CA3123839A1 - Methods and compositions to reduce nonspecific amplification in isothermal amplification reactions - Google Patents

Methods and compositions to reduce nonspecific amplification in isothermal amplification reactions Download PDF

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Publication number
CA3123839A1
CA3123839A1 CA3123839A CA3123839A CA3123839A1 CA 3123839 A1 CA3123839 A1 CA 3123839A1 CA 3123839 A CA3123839 A CA 3123839A CA 3123839 A CA3123839 A CA 3123839A CA 3123839 A1 CA3123839 A1 CA 3123839A1
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CA
Canada
Prior art keywords
nucleic acid
seq
inhibitor oligonucleotide
nucleotide sequence
aqueous solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3123839A
Other languages
English (en)
French (fr)
Inventor
Joseph Carl GAITERI
Rixun Fang
Brenna Hearn Lord
Yuh-Min Chiang
Ronald Phillip Chiarello
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alveo Technologies Inc
Original Assignee
Alveo Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alveo Technologies Inc filed Critical Alveo Technologies Inc
Publication of CA3123839A1 publication Critical patent/CA3123839A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
CA3123839A 2018-12-20 2019-12-18 Methods and compositions to reduce nonspecific amplification in isothermal amplification reactions Pending CA3123839A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862782610P 2018-12-20 2018-12-20
US62/782,610 2018-12-20
PCT/US2019/067134 WO2020132042A1 (en) 2018-12-20 2019-12-18 Methods and compositions to reduce nonspecific amplification in isothermal amplification reactions

Publications (1)

Publication Number Publication Date
CA3123839A1 true CA3123839A1 (en) 2020-06-25

Family

ID=71101681

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3123839A Pending CA3123839A1 (en) 2018-12-20 2019-12-18 Methods and compositions to reduce nonspecific amplification in isothermal amplification reactions

Country Status (8)

Country Link
US (1) US20220056511A1 (https=)
EP (1) EP3899023A4 (https=)
JP (1) JP2022515192A (https=)
CN (1) CN113454236A (https=)
AU (1) AU2019403220A1 (https=)
CA (1) CA3123839A1 (https=)
MX (1) MX2021007311A (https=)
WO (1) WO2020132042A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
CN109996888A (zh) 2016-09-23 2019-07-09 阿尔韦奥科技公司 用于检测分析物的方法和组合物
EP3899022A4 (en) 2018-12-20 2023-03-01 Alveo Technologies Inc. PORTABLE IMPEDANCE-BASED DIAGNOSTIC TEST SYSTEM FOR DETECTING ANALYTES
JP2023536962A (ja) * 2020-08-06 2023-08-30 エフ． ホフマン－ラ ロシュ アーゲー 重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－２）、インフルエンザａ及びインフルエンザｂの検出のための組成物及び方法
US12472492B2 (en) 2020-08-14 2025-11-18 Alveo Technologies, Inc. Systems and methods of sample depositing and testing
CN119948173A (zh) * 2022-09-07 2025-05-06 贝克顿迪金森公司 用于改进检测特异性的修饰的分子信标
PL442248A1 (pl) * 2022-09-12 2024-03-18 Genomtec Spółka Akcyjna Zestaw starterów, skład mieszaniny reakcyjnej oraz metoda wykrywania ludzkiego syncytialnego wirusa oddechowego (ang. Human Respiratory Syncital Virus) typ A i typ B (RSVA) i (RSVB)
WO2024191684A1 (en) * 2023-03-10 2024-09-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Detection of hepatitis c virus ribonucleic acid from whole blood using reverse transcription loop-mediated isothermal amplification
WO2026006021A2 (en) 2024-06-25 2026-01-02 Alveo Technologies, Inc. Systems and methods of sample depositing and testing

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE541055T1 (de) * 2004-10-18 2012-01-15 Univ Brandeis Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation
US20070212704A1 (en) * 2005-10-03 2007-09-13 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
US8097414B2 (en) * 2005-11-25 2012-01-17 K. K. Dnaform Method for detecting and amplifying nucleic acid
WO2007074894A1 (ja) * 2005-12-28 2007-07-05 School Corporation, Azabu Veterinary Medicine Educational Institution 標的配列の特異的高感度増幅法
CN103459612A (zh) * 2010-12-03 2013-12-18 布兰代斯大学 用于检测野生型群体中的核酸突变体的方法和试剂盒
SG193315A1 (en) * 2011-03-04 2013-10-30 Kaneka Corp Nucleic acid detection method, and device and kit for use in same
EP3058100A4 (en) * 2013-10-18 2017-04-19 California Institute of Technology Enhanced nucleic acid identification and detection
CA2971200A1 (en) * 2014-12-19 2016-06-23 Brandeis University Mispriming prevention reagents

Also Published As

Publication number Publication date
CN113454236A (zh) 2021-09-28
WO2020132042A1 (en) 2020-06-25
JP2022515192A (ja) 2022-02-17
EP3899023A1 (en) 2021-10-27
MX2021007311A (es) 2021-09-21
EP3899023A4 (en) 2022-10-12
AU2019403220A1 (en) 2021-07-15
US20220056511A1 (en) 2022-02-24

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