JP2021508484A5 - - Google Patents
Info
- Publication number
- JP2021508484A5 JP2021508484A5 JP2020536656A JP2020536656A JP2021508484A5 JP 2021508484 A5 JP2021508484 A5 JP 2021508484A5 JP 2020536656 A JP2020536656 A JP 2020536656A JP 2020536656 A JP2020536656 A JP 2020536656A JP 2021508484 A5 JP2021508484 A5 JP 2021508484A5
- Authority
- JP
- Japan
- Prior art keywords
- buffer
- approximately
- affinity resin
- washing step
- elution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2023072157A JP7531654B2 (ja) | 2017-12-29 | 2023-04-26 | アデノ随伴ウイルス精製方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762611709P | 2017-12-29 | 2017-12-29 | |
| US62/611,709 | 2017-12-29 | ||
| PCT/US2018/067627 WO2019133677A1 (en) | 2017-12-29 | 2018-12-27 | Adeno-associated virus purification methods |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023072157A Division JP7531654B2 (ja) | 2017-12-29 | 2023-04-26 | アデノ随伴ウイルス精製方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021508484A JP2021508484A (ja) | 2021-03-11 |
| JP2021508484A5 true JP2021508484A5 (enExample) | 2022-02-08 |
| JP7299896B2 JP7299896B2 (ja) | 2023-06-28 |
Family
ID=67064154
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020536656A Active JP7299896B2 (ja) | 2017-12-29 | 2018-12-27 | アデノ随伴ウイルス精製方法 |
| JP2023072157A Active JP7531654B2 (ja) | 2017-12-29 | 2023-04-26 | アデノ随伴ウイルス精製方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023072157A Active JP7531654B2 (ja) | 2017-12-29 | 2023-04-26 | アデノ随伴ウイルス精製方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US11981934B2 (enExample) |
| EP (1) | EP3731863A4 (enExample) |
| JP (2) | JP7299896B2 (enExample) |
| KR (1) | KR20200106045A (enExample) |
| CN (1) | CN111655285B (enExample) |
| AU (1) | AU2018395254B2 (enExample) |
| BR (1) | BR112020013197A8 (enExample) |
| CA (1) | CA3084827A1 (enExample) |
| IL (1) | IL275533B2 (enExample) |
| MX (1) | MX2020006884A (enExample) |
| SG (1) | SG11202006232SA (enExample) |
| WO (1) | WO2019133677A1 (enExample) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20210032984A (ko) * | 2018-07-11 | 2021-03-25 | 박스알타 인코퍼레이티드 | Aav 조성물 |
| CN114173827B (zh) * | 2019-06-28 | 2025-01-28 | 武田药品工业株式会社 | 腺相关病毒纯化方法 |
| EP3868886A1 (en) * | 2020-02-21 | 2021-08-25 | Bia Separations D.O.O. | A method for separation or depletion of empty aav capsids from full aav capsids |
| US20220033782A1 (en) * | 2020-07-29 | 2022-02-03 | Pall Corporation | Adenovirus-associated viruses separation method |
| US20230302381A1 (en) * | 2020-08-18 | 2023-09-28 | Sartorius Bia Separations D.O.O. | Metal-affinity extraction of host cell dna |
| AU2021375404A1 (en) * | 2020-11-03 | 2023-06-08 | Pfizer Inc. | Methods for purification of aav vectors by anion exchange chromatography |
| EP4251756A1 (en) * | 2020-11-24 | 2023-10-04 | Sartorius BIA Separations d.o.o. | Improved washing methods for affinity chromatography |
| IL303959A (en) * | 2020-12-23 | 2023-08-01 | Pfizer | Methods for purification of aav vectors by affinity chromatography |
| US20240084268A1 (en) * | 2021-01-21 | 2024-03-14 | Asklepios Biopharmaceutical, Inc. | Method for purifying recombinant viral particles |
| CN114591921B (zh) * | 2022-03-31 | 2024-08-02 | 上海勉亦生物科技有限公司 | 腺相关病毒的纯化方法和洗脱液 |
| WO2022222869A1 (zh) * | 2021-04-19 | 2022-10-27 | 信念医药（香港）有限公司 | 重组腺相关病毒及其应用 |
| GB202110014D0 (en) * | 2021-07-12 | 2021-08-25 | Cytiva Bioprocess R & D Ab | A method for separating adeno-associated virus capsids, compositions obtained by said method and uses thereof |
| CN113552349B (zh) * | 2021-07-30 | 2022-07-19 | 上海勉亦生物科技有限公司 | Aav蛋白外壳的检测方法 |
| WO2023077086A1 (en) * | 2021-10-29 | 2023-05-04 | Homology Medicines, Inc. | Methods and compositions for the purification of adeno-associated virus |
| CN114350622A (zh) * | 2022-01-17 | 2022-04-15 | 苏州博腾生物制药有限公司 | 一种病毒纯化高效保护试剂及其应用 |
| MX2024010855A (es) | 2022-03-07 | 2024-09-11 | Takeda Pharmaceuticals Co | Produccion cromatografica de afinidad de productos clinicos de inmunoglobulina g (igg) humana. |
| GB202206123D0 (en) * | 2022-04-27 | 2022-06-08 | Cytiva Bioprocess R & D Ab | A pre-screening method and a method for separating adeno-associated virus capsids |
| WO2024233992A2 (en) * | 2023-05-11 | 2024-11-14 | Trophogen, Inc. | Methods of manufacturing highly glycosylated glycoprotein hormones and their analogs |
| KR20260049579A (ko) * | 2023-08-01 | 2026-04-14 | 메르크 파텐트 게엠베하 | 개선된 용리 시스템의 음이온 교환 크로마토그래피를 사용한 aav 입자의 연마 |
| WO2025104341A1 (en) * | 2023-11-16 | 2025-05-22 | Sartorius Bia Separations D.O.O. | Enhanced chromatographic separation of components in a mixture by employing decreasing gradient of complexing agent |
| WO2025207456A1 (en) * | 2024-03-25 | 2025-10-02 | Frontera Therapeutics, Inc. | Methods for purification of adeno associated virus particles by anion exchange chromatography |
| WO2026077794A1 (en) * | 2024-10-07 | 2026-04-16 | Cytiva Us Llc | Plasmid purification methods |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4543363A (en) | 1983-06-15 | 1985-09-24 | Asahi Kasei Kogyo Kabushiki Kaisha | Ion exchanger having hydroxyl groups bonded directly to backbone skeleton |
| SE9902133D0 (sv) | 1999-06-06 | 1999-06-06 | Amersham Pharm Biotech Ab | A method for surface modification, a novel support matrix and the use of the matrix |
| GB9925432D0 (en) | 1999-10-27 | 1999-12-29 | Univ London | Virus preparation |
| US6593123B1 (en) * | 2000-08-07 | 2003-07-15 | Avigen, Inc. | Large-scale recombinant adeno-associated virus (rAAV) production and purification |
| KR20180000341A (ko) * | 2009-06-16 | 2018-01-02 | 젠자임 코포레이션 | 재조합 ａａｖ 벡터에 대한 개선된 정제 방법 |
| WO2014066471A1 (en) | 2012-10-24 | 2014-05-01 | Genzyme Corporation | Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers |
| CN104232687A (zh) | 2014-09-17 | 2014-12-24 | 许瑞安 | 一种重组腺相关病毒rAAV载体的分离纯化方法 |
| EP3054007A1 (en) * | 2015-02-09 | 2016-08-10 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step |
| EP3387138B1 (en) | 2015-12-11 | 2022-01-26 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav9 |
| ES2934848T3 (es) * | 2015-12-11 | 2023-02-27 | Univ Pennsylvania | Método de purificación escalable para AAV8 |
| WO2021101987A1 (en) | 2019-11-21 | 2021-05-27 | Emd Millipore Corporation | Hydrophilic membranes |
-
2018
- 2018-12-27 EP EP18895008.3A patent/EP3731863A4/en active Pending
- 2018-12-27 BR BR112020013197A patent/BR112020013197A8/pt unknown
- 2018-12-27 WO PCT/US2018/067627 patent/WO2019133677A1/en not_active Ceased
- 2018-12-27 AU AU2018395254A patent/AU2018395254B2/en active Active
- 2018-12-27 IL IL275533A patent/IL275533B2/en unknown
- 2018-12-27 CN CN201880087932.0A patent/CN111655285B/zh active Active
- 2018-12-27 MX MX2020006884A patent/MX2020006884A/es unknown
- 2018-12-27 KR KR1020207021627A patent/KR20200106045A/ko not_active Ceased
- 2018-12-27 SG SG11202006232SA patent/SG11202006232SA/en unknown
- 2018-12-27 CA CA3084827A patent/CA3084827A1/en active Pending
- 2018-12-27 JP JP2020536656A patent/JP7299896B2/ja active Active
- 2018-12-27 US US16/958,974 patent/US11981934B2/en active Active
-
2023
- 2023-04-26 JP JP2023072157A patent/JP7531654B2/ja active Active
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