MX2021007311A - Metodos y composiciones para reducir la amplificacion no especifica en reacciones de amplificacion isotermica. - Google Patents
Metodos y composiciones para reducir la amplificacion no especifica en reacciones de amplificacion isotermica.Info
- Publication number
- MX2021007311A MX2021007311A MX2021007311A MX2021007311A MX2021007311A MX 2021007311 A MX2021007311 A MX 2021007311A MX 2021007311 A MX2021007311 A MX 2021007311A MX 2021007311 A MX2021007311 A MX 2021007311A MX 2021007311 A MX2021007311 A MX 2021007311A
- Authority
- MX
- Mexico
- Prior art keywords
- amplification
- compositions
- methods
- reactions
- reduce nonspecific
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 3
- 238000006243 chemical reaction Methods 0.000 title abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 3
- 238000011901 isothermal amplification Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title abstract 2
- 238000007397 LAMP assay Methods 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862782610P | 2018-12-20 | 2018-12-20 | |
| PCT/US2019/067134 WO2020132042A1 (en) | 2018-12-20 | 2019-12-18 | Methods and compositions to reduce nonspecific amplification in isothermal amplification reactions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2021007311A true MX2021007311A (es) | 2021-09-21 |
Family
ID=71101681
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2021007311A MX2021007311A (es) | 2018-12-20 | 2019-12-18 | Metodos y composiciones para reducir la amplificacion no especifica en reacciones de amplificacion isotermica. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20220056511A1 (https=) |
| EP (1) | EP3899023A4 (https=) |
| JP (1) | JP2022515192A (https=) |
| CN (1) | CN113454236A (https=) |
| AU (1) | AU2019403220A1 (https=) |
| CA (1) | CA3123839A1 (https=) |
| MX (1) | MX2021007311A (https=) |
| WO (1) | WO2020132042A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10196678B2 (en) | 2014-10-06 | 2019-02-05 | ALVEO Technologies Inc. | System and method for detection of nucleic acids |
| CN109996888A (zh) | 2016-09-23 | 2019-07-09 | 阿尔韦奥科技公司 | 用于检测分析物的方法和组合物 |
| EP3899022A4 (en) | 2018-12-20 | 2023-03-01 | Alveo Technologies Inc. | PORTABLE IMPEDANCE-BASED DIAGNOSTIC TEST SYSTEM FOR DETECTING ANALYTES |
| JP2023536962A (ja) * | 2020-08-06 | 2023-08-30 | エフ． ホフマン－ラ ロシュ アーゲー | 重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－２）、インフルエンザａ及びインフルエンザｂの検出のための組成物及び方法 |
| US12472492B2 (en) | 2020-08-14 | 2025-11-18 | Alveo Technologies, Inc. | Systems and methods of sample depositing and testing |
| CN119948173A (zh) * | 2022-09-07 | 2025-05-06 | 贝克顿迪金森公司 | 用于改进检测特异性的修饰的分子信标 |
| PL442248A1 (pl) * | 2022-09-12 | 2024-03-18 | Genomtec Spółka Akcyjna | Zestaw starterów, skład mieszaniny reakcyjnej oraz metoda wykrywania ludzkiego syncytialnego wirusa oddechowego (ang. Human Respiratory Syncital Virus) typ A i typ B (RSVA) i (RSVB) |
| WO2024191684A1 (en) * | 2023-03-10 | 2024-09-19 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Detection of hepatitis c virus ribonucleic acid from whole blood using reverse transcription loop-mediated isothermal amplification |
| WO2026006021A2 (en) | 2024-06-25 | 2026-01-02 | Alveo Technologies, Inc. | Systems and methods of sample depositing and testing |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE541055T1 (de) * | 2004-10-18 | 2012-01-15 | Univ Brandeis | Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation |
| US20070212704A1 (en) * | 2005-10-03 | 2007-09-13 | Applera Corporation | Compositions, methods, and kits for amplifying nucleic acids |
| US8097414B2 (en) * | 2005-11-25 | 2012-01-17 | K. K. Dnaform | Method for detecting and amplifying nucleic acid |
| WO2007074894A1 (ja) * | 2005-12-28 | 2007-07-05 | School Corporation, Azabu Veterinary Medicine Educational Institution | 標的配列の特異的高感度増幅法 |
| CN103459612A (zh) * | 2010-12-03 | 2013-12-18 | 布兰代斯大学 | 用于检测野生型群体中的核酸突变体的方法和试剂盒 |
| SG193315A1 (en) * | 2011-03-04 | 2013-10-30 | Kaneka Corp | Nucleic acid detection method, and device and kit for use in same |
| EP3058100A4 (en) * | 2013-10-18 | 2017-04-19 | California Institute of Technology | Enhanced nucleic acid identification and detection |
| CA2971200A1 (en) * | 2014-12-19 | 2016-06-23 | Brandeis University | Mispriming prevention reagents |
-
2019
- 2019-12-18 MX MX2021007311A patent/MX2021007311A/es unknown
- 2019-12-18 CA CA3123839A patent/CA3123839A1/en active Pending
- 2019-12-18 JP JP2021536015A patent/JP2022515192A/ja active Pending
- 2019-12-18 WO PCT/US2019/067134 patent/WO2020132042A1/en not_active Ceased
- 2019-12-18 CN CN201980092722.5A patent/CN113454236A/zh active Pending
- 2019-12-18 EP EP19899953.4A patent/EP3899023A4/en not_active Withdrawn
- 2019-12-18 US US17/416,105 patent/US20220056511A1/en not_active Abandoned
- 2019-12-18 AU AU2019403220A patent/AU2019403220A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CN113454236A (zh) | 2021-09-28 |
| WO2020132042A1 (en) | 2020-06-25 |
| JP2022515192A (ja) | 2022-02-17 |
| EP3899023A1 (en) | 2021-10-27 |
| EP3899023A4 (en) | 2022-10-12 |
| AU2019403220A1 (en) | 2021-07-15 |
| CA3123839A1 (en) | 2020-06-25 |
| US20220056511A1 (en) | 2022-02-24 |
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