JP2024118709A - リパーゼ変異体 - Google Patents

リパーゼ変異体 Download PDF

Info

Publication number
JP2024118709A
JP2024118709A JP2023025141A JP2023025141A JP2024118709A JP 2024118709 A JP2024118709 A JP 2024118709A JP 2023025141 A JP2023025141 A JP 2023025141A JP 2023025141 A JP2023025141 A JP 2023025141A JP 2024118709 A JP2024118709 A JP 2024118709A
Authority
JP
Japan
Prior art keywords
amino acid
seq
lipase
numbering
acid residue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023025141A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024118709A5 (enExample
Inventor
貴大 日置
Takahiro Hioki
彰人 川原
Akihito Kawahara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kao Corp
Original Assignee
Kao Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kao Corp filed Critical Kao Corp
Priority to JP2023025141A priority Critical patent/JP2024118709A/ja
Priority to PCT/JP2024/005942 priority patent/WO2024177043A1/ja
Priority to EP24760341.8A priority patent/EP4671374A1/en
Publication of JP2024118709A publication Critical patent/JP2024118709A/ja
Publication of JP2024118709A5 publication Critical patent/JP2024118709A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
JP2023025141A 2023-02-21 2023-02-21 リパーゼ変異体 Pending JP2024118709A (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2023025141A JP2024118709A (ja) 2023-02-21 2023-02-21 リパーゼ変異体
PCT/JP2024/005942 WO2024177043A1 (ja) 2023-02-21 2024-02-20 リパーゼ変異体
EP24760341.8A EP4671374A1 (en) 2023-02-21 2024-02-20 LIPASE VARIANT

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2023025141A JP2024118709A (ja) 2023-02-21 2023-02-21 リパーゼ変異体

Publications (2)

Publication Number Publication Date
JP2024118709A true JP2024118709A (ja) 2024-09-02
JP2024118709A5 JP2024118709A5 (enExample) 2025-12-25

Family

ID=92501309

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023025141A Pending JP2024118709A (ja) 2023-02-21 2023-02-21 リパーゼ変異体

Country Status (3)

Country Link
EP (1) EP4671374A1 (enExample)
JP (1) JP2024118709A (enExample)
WO (1) WO2024177043A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025126926A1 (ja) * 2023-12-15 2025-06-19 花王株式会社 リパーゼ変異体

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2635288B2 (ja) 1993-03-11 1997-07-30 花王株式会社 漂白剤組成物及び漂白洗浄剤組成物
JP3512981B2 (ja) 1997-05-19 2004-03-31 花王株式会社 耐熱性アルカリセルラ−ゼ、それを生産する微生物及びその製造方法
JP3492935B2 (ja) 1999-04-02 2004-02-03 花王株式会社 プラスミドベクター
JP3872373B2 (ja) 2002-04-18 2007-01-24 花王株式会社 粉末洗浄剤組成物
JP4485341B2 (ja) 2004-12-20 2010-06-23 花王株式会社 組換え微生物
EP2875111A1 (en) 2012-05-16 2015-05-27 Novozymes A/S Compositions comprising lipase and methods of use thereof
US10246692B2 (en) 2012-07-12 2019-04-02 Novozymes A/S Polypeptides having lipase activity and polynucleotides encoding same
CN117448299A (zh) * 2017-09-27 2024-01-26 诺维信公司 脂肪酶变体和包含此类脂肪酶变体的微囊组合物
JP2019182911A (ja) 2018-04-02 2019-10-24 花王株式会社 食器及び／又は台所周りの硬質物品用液体洗浄剤組成物
JP7262333B2 (ja) 2019-07-22 2023-04-21 花王株式会社 硬質物品の洗浄方法
US20230111102A1 (en) 2020-01-27 2023-04-13 Kao Corporation Method for producing the variable domain of the heavy chain of a heavy-chain antibody

Also Published As

Publication number Publication date
EP4671374A1 (en) 2025-12-31
WO2024177043A1 (ja) 2024-08-29

Similar Documents

Publication Publication Date Title
WO2023199945A1 (ja) 新規リパーゼ
WO2024177043A1 (ja) リパーゼ変異体
WO2024177042A1 (ja) リパーゼ変異体
WO2023199946A1 (ja) 新規リパーゼ
WO2022071451A1 (ja) α－アミラーゼ変異体
JP5977029B2 (ja) 変異グリコシドハイドロラーゼ
JP2025096190A (ja) リパーゼの安定性向上方法
WO2025126926A1 (ja) リパーゼ変異体
WO2021064068A1 (en) Polypeptides comprising at least two carbohydrate binding domains
JP7772526B2 (ja) α－アミラーゼ変異体
EP4502161A1 (en) Alpha-amylase variant
JP6081172B2 (ja) 変異グリコシドハイドロラーゼ
JP6144483B2 (ja) 変異グリコシドハイドロラーゼ
JP2023095355A (ja) アミラーゼ配合洗浄剤組成物
JP5714830B2 (ja) 変異アルカリセルラーゼ
JP2024118710A (ja) リパーゼの製造方法
JP5666816B2 (ja) 変異アルカリセルラーゼ
WO2024024527A1 (ja) 変異プロテアーゼ
JP5698460B2 (ja) 変異アルカリセルラーゼ
US20250388887A1 (en) Alpha-amylase variant
JP2023138320A (ja) α－アミラーゼ変異体
WO2023176970A1 (ja) α－アミラーゼ変異体
CN118891362A (zh) α-淀粉酶突变体

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20251216

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20251216