JP2022515192A - 等温増幅反応における非特異的増幅を低減するための方法および組成物 - Google Patents

等温増幅反応における非特異的増幅を低減するための方法および組成物 Download PDF

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JP2022515192A
JP2022515192A JP2021536015A JP2021536015A JP2022515192A JP 2022515192 A JP2022515192 A JP 2022515192A JP 2021536015 A JP2021536015 A JP 2021536015A JP 2021536015 A JP2021536015 A JP 2021536015A JP 2022515192 A JP2022515192 A JP 2022515192A
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nucleic acid
seq
inhibitory oligonucleotide
polymerase
lamp
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JP2022515192A5 (https=
Inventor
カール ゲイテリ，ジョセフ
ファン，リクシュン
ハーン ロード，ブレンナ
チャン，ユーミン
フィリップ チャレロ，ロナルド
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Alveo Technologies Inc
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Alveo Technologies Inc
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Publication of JP2022515192A publication Critical patent/JP2022515192A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
JP2021536015A 2018-12-20 2019-12-18 等温増幅反応における非特異的増幅を低減するための方法および組成物 Pending JP2022515192A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862782610P 2018-12-20 2018-12-20
US62/782,610 2018-12-20
PCT/US2019/067134 WO2020132042A1 (en) 2018-12-20 2019-12-18 Methods and compositions to reduce nonspecific amplification in isothermal amplification reactions

Publications (2)

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JP2022515192A true JP2022515192A (ja) 2022-02-17
JP2022515192A5 JP2022515192A5 (https=) 2022-12-21

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JP2021536015A Pending JP2022515192A (ja) 2018-12-20 2019-12-18 等温増幅反応における非特異的増幅を低減するための方法および組成物

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Country Link
US (1) US20220056511A1 (https=)
EP (1) EP3899023A4 (https=)
JP (1) JP2022515192A (https=)
CN (1) CN113454236A (https=)
AU (1) AU2019403220A1 (https=)
CA (1) CA3123839A1 (https=)
MX (1) MX2021007311A (https=)
WO (1) WO2020132042A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
CN109996888A (zh) 2016-09-23 2019-07-09 阿尔韦奥科技公司 用于检测分析物的方法和组合物
EP3899022A4 (en) 2018-12-20 2023-03-01 Alveo Technologies Inc. PORTABLE IMPEDANCE-BASED DIAGNOSTIC TEST SYSTEM FOR DETECTING ANALYTES
JP2023536962A (ja) * 2020-08-06 2023-08-30 エフ． ホフマン－ラ ロシュ アーゲー 重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－２）、インフルエンザａ及びインフルエンザｂの検出のための組成物及び方法
US12472492B2 (en) 2020-08-14 2025-11-18 Alveo Technologies, Inc. Systems and methods of sample depositing and testing
CN119948173A (zh) * 2022-09-07 2025-05-06 贝克顿迪金森公司 用于改进检测特异性的修饰的分子信标
PL442248A1 (pl) * 2022-09-12 2024-03-18 Genomtec Spółka Akcyjna Zestaw starterów, skład mieszaniny reakcyjnej oraz metoda wykrywania ludzkiego syncytialnego wirusa oddechowego (ang. Human Respiratory Syncital Virus) typ A i typ B (RSVA) i (RSVB)
WO2024191684A1 (en) * 2023-03-10 2024-09-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Detection of hepatitis c virus ribonucleic acid from whole blood using reverse transcription loop-mediated isothermal amplification
WO2026006021A2 (en) 2024-06-25 2026-01-02 Alveo Technologies, Inc. Systems and methods of sample depositing and testing

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007060949A1 (ja) * 2005-11-25 2007-05-31 K.K. Dnaform 核酸検出及び増幅方法
WO2007074894A1 (ja) * 2005-12-28 2007-07-05 School Corporation, Azabu Veterinary Medicine Educational Institution 標的配列の特異的高感度増幅法
JP2008516613A (ja) * 2004-10-18 2008-05-22 ブランデイズ ユニバーシティー Ｐｃｒ増幅での再現性の改善およびミスプライミングの低減のための試薬および方法
WO2012075231A1 (en) * 2010-12-03 2012-06-07 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
WO2012121225A1 (ja) * 2011-03-04 2012-09-13 株式会社カネカ 核酸の検出方法及びその方法に用いるデバイス、キット
WO2015058008A2 (en) * 2013-10-18 2015-04-23 California Institute Of Technology Enhanced nucleic acid identification and detection
JP2017537648A (ja) * 2014-12-19 2017-12-21 ブランデイズ ユニバーシティー ミスプライミング防止試薬

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070212704A1 (en) * 2005-10-03 2007-09-13 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008516613A (ja) * 2004-10-18 2008-05-22 ブランデイズ ユニバーシティー Ｐｃｒ増幅での再現性の改善およびミスプライミングの低減のための試薬および方法
WO2007060949A1 (ja) * 2005-11-25 2007-05-31 K.K. Dnaform 核酸検出及び増幅方法
WO2007074894A1 (ja) * 2005-12-28 2007-07-05 School Corporation, Azabu Veterinary Medicine Educational Institution 標的配列の特異的高感度増幅法
WO2012075231A1 (en) * 2010-12-03 2012-06-07 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
WO2012121225A1 (ja) * 2011-03-04 2012-09-13 株式会社カネカ 核酸の検出方法及びその方法に用いるデバイス、キット
WO2015058008A2 (en) * 2013-10-18 2015-04-23 California Institute Of Technology Enhanced nucleic acid identification and detection
JP2017537648A (ja) * 2014-12-19 2017-12-21 ブランデイズ ユニバーシティー ミスプライミング防止試薬

Also Published As

Publication number Publication date
CN113454236A (zh) 2021-09-28
WO2020132042A1 (en) 2020-06-25
EP3899023A1 (en) 2021-10-27
MX2021007311A (es) 2021-09-21
EP3899023A4 (en) 2022-10-12
AU2019403220A1 (en) 2021-07-15
CA3123839A1 (en) 2020-06-25
US20220056511A1 (en) 2022-02-24

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