CN113454236A - 减少等温扩增反应中非特异性扩增的方法和组合物 - Google Patents

减少等温扩增反应中非特异性扩增的方法和组合物 Download PDF

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Publication number
CN113454236A
CN113454236A CN201980092722.5A CN201980092722A CN113454236A CN 113454236 A CN113454236 A CN 113454236A CN 201980092722 A CN201980092722 A CN 201980092722A CN 113454236 A CN113454236 A CN 113454236A
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seq
nucleic acid
nucleotide sequence
oligonucleotide
lamp
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CN201980092722.5A
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Chinese (zh)
Inventor
约瑟夫·卡尔·盖特里
方日勋
布伦纳·赫恩·洛德
蒋玉民
罗纳德·菲利普·基亚雷洛
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Alveo Technologies Inc
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Alveo Technologies Inc
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Publication of CN113454236A publication Critical patent/CN113454236A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
CN201980092722.5A 2018-12-20 2019-12-18 减少等温扩增反应中非特异性扩增的方法和组合物 Pending CN113454236A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862782610P 2018-12-20 2018-12-20
US62/782,610 2018-12-20
PCT/US2019/067134 WO2020132042A1 (en) 2018-12-20 2019-12-18 Methods and compositions to reduce nonspecific amplification in isothermal amplification reactions

Publications (1)

Publication Number Publication Date
CN113454236A true CN113454236A (zh) 2021-09-28

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CN201980092722.5A Pending CN113454236A (zh) 2018-12-20 2019-12-18 减少等温扩增反应中非特异性扩增的方法和组合物

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Country Link
US (1) US20220056511A1 (https=)
EP (1) EP3899023A4 (https=)
JP (1) JP2022515192A (https=)
CN (1) CN113454236A (https=)
AU (1) AU2019403220A1 (https=)
CA (1) CA3123839A1 (https=)
MX (1) MX2021007311A (https=)
WO (1) WO2020132042A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
CN109996888A (zh) 2016-09-23 2019-07-09 阿尔韦奥科技公司 用于检测分析物的方法和组合物
EP3899022A4 (en) 2018-12-20 2023-03-01 Alveo Technologies Inc. PORTABLE IMPEDANCE-BASED DIAGNOSTIC TEST SYSTEM FOR DETECTING ANALYTES
JP2023536962A (ja) * 2020-08-06 2023-08-30 エフ． ホフマン－ラ ロシュ アーゲー 重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－２）、インフルエンザａ及びインフルエンザｂの検出のための組成物及び方法
US12472492B2 (en) 2020-08-14 2025-11-18 Alveo Technologies, Inc. Systems and methods of sample depositing and testing
CN119948173A (zh) * 2022-09-07 2025-05-06 贝克顿迪金森公司 用于改进检测特异性的修饰的分子信标
PL442248A1 (pl) * 2022-09-12 2024-03-18 Genomtec Spółka Akcyjna Zestaw starterów, skład mieszaniny reakcyjnej oraz metoda wykrywania ludzkiego syncytialnego wirusa oddechowego (ang. Human Respiratory Syncital Virus) typ A i typ B (RSVA) i (RSVB)
WO2024191684A1 (en) * 2023-03-10 2024-09-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Detection of hepatitis c virus ribonucleic acid from whole blood using reverse transcription loop-mediated isothermal amplification
WO2026006021A2 (en) 2024-06-25 2026-01-02 Alveo Technologies, Inc. Systems and methods of sample depositing and testing

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060177842A1 (en) * 2004-10-18 2006-08-10 Wangh Lawrence J Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
CN101321877A (zh) * 2005-10-03 2008-12-10 阿普里拉股份有限公司 用于扩增核酸的组合物、方法和试剂盒
WO2015058008A2 (en) * 2013-10-18 2015-04-23 California Institute Of Technology Enhanced nucleic acid identification and detection
WO2016100335A1 (en) * 2014-12-19 2016-06-23 Brandeis University Mispriming prevention reagents

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8097414B2 (en) * 2005-11-25 2012-01-17 K. K. Dnaform Method for detecting and amplifying nucleic acid
WO2007074894A1 (ja) * 2005-12-28 2007-07-05 School Corporation, Azabu Veterinary Medicine Educational Institution 標的配列の特異的高感度増幅法
CN103459612A (zh) * 2010-12-03 2013-12-18 布兰代斯大学 用于检测野生型群体中的核酸突变体的方法和试剂盒
SG193315A1 (en) * 2011-03-04 2013-10-30 Kaneka Corp Nucleic acid detection method, and device and kit for use in same

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060177842A1 (en) * 2004-10-18 2006-08-10 Wangh Lawrence J Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
CN101076608A (zh) * 2004-10-18 2007-11-21 布兰迪斯大学 在pcr扩增中提高再现性和降低引物错导的试剂和方法
CN101321877A (zh) * 2005-10-03 2008-12-10 阿普里拉股份有限公司 用于扩增核酸的组合物、方法和试剂盒
WO2015058008A2 (en) * 2013-10-18 2015-04-23 California Institute Of Technology Enhanced nucleic acid identification and detection
WO2016100335A1 (en) * 2014-12-19 2016-06-23 Brandeis University Mispriming prevention reagents

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GHAITH: "Development of a reverse transcription loop-mediated isothermal amplification assay for rapid detection of foot-and-mouth disease virus", MASTER OF SCIENCE THESIS, pages 8 *

Also Published As

Publication number Publication date
WO2020132042A1 (en) 2020-06-25
JP2022515192A (ja) 2022-02-17
EP3899023A1 (en) 2021-10-27
MX2021007311A (es) 2021-09-21
EP3899023A4 (en) 2022-10-12
AU2019403220A1 (en) 2021-07-15
CA3123839A1 (en) 2020-06-25
US20220056511A1 (en) 2022-02-24

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