CN115768888A - 单链rna的纯化方法 - Google Patents

单链rna的纯化方法 Download PDF

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Publication number
CN115768888A
CN115768888A CN202180029140.XA CN202180029140A CN115768888A CN 115768888 A CN115768888 A CN 115768888A CN 202180029140 A CN202180029140 A CN 202180029140A CN 115768888 A CN115768888 A CN 115768888A
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China
Prior art keywords
ssrna
solid phase
stranded rna
buffer
salt
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Pending
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CN202180029140.XA
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English (en)
Chinese (zh)
Inventor
S·佩尔西奇
U·塞尼戈吉
D·多伦茨
P·S·加农
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Sedolis Biya Separation Co ltd
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Sedolis Biya Separation Co ltd
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Publication of CN115768888A publication Critical patent/CN115768888A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CN202180029140.XA 2020-04-17 2021-04-16 单链rna的纯化方法 Pending CN115768888A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170205.7 2020-04-17
EP20170205.7A EP3896161A1 (en) 2020-04-17 2020-04-17 A method of single-stranded rna purification
PCT/EP2021/059903 WO2021209595A2 (en) 2020-04-17 2021-04-16 A method of single-stranded rna purification

Publications (1)

Publication Number Publication Date
CN115768888A true CN115768888A (zh) 2023-03-07

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ID=70333789

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180029140.XA Pending CN115768888A (zh) 2020-04-17 2021-04-16 单链rna的纯化方法

Country Status (8)

Country Link
US (1) US12227738B2 (https=)
EP (1) EP3896161A1 (https=)
JP (1) JP7783831B2 (https=)
KR (1) KR20230025769A (https=)
CN (1) CN115768888A (https=)
CA (1) CA3177257A1 (https=)
IL (1) IL297116A (https=)
WO (1) WO2021209595A2 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121046364A (zh) * 2022-07-29 2025-12-02 赛诺菲巴斯德有限公司 用于无乙醇mRNA纯化的方法
EP4556572A1 (en) * 2023-11-20 2025-05-21 Sartorius BIA Separations d.o.o. Method for purifying one or more target nucleic acid(s)
WO2025163146A1 (en) 2024-01-31 2025-08-07 Sanofi Methods for ethanol-free mrna purification
WO2025199419A1 (en) * 2024-03-21 2025-09-25 Rensselaer Polytechnic Institute Systems and methods for electrostatic-based purification of nucleic acids
KR20250173969A (ko) * 2024-06-04 2025-12-11 주식회사 녹십자 단일가닥 rna 정제 방법
WO2025257280A1 (en) * 2024-06-14 2025-12-18 Merck Patent Gmbh Method for purification of rna
CN120290548B (zh) * 2025-06-12 2025-09-23 硅羿科技（上海）有限公司 一种dsRNA标准品的纯化和制备方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0270017A2 (en) * 1986-12-01 1988-06-08 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
GB0512937D0 (en) * 2004-11-02 2005-08-03 Ind Tech Res Inst Method for stabilizing or isolating nucleic acids
US20060094023A1 (en) * 2004-11-02 2006-05-04 Industrial Technology Research Institute Method for isolating nucleic acid by using amino surfactants
CN118715319A (zh) * 2022-01-24 2024-09-27 赛多利斯比亚分离有限责任公司 将单链rna分子与其他核酸种类分离的方法、用于其的固相的用途以及依尺寸分离双链rna分子的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004045332B3 (de) * 2004-09-16 2006-03-09 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Isolierung und Trennung von einzel- und doppelsträngigen Nucleinsäuren aus einer diese Nucleinsäuren enthaltenden Probe sowie Trennsäule zur Durchführung dieses Verfahrens
US20090048439A1 (en) * 2007-08-06 2009-02-19 Weisburg William G Isolation of nucleic acids molecules using modified solid supports
EP2250182B1 (en) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
US10590161B2 (en) 2013-03-15 2020-03-17 Modernatx, Inc. Ion exchange purification of mRNA
BR112016000160B1 (pt) * 2013-07-05 2021-06-08 Bioneer Corporation estrutura de oligonucleotídeo tipo nanopartícula melhorada tendo alta eficiência e método para a preparação da mesma
SI3445850T1 (sl) * 2016-04-22 2021-12-31 BioNTech SE Postopki za zagotavljanje enoverižne RNA
WO2018096179A1 (en) * 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0270017A2 (en) * 1986-12-01 1988-06-08 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
GB0512937D0 (en) * 2004-11-02 2005-08-03 Ind Tech Res Inst Method for stabilizing or isolating nucleic acids
US20060094023A1 (en) * 2004-11-02 2006-05-04 Industrial Technology Research Institute Method for isolating nucleic acid by using amino surfactants
CN118715319A (zh) * 2022-01-24 2024-09-27 赛多利斯比亚分离有限责任公司 将单链rna分子与其他核酸种类分离的方法、用于其的固相的用途以及依尺寸分离双链rna分子的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ROK MIKLAVˇCIˇC等: "High Recovery Chromatographic Purification of mRNA at Room Temperature and Neutral pH", 《INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES》, 19 September 2023 (2023-09-19), pages 1 - 17 *
SARTORIOUS: "One-Step Purification of Research- Grade Single-Stranded mRNA", pages 1 - 2, Retrieved from the Internet <URL:https://www.biaseparations.com/librabary_items/One-Step Purification of Research- Grade Single-Stranded mRNA/> *

Also Published As

Publication number Publication date
IL297116A (en) 2022-12-01
US12227738B2 (en) 2025-02-18
WO2021209595A2 (en) 2021-10-21
EP3896161A1 (en) 2021-10-20
JP2023521505A (ja) 2023-05-24
WO2021209595A3 (en) 2021-11-25
US20230151351A1 (en) 2023-05-18
CA3177257A1 (en) 2021-10-21
JP7783831B2 (ja) 2025-12-10
KR20230025769A (ko) 2023-02-23

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Inventor after: S. Persychid Benzac

Inventor after: U. Senigogi

Inventor after: D. Dorenz

Inventor after: GAGNON PETER S

Inventor before: S. Persich

Inventor before: U. Senigogi

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