IL297116A - A method for purifying single-stranded RNA - Google Patents
A method for purifying single-stranded RNAInfo
- Publication number
- IL297116A IL297116A IL297116A IL29711622A IL297116A IL 297116 A IL297116 A IL 297116A IL 297116 A IL297116 A IL 297116A IL 29711622 A IL29711622 A IL 29711622A IL 297116 A IL297116 A IL 297116A
- Authority
- IL
- Israel
- Prior art keywords
- ssrna
- solid phase
- stranded rna
- buffer
- salt
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20170205.7A EP3896161A1 (en) | 2020-04-17 | 2020-04-17 | A method of single-stranded rna purification |
| PCT/EP2021/059903 WO2021209595A2 (en) | 2020-04-17 | 2021-04-16 | A method of single-stranded rna purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL297116A true IL297116A (en) | 2022-12-01 |
Family
ID=70333789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL297116A IL297116A (en) | 2020-04-17 | 2021-04-16 | A method for purifying single-stranded RNA |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US12227738B2 (https=) |
| EP (1) | EP3896161A1 (https=) |
| JP (1) | JP7783831B2 (https=) |
| KR (1) | KR20230025769A (https=) |
| CN (1) | CN115768888A (https=) |
| CA (1) | CA3177257A1 (https=) |
| IL (1) | IL297116A (https=) |
| WO (1) | WO2021209595A2 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN121046364A (zh) * | 2022-07-29 | 2025-12-02 | 赛诺菲巴斯德有限公司 | 用于无乙醇mRNA纯化的方法 |
| EP4556572A1 (en) * | 2023-11-20 | 2025-05-21 | Sartorius BIA Separations d.o.o. | Method for purifying one or more target nucleic acid(s) |
| WO2025163146A1 (en) | 2024-01-31 | 2025-08-07 | Sanofi | Methods for ethanol-free mrna purification |
| WO2025199419A1 (en) * | 2024-03-21 | 2025-09-25 | Rensselaer Polytechnic Institute | Systems and methods for electrostatic-based purification of nucleic acids |
| KR20250173969A (ko) * | 2024-06-04 | 2025-12-11 | 주식회사 녹십자 | 단일가닥 rna 정제 방법 |
| WO2025257280A1 (en) * | 2024-06-14 | 2025-12-18 | Merck Patent Gmbh | Method for purification of rna |
| CN120290548B (zh) * | 2025-06-12 | 2025-09-23 | 硅羿科技（上海）有限公司 | 一种dsRNA标准品的纯化和制备方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4935342A (en) * | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| DE102004045332B3 (de) * | 2004-09-16 | 2006-03-09 | Macherey, Nagel Gmbh & Co. Handelsgesellschaft | Verfahren zur Isolierung und Trennung von einzel- und doppelsträngigen Nucleinsäuren aus einer diese Nucleinsäuren enthaltenden Probe sowie Trennsäule zur Durchführung dieses Verfahrens |
| US20060094023A1 (en) * | 2004-11-02 | 2006-05-04 | Industrial Technology Research Institute | Method for isolating nucleic acid by using amino surfactants |
| GB2419594C (en) * | 2004-11-02 | 2011-03-02 | Ind Tecnology Res Inst | Method for stabilizing or isolating nucleic acids. |
| US20090048439A1 (en) * | 2007-08-06 | 2009-02-19 | Weisburg William G | Isolation of nucleic acids molecules using modified solid supports |
| EP2250182B1 (en) * | 2008-02-07 | 2015-12-02 | GE Healthcare Bio-Sciences Corp. | Isolation of dna, rna and protein from a single sample |
| US10590161B2 (en) | 2013-03-15 | 2020-03-17 | Modernatx, Inc. | Ion exchange purification of mRNA |
| BR112016000160B1 (pt) * | 2013-07-05 | 2021-06-08 | Bioneer Corporation | estrutura de oligonucleotídeo tipo nanopartícula melhorada tendo alta eficiência e método para a preparação da mesma |
| SI3445850T1 (sl) * | 2016-04-22 | 2021-12-31 | BioNTech SE | Postopki za zagotavljanje enoverižne RNA |
| WO2018096179A1 (en) * | 2016-11-28 | 2018-05-31 | Curevac Ag | Method for purifying rna |
| EP4215613B1 (en) * | 2022-01-24 | 2026-03-04 | Sartorius BIA Separations d.o.o. | Method of separating single stranded rna molecules from other nucleic acid species, use of solid phase therefor, and method of separating double stranded rna molecules by size |
-
2020
- 2020-04-17 EP EP20170205.7A patent/EP3896161A1/en active Pending
-
2021
- 2021-04-16 KR KR1020227035727A patent/KR20230025769A/ko active Pending
- 2021-04-16 CA CA3177257A patent/CA3177257A1/en active Pending
- 2021-04-16 CN CN202180029140.XA patent/CN115768888A/zh active Pending
- 2021-04-16 IL IL297116A patent/IL297116A/en unknown
- 2021-04-16 US US17/917,084 patent/US12227738B2/en active Active
- 2021-04-16 JP JP2022563100A patent/JP7783831B2/ja active Active
- 2021-04-16 WO PCT/EP2021/059903 patent/WO2021209595A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| US12227738B2 (en) | 2025-02-18 |
| WO2021209595A2 (en) | 2021-10-21 |
| EP3896161A1 (en) | 2021-10-20 |
| JP2023521505A (ja) | 2023-05-24 |
| WO2021209595A3 (en) | 2021-11-25 |
| US20230151351A1 (en) | 2023-05-18 |
| CA3177257A1 (en) | 2021-10-21 |
| CN115768888A (zh) | 2023-03-07 |
| JP7783831B2 (ja) | 2025-12-10 |
| KR20230025769A (ko) | 2023-02-23 |
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