JP2022025140A5 - - Google Patents

Download PDF

Info

Publication number
JP2022025140A5
JP2022025140A5 JP2021183083A JP2021183083A JP2022025140A5 JP 2022025140 A5 JP2022025140 A5 JP 2022025140A5 JP 2021183083 A JP2021183083 A JP 2021183083A JP 2021183083 A JP2021183083 A JP 2021183083A JP 2022025140 A5 JP2022025140 A5 JP 2022025140A5
Authority
JP
Japan
Prior art keywords
capture probe
nucleic acid
sequence
dna fragment
multifunctional
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2021183083A
Other languages
English (en)
Japanese (ja)
Other versions
JP7377247B2 (ja
JP2022025140A (ja
Filing date
Publication date
Priority claimed from JP2021025425A external-priority patent/JP7223790B2/ja
Application filed filed Critical
Publication of JP2022025140A publication Critical patent/JP2022025140A/ja
Publication of JP2022025140A5 publication Critical patent/JP2022025140A5/ja
Priority to JP2023184894A priority Critical patent/JP7710016B2/ja
Application granted granted Critical
Publication of JP7377247B2 publication Critical patent/JP7377247B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2021183083A 2012-12-10 2021-11-10 標的化ゲノム解析のための方法 Active JP7377247B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023184894A JP7710016B2 (ja) 2012-12-10 2023-10-27 標的化ゲノム解析のための方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261735417P 2012-12-10 2012-12-10
US61/735,417 2012-12-10
US201361794049P 2013-03-15 2013-03-15
US61/794,049 2013-03-15
JP2021025425A JP7223790B2 (ja) 2012-12-10 2021-02-19 標的化ゲノム解析のための方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2021025425A Division JP7223790B2 (ja) 2012-12-10 2021-02-19 標的化ゲノム解析のための方法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023184894A Division JP7710016B2 (ja) 2012-12-10 2023-10-27 標的化ゲノム解析のための方法

Publications (3)

Publication Number Publication Date
JP2022025140A JP2022025140A (ja) 2022-02-09
JP2022025140A5 true JP2022025140A5 (https=) 2022-09-09
JP7377247B2 JP7377247B2 (ja) 2023-11-09

Family

ID=50934888

Family Applications (5)

Application Number Title Priority Date Filing Date
JP2015545927A Active JP6410726B2 (ja) 2012-12-10 2013-12-10 標的化ゲノム解析のための方法
JP2018178774A Active JP6842450B2 (ja) 2012-12-10 2018-09-25 標的化ゲノム解析のための方法
JP2021025425A Active JP7223790B2 (ja) 2012-12-10 2021-02-19 標的化ゲノム解析のための方法
JP2021183083A Active JP7377247B2 (ja) 2012-12-10 2021-11-10 標的化ゲノム解析のための方法
JP2023184894A Active JP7710016B2 (ja) 2012-12-10 2023-10-27 標的化ゲノム解析のための方法

Family Applications Before (3)

Application Number Title Priority Date Filing Date
JP2015545927A Active JP6410726B2 (ja) 2012-12-10 2013-12-10 標的化ゲノム解析のための方法
JP2018178774A Active JP6842450B2 (ja) 2012-12-10 2018-09-25 標的化ゲノム解析のための方法
JP2021025425A Active JP7223790B2 (ja) 2012-12-10 2021-02-19 標的化ゲノム解析のための方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2023184894A Active JP7710016B2 (ja) 2012-12-10 2023-10-27 標的化ゲノム解析のための方法

Country Status (8)

Country Link
US (5) US9932576B2 (https=)
EP (3) EP2929056A4 (https=)
JP (5) JP6410726B2 (https=)
CN (3) CN111254500B (https=)
CA (1) CA2892646A1 (https=)
HK (1) HK1213944A1 (https=)
SG (3) SG10202009015SA (https=)
WO (1) WO2014093330A1 (https=)

Families Citing this family (146)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
WO2011127099A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2013059746A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
EP2807292B1 (en) 2012-01-26 2019-05-22 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN104781421B (zh) 2012-09-04 2020-06-05 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP4592400A3 (en) 2012-10-17 2025-10-29 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
CN111254500B (zh) 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
CA2901907C (en) * 2013-02-20 2023-06-20 Emory University Methods of sequencing nucleic acids in mixtures and compositions related thereto
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
CA2916662C (en) 2013-06-25 2022-03-08 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
SG11201600853UA (en) 2013-08-05 2016-03-30 Twist Bioscience Corp De novo synthesized gene libraries
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
JP7441584B6 (ja) * 2014-08-22 2024-03-15 レゾリューション バイオサイエンス, インコーポレイテッド 無細胞DNA(cfDNA)の定量的遺伝子解析のための方法
CN115029342B (zh) * 2014-08-22 2025-04-08 分析生物科学有限公司 用于无细胞dna的定量遗传分析的方法
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
WO2016093838A1 (en) * 2014-12-11 2016-06-16 New England Biolabs, Inc. Enrichment of target sequences
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US20180119214A1 (en) * 2015-03-31 2018-05-03 Fred Hutchinson Cancer Research Center Compositions and methods for target nucleic acid molecule enrichment
EP3901282B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3307908B1 (en) 2015-06-09 2019-09-11 Life Technologies Corporation Methods for molecular tagging
EP3350314A4 (en) 2015-09-18 2019-02-06 Twist Bioscience Corporation OLIGONUCLEIC ACID VARIANT LIBRARIES VARIANT AND SYNTHESIS THEREOF
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
JP6913089B2 (ja) * 2015-11-11 2021-08-04 レゾリューション バイオサイエンス, インコーポレイテッド Dnaライブラリーの高効率構築
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
BR112018016240A2 (pt) 2016-02-11 2019-01-02 Htg Molecular Diagnostics Inc método de sequência alvo direta que utiliza proteção da nuclease
US10240196B2 (en) 2016-05-27 2019-03-26 Agilent Technologies, Inc. Transposase-random priming DNA sample preparation
CN107058310A (zh) * 2016-08-12 2017-08-18 艾吉泰康生物科技(北京)有限公司 一种提高基因低频突变检测灵敏度的扩增子文库构建方法
EP3500672A4 (en) 2016-08-22 2020-05-20 Twist Bioscience Corporation DE NOVO SYNTHETIZED NUCLEIC ACID LIBRARIES
US11319594B2 (en) * 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
EP4556433A3 (en) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
FI3638776T3 (fi) 2017-06-14 2025-12-09 Univ Dresden Tech Menetelmiä ja välineitä genomien geneettiseen muokkaamiseen hyödyntäen suunnittelu-DNA:ta rekombinoivia entsyymejä
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
WO2019113563A1 (en) * 2017-12-09 2019-06-13 Viome, Inc. Methods for nucleic acid library creation
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
CA3088012A1 (en) * 2018-01-10 2019-07-18 Memorial Sloan Kettering Cancer Center Generating configurable text strings based on raw genomic data
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
CN108866155B (zh) * 2018-06-11 2022-07-26 中国农业科学院深圳农业基因组研究所 一种下一代测序文库的制备方法
EP3810805A4 (en) * 2018-06-25 2022-03-23 Lucence Life Sciences Pte Ltd. METHODS FOR DETECTING AND QUANTIFYING GENETIC MODIFICATIONS
US12473592B2 (en) 2018-06-25 2025-11-18 Lucence Life Sciences Pte. Ltd. Method for the detection and quantification of genetic alterations
DK3827011T3 (da) * 2018-07-24 2024-07-08 Salish Bioscience Inc Fremgangsmåder og sammensætning til målrettet genomisk analyse
CA3106677A1 (en) * 2018-08-17 2020-02-20 Dana-Farber Cancer Institue, Inc. Dna amplification method for probe generation
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
JP7124877B2 (ja) 2018-09-07 2022-08-24 富士通株式会社 特定方法、特定プログラムおよび情報処理装置
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
WO2020139871A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3906318A1 (en) * 2019-01-06 2021-11-10 10X Genomics, Inc. Methods and systems for enrichment of barcodes
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CA3144106A1 (en) 2019-07-03 2021-01-07 Integrated Dna Technologies, Inc. Identification, characterization, and quantitation of crispr-introduced double-stranded dna break repairs
CA3145114A1 (en) * 2019-08-16 2021-02-25 Chris Raymond Targeted hybrid capture methods for determination of t cell repertoires
EP4034566A4 (en) 2019-09-23 2024-01-24 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CRTH2
JP2022548783A (ja) 2019-09-23 2022-11-21 ツイスト バイオサイエンス コーポレーション 単一ドメイン抗体のバリアント核酸ライブラリー
CN119193713A (zh) 2019-10-24 2024-12-27 合成Dna技术公司 修饰的双链供体模板
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP3831939A1 (en) * 2019-12-06 2021-06-09 Technische Universität Dresden Fusion of site-specific recombinases for efficient and specific genome editing
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4153776B1 (en) 2020-05-22 2025-03-05 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
EP4600376A3 (en) 2020-06-02 2025-10-22 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
EP4165207B1 (en) 2020-06-10 2024-09-25 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
JP2023535407A (ja) * 2020-07-23 2023-08-17 インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド 「CTL-seq」(CRISPR Tag Linear-seq)と称するヌクレアーゼオン/オフターゲット編集位置を指定するための方法
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
WO2022061150A2 (en) 2020-09-18 2022-03-24 10X Geonomics, Inc. Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
CN112481371A (zh) * 2020-12-11 2021-03-12 长沙金域医学检验实验室有限公司 一种检测plp1基因拷贝数变异的试剂盒
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4421491A3 (en) 2021-02-19 2024-11-27 10X Genomics, Inc. Method of using a modular assay support device
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
JP2024512156A (ja) * 2021-03-30 2024-03-18 レゾリューション バイオサイエンス, インコーポレイテッド 複数のライブラリーを同時に遺伝子解析するための組成物及び方法
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
US12176070B2 (en) * 2021-04-19 2024-12-24 University Of Utah Research Foundation Systems and methods for facilitating rapid genome sequence analysis
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
CN113684259B (zh) * 2021-08-20 2024-08-30 陕西佰美基因股份有限公司 基于非标记探针熔解技术检测cyp2d6基因拷贝数的方法、引物对与非标记探针组合及试剂盒
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
CN114898802B (zh) * 2022-07-14 2022-09-30 臻和(北京)生物科技有限公司 基于血浆游离dna甲基化测序数据的末端序列频率分布特征确定方法、评价方法及装置
EP4482979B1 (en) 2022-11-09 2025-10-01 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
WO2025147648A1 (en) * 2024-01-05 2025-07-10 Harbinger Health, Inc. Probe blockers for enrichment of target nucleic acid sequences
WO2026010830A1 (en) * 2024-07-01 2026-01-08 Watchmaker Genomics, Inc. Methods of polymerase free dna end repair

Family Cites Families (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591582A (en) 1985-07-23 1997-01-07 The Board Of Rijks Universiteit Leiden Methods for detecting activated RAS oncogenes
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
WO1995025180A1 (en) 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US5514551A (en) 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
US5888731A (en) 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US6025133A (en) 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
EP0991779A1 (en) 1997-08-28 2000-04-12 The Perkin-Elmer Corporation Improved detection of mutations in nucleic acids by chemical cleavage
JP2002509443A (ja) 1997-10-31 2002-03-26 ジェン−プローブ・インコーポレーテッド 核酸検出法
US6480791B1 (en) * 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US7081527B2 (en) 2000-09-12 2006-07-25 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of Cryptosporidium parvum organisms in a test sample
US6812341B1 (en) 2001-05-11 2004-11-02 Ambion, Inc. High efficiency mRNA isolation methods and compositions
AU2002365028A1 (en) 2001-07-17 2003-06-30 Stratagene Methods for detection of a target nucleic acid using multi-subunit probes
WO2003020979A1 (en) 2001-08-31 2003-03-13 Rosetta Inpharmactis Llc. Methods for preparing nucleic acid samples
US7361821B2 (en) 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
JP4441623B2 (ja) 2002-12-11 2010-03-31 学校法人慶應義塾 対応付け分子およびその構成要素のライブラリーの製造方法および利用方法
TW200506052A (en) 2003-05-07 2005-02-16 Takara Bio Inc Method of analyzing DNA region
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
EP2476761A3 (en) 2005-07-07 2012-10-17 Athlomics Pty Ltd Polynucleotide marker genes and their expression, for diagnosis of endotoxemia
GB0522310D0 (en) * 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20070117089A1 (en) 2005-11-21 2007-05-24 Croker Kevin M Sol-gel coated glass microspheres for use in bioassay
US7572643B2 (en) 2005-11-21 2009-08-11 E. I. Du Pont De Nemours And Company Nanoparticle composite-coated glass microspheres for use in bioassays
JP5106416B2 (ja) 2006-01-06 2012-12-26 アジレント・テクノロジーズ・インク 濃縮(packed)DNAポリメラーゼを含む核酸複製のための反応緩衝液組成物
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US8383338B2 (en) 2006-04-24 2013-02-26 Roche Nimblegen, Inc. Methods and systems for uniform enrichment of genomic regions
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008045158A1 (en) 2006-10-10 2008-04-17 Illumina, Inc. Compositions and methods for representational selection of nucleic acids fro complex mixtures using hybridization
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US12180549B2 (en) 2007-07-23 2024-12-31 The Chinese University Of Hong Kong Diagnosing fetal chromosomal aneuploidy using genomic sequencing
WO2009023676A1 (en) 2007-08-12 2009-02-19 Integrated Dna Technologies, Inc. Microarray system with improved sequence specificity
US9388457B2 (en) * 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
EP2227563B1 (en) 2007-12-05 2012-06-06 Complete Genomics, Inc. Efficient base determination in sequencing reactions
WO2009091798A1 (en) * 2008-01-16 2009-07-23 Helicos Biosciences Corporation Quantitative genetic analysis
US8202691B2 (en) * 2008-01-25 2012-06-19 Illumina, Inc. Uniform fragmentation of DNA using binding proteins
EP2245198A1 (en) 2008-02-04 2010-11-03 Massachusetts Institute of Technology Selection of nucleic acids by solution hybridization to oligonucleotide baits
DE102008008313A1 (de) 2008-02-07 2009-08-13 Qiagen Gmbh Amplifikation von bisulfitierten Nukleinsäuren
CA2718905A1 (en) * 2008-03-17 2009-09-24 Expressive Research B.V. Expression-linked gene discovery
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
CA2750054C (en) 2008-10-24 2018-05-29 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2010068856A1 (en) 2008-12-11 2010-06-17 Trustees Of Boston University Isolation of rna
SG174617A1 (en) * 2009-04-02 2011-10-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US20110003301A1 (en) * 2009-05-08 2011-01-06 Life Technologies Corporation Methods for detecting genetic variations in dna samples
PT2669387T (pt) 2009-08-25 2016-09-20 Illumina Inc Métodos para selecção e amplificação de polinucleótidos
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10388403B2 (en) 2010-01-19 2019-08-20 Verinata Health, Inc. Analyzing copy number variation in the detection of cancer
US10378064B1 (en) 2010-04-16 2019-08-13 Chronix Biomedical Analyzing circulating nucleic acids to identify a biomarker representative of cancer presented by a patient population
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US8828688B2 (en) * 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP2580378A4 (en) * 2010-06-08 2014-01-01 Nugen Technologies Inc MULTIPLEX SEQUENCING METHODS AND COMPOSITION
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
EP2619329B1 (en) * 2010-09-24 2019-05-22 The Board of Trustees of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
CN105243295B (zh) 2010-11-30 2018-08-17 香港中文大学 与癌症相关的遗传或分子畸变的检测
KR102759126B1 (ko) 2010-12-30 2025-01-24 파운데이션 메디신 인코포레이티드 종양 샘플의 다유전자 분석의 최적화
RU2671980C2 (ru) 2011-02-09 2018-11-08 Натера, Инк. Способы неинвазивного пренатального установления плоидности
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
US9212193B2 (en) 2011-04-05 2015-12-15 E I Du Pont De Nemours And Company Amine-accelerated process for the surface treatment of colloidal silica and products thereof
RS57837B1 (sr) 2011-04-12 2018-12-31 Verinata Health Inc Razrešavanje genomskih frakcija upotrebom broja kopija polimorfizama
ES2986436T3 (es) 2011-04-15 2024-11-11 Univ Johns Hopkins Sistema de secuenciación segura
WO2013056178A2 (en) 2011-10-14 2013-04-18 Foundation Medicine, Inc. Novel estrogen receptor mutations and uses thereof
CN103103624B (zh) 2011-11-15 2014-12-31 深圳华大基因科技服务有限公司 高通量测序文库的构建方法及其应用
US10227587B2 (en) 2012-01-10 2019-03-12 Berry Genomics Co., Ltd. Method for constructing a plasma DNA sequencing library
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
PT2828218T (pt) 2012-03-20 2020-11-11 Univ Washington Through Its Center For Commercialization Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus
US20130288915A1 (en) 2012-04-27 2013-10-31 Htg Molecular Diagnostics, Inc. Compositions and methods for alk molecular testing
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
ES2769796T3 (es) 2012-07-03 2020-06-29 Integrated Dna Tech Inc Oligonucleótidos de bloqueo aumentados en Tm y señuelos para un enriquecimiento de diana mejorado y una selección fuera de diana reducida
ES2742285T3 (es) 2012-07-31 2020-02-13 Novartis Ag Marcadores asociados con la sensibilidad a inhibidores del doble minuto humano 2 (MDM2)
AU2013296237B2 (en) 2012-08-03 2019-05-16 Foundation Medicine, Inc. Human papilloma virus as predictor of cancer prognosis
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN104781421B (zh) 2012-09-04 2020-06-05 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
WO2014052487A1 (en) 2012-09-28 2014-04-03 Cepheid Two-primer pcr for microrna multiplex assay
CA3120521A1 (en) 2012-10-04 2014-04-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2014071295A1 (en) 2012-11-02 2014-05-08 Enzymatics Inc. Methods and kits for nucleic acid sample preparation for sequencing
EP2914621B1 (en) 2012-11-05 2023-06-07 Foundation Medicine, Inc. Novel ntrk1 fusion molecules and uses thereof
CN111254500B (zh) 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
EP2931922B1 (en) 2012-12-14 2018-10-17 Chronix Biomedical Personalized biomarkers for cancer
US11158425B2 (en) 2013-01-05 2021-10-26 Foundation Medicine, Inc. System and method for managing genomic information
WO2014116881A1 (en) 2013-01-23 2014-07-31 Reproductive Genetics And Technology Solutions, Llc Compositions and methods for genetic analysis of embryos
US10745686B2 (en) 2013-02-08 2020-08-18 Qiagen Gmbh Method for separating DNA by size
WO2014145078A1 (en) 2013-03-15 2014-09-18 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
WO2014151117A1 (en) 2013-03-15 2014-09-25 The Board Of Trustees Of The Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
ES3022582T3 (en) 2013-05-10 2025-05-28 Found Medicine Inc Analysis of genetic variants
US20150072344A1 (en) 2013-09-10 2015-03-12 Imdaptive Incorporated Barcoded Universal Marker Indicator (BUMI) Tags
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
CN103668471B (zh) 2013-12-19 2015-09-30 上海交通大学 一种构建dna高通量测序文库的方法及其配套试剂盒
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
EP3363904B1 (en) 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
EP3114231B1 (en) 2014-03-03 2019-01-02 Swift Biosciences, Inc. Enhanced adaptor ligation
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
CA2957633A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
CN115029342B (zh) 2014-08-22 2025-04-08 分析生物科学有限公司 用于无细胞dna的定量遗传分析的方法
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
EP3842544A1 (en) 2014-09-05 2021-06-30 QIAGEN GmbH Preparation of adapter-ligated amplicons
DK3192869T3 (da) 2014-09-12 2019-05-20 Mgi Tech Co Ltd Isoleret oligonukleotid og anvendelse deraf i nukleinsyresekvensering
CN107075730A (zh) 2014-09-12 2017-08-18 利兰·斯坦福青年大学托管委员会 循环核酸的鉴定及用途
EP3227464B1 (en) 2014-12-05 2022-04-20 Foundation Medicine, Inc. Multigene analysis of tumor samples
EP3502273B1 (en) 2014-12-12 2020-07-08 Verinata Health, Inc. Cell-free dna fragment
EP3766986B1 (en) 2014-12-31 2022-06-01 Guardant Health, Inc. Detection and treatment of disease exhibiting disease cell heterogeneity and systems and methods for communicating test results
HUE058263T2 (hu) 2015-02-10 2022-07-28 Univ Hong Kong Chinese Mutációk detektálása rákszûrési és magzatelemzési célból
IL305462A (en) 2015-07-23 2023-10-01 Univ Hong Kong Chinese DNA fragmentation pattern analysis suitable clean
US10954609B2 (en) 2015-07-29 2021-03-23 Dupont Safety & Construction, Inc. Yarn from polymers having different decomposition temperatures and process for forming same
JP6991134B2 (ja) 2015-10-09 2022-01-12 ガーダント ヘルス, インコーポレイテッド 無細胞dnaを使用する集団ベースの処置レコメンダ
WO2017062970A1 (en) 2015-10-10 2017-04-13 Guardant Health, Inc. Methods and applications of gene fusion detection in cell-free dna analysis
JP6913089B2 (ja) 2015-11-11 2021-08-04 レゾリューション バイオサイエンス, インコーポレイテッド Dnaライブラリーの高効率構築
US10095831B2 (en) 2016-02-03 2018-10-09 Verinata Health, Inc. Using cell-free DNA fragment size to determine copy number variations
CN114959918A (zh) 2016-02-29 2022-08-30 基础医疗股份有限公司 用于评估肿瘤突变负荷的方法和系统
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
US11319594B2 (en) 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
EP3461274B1 (en) 2016-09-30 2020-11-04 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
IL307463B2 (en) 2016-11-17 2026-01-01 Lgc Clinical Diagnostics Inc Methods for preparing DNA reference material and controls
US11268137B2 (en) 2016-12-09 2022-03-08 Boreal Genomics, Inc. Linked ligation
EP3885445B1 (en) 2017-04-14 2023-08-23 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
JP2022513124A (ja) 2018-11-21 2022-02-07 アヴィダ バイオメッド, インコーポレイテッド 標的化された核酸ライブラリー形成のための方法
WO2022055984A1 (en) 2020-09-08 2022-03-17 Resolution Bioscience, Inc. Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis
JP2024512156A (ja) 2021-03-30 2024-03-18 レゾリューション バイオサイエンス, インコーポレイテッド 複数のライブラリーを同時に遺伝子解析するための組成物及び方法

Similar Documents

Publication Publication Date Title
JP2022025140A5 (https=)
JP2023182855A5 (https=)
US11697843B2 (en) Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
JP7377247B2 (ja) 標的化ゲノム解析のための方法
JP7514263B2 (ja) 試料核酸にアダプターを付着する方法
US9745614B2 (en) Reduced representation bisulfite sequencing with diversity adaptors
CN107075513B (zh) 分离的寡核苷酸及其在核酸测序中的用途
JP4289443B2 (ja) Pcrの過程でdna断片の増幅を抑制する方法
US7510829B2 (en) Multiplex PCR
US8986958B2 (en) Methods for generating target specific probes for solution based capture
JP2022105062A5 (https=)
JP6219944B2 (ja) 5’保護に依存した増幅
JP2021073252A5 (https=)
US20090047680A1 (en) Methods and compositions for high-throughput bisulphite dna-sequencing and utilities
US20140274729A1 (en) Methods, compositions and kits for generation of stranded rna or dna libraries
CN113106145B (zh) 用于制备核酸文库的组合物和方法
JP2024161413A (ja) 核酸ライブラリを生成する方法ならびにそれを実施するための組成物およびキット
CN112680797B (zh) 一种去除高丰度rna的测序文库及其构建方法
US20180044724A1 (en) Dna ligase mediated dna amplification
JPS63500006A (ja) エキソヌクレア−ゼ阻害による核酸の塩基配列決定法
CN107002290B (zh) 样品制备方法
US20240043924A1 (en) Determining long dna sequence using short mps reads
CN115279918A (zh) 用于测序的新型核酸模板结构
CN113490750A (zh) 微量dna甲基化高通量测序方法
CN118019856A (zh) 缺口-连接stlfr