JP2022023100A5 - - Google Patents

Download PDF

Info

Publication number
JP2022023100A5
JP2022023100A5 JP2021164668A JP2021164668A JP2022023100A5 JP 2022023100 A5 JP2022023100 A5 JP 2022023100A5 JP 2021164668 A JP2021164668 A JP 2021164668A JP 2021164668 A JP2021164668 A JP 2021164668A JP 2022023100 A5 JP2022023100 A5 JP 2022023100A5
Authority
JP
Japan
Prior art keywords
nucleic acid
acid molecule
detectable label
attachment
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2021164668A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022023100A (ja
JP7244601B2 (ja
Filing date
Publication date
Priority claimed from JP2017527209A external-priority patent/JP6674951B2/ja
Application filed filed Critical
Publication of JP2022023100A publication Critical patent/JP2022023100A/ja
Publication of JP2022023100A5 publication Critical patent/JP2022023100A5/ja
Priority to JP2023036774A priority Critical patent/JP7569404B2/ja
Application granted granted Critical
Publication of JP7244601B2 publication Critical patent/JP7244601B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2021164668A 2014-11-21 2021-10-06 酵素不要及び増幅不要の配列決定 Active JP7244601B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023036774A JP7569404B2 (ja) 2014-11-21 2023-03-09 酵素不要及び増幅不要の配列決定

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462082883P 2014-11-21 2014-11-21
US62/082,883 2014-11-21
JP2017527209A JP6674951B2 (ja) 2014-11-21 2015-11-19 酵素不要及び増幅不要の配列決定
JP2020040296A JP6959378B2 (ja) 2014-11-21 2020-03-09 酵素不要及び増幅不要の配列決定

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2020040296A Division JP6959378B2 (ja) 2014-11-21 2020-03-09 酵素不要及び増幅不要の配列決定

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023036774A Division JP7569404B2 (ja) 2014-11-21 2023-03-09 酵素不要及び増幅不要の配列決定

Publications (3)

Publication Number Publication Date
JP2022023100A JP2022023100A (ja) 2022-02-07
JP2022023100A5 true JP2022023100A5 (https=) 2022-08-04
JP7244601B2 JP7244601B2 (ja) 2023-03-22

Family

ID=54705918

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2017527209A Active JP6674951B2 (ja) 2014-11-21 2015-11-19 酵素不要及び増幅不要の配列決定
JP2020040296A Active JP6959378B2 (ja) 2014-11-21 2020-03-09 酵素不要及び増幅不要の配列決定
JP2021164668A Active JP7244601B2 (ja) 2014-11-21 2021-10-06 酵素不要及び増幅不要の配列決定
JP2023036774A Active JP7569404B2 (ja) 2014-11-21 2023-03-09 酵素不要及び増幅不要の配列決定

Family Applications Before (2)

Application Number Title Priority Date Filing Date
JP2017527209A Active JP6674951B2 (ja) 2014-11-21 2015-11-19 酵素不要及び増幅不要の配列決定
JP2020040296A Active JP6959378B2 (ja) 2014-11-21 2020-03-09 酵素不要及び増幅不要の配列決定

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2023036774A Active JP7569404B2 (ja) 2014-11-21 2023-03-09 酵素不要及び増幅不要の配列決定

Country Status (10)

Country Link
US (4) US20160194701A1 (https=)
EP (4) EP4029952B1 (https=)
JP (4) JP6674951B2 (https=)
KR (4) KR102738175B1 (https=)
CN (2) CN107208144B (https=)
AU (3) AU2015349870B2 (https=)
CA (1) CA2968376C (https=)
ES (2) ES2770055T3 (https=)
SG (1) SG11201704098VA (https=)
WO (1) WO2016081740A1 (https=)

Families Citing this family (108)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3604555B1 (en) * 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequencing by structure assembly
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
EP4249605B1 (en) 2011-12-22 2024-08-28 President And Fellows Of Harvard College Methods for analyte detection
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
EP2971184B1 (en) 2013-03-12 2019-04-17 President and Fellows of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
SG10201913068PA (en) 2013-06-04 2020-02-27 Harvard College Rna-guided transcriptional regulation
CA2916662C (en) 2013-06-25 2022-03-08 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
EP4328322A3 (en) 2014-07-30 2024-05-22 President and Fellows of Harvard College Probe library construction
EP3901282B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
SG10202107053QA (en) 2015-07-17 2021-08-30 Nanostring Technologies Inc Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
WO2017015097A1 (en) 2015-07-17 2017-01-26 Nanostring Technologies, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
AU2016349288A1 (en) 2015-11-03 2018-05-31 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
EP4613756A3 (en) 2016-04-25 2025-11-12 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
CN116200462B (zh) * 2016-05-16 2025-10-03 布鲁克空间生物学公司 用于检测样品中目标核酸的方法
US11352667B2 (en) 2016-06-21 2022-06-07 10X Genomics, Inc. Nucleic acid sequencing
CN118853848A (zh) 2016-08-31 2024-10-29 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
CN118389650A (zh) 2016-08-31 2024-07-26 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
EP4556575A3 (en) 2016-11-21 2025-10-08 Bruker Spatial Biology, Inc. A method for sequencing nucleic acids
CN110291207A (zh) * 2017-01-27 2019-09-27 豪夫迈·罗氏有限公司 用于长程测序的条形码化dna
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
WO2019069371A1 (ja) * 2017-10-03 2019-04-11 株式会社ニコン 検体中の検出対象の検出方法、検出キット及び流体デバイス
CN120210336A (zh) 2017-10-06 2025-06-27 10X基因组学有限公司 Rna模板化连接
CN110079592B (zh) * 2018-01-26 2021-02-12 厦门艾德生物医药科技股份有限公司 用于检测基因突变和已知、未知基因融合类型的高通量测序靶向捕获目标区域的探针和方法
US20190249248A1 (en) 2018-02-12 2019-08-15 Nanostring Technologies, Inc. Biomolecular probes and methods of detecting gene and protein expression
CA3093846A1 (en) 2018-03-15 2019-09-19 Twinstrand Biosciences, Inc. Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations
EP3794146B1 (en) 2018-05-14 2025-12-10 Bruker Spatial Biology, Inc. Method for identifying a predetermined nucleotide sequence
CN112770776B (zh) 2018-07-30 2025-08-19 瑞德库尔有限责任公司 用于样品处理或分析的方法和系统
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
EP3894583A4 (en) 2018-12-13 2022-11-09 President and Fellows of Harvard College AMPLIFICATION METHODS AND SYSTEMS FOR MERFISH AND OTHER APPLICATIONS
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN119220641A (zh) 2019-05-31 2024-12-31 10X基因组学有限公司 检测目标核酸分子的方法
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021127637A1 (en) * 2019-12-19 2021-06-24 Akoya Biosciences, Inc. Rna detection
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
CN115023502A (zh) * 2019-12-30 2022-09-06 哈佛学院院长及董事 染色质的3d组织和转录活动的基因组规模成像
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
EP4097251A1 (en) 2020-01-29 2022-12-07 10X Genomics, Inc. Compositions and methods for analyte detection
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN115485391A (zh) 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
EP4484571A3 (en) 2020-02-21 2025-03-19 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
WO2021225900A1 (en) * 2020-05-04 2021-11-11 10X Genomics, Inc. Spatial transcriptomic transfer modes
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4153776B1 (en) 2020-05-22 2025-03-05 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207B1 (en) 2020-06-10 2024-09-25 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
US12492427B2 (en) 2020-06-18 2025-12-09 Resolve Biosciences Gmbh Multiplex method for detecting different analytes in a sample
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US12509717B2 (en) 2020-12-11 2025-12-30 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
AU2022317053B2 (en) 2021-07-30 2025-01-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12553079B2 (en) 2021-08-03 2026-02-17 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
EP4446426B1 (en) 2021-08-16 2026-03-04 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
CN118974836A (zh) 2022-03-08 2024-11-15 10X基因组学有限公司 将光学拥挤最小化的原位代码设计方法
EP4505177A1 (en) 2022-04-01 2025-02-12 10x Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
WO2023215603A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
US20240084378A1 (en) 2022-05-11 2024-03-14 10X Genomics, Inc. Compositions and methods for in situ sequencing
EP4540607A1 (en) 2022-06-17 2025-04-23 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2024040060A1 (en) 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
EP4482979B1 (en) 2022-11-09 2025-10-01 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
EP4680764A2 (en) 2023-03-17 2026-01-21 Bruker Spatial Biology, Inc. Assay for recombinase accessible chromatin and related compositions and methods
WO2024196728A2 (en) 2023-03-17 2024-09-26 Nanostring Technologies, Inc. Nucleic acid probes for combined sequencing and spatial analysis
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4230558A (en) 1978-10-02 1980-10-28 Coulter Electronics, Inc. Single drop separator
US4318846A (en) 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US5091519A (en) 1986-05-01 1992-02-25 Amoco Corporation Nucleotide compositions with linking groups
US4811218A (en) 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5066580A (en) 1988-08-31 1991-11-19 Becton Dickinson And Company Xanthene dyes that emit to the red of fluorescein
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5847162A (en) 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US6322901B1 (en) 1997-11-13 2001-11-27 Massachusetts Institute Of Technology Highly luminescent color-selective nano-crystalline materials
US6207392B1 (en) 1997-11-25 2001-03-27 The Regents Of The University Of California Semiconductor nanocrystal probes for biological applications and process for making and using such probes
US5990479A (en) 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US6251303B1 (en) 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6426513B1 (en) 1998-09-18 2002-07-30 Massachusetts Institute Of Technology Water-soluble thiol-capped nanocrystals
DE60023480T2 (de) * 1999-04-08 2006-07-20 Gen-Probe Inc., San Diego Amplifikation und sequenzierung von primer-paaren und deren verwendung
US6649138B2 (en) 2000-10-13 2003-11-18 Quantum Dot Corporation Surface-modified semiconductive and metallic nanoparticles having enhanced dispersibility in aqueous media
US6576291B2 (en) 2000-12-08 2003-06-10 Massachusetts Institute Of Technology Preparation of nanocrystallites
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
JP4567436B2 (ja) 2001-07-20 2010-10-20 ライフ テクノロジーズ コーポレーション 発光ナノ粒子およびそれらの調製方法
JP4283578B2 (ja) * 2003-03-31 2009-06-24 学校法人片柳学園 不一致増幅物質を含むプローブ核酸を用いる標的配列の検出方法、プローブ核酸および標的配列検出用アッセイキット
JP2008512129A (ja) * 2004-09-10 2008-04-24 セクエノム,インコーポレイティド 核酸の広範囲配列分析法
JP4839051B2 (ja) * 2004-10-08 2011-12-14 シスメックス株式会社 核酸プローブを用いる被検物質の検出方法
EP1954708A4 (en) 2005-11-23 2009-05-13 Univ Utah Res Found Methods and compositions involving intrinsic genes
WO2007076128A2 (en) 2005-12-23 2007-07-05 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
JP5700911B2 (ja) 2005-12-23 2015-04-15 ナノストリング テクノロジーズ,インコーポレーテッド 配向され、固定化された巨大分子を含む組成物とその製造法
CN101432439B (zh) * 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
CA2653095C (en) 2006-05-22 2013-07-16 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
US7745129B1 (en) * 2006-07-12 2010-06-29 Kenneth David Schatz Methods for sequencing of a necleic acid
US20080269068A1 (en) * 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
CA2687292C (en) 2007-04-10 2017-07-04 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
ES2457534T3 (es) 2008-05-30 2014-04-28 The University Of North Carolina At Chapel Hill Perfiles de expresión génica para predecir desenlaces en cáncer de mama
US8519115B2 (en) * 2008-08-14 2013-08-27 Nanostring Technologies, Inc. Stable nanoreporters
SG174617A1 (en) * 2009-04-02 2011-10-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
WO2011047087A2 (en) 2009-10-13 2011-04-21 Nanostring Technologies, Inc. Protein detection via nanoreporters
EP2516681B1 (en) 2010-02-11 2017-10-18 Nanostring Technologies, Inc Compositions and methods for the detection of preferably small rnas by bridge hybridisation and ligation
US20110229888A1 (en) 2010-03-16 2011-09-22 Hengen Paul N Compositions and Methods for the Detection of Genomic Features
EP2685988A4 (en) 2011-03-15 2014-08-20 Univ North Carolina Methods of treating breast cancer with anthracycline therapy
US9758834B2 (en) 2011-03-28 2017-09-12 Nanostring Technologies, Inc. Compositions and methods for diagnosing cancer
US8809238B2 (en) * 2011-05-09 2014-08-19 Fluidigm Corporation Probe based nucleic acid detection
CA2839705A1 (en) 2011-06-24 2012-12-27 Nanostring Technologies, Inc. Multivariate diagnostic assays and methods for using same
WO2013040257A1 (en) * 2011-09-13 2013-03-21 Lasergen, Inc. 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
EP3604555B1 (en) * 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequencing by structure assembly
JP6144695B2 (ja) 2011-11-30 2017-06-07 ユニバーシティー オブ ノースカロライナ アット チャペル ヒル タキサン療法を用いて乳癌を処置する方法
US10385392B2 (en) * 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes
CA2874492C (en) 2012-05-22 2021-10-19 British Columbia Cancer Agency Branch Nano46 genes and methods to predict breast cancer outcome
US20140037620A1 (en) 2012-06-29 2014-02-06 British Columbia Cancer Agency Branch Methods of Treating Breast Cancer with Gemcitabine Therapy
WO2014075067A1 (en) 2012-11-12 2014-05-15 Nanostring Technologies, Inc. Methods to predict breast cancer outcome
CA2907493A1 (en) * 2013-04-30 2014-11-13 California Institute Of Technology Multiplex labeling of molecules by sequential hybridization barcoding
EP4556575A3 (en) * 2016-11-21 2025-10-08 Bruker Spatial Biology, Inc. A method for sequencing nucleic acids
EP3794146B1 (en) * 2018-05-14 2025-12-10 Bruker Spatial Biology, Inc. Method for identifying a predetermined nucleotide sequence

Similar Documents

Publication Publication Date Title
JP2022023100A5 (https=)
JP2017535269A5 (https=)
AU2022271472B2 (en) Enzyme- and amplification-free sequencing
US12534754B2 (en) Attenuators
US7914989B2 (en) Capture moieties for nucleic acids and uses thereof
US9957550B2 (en) Attenuators
IL152727A (en) Single copy genomic hybridization probes and method of generating same
US20160251712A1 (en) Sequential Sequencing
JP2021523723A5 (https=)
JP2015511819A5 (https=)
KR20020008195A (ko) 폴리뉴클레오티드 서열 변형의 미세배열-기초 분석
CN101142325A (zh) 制备用于分析的多核苷酸的方法
WO2015014962A1 (en) Sequence capture method using specialized capture probes (heatseq)
JP2022502343A (ja) 肝癌によく見られた複数の変異を同時に検出するctDNAライブラリーの構築及びシークエンシングデータの分析の方法
JP2022540716A5 (https=)
JP2022519324A5 (https=)
WO2010133257A1 (en) Method for detection and identification of bacterial strains belonging to the classes escherichia coli, salmonella, campylobacter and listeria
RU2001114072A (ru) Диагностический анализ
JP2020516232A5 (https=)
CN109295048A (zh) 一种全基因组分子标记检测的方法
Böhme et al. Detection of food spoilage and pathogenic bacteria based on ligation detection reaction coupled to flow‐through hybridization on membranes
CN110592260B (zh) 硬粒小麦成株抗叶锈病位点的竞争性等位基因特异聚合酶链式反应标记及其应用
WO2015071552A1 (en) Multi-unit probes with high specificity and a method of designing the same
WO2007095055A2 (en) Methods and compositions for detecting viral nucleic acid in a cell
JP7589951B2 (ja) プローブ誘導ヘテロ二重鎖の移動度アッセイ