CA3177257A1 - A method of single-stranded rna purification - Google Patents

A method of single-stranded rna purification

Info

Publication number
CA3177257A1
CA3177257A1 CA3177257A CA3177257A CA3177257A1 CA 3177257 A1 CA3177257 A1 CA 3177257A1 CA 3177257 A CA3177257 A CA 3177257A CA 3177257 A CA3177257 A CA 3177257A CA 3177257 A1 CA3177257 A1 CA 3177257A1
Authority
CA
Canada
Prior art keywords
stranded rna
solid phase
rna purification
purification
dominantly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3177257A
Other languages
English (en)
French (fr)
Inventor
Spela Persic Debenjak
Urh Cernigoj
Darko DOLENC
Peter Stanley GAGNON
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sartorius Bia Separations doo
Original Assignee
Sartorius Bia Separations doo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sartorius Bia Separations doo filed Critical Sartorius Bia Separations doo
Publication of CA3177257A1 publication Critical patent/CA3177257A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CA3177257A 2020-04-17 2021-04-16 A method of single-stranded rna purification Pending CA3177257A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170205.7 2020-04-17
EP20170205.7A EP3896161A1 (en) 2020-04-17 2020-04-17 A method of single-stranded rna purification
PCT/EP2021/059903 WO2021209595A2 (en) 2020-04-17 2021-04-16 A method of single-stranded rna purification

Publications (1)

Publication Number Publication Date
CA3177257A1 true CA3177257A1 (en) 2021-10-21

Family

ID=70333789

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3177257A Pending CA3177257A1 (en) 2020-04-17 2021-04-16 A method of single-stranded rna purification

Country Status (8)

Country Link
US (1) US12227738B2 (https=)
EP (1) EP3896161A1 (https=)
JP (1) JP7783831B2 (https=)
KR (1) KR20230025769A (https=)
CN (1) CN115768888A (https=)
CA (1) CA3177257A1 (https=)
IL (1) IL297116A (https=)
WO (1) WO2021209595A2 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121046364A (zh) * 2022-07-29 2025-12-02 赛诺菲巴斯德有限公司 用于无乙醇mRNA纯化的方法
EP4556572A1 (en) * 2023-11-20 2025-05-21 Sartorius BIA Separations d.o.o. Method for purifying one or more target nucleic acid(s)
WO2025163146A1 (en) 2024-01-31 2025-08-07 Sanofi Methods for ethanol-free mrna purification
WO2025199419A1 (en) * 2024-03-21 2025-09-25 Rensselaer Polytechnic Institute Systems and methods for electrostatic-based purification of nucleic acids
KR20250173969A (ko) * 2024-06-04 2025-12-11 주식회사 녹십자 단일가닥 rna 정제 방법
WO2025257280A1 (en) * 2024-06-14 2025-12-18 Merck Patent Gmbh Method for purification of rna
CN120290548B (zh) * 2025-06-12 2025-09-23 硅羿科技（上海）有限公司 一种dsRNA标准品的纯化和制备方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
DE102004045332B3 (de) * 2004-09-16 2006-03-09 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Isolierung und Trennung von einzel- und doppelsträngigen Nucleinsäuren aus einer diese Nucleinsäuren enthaltenden Probe sowie Trennsäule zur Durchführung dieses Verfahrens
US20060094023A1 (en) * 2004-11-02 2006-05-04 Industrial Technology Research Institute Method for isolating nucleic acid by using amino surfactants
GB2419594C (en) * 2004-11-02 2011-03-02 Ind Tecnology Res Inst Method for stabilizing or isolating nucleic acids.
US20090048439A1 (en) * 2007-08-06 2009-02-19 Weisburg William G Isolation of nucleic acids molecules using modified solid supports
EP2250182B1 (en) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
US10590161B2 (en) 2013-03-15 2020-03-17 Modernatx, Inc. Ion exchange purification of mRNA
BR112016000160B1 (pt) * 2013-07-05 2021-06-08 Bioneer Corporation estrutura de oligonucleotídeo tipo nanopartícula melhorada tendo alta eficiência e método para a preparação da mesma
SI3445850T1 (sl) * 2016-04-22 2021-12-31 BioNTech SE Postopki za zagotavljanje enoverižne RNA
WO2018096179A1 (en) * 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna
EP4215613B1 (en) * 2022-01-24 2026-03-04 Sartorius BIA Separations d.o.o. Method of separating single stranded rna molecules from other nucleic acid species, use of solid phase therefor, and method of separating double stranded rna molecules by size

Also Published As

Publication number Publication date
IL297116A (en) 2022-12-01
US12227738B2 (en) 2025-02-18
WO2021209595A2 (en) 2021-10-21
EP3896161A1 (en) 2021-10-20
JP2023521505A (ja) 2023-05-24
WO2021209595A3 (en) 2021-11-25
US20230151351A1 (en) 2023-05-18
CN115768888A (zh) 2023-03-07
JP7783831B2 (ja) 2025-12-10
KR20230025769A (ko) 2023-02-23

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