JP7783831B2 - 一本鎖ｒｎａの精製方法 - Google Patents

一本鎖ｒｎａの精製方法

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Publication number
JP7783831B2
JP7783831B2 JP2022563100A JP2022563100A JP7783831B2 JP 7783831 B2 JP7783831 B2 JP 7783831B2 JP 2022563100 A JP2022563100 A JP 2022563100A JP 2022563100 A JP2022563100 A JP 2022563100A JP 7783831 B2 JP7783831 B2 JP 7783831B2
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Japan
Prior art keywords
solid phase
ssrna
stranded rna
buffer
sample
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JP2022563100A
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English (en)
Japanese (ja)
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JP2023521505A5 (https=
JP2023521505A (ja
Inventor
ペルシッチ、スペラ
チェルニゴイ、ウルフ
ドレンツ、ダルコ
スタンリー ギャニオン、ピーター
Original Assignee
ザルトリウス ビーアイエー セパレーションズ ディー．オー．オー．
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Publication of JP2023521505A publication Critical patent/JP2023521505A/ja
Publication of JP2023521505A5 publication Critical patent/JP2023521505A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
JP2022563100A 2020-04-17 2021-04-16 一本鎖ｒｎａの精製方法 Active JP7783831B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170205.7 2020-04-17
EP20170205.7A EP3896161A1 (en) 2020-04-17 2020-04-17 A method of single-stranded rna purification
PCT/EP2021/059903 WO2021209595A2 (en) 2020-04-17 2021-04-16 A method of single-stranded rna purification

Publications (3)

Publication Number Publication Date
JP2023521505A JP2023521505A (ja) 2023-05-24
JP2023521505A5 JP2023521505A5 (https=) 2025-09-16
JP7783831B2 true JP7783831B2 (ja) 2025-12-10

Family

ID=70333789

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022563100A Active JP7783831B2 (ja) 2020-04-17 2021-04-16 一本鎖ｒｎａの精製方法

Country Status (8)

Country Link
US (1) US12227738B2 (https=)
EP (1) EP3896161A1 (https=)
JP (1) JP7783831B2 (https=)
KR (1) KR20230025769A (https=)
CN (1) CN115768888A (https=)
CA (1) CA3177257A1 (https=)
IL (1) IL297116A (https=)
WO (1) WO2021209595A2 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121046364A (zh) * 2022-07-29 2025-12-02 赛诺菲巴斯德有限公司 用于无乙醇mRNA纯化的方法
EP4556572A1 (en) * 2023-11-20 2025-05-21 Sartorius BIA Separations d.o.o. Method for purifying one or more target nucleic acid(s)
WO2025163146A1 (en) 2024-01-31 2025-08-07 Sanofi Methods for ethanol-free mrna purification
WO2025199419A1 (en) * 2024-03-21 2025-09-25 Rensselaer Polytechnic Institute Systems and methods for electrostatic-based purification of nucleic acids
KR20250173969A (ko) * 2024-06-04 2025-12-11 주식회사 녹십자 단일가닥 rna 정제 방법
WO2025257280A1 (en) * 2024-06-14 2025-12-18 Merck Patent Gmbh Method for purification of rna
CN120290548B (zh) * 2025-06-12 2025-09-23 硅羿科技（上海）有限公司 一种dsRNA标准品的纯化和制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009100172A1 (en) 2008-02-07 2009-08-13 Ge Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
JP2016530875A (ja) 2013-07-05 2016-10-06 バイオニア コーポレーションＢｉｏｎｅｅｒ Ｃｏｒｐｏｒａｔｉｏｎ 改善された高効率ナノ粒子型オリゴヌクレオチド構造体およびその製造方法
WO2017182524A1 (en) 2016-04-22 2017-10-26 Biontech Rna Pharmaceuticals Gmbh Methods for providing single-stranded rna
WO2018096179A1 (en) 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
DE102004045332B3 (de) * 2004-09-16 2006-03-09 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Isolierung und Trennung von einzel- und doppelsträngigen Nucleinsäuren aus einer diese Nucleinsäuren enthaltenden Probe sowie Trennsäule zur Durchführung dieses Verfahrens
US20060094023A1 (en) * 2004-11-02 2006-05-04 Industrial Technology Research Institute Method for isolating nucleic acid by using amino surfactants
GB2419594C (en) * 2004-11-02 2011-03-02 Ind Tecnology Res Inst Method for stabilizing or isolating nucleic acids.
US20090048439A1 (en) * 2007-08-06 2009-02-19 Weisburg William G Isolation of nucleic acids molecules using modified solid supports
EP4215613B1 (en) * 2022-01-24 2026-03-04 Sartorius BIA Separations d.o.o. Method of separating single stranded rna molecules from other nucleic acid species, use of solid phase therefor, and method of separating double stranded rna molecules by size

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009100172A1 (en) 2008-02-07 2009-08-13 Ge Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
JP2016530875A (ja) 2013-07-05 2016-10-06 バイオニア コーポレーションＢｉｏｎｅｅｒ Ｃｏｒｐｏｒａｔｉｏｎ 改善された高効率ナノ粒子型オリゴヌクレオチド構造体およびその製造方法
WO2017182524A1 (en) 2016-04-22 2017-10-26 Biontech Rna Pharmaceuticals Gmbh Methods for providing single-stranded rna
WO2018096179A1 (en) 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Chemistry of Natural Compounds，1992年，Vol.28，p.127-128
Journal of Chromatography A，2013年，Vol.1278，p.54-60
Microbes Environ.，2015年，Vol.30, No.2，p.199-203
Molecular Therapy: Nucleic Acids，2019年，Vol.15，p.26-35

Also Published As

Publication number Publication date
IL297116A (en) 2022-12-01
US12227738B2 (en) 2025-02-18
WO2021209595A2 (en) 2021-10-21
EP3896161A1 (en) 2021-10-20
JP2023521505A (ja) 2023-05-24
WO2021209595A3 (en) 2021-11-25
US20230151351A1 (en) 2023-05-18
CA3177257A1 (en) 2021-10-21
CN115768888A (zh) 2023-03-07
KR20230025769A (ko) 2023-02-23

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