JP2023521505A5 - - Google Patents

Info

Publication number
JP2023521505A5
JP2023521505A5 JP2022563100A JP2022563100A JP2023521505A5 JP 2023521505 A5 JP2023521505 A5 JP 2023521505A5 JP 2022563100 A JP2022563100 A JP 2022563100A JP 2022563100 A JP2022563100 A JP 2022563100A JP 2023521505 A5 JP2023521505 A5 JP 2023521505A5
Authority
JP
Japan
Prior art keywords
stranded rna
solid phase
sample
buffer
eluting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2022563100A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023521505A (ja
JP7783831B2 (ja
Filing date
Publication date
Priority claimed from EP20170205.7A external-priority patent/EP3896161A1/en
Application filed filed Critical
Publication of JP2023521505A publication Critical patent/JP2023521505A/ja
Publication of JP2023521505A5 publication Critical patent/JP2023521505A5/ja
Application granted granted Critical
Publication of JP7783831B2 publication Critical patent/JP7783831B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2022563100A 2020-04-17 2021-04-16 一本鎖rnaの精製方法 Active JP7783831B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170205.7 2020-04-17
EP20170205.7A EP3896161A1 (en) 2020-04-17 2020-04-17 A method of single-stranded rna purification
PCT/EP2021/059903 WO2021209595A2 (en) 2020-04-17 2021-04-16 A method of single-stranded rna purification

Publications (3)

Publication Number Publication Date
JP2023521505A JP2023521505A (ja) 2023-05-24
JP2023521505A5 true JP2023521505A5 (https=) 2025-09-16
JP7783831B2 JP7783831B2 (ja) 2025-12-10

Family

ID=70333789

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022563100A Active JP7783831B2 (ja) 2020-04-17 2021-04-16 一本鎖rnaの精製方法

Country Status (8)

Country Link
US (1) US12227738B2 (https=)
EP (1) EP3896161A1 (https=)
JP (1) JP7783831B2 (https=)
KR (1) KR20230025769A (https=)
CN (1) CN115768888A (https=)
CA (1) CA3177257A1 (https=)
IL (1) IL297116A (https=)
WO (1) WO2021209595A2 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121046364A (zh) * 2022-07-29 2025-12-02 赛诺菲巴斯德有限公司 用于无乙醇mRNA纯化的方法
EP4556572A1 (en) * 2023-11-20 2025-05-21 Sartorius BIA Separations d.o.o. Method for purifying one or more target nucleic acid(s)
WO2025163146A1 (en) 2024-01-31 2025-08-07 Sanofi Methods for ethanol-free mrna purification
WO2025199419A1 (en) * 2024-03-21 2025-09-25 Rensselaer Polytechnic Institute Systems and methods for electrostatic-based purification of nucleic acids
KR20250173969A (ko) * 2024-06-04 2025-12-11 주식회사 녹십자 단일가닥 rna 정제 방법
WO2025257280A1 (en) * 2024-06-14 2025-12-18 Merck Patent Gmbh Method for purification of rna
CN120290548B (zh) * 2025-06-12 2025-09-23 硅羿科技(上海)有限公司 一种dsRNA标准品的纯化和制备方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
DE102004045332B3 (de) * 2004-09-16 2006-03-09 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Isolierung und Trennung von einzel- und doppelsträngigen Nucleinsäuren aus einer diese Nucleinsäuren enthaltenden Probe sowie Trennsäule zur Durchführung dieses Verfahrens
US20060094023A1 (en) * 2004-11-02 2006-05-04 Industrial Technology Research Institute Method for isolating nucleic acid by using amino surfactants
GB2419594C (en) * 2004-11-02 2011-03-02 Ind Tecnology Res Inst Method for stabilizing or isolating nucleic acids.
US20090048439A1 (en) * 2007-08-06 2009-02-19 Weisburg William G Isolation of nucleic acids molecules using modified solid supports
EP2250182B1 (en) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
US10590161B2 (en) 2013-03-15 2020-03-17 Modernatx, Inc. Ion exchange purification of mRNA
BR112016000160B1 (pt) * 2013-07-05 2021-06-08 Bioneer Corporation estrutura de oligonucleotídeo tipo nanopartícula melhorada tendo alta eficiência e método para a preparação da mesma
SI3445850T1 (sl) * 2016-04-22 2021-12-31 BioNTech SE Postopki za zagotavljanje enoverižne RNA
WO2018096179A1 (en) * 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna
EP4215613B1 (en) * 2022-01-24 2026-03-04 Sartorius BIA Separations d.o.o. Method of separating single stranded rna molecules from other nucleic acid species, use of solid phase therefor, and method of separating double stranded rna molecules by size

Similar Documents

Publication Publication Date Title
JP2023521505A5 (https=)
JP2023521504A5 (https=)
US8895707B2 (en) Elution of proteins from hydroxyapatite resins without resin deterioration
JP2007532477A5 (https=)
JP2012018171A5 (https=)
JP7746285B2 (ja) 一本鎖rnaの精製方法
US12227738B2 (en) Method of single-stranded RNA purification
JP2023536085A5 (https=)
CA2797040A1 (en) Simple method for simultaneous removal of multiple impurities from culture supernatants to ultralow levels
CN116261483B (zh) 多模式金属亲和加工衣壳
RU2014111820A (ru) Способы элиминации вируса
CN105517718B (zh) 磷灰石原位恢复
CN106754890B (zh) 一种病毒rna的提取试剂盒和提取方法
CN101363045B (zh) 一种用超微磁颗粒分离纯化生物靶物质的方法
JPWO2023189652A5 (https=)
JP2023522526A5 (https=)
EP3896159B1 (en) A method of single strand rna purification employing an anion exchanger
US8741619B1 (en) Method for purifying protein aqueous
CN116348598B (zh) 通过疏水相互作用色谱法改善dna质粒制剂中rna和污染物的去除
EP2712925B1 (en) Protein removal agent
CN116348598A (zh) 通过疏水相互作用色谱法改善dna质粒制剂中rna和污染物的去除
CN117866038A (zh) 纯化含有宿主核酸的带亲和标签的酸性蛋白的方法
CN121046367A (zh) 一种自动化体液样本病原体与宿主基因组核酸同步提取方法