KR20230025769A - 단일 가닥 rna 정제 방법 - Google Patents

단일 가닥 rna 정제 방법 Download PDF

Info

Publication number
KR20230025769A
KR20230025769A KR1020227035727A KR20227035727A KR20230025769A KR 20230025769 A KR20230025769 A KR 20230025769A KR 1020227035727 A KR1020227035727 A KR 1020227035727A KR 20227035727 A KR20227035727 A KR 20227035727A KR 20230025769 A KR20230025769 A KR 20230025769A
Authority
KR
South Korea
Prior art keywords
solid phase
ssrna
stranded rna
buffer
gradient
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020227035727A
Other languages
English (en)
Korean (ko)
Inventor
스펠라 페르시치
우르 체르니고이
다르코 돌렌
피터 스탠리 가뇽
Original Assignee
사르토리우스 비아이에이 세퍼레이션스 디.오.오.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 사르토리우스 비아이에이 세퍼레이션스 디.오.오. filed Critical 사르토리우스 비아이에이 세퍼레이션스 디.오.오.
Publication of KR20230025769A publication Critical patent/KR20230025769A/ko
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
KR1020227035727A 2020-04-17 2021-04-16 단일 가닥 rna 정제 방법 Pending KR20230025769A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170205.7 2020-04-17
EP20170205.7A EP3896161A1 (en) 2020-04-17 2020-04-17 A method of single-stranded rna purification
PCT/EP2021/059903 WO2021209595A2 (en) 2020-04-17 2021-04-16 A method of single-stranded rna purification

Publications (1)

Publication Number Publication Date
KR20230025769A true KR20230025769A (ko) 2023-02-23

Family

ID=70333789

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020227035727A Pending KR20230025769A (ko) 2020-04-17 2021-04-16 단일 가닥 rna 정제 방법

Country Status (8)

Country Link
US (1) US12227738B2 (https=)
EP (1) EP3896161A1 (https=)
JP (1) JP7783831B2 (https=)
KR (1) KR20230025769A (https=)
CN (1) CN115768888A (https=)
CA (1) CA3177257A1 (https=)
IL (1) IL297116A (https=)
WO (1) WO2021209595A2 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121046364A (zh) * 2022-07-29 2025-12-02 赛诺菲巴斯德有限公司 用于无乙醇mRNA纯化的方法
EP4556572A1 (en) * 2023-11-20 2025-05-21 Sartorius BIA Separations d.o.o. Method for purifying one or more target nucleic acid(s)
WO2025163146A1 (en) 2024-01-31 2025-08-07 Sanofi Methods for ethanol-free mrna purification
WO2025199419A1 (en) * 2024-03-21 2025-09-25 Rensselaer Polytechnic Institute Systems and methods for electrostatic-based purification of nucleic acids
KR20250173969A (ko) * 2024-06-04 2025-12-11 주식회사 녹십자 단일가닥 rna 정제 방법
WO2025257280A1 (en) * 2024-06-14 2025-12-18 Merck Patent Gmbh Method for purification of rna
CN120290548B (zh) * 2025-06-12 2025-09-23 硅羿科技（上海）有限公司 一种dsRNA标准品的纯化和制备方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
DE102004045332B3 (de) * 2004-09-16 2006-03-09 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Isolierung und Trennung von einzel- und doppelsträngigen Nucleinsäuren aus einer diese Nucleinsäuren enthaltenden Probe sowie Trennsäule zur Durchführung dieses Verfahrens
US20060094023A1 (en) * 2004-11-02 2006-05-04 Industrial Technology Research Institute Method for isolating nucleic acid by using amino surfactants
GB2419594C (en) * 2004-11-02 2011-03-02 Ind Tecnology Res Inst Method for stabilizing or isolating nucleic acids.
US20090048439A1 (en) * 2007-08-06 2009-02-19 Weisburg William G Isolation of nucleic acids molecules using modified solid supports
EP2250182B1 (en) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
US10590161B2 (en) 2013-03-15 2020-03-17 Modernatx, Inc. Ion exchange purification of mRNA
BR112016000160B1 (pt) * 2013-07-05 2021-06-08 Bioneer Corporation estrutura de oligonucleotídeo tipo nanopartícula melhorada tendo alta eficiência e método para a preparação da mesma
SI3445850T1 (sl) * 2016-04-22 2021-12-31 BioNTech SE Postopki za zagotavljanje enoverižne RNA
WO2018096179A1 (en) * 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna
EP4215613B1 (en) * 2022-01-24 2026-03-04 Sartorius BIA Separations d.o.o. Method of separating single stranded rna molecules from other nucleic acid species, use of solid phase therefor, and method of separating double stranded rna molecules by size

Also Published As

Publication number Publication date
IL297116A (en) 2022-12-01
US12227738B2 (en) 2025-02-18
WO2021209595A2 (en) 2021-10-21
EP3896161A1 (en) 2021-10-20
JP2023521505A (ja) 2023-05-24
WO2021209595A3 (en) 2021-11-25
US20230151351A1 (en) 2023-05-18
CA3177257A1 (en) 2021-10-21
CN115768888A (zh) 2023-03-07
JP7783831B2 (ja) 2025-12-10

Similar Documents

Publication Publication Date Title
KR20230025769A (ko) 단일 가닥 rna 정제 방법
US12522816B2 (en) Method of single-stranded RNA purification
KR102919038B1 (ko) 음이온 교환 크로마토그래피를 이용한 플라스미드 dna와 같은 생물학적 분자의 정제 공정
CN116261483B (zh) 多模式金属亲和加工衣壳
WO2020127505A1 (en) A method for removing nucleosomal contaminants from bioprocessing solutions
WO2001037987A1 (en) A method for selective removal of a substance from samples containing compounds having nucleic acid structure
EP3896159B1 (en) A method of single strand rna purification employing an anion exchanger
US20090047734A1 (en) Method of separation of deoxyribonucleic acids
JP2024528261A (ja) 核酸精製におけるエンドトキシンレベルを低減するための方法
EP4732926A1 (en) Multivalent halide salts for use in hydrophobic interaction chromatography
US20110152510A1 (en) Simple load and elute process for purification of genomic dna
JP2025506274A (ja) 生物学的薬剤の精製プロセス中の夾雑物の選択的除去方法
EP4556572A1 (en) Method for purifying one or more target nucleic acid(s)
US20100036109A1 (en) Method for selectively and reversibly adsorbing nucleic acids on a carrier material
WO2026093528A1 (en) A purification of rna by flowthrough chromatography
KR20240159599A (ko) 핵산 정제에서 엔도톡신 수준을 감소시키는 방법
HK40058215A (zh) 采用阴离子交换色谱法的生物分子(如质粒dna)纯化工艺

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R13-asn-PN2301

St.27 status event code: A-3-3-R10-R11-asn-PN2301

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

R15-X000 Change to inventor requested

St.27 status event code: A-3-3-R10-R15-oth-X000

R16-X000 Change to inventor recorded

St.27 status event code: A-3-3-R10-R16-oth-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

D21 Rejection of application intended

Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D21-EXM-PE0902 (AS PROVIDED BY THE NATIONAL OFFICE)

PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902