JP2022519935A - Ｃａｒ－ｎｋ細胞の作製方法およびその使用方法 - Google Patents

Ｃａｒ－ｎｋ細胞の作製方法およびその使用方法 Download PDF

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JP2022519935A
JP2022519935A JP2021557909A JP2021557909A JP2022519935A JP 2022519935 A JP2022519935 A JP 2022519935A JP 2021557909 A JP2021557909 A JP 2021557909A JP 2021557909 A JP2021557909 A JP 2021557909A JP 2022519935 A JP2022519935 A JP 2022519935A
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cells
car
cell
antigen
cancer
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JP2022519935A5 (enExample
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レズヴァニ、ケイティ
シュポール、エリザベス
リウ、エンリー
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University of Texas System
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  • Medicinal Preparation (AREA)
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JP2021557909A 2019-03-29 2020-03-25 Ｃａｒ－ｎｋ細胞の作製方法およびその使用方法 Pending JP2022519935A (ja)

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Application Number Priority Date Filing Date Title
US201962826856P 2019-03-29 2019-03-29
US62/826,856 2019-03-29
PCT/US2020/024671 WO2020205359A1 (en) 2019-03-29 2020-03-25 Methods for production of car-nk cells and use thereof

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JP2022519935A true JP2022519935A (ja) 2022-03-25
JP2022519935A5 JP2022519935A5 (enExample) 2023-04-03

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US (1) US20220325245A1 (enExample)
EP (1) EP3947647A4 (enExample)
JP (1) JP2022519935A (enExample)
KR (1) KR20220004992A (enExample)
CN (1) CN113811604A (enExample)
AR (1) AR118510A1 (enExample)
AU (1) AU2020254420A1 (enExample)
BR (1) BR112021019411A2 (enExample)
CA (1) CA3135407A1 (enExample)
CO (1) CO2021012719A2 (enExample)
MX (1) MX2021011816A (enExample)
TW (1) TW202104252A (enExample)
WO (1) WO2020205359A1 (enExample)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL276365B2 (en) 2018-02-01 2023-10-01 Nkmax Co Ltd A method for the production of natural killer cells and a preparation for cancer treatment
US12473336B2 (en) 2018-02-21 2025-11-18 Board Of Regents, The University Of Texas System Methods for activation and expansion of natural killer cells and uses thereof
KR20210005240A (ko) * 2018-05-03 2021-01-13 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 면역 관문 차단과 함께 키메라 항원 수용체를 발현하도록 가공된 천연 킬러 세포
GB202005216D0 (en) * 2020-04-08 2020-05-20 Autolus Ltd Cell
US20240325443A1 (en) * 2021-07-23 2024-10-03 Board Of Regents, The University Of Texas System Cd3-expressing natural killer cells with enhanced function for adoptive immunotherapy
CN116262793A (zh) * 2021-12-13 2023-06-16 上海恒润达生生物科技股份有限公司 膜整合型融合蛋白、含有其的细胞及用途
CA3244259A1 (en) * 2022-02-23 2023-08-31 Aloe Therapeutics Inc Compositions and methods for modulating the immune system
CN115896016B (zh) * 2022-09-07 2023-09-12 普华赛尔生物医疗科技有限公司 培养组合物及其在培养免疫细胞中的应用
WO2024058589A1 (ko) * 2022-09-16 2024-03-21 한국과학기술연구원 유전자 가위 넉-인을 이용하여 제조한 키메릭 항원 수용체 세포 및 이의 용도
IL320156A (en) * 2022-10-10 2025-06-01 Kite Pharma Inc New large scale car-t immune cell manufacturing method utilizing lentiviral vector transfection
CN116168765B (zh) * 2023-04-25 2023-08-18 山东大学 基于改进strobemer的基因序列生成方法及系统
AU2024279278A1 (en) 2023-05-31 2025-12-18 Capstan Therapeutics, Inc. Lipid nanoparticle formulations and compositions
WO2025006799A1 (en) * 2023-06-27 2025-01-02 Capstan Therapeutics, Inc. Extracorporeal and ex vivo engineering of select cell populations from peripheral blood
CN116769722B (zh) * 2023-07-04 2024-07-16 杭州荣谷生物科技有限公司 功能增强型car-nk细胞、其制备方法及其在免疫疗法中的应用
CN121889169A (zh) * 2023-09-25 2026-04-17 南京传奇生物科技有限公司 经修饰的免疫细胞和钙调磷酸酶抑制剂及其用途
WO2025169136A1 (en) * 2024-02-09 2025-08-14 Takeda Pharmaceutical Company Limited Dosing regimen for cd19 car nk cells in treating cancer
US20250333700A1 (en) * 2024-02-28 2025-10-30 Gene Solutions Joint Stock Company Modified k562 feeder cell line expressing factors that enhance the activation and proliferation of natural killer cells and a method for its production

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017532353A (ja) * 2014-10-27 2017-11-02 ユニバーシティ オブ セントラル フロリダ リサーチ ファウンデーション，インコーポレイテッド ナチュラルキラー細胞のための方法及び組成物
WO2018195339A1 (en) * 2017-04-19 2018-10-25 Board Of Regents, The University Of Texas System Immune cells expressing engineered antigen receptors
JP2021513860A (ja) * 2018-02-21 2021-06-03 ボード オブ リージェンツ, ザ ユニバーシティ オブ テキサス システムBoard Of Regents, The University Of Texas System ユニバーサル抗原提示細胞およびその使用
JP2021522798A (ja) * 2018-05-03 2021-09-02 ボード オブ リージェンツ, ザ ユニバーシティ オブ テキサス システムBoard Of Regents, The University Of Texas System 免疫チェックポイント遮断によりキメラ抗原受容体を発現するように操作されたナチュラルキラー細胞

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3138905A1 (en) * 2015-09-04 2017-03-08 Miltenyi Biotec GmbH Method for natural killer cell expansion
CN107868791B (zh) * 2016-09-26 2021-11-23 阿思科力(苏州)生物科技有限公司 一种加强型Slit2 CAR-T和CAR-NK细胞制备方法和应用
EP3790958A1 (en) * 2018-05-08 2021-03-17 Life Technologies Corporation Compositions and methods for culturing and expanding cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017532353A (ja) * 2014-10-27 2017-11-02 ユニバーシティ オブ セントラル フロリダ リサーチ ファウンデーション，インコーポレイテッド ナチュラルキラー細胞のための方法及び組成物
WO2018195339A1 (en) * 2017-04-19 2018-10-25 Board Of Regents, The University Of Texas System Immune cells expressing engineered antigen receptors
JP2021513860A (ja) * 2018-02-21 2021-06-03 ボード オブ リージェンツ, ザ ユニバーシティ オブ テキサス システムBoard Of Regents, The University Of Texas System ユニバーサル抗原提示細胞およびその使用
JP2021522798A (ja) * 2018-05-03 2021-09-02 ボード オブ リージェンツ, ザ ユニバーシティ オブ テキサス システムBoard Of Regents, The University Of Texas System 免疫チェックポイント遮断によりキメラ抗原受容体を発現するように操作されたナチュラルキラー細胞

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CECELE J. DENMAN ET AL., PLOS ONE, vol. Vol. 7, Issue 1, Article e30264, JPN6024016704, 2012, ISSN: 0005316792 *
ENLI LIU ET AL., BLOOD, vol. Vol. 125, No. 23, Article number 3091, JPN6024016703, 2015, ISSN: 0005316791 *
ENLI LIU ET AL., LEUKEMIA, vol. 32, no. 2, JPN6024016702, 2018, pages 520 - 531, ISSN: 0005462188 *
NINA SHAH ET AL., PLOS ONE, vol. Vol. 8, Issue 10, Article e76781, JPN6024016705, 2013, ISSN: 0005316793 *
今井 千速: "科学研究費助成事業（科学研究費補助金）研究成果報告書 遺伝子改変ＮＫ細胞の難治性白血病への臨床応用に", KAKEN 科学研究費助成事業データ ベース [ONLINE], JPN6024016706, 2014, ISSN: 0005316794 *

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TW202104252A (zh) 2021-02-01
US20220325245A1 (en) 2022-10-13
KR20220004992A (ko) 2022-01-12
BR112021019411A2 (pt) 2021-11-30
MX2021011816A (es) 2021-10-22
CN113811604A (zh) 2021-12-17
AR118510A1 (es) 2021-10-20
EP3947647A4 (en) 2023-01-04
CA3135407A1 (en) 2020-10-08
EP3947647A1 (en) 2022-02-09
WO2020205359A1 (en) 2020-10-08
AU2020254420A1 (en) 2021-11-11
CO2021012719A2 (es) 2021-10-20

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