP2024160269A5 (https=)
AU2022201205B2 (en) Contiguity Preserving Transposition
US8986958B2 (en) Methods for generating target specific probes for solution based capture
AU2012212148B8 (en) Massively parallel contiguity mapping
EP3192869B1 (en) Isolated oligonucleotide and use thereof in nucleic acid sequencing
CN107969137B (zh) 接近性保留性转座
US20210155985A1 (en) Surface concatemerization of templates
CN110139931B (zh) 用于定相测序的方法和组合物
US20150050657A1 (en) Targeted enrichment and amplification of nucleic acids on a support
US20120015821A1 (en) Methods of Generating Gene Specific Libraries
WO2016040446A1 (en) Methods for selectively suppressing non-target sequences
WO2010030683A1 (en) Methods of generating gene specific libraries
JP2004524012A (ja) 固相支持体上における核酸の等温増幅
EP2242855A1 (en) Paired end sequencing
EP2728013A1 (en) Method for the simultaneous amplification of a plurality of different nucleic acid target sequences
JP2006506953A5 (https=)
US20230348961A1 (en) Ex-situ sequencing of rca product generated in-situ
EP4455307A1 (en) Ex-situ sequencing of rca product generated in-situ
RU2020137454A (ru) Транспозиция с сохранением сцепления генов
HK40068143A (en) Contiguity preserving transposition
CN119563034A (zh) 用于增强靶杂交的多价组装体